Origin IGS: tagagtgaaaaagtgatctgcaccccaaaacctggacacctaatttgaggtgcagattatgtcttactcttatcaatttcgtttagaaattatcaaacaggtcacctagcaggattttggtatccgtgaggtggctgaacttcatcagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatgaaaaaaacgcaga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tctgcgtttttttcattttgggtttcagaattttggaggttttaggtttgtgtaaggtgattttacgccatcaagccttctttcacgaaaggctttaaccacaataaatgacttgagaatgaggaatctgatgaagttcagccacctcacggataccaaaatcctgctaggtgacctgtttgataatttctaaacgaaattgataagagtaagacataatctgcacctcaaattaggtgtccaggttttggggtgcagatcactttttcactcta Mask Tandem Repeat Region ================================================ tagagtgaaaaagtgatctgcaccccaaaacctggacacctaatttgaggtgcagattatgtcttactcttatcaatttcgtttagaaattatcaaacaggtcacctagcaggattttggtatccgtgaggtggctgaacttcatcagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatgaaaaaaacgcaga Find is-nt database================================================ Query_seq: AA01402:AA01404|AA01402:AA01404:beta 1-4 glucosyltransferase:hypothetical protein:->->:948443..948717 275 tagagtgaaaaagtgatctgcaccccaaaacctggacacctaatttgaggtgcagattatgtcttactcttatcaatttcgtttagaaattatcaaacaggtcacctagcaggattttggtatccgtgaggtggctgaacttcatcagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatgaaaaaaacgcaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01402:AA01404|AA01402:AA01404:beta 1-4 glucosyltransferase:hypothetical protein:->->:948443..948717 275 tagagtgaaaaagtgatctgcaccccaaaacctggacacctaatttgaggtgcagattatgtcttactcttatcaatttcgtttagaaattatcaaacaggtcacctagcaggattttggtatccgtgaggtggctgaacttcatcagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatgaaaaaaacgcaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01402:AA01404|AA01402:AA01404:beta 1-4 glucosyltransferase:hypothetical protein:->->:948443..948717 275 tagagtgaaaaagtgatctgcaccccaaaacctggacacctaatttgaggtgcagattatgtcttactcttatcaatttcgtttagaaattatcaaacaggtcacctagcaggattttggtatccgtgaggtggctgaacttcatcagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatgaaaaaaacgcaga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 14 Max: 145 Len: 132 Subject: UniRef90_Q7WRX1 Cluster: Hypothetical protein orf3; n=1; Haemophilus influenzae|Rep: Hypothetical protein orf3 - Haemophilus influenzae HSP 1 e-value: 3.0E-13 bit: 76.3 Len: 132 Query Start:14 Query End:145 Subject Strand: null Subject Start: 1 Subject End: 44 ............. M K F S H L T D T K I L L G D L F D N F * T K L I R V R H N L H L K L G V Q V L G C R S .................................................................................................................................. ............. M K F S H L T D T I I L F G D E F D N F Q T K L I R V G H N L H L K L G V Q I L G C S S .................................................................................................................................. tagagtgaaaaagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatgaaaaaaacgcaga Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: + Start: 114 End: 248 ................................................................................................................. M L V S V R W L N F I R F L I L K S F I V V K A F R E R R L D G V K S P Y T N L K P P K F ........................... tagagtgaaaaagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatgaaaaaaacgcaga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagagtgaaaaagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatgaaaaaaacgcaga Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 71 HP_score: -9.4 Tail_Score: -2.85763 Start: 17 End: 56 Full_Region: aagagtaagacataa tctgcacctcaaattagg tgt ccaggttttggggtgcaga tcactttttcactct ................tctgcacctcaaattaggtgtccaggttttggggtgcaga........................................................................................................................................................................................................................... TransTerm Strand: + Conf: 46 HP_score: -2.8 Tail_Score: -3.3573 Start: 127 End: 156 Full_Region: GGATTTTGGTATCCG TGAGGTGGCTGA ACTTCA TCAGATTCCTCA TTCTCAAGTCATTTA ..............................................................................................................................TGAGGTGGCTGAACTTCATCAGATTCCTCA....................................................................................................................... Find igs database================================================ Query_seq: AA01402:AA01404|AA01402:AA01404:beta 1-4 glucosyltransferase:hypothetical protein:->->:948443..948717 275 tagagtgaaaaagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatgaaaaaaacgcaga Intra-Species Hit: Count: 3 Min: 146 Max: 262 Len: 117 Subject: aact_AA01402_AA01404|beta 1-4 glucosyltransferase:hypothetical protein|POSITIVE:POSITIVE|[948443,948717]|275 HSP 1 e-value: 5.0E-60 bit: 232.0 Len: 117 Query Start:146 Query End:262 Subject Strand: POSITIVE Subject Start: 146 Subject End: 262 .................................................................................................................................................cagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatg............. .................................................................................................................................................cagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatg............. Subject: aact_AA00227_AA00229|hypothetical sequence:DNA polymerase III, alpha subunit|POSITIVE:POSITIVE|[156703,157002]|300 HSP 1 e-value: 1.0E-35 bit: 151.0 Len: 106 Query Start:157 Query End:262 Subject Strand: NEGATIVE Subject Start: 14 Subject End: 116 ............................................................................................................................................................ttctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatg............. ............................................................................................................................................................ttctcaagtcatttattgga---aaagcctttcgtgaaagagggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattatgaaacccaaaatg............. Subject: aact_AA01547_AA01549|deoxyribose-phosphate aldolase:hypothetical protein|POSITIVE:POSITIVE|[1037080,1037583]|504 HSP 1 e-value: 1.0E-32 bit: 141.0 Len: 117 Query Start:146 Query End:262 Subject Strand: POSITIVE Subject Start: 279 Subject End: 392 .................................................................................................................................................cagattcctcattctcaagtcatttattgtggttaaagcctttcgtgaaagaaggcttgatggcgtaaaatcaccttacacaaacctaaaacctccaaaattctgaaacccaaaatg............. .................................................................................................................................................cagatttctcgttctcaagtcatttattgga---aaagcctttcgtgaaagagggcttgatggcgtaaaatcgccttatacaaacctaaaacctccaaaattctgaaaccccaaatg............. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAGAUUCCUCAUUCUCAAGUCAUUUAUUGUGGUUAAAGCCUUUCGUGAAAGAAGGCUUGAUGGCGUAAAAUCACCUUACACAAACCUAAAACCUCCAAAAUUCUGAAACCCAAAAUG ((((...((........)).......(((((....((((((((.......))))))))...((.(......).))...)))))..................))))............ (-15.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CAUUUUGGGUUUCAGAAUUUUGGAGGUUUUAGGUUUGUGUAAGGUGAUUUUACGCCAUCAAGCCUUCUUUCACGAAAGGCUUUAACCACAAUAAAUGACUUGAGAAUGAGGAAUCUG ......(((((((...(((((.(((..((((...(((((...((((......))))...((((((((......).)))))))....)))))))))...))).)))))..))))))). (-28.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 640 78 31 124 78 aact:AA01445|5end_hypothetical_protein_969899..970129_POSITIVE 303 65 26 194 153 aact:AA01056|5end_conserved_hypothetical_protein_733867..734097_POSITIVE 290 93 55 188 139 aact:AA00416|5end_D-methionine_transport_system_permease_protein_(ABC_transport_system)_282677..282907_POSITIVE 289 74 35 206 175 aact:AA02590|5end_conserved_hypothetical_protein_1801113..1801343_POSITIVE 282 58 11 193 140 aact:AA00172|5end_conserved_hypothetical_protein_(possible_regulator_protein_/_nitrogen_fixation_protein)_121778..122008_POSITIVE 276 65 29 70 37 aact:AA02597|5end_ribonuclease_D_1807124..1807354_POSITIVE 268 79 37 148 106 aact:AA01566|5end_conserved_hypothetical_protein_1051811..1052041_POSITIVE 268 79 37 148 106 aact:AA01261|5end_conserved_hypothetical_protein_856443..856673_POSITIVE 267 70 29 169 118 aact:AA00248|5end_hypothetical_protein_173868..174098_POSITIVE 265 65 38 38 8 aact:AA00471|5end_3,4-dihydroxy-2-butanone_4-phosphate_synthase;_GTP_cyclohydrase_II_328262..328492_POSITIVE 264 58 18 133 83 aact:AA00722|5end_nucleoside_diphosphate_kinase_496020..496250_POSITIVE 257 60 11 143 95 aact:AA01838|5end_conserved_hypothetical_protein_1236396..1236626_POSITIVE 257 69 25 45 2 aact:AA01143|5end_hypothetical_protein_783111..783341_POSITIVE 257 65 26 144 94 aact:AA00866|5end_conserved_hypothetical_protein_599925..600155_POSITIVE 255 57 14 85 48 aact:AA00365|5end_30S_ribosomal_protein_S19_245914..246144_POSITIVE 252 77 32 177 134 aact:AA01294|5end_DNA_adenine_methylase_876415..876645_POSITIVE 250 70 22 139 95 aact:AA02153|5end_iron(III)_ABC_transporter,_permease_protein_1472530..1472760_POSITIVE 249 68 28 91 52 aact:AA01194|5end_acyl_carrier_protein_819595..819825_POSITIVE 248 66 27 168 135 aact:AA01138|5end_anaerobic_dimethyl_sulfoxide_reductase,_chain_B_780991..781221_POSITIVE 247 68 28 45 10 aact:AA01510|5end_ABC_transporter,_ATP-binding_protein/permease_1016430..1016660_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 806 100 54 60 14 aact:AA00227|3end_hypothetical_sequence_156642..156792_POSITIVE 278 110 63 44 5 aact:AA01857|3end_glutamate-ammonia-ligase_adenylyltransferase_1250357..1250507_POSITIVE 257 60 11 63 15 aact:AA01837|3end_transcriptional_regulatory_protein_1236396..1236546_POSITIVE 255 67 22 100 55 aact:AA01782|3end_molybdenum_transport_protein;_transcriptional_regulator_1203735..1203885_POSITIVE 255 57 14 71 34 aact:AA00363|3end_50S_ribosomal_protein_L22_245980..246130_POSITIVE 254 77 37 62 25 aact:AA01911|3end_hypothetical_protein_1288284..1288434_POSITIVE 254 67 22 115 62 aact:AA00988|3end_conserved_hypothetical_protein_692264..692414_POSITIVE 252 77 32 65 22 aact:AA01292|3end_single-stranded_DNA_binding_protein_876383..876533_POSITIVE 250 70 22 139 95 aact:AA02152|3end_ferric_enterobactin_transport;_ABC_transporter_ATP-binding_protein_1472610..1472760_POSITIVE 249 68 28 88 49 aact:AA01193|3end_AMP-binding_enzyme_819672..819822_POSITIVE 247 68 28 49 14 aact:AA01509|3end_ABC_system_transport_protein,_ATP_binding/permease_1016514..1016664_POSITIVE 245 65 36 96 68 aact:AA02674|3end_formate_dehydrogenase-related_protein_1860732..1860882_POSITIVE 245 68 38 85 52 aact:AA00511|3end_nicotinamide_mononucleotide_transporter_354482..354632_POSITIVE 242 67 26 40 4 aact:AA01018|3end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_712300..712450_POSITIVE 241 70 36 143 110 aact:AA01943|3end_conserved_hypothetical_protein_1313088..1313238_POSITIVE 240 45 3 81 34 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE 240 45 3 81 34 aact:AA01261|3end_conserved_hypothetical_protein_856723..856873_POSITIVE 238 67 27 64 18 aact:AA02427|3end_hypothetical_protein_1674396..1674546_POSITIVE 237 98 53 129 80 aact:AA01635|3end_ribonucleoside_diphosphate_reductase,_beta_chain_1105822..1105972_POSITIVE 234 75 40 114 86 aact:AA01637|3end_ribonucleoside_diphosphate_reductase_1109004..1109154_POSITIVE