Origin IGS: aaaaaaccgctaaatcgaaaaaaaattgcgcctagtttaccttgaatcgtatcaaaatgcgacattgcccgcccggtgcggaataatttcttaaaaattgggttatatcgtagtaagataaggcaattttgacagcctgagaggtgagacc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ggtctcacctctcaggctgtcaaaattgccttatcttactacgatataacccaatttttaagaaattattccgcaccgggcgggcaatgtcgcattttgatacgattcaaggtaaactaggcgcaattttttttcgatttagcggtttttt Mask Tandem Repeat Region ================================================ aaaaaaccgctaaatcgaaaaaaaattgcgcctagtttaccttgaatcgtatcaaaatgcgacattgcccgcccggtgcggaataatttcttaaaaattgggttatatcgtagtaagataaggcaattttgacagcctgagaggtgagacc Find is-nt database================================================ Query_seq: AA01418:AA01419|AA01418:AA01419:conserved hypothetical protein:D-ribulose-phosphate-3 epimerase:->->:954367..954517 151 aaaaaaccgctaaatcgaaaaaaaattgcgcctagtttaccttgaatcgtatcaaaatgcgacattgcccgcccggtgcggaataatttcttaaaaattgggttatatcgtagtaagataaggcaattttgacagcctgagaggtgagacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01418:AA01419|AA01418:AA01419:conserved hypothetical protein:D-ribulose-phosphate-3 epimerase:->->:954367..954517 151 aaaaaaccgctaaatcgaaaaaaaattgcgcctagtttaccttgaatcgtatcaaaatgcgacattgcccgcccggtgcggaataatttcttaaaaattgggttatatcgtagtaagataaggcaattttgacagcctgagaggtgagacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01418:AA01419|AA01418:AA01419:conserved hypothetical protein:D-ribulose-phosphate-3 epimerase:->->:954367..954517 151 aaaaaaccgctaaatcgaaaaaaaattgcgcctagtttaccttgaatcgtatcaaaatgcgacattgcccgcccggtgcggaataatttcttaaaaattgggttatatcgtagtaagataaggcaattttgacagcctgagaggtgagacc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaaaaccgctaaatcgaaaaaaaattgcgcctagtttaccttgaatcgtatcaaaatgcgacattgcccgcccggtgcggaataatttcttaaaaattgggttatatcgtagtaagataaggcaattttgacagcctgagaggtgagacc Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: - Start: 35 End: 127 .................................. N V K F R I L I R C Q G G P A S Y N R L F Q T I D Y Y S L A M ........................ Protein_Len: 39 Strand: - Start: 3 End: 119 .. F G S F R F F I A G L K G Q I T D F H S M A R G T R F L K K F I P N Y R L L M ................................ aaaaaaccgctaaatcgaaaaaaaattgcgcctagtttaccttgaatcgtatcaaaatgcgacattgcccgcccggtgcggaataatttcttaaaaattgggttatatcgtagtaagataaggcaattttgacagcctgagaggtgagacc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 90 End: 119 .........................................................................................CTTAAAAATTGGGTTATATCGTAGTAAGAT................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaaaaccgctaaatcgaaaaaaaattgcgcctagtttaccttgaatcgtatcaaaatgcgacattgcccgcccggtgcggaataatttcttaaaaattgggttatatcgtagtaagataaggcaattttgacagcctgagaggtgagacc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01418:AA01419|AA01418:AA01419:conserved hypothetical protein:D-ribulose-phosphate-3 epimerase:->->:954367..954517 151 aaaaaaccgctaaatcgaaaaaaaattgcgcctagtttaccttgaatcgtatcaaaatgcgacattgcccgcccggtgcggaataatttcttaaaaattgggttatatcgtagtaagataaggcaattttgacagcctgagaggtgagacc Intra-Species Hit: Count: 1 Min: 1 Max: 151 Len: 151 Subject: aact_AA01418_AA01419|conserved hypothetical protein:D-ribulose-phosphate-3 epimerase|POSITIVE:POSITIVE|[954367,954517]|151 HSP 1 e-value: 3.0E-66 bit: 252.0 Len: 151 Query Start:1 Query End:151 Subject Strand: POSITIVE Subject Start: 1 Subject End: 151 aaaaaaccgctaaatcgnnnnnnnnttgcgcctagtttaccttgaatcgtatcaaaatgcgacattgcccgcccggtgcggaataatttcttaaaaattgggttatatcgtagtaagataaggcaattttgacagcctgagaggtgagacc aaaaaaccgctaaatcgaaaaaaaattgcgcctagtttaccttgaatcgtatcaaaatgcgacattgcccgcccggtgcggaataatttcttaaaaattgggttatatcgtagtaagataaggcaattttgacagcctgagaggtgagacc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAAACCGCUAAAUCGAAAAAAAAUUGCGCCUAGUUUACCUUGAAUCGUAUCAAAAUGCGACAUUGCCCGCCCGGUGCGGAAUAAUUUCUUAAAAAUUGGGUUAUAUCGUAGUAAGAUAAGGCAAUUUUGACAGCCUGAGAGGUGAGACC .......(((.................))).....((((((((...((((((....)))))).((((((.(((((((..((((....)))).....)))))))..((((.......)))).)))))).............))))))))... (-31.13) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUCUCACCUCUCAGGCUGUCAAAAUUGCCUUAUCUUACUACGAUAUAACCCAAUUUUUAAGAAAUUAUUCCGCACCGGGCGGGCAAUGUCGCAUUUUGAUACGAUUCAAGGUAAACUAGGCGCAAUUUUUUUUCGAUUUAGCGGUUUUUU ((.....))......((((((((((((((((((..((((..((((((..(((......(((....)))..(((...)))..)))..))))))....((((......)))).))))..)))).)))))))).....)))..)))........ (-30.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 438 83 43 44 2 aact:AA02358|5end_homoserine_kinase_1617128..1617358_POSITIVE 362 77 36 178 119 aact:AA00738|5end_hypothetical_protein_505087..505317_POSITIVE 359 80 31 196 156 aact:AA00374|5end_short-chain_fatty_acids_transporter_251681..251911_POSITIVE 357 79 40 112 72 aact:AA00329|5end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_222737..222967_POSITIVE 349 93 52 225 181 aact:AA01592|5end_hypothetical_protein_1070950..1071180_POSITIVE 345 80 40 205 158 aact:AA01914|5end_conserved_hypothetical_protein_1290126..1290356_POSITIVE 344 109 65 70 19 aact:AA01827|5end_iron(III)_ABC_transporter,_periplasmic_binding_protein_1230431..1230661_POSITIVE 343 100 52 192 142 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 334 79 34 130 75 aact:AA01628|5end_thiamin-monophosphate_kinase_1100888..1101118_POSITIVE 332 80 31 98 33 aact:AA00425|5end_N-terminal_region_of_competence_protein_M_287295..287525_POSITIVE 330 110 66 175 117 aact:AA00725|5end_translation_initiation_factor_1_497981..498211_POSITIVE 324 80 31 201 150 aact:AA00790|5end_recombination_DNA_repair_protein_542455..542685_POSITIVE 323 80 31 165 109 aact:AA01018|5end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_711663..711893_POSITIVE 319 90 42 200 156 aact:AA02405|5end_conserved_hypothetical_protein_1652430..1652660_POSITIVE 319 90 42 148 104 aact:AA02404|5end_hypothetical_protein_1652378..1652608_POSITIVE 319 93 59 41 2 aact:AA01590|5end_hypothetical_protein_1070766..1070996_POSITIVE 313 70 22 198 157 aact:AA01062|5end_ribonuclease_PH_(RNase_PH)_735706..735936_POSITIVE 309 80 31 175 120 aact:AA00533|5end_conserved_hypothetical_protein_365323..365553_POSITIVE 305 79 46 79 42 aact:AA00574|5end_regulatory_protein_392113..392343_POSITIVE 303 83 49 216 179 aact:AA01313|5end_deoxyuridinetriphosphatase;_deoxyuridine_5'-triphosphate_nucleotidohydrolase_889567..889797_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 372 79 32 63 14 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 349 93 52 66 22 aact:AA01589|3end_conserved_hypothetical_protein_1070871..1071021_POSITIVE 343 100 52 97 47 aact:AA00633|3end_hypothetical_protein_435167..435317_POSITIVE 334 79 34 108 53 aact:AA01626|3end_N_utilization_substance_protein_B_1100946..1101096_POSITIVE 332 80 31 126 61 aact:AA00424|3end_hypothetical_protein_287403..287553_POSITIVE 330 110 66 150 92 aact:AA00724|3end_hypothetical_protein_498036..498186_POSITIVE 323 82 41 136 96 aact:AA01062|3end_ribonuclease_PH_(RNase_PH)_736499..736649_POSITIVE 323 80 31 148 92 aact:AA01017|3end_conserved_hypothetical_protein_711726..711876_POSITIVE 319 90 42 132 88 aact:AA02402|3end_O-sialoglycoprotein_endopeptidase_1652442..1652592_POSITIVE 317 77 35 42 5 aact:AA01817|3end_hypothetical_protein_1224400..1224550_POSITIVE 314 80 35 112 65 aact:AA01467|3end_mannose-specific_phosphotransferase_system_IID_987214..987364_POSITIVE 308 79 36 150 95 aact:AA00737|3end_citrate_lyase,_alpha_chain_(citrase)_505143..505293_POSITIVE 301 81 60 148 117 aact:AA02762|3end_ribonuclease_H_1933708..1933858_POSITIVE 297 83 60 28 1 aact:AA02357|3end_bifunctional_protein:_aspartokinase_I;_homoserine_dehydrogenaseI_1617192..1617342_POSITIVE 297 81 41 150 102 aact:AA01137|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_A_781057..781207_POSITIVE 297 81 41 119 75 aact:AA01069|3end_translation_elongation_factor_(EF-Ts)_740700..740850_POSITIVE 296 87 59 55 16 aact:AA01450|3end_hypothetical_protein_972032..972182_POSITIVE 295 79 31 146 86 aact:AA02177|3end_conserved_hypothetical_protein_1489432..1489582_POSITIVE 295 81 41 117 73 aact:AA01679|3end_aspartate-semialdehyde_dehydrogenase;_PTS_system_enzyme_II_A_component_1135236..1135386_POSITIVE 290 80 41 49 7 aact:AA00969|3end_conserved_hypothetical_protein_(possible_NADH_pyrophosphatase)_676206..676356_POSITIVE