Origin IGS: tgacctgctgcaagtgtccgaaatacctagttcaaccttttattatcaacagggcaggattacagccgggtaaatttggtgtattacaaccaaaacgaattaaacccgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtccgaaaaacacctaaatttctgaccgcactttttgttgtgttttgtcgatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatcgacaaaacacaacaaaaagtgcggtcagaaatttaggtgtttttcggaccgcactttgatctgcctcccaaaagttggacaggttagttaacttaagtattgagctcggtactgtaccgggctcaatacttttaaatcgggtttaattcgttttggttgtaatacaccaaatttacccggctgtaatcctgccctgttgataataaaaggttgaactaggtatttcggacacttgcagcaggtca Mask Tandem Repeat Region ================================================ tgacctgctgcaagtgtccgaaatacctagttcaaccttttattatcaacagggcaggattacagccgggtaaatttggtgtattacaaccaaaacgaattaaacccgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtccgaaaaacacctaaatttctgaccgcactttttgttgtgttttgtcgatta Find is-nt database================================================ Query_seq: AA01404:AA01407|AA01404:AA01407:hypothetical protein:outer membrane antigenic lipoprotein b precursor:->->:948895..949144 250 tgacctgctgcaagtgtccgaaatacctagttcaaccttttattatcaacagggcaggattacagccgggtaaatttggtgtattacaaccaaaacgaattaaacccgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtccgaaaaacacctaaatttctgaccgcactttttgttgtgttttgtcgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01404:AA01407|AA01404:AA01407:hypothetical protein:outer membrane antigenic lipoprotein b precursor:->->:948895..949144 250 tgacctgctgcaagtgtccgaaatacctagttcaaccttttattatcaacagggcaggattacagccgggtaaatttggtgtattacaaccaaaacgaattaaacccgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtccgaaaaacacctaaatttctgaccgcactttttgttgtgttttgtcgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01404:AA01407|AA01404:AA01407:hypothetical protein:outer membrane antigenic lipoprotein b precursor:->->:948895..949144 250 tgacctgctgcaagtgtccgaaatacctagttcaaccttttattatcaacagggcaggattacagccgggtaaatttggtgtattacaaccaaaacgaattaaacccgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtccgaaaaacacctaaatttctgaccgcactttttgttgtgttttgtcgatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgacctgctgcaagtgtccgaaatacctagttcaaccttttattatcaacagggcaggattacagccgggtaaatttggtgtattacaaccaaaacgaattaaacccgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtccgaaaaacacctaaatttctgaccgcactttttgttgtgttttgtcgatta Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 42 End: 155 ......................................... M I N R A G L Q P G K F G V L Q P K R I K P D L K V L S P V Q Y R A Q Y L S ............................................................................................... Protein_Len: 44 Strand: + Start: 119 End: 250 ...................................................................................................................... M E P G T V P S S I L K L T N L S N F W E A D Q S A V R K T P K F L T A L F V V F C R L tgacctgctgcaagtgtccgaaatacctagttcaaccttttattatcaacagggcaggattacagccgggtaaatttggtgtattacaaccaaaacgaattaaacccgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtccgaaaaacacctaaatttctgaccgcactttttgttgtgttttgtcgatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgacctgctgcaagtgtccgaaatacctagttcaaccttttattatcaacagggcaggattacagccgggtaaatttggtgtattacaaccaaaacgaattaaacccgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtccgaaaaacacctaaatttctgaccgcactttttgttgtgttttgtcgatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -17.9 Tail_Score: -3.19192 Start: 117 End: 149 Full_Region: AAACCCGATTTAAAA GTATTGAGCCCGGTA CAG TACCGAGCTCAATAC TTAAGTTAACTAACC ....................................................................................................................GTATTGAGCCCGGTACAGTACCGAGCTCAATAC..................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -20.1 Tail_Score: -4.20106 Start: 117 End: 149 Full_Region: ggttagttaacttaa gtattgagctcggta ctg taccgggctcaatac ttttaaatcgggttt ....................................................................................................................gtattgagctcggtactgtaccgggctcaatac..................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -9.1 Tail_Score: -5.35365 Start: 192 End: 227 Full_Region: GGGAGGCAGATCAAA GTGCGGTCCGAAA AACACCTAAA TTTCTGACCGCAC TTTTTGTTGTGTTTT ...............................................................................................................................................................................................GTGCGGTCCGAAAAACACCTAAATTTCTGACCGCAC....................... Find igs database================================================ Query_seq: AA01404:AA01407|AA01404:AA01407:hypothetical protein:outer membrane antigenic lipoprotein b precursor:->->:948895..949144 250 tgacctgctgcaagtgtccgaaatacctagttcaaccttttattatcaacagggcaggattacagccgggtaaatttggtgtattacaaccaaaacgaattaaacccgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtccgaaaaacacctaaatttctgaccgcactttttgttgtgttttgtcgatta Intra-Species Hit: Count: 2 Min: 1 Max: 250 Len: 250 Subject: aact_AA01404_AA01407|hypothetical protein:outer membrane antigenic lipoprotein b precursor|POSITIVE:POSITIVE|[948895,949144]|250 HSP 1 e-value: 1.0E-139 bit: 496.0 Len: 250 Query Start:1 Query End:250 Subject Strand: POSITIVE Subject Start: 1 Subject End: 250 tgacctgctgcaagtgtccgaaatacctagttcaaccttttattatcaacagggcaggattacagccgggtaaatttggtgtattacaaccaaaacgaattaaacccgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtccgaaaaacacctaaatttctgaccgcactttttgttgtgttttgtcgatta tgacctgctgcaagtgtccgaaatacctagttcaaccttttattatcaacagggcaggattacagccgggtaaatttggtgtattacaaccaaaacgaattaaacccgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtccgaaaaacacctaaatttctgaccgcactttttgttgtgttttgtcgatta Subject: aact_AA00631_AA00632|hypothetical protein:inner membrane protein ImpA|POSITIVE:POSITIVE|[433989,434266]|278 HSP 1 e-value: 7.0E-90 bit: 331.0 Len: 232 Query Start:1 Query End:232 Subject Strand: POSITIVE Subject Start: 23 Subject End: 258 tgacctgctgcaagtgtccgaaatacctagttcaaccttttattatcaacagg----gcaggattacagccgggtaaatttggtgtattacaaccaaaacgaattaaacccgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtccgaaaaacacctaaatttctgaccgcacttttt.................. tgacttgctgcaagtgtccgaaatacctcgttcaaccttttattatcaacaggttaagcaagattacagccgggtaaatttggtgtattaaaaccaaaacgaattaaactcgatttaaaagtattgagcccggtacagtaccgagctcaatacttaagttaactaacctgtccaacttttgggaggcagatcaaagtgcggtcgaaaaaagaattaaattttctaccgcacttttt.................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGACCUGCUGCAAGUGUCCGAAAUACCUAGUUCAACCUUUUAUUAUCAACAGGGCAGGAUUACAGCCGGGUAAAUUUGGUGUAUUACAACCAAAACGAAUUAAACCCGAUUUAAAAGUAUUGAGCCCGGUACAGUACCGAGCUCAAUACUUAAGUUAACUAACCUGUCCAACUUUUGGGAGGCAGAUCAAAGUGCGGUCCGAAAAACACCUAAAUUUCUGACCGCACUUUUUGUUGUGUUUUGUCGAUUA .(((((......)).)))((((((((.....((..(((((((.........(((((((........(((((...((((((........)))))).........)))))......(((((((((((.(((((...))))).)))))))))))...........)))))))......)))))))..))..(((((((((((.((((..........)))).))))))))))).....))))))))....... (-77.56) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCGACAAAACACAACAAAAAGUGCGGUCAGAAAUUUAGGUGUUUUUCGGACCGCACUUUGAUCUGCCUCCCAAAAGUUGGACAGGUUAGUUAACUUAAGUAUUGAGCUCGGUACUGUACCGGGCUCAAUACUUUUAAAUCGGGUUUAAUUCGUUUUGGUUGUAAUACACCAAAUUUACCCGGCUGUAAUCCUGCCCUGUUGAUAAUAAAAGGUUGAACUAGGUAUUUCGGACACUUGCAGCAGGUCA .....(((.......(((..(((((((((((.((((..........)))).)))))))))))((((((((...........)).)))))).))).....(((((((((((((((((...)))))))))))))))))......(((((.........((((((.(.....)))))))...)))))((((((((((((((..((((((........))).)))..))))....)))...)))))))..))). (-80.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 778 170 121 106 57 aact:AA01444|5end_ISRS011/IS150_related_transposase,_orfB_969159..969389_POSITIVE 778 170 121 106 57 aact:AA01287|5end_transposase_871583..871813_POSITIVE 751 170 121 150 101 aact:AA00226|5end_hypothetical_protein_156276..156506_POSITIVE 678 80 31 193 144 aact:AA00227|5end_hypothetical_sequence_156407..156637_POSITIVE 580 208 165 66 24 aact:AA01176|5end_hypothetical_protein_806309..806539_POSITIVE 559 199 164 141 105 aact:AA00145|5end_probable_degenerate_transposase_fragment_105046..105276_POSITIVE 542 198 165 81 48 aact:AA02681|5end_hypothetical_protein_1865749..1865979_POSITIVE 485 151 115 229 193 aact:AA00632|5end_inner_membrane_protein_ImpA_434127..434357_POSITIVE 472 198 164 74 36 aact:AA02419|5end_hypothetical_protein_1661731..1661961_POSITIVE 459 237 191 201 154 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 455 238 191 89 43 aact:AA02378|5end_hypothetical_protein_1627753..1627983_POSITIVE 452 210 165 155 112 aact:AA01267|5end_degenerate_transposase_fragment_862267..862497_POSITIVE 439 236 191 168 123 aact:AA02128|5end_DNA_topoisomerase_I_1451395..1451625_POSITIVE 439 230 181 213 164 aact:AA01145|5end_glycyl-tRNA_synthetase_alpha_subunit_783478..783708_POSITIVE 425 199 165 132 98 aact:AA01075|5end_hypothetical_protein_745605..745835_POSITIVE 424 230 181 141 94 aact:AA00126|5end_conserved_hypothetical_protein_91053..91283_POSITIVE 421 230 184 188 141 aact:AA02893|5end_oligopeptide_transport_system_permease_protein,_N-terminal_2027201..2027431_POSITIVE 412 230 181 154 96 aact:AA00660|5end_hypothetical_protein_453031..453261_POSITIVE 412 151 115 188 152 aact:AA00536|5end_5'-nucleotidase_precursor_366151..366381_POSITIVE 410 230 189 205 165 aact:AA02754|5end_conserved_hypothetical_protein_1928949..1929179_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 778 170 121 60 11 aact:AA01442|3end_phosphatidylserine_synthetase_969193..969343_POSITIVE 647 50 1 127 78 aact:AA00226|3end_hypothetical_protein_156451..156601_POSITIVE 580 208 165 72 30 aact:AA01175|3end_6-phosphogluconate_dehydrogenase_806395..806545_POSITIVE 542 198 165 63 30 aact:AA02679|3end_formate_dehydrogenase,_gamma_subunit_1865811..1865961_POSITIVE 472 198 164 74 36 aact:AA02418|3end_peptidyl-tRNA_hydrolase_1661811..1661961_POSITIVE 459 237 191 88 41 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 452 210 165 59 16 aact:AA01266|3end_hypothetical_protein_862251..862401_POSITIVE 442 237 195 143 100 aact:AA00528|3end_conserved_hypothetical_protein_361703..361853_POSITIVE 439 236 191 77 32 aact:AA02126|3end_conserved_hypothetical_protein_1451384..1451534_POSITIVE 436 238 191 98 48 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 430 199 164 60 30 aact:AA00221|3end_NADP-specific_glutamate_dehydrogenase_153865..154015_POSITIVE 425 199 165 70 36 aact:AA01074|3end_diacylglycerol_kinase_745623..745773_POSITIVE 421 230 184 84 37 aact:AA02892|3end_oligopeptide_ABC_transporter,_periplasmic-binding_protein_2027177..2027327_POSITIVE 412 230 181 70 12 aact:AA00659|3end_heat_shock_protein_453027..453177_POSITIVE 412 151 115 76 40 aact:AA00535|3end_hypothetical_protein_366119..366269_POSITIVE 410 237 191 84 40 aact:AA02781|3end_protein-export_membrane_protein_1945981..1946131_POSITIVE 410 230 189 44 4 aact:AA02751|3end_L-lactate_permease_1928868..1929018_POSITIVE 407 204 165 54 16 aact:AA02338|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1598885..1599035_POSITIVE 406 230 184 75 31 aact:AA02112|3end_cell_division_protein_1442184..1442334_POSITIVE 405 240 191 71 27 aact:AA01796|3end_branched-chain_amino_acid_aminotransferase_1212168..1212318_POSITIVE