Origin IGS: taatttacaaactaaaaaaggcttgggaattccaagcctttttcgttacatagctacataaaatgcggccataaaaaaatgcgttttaacgccgaattgttatttcaattcgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacgaattgaaataacaattcggcgttaaaacgcatttttttatggccgcattttatgtagctatgtaacgaaaaaggcttggaattcccaagccttttttagtttgtaaatta Mask Tandem Repeat Region ================================================ taatttacaaactaaaaaaggcttgggaattccaagcctttttcgttacatagctacataaaatgcggccataaaaaaatgcgttttaacgccgaattgttatttcaattcgtta Find is-nt database================================================ Query_seq: AA01368:AA01369|AA01368:AA01369:50S ribosomal protein L19:rod shape-determining protein:->->:924973..925087 115 taatttacaaactaaaaaaggcttgggaattccaagcctttttcgttacatagctacataaaatgcggccataaaaaaatgcgttttaacgccgaattgttatttcaattcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01368:AA01369|AA01368:AA01369:50S ribosomal protein L19:rod shape-determining protein:->->:924973..925087 115 taatttacaaactaaaaaaggcttgggaattccaagcctttttcgttacatagctacataaaatgcggccataaaaaaatgcgttttaacgccgaattgttatttcaattcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01368:AA01369|AA01368:AA01369:50S ribosomal protein L19:rod shape-determining protein:->->:924973..925087 115 taatttacaaactaaaaaaggcttgggaattccaagcctttttcgttacatagctacataaaatgcggccataaaaaaatgcgttttaacgccgaattgttatttcaattcgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatttacaaactaaaaaaggcttgggaattccaagcctttttcgttacatagctacataaaatgcggccataaaaaaatgcgttttaacgccgaattgttatttcaattcgtta Predict ORF larger than 30AA ================================================ taatttacaaactaaaaaaggcttgggaattccaagcctttttcgttacatagctacataaaatgcggccataaaaaaatgcgttttaacgccgaattgttatttcaattcgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatttacaaactaaaaaaggcttgggaattccaagcctttttcgttacatagctacataaaatgcggccataaaaaaatgcgttttaacgccgaattgttatttcaattcgtta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -10.3 Tail_Score: -5.42514 Start: 20 End: 38 Full_Region: ctatgtaacgaaaaa ggcttgg aattc ccaagcc ttttttagtttgtaa ...................ggcttggaattcccaagcc............................................................................. Find igs database================================================ Query_seq: AA01368:AA01369|AA01368:AA01369:50S ribosomal protein L19:rod shape-determining protein:->->:924973..925087 115 taatttacaaactaaaaaaggcttgggaattccaagcctttttcgttacatagctacataaaatgcggccataaaaaaatgcgttttaacgccgaattgttatttcaattcgtta Intra-Species Hit: Count: 1 Min: 1 Max: 115 Len: 115 Subject: aact_AA01368_AA01369|50S ribosomal protein L19:rod shape-determining protein|POSITIVE:POSITIVE|[924973,925087]|115 HSP 1 e-value: 1.0E-46 bit: 186.0 Len: 115 Query Start:1 Query End:115 Subject Strand: POSITIVE Subject Start: 1 Subject End: 115 taatttacaaactaaaaaaggcttgggaattccaagcctttttcgttacatagctacataaaatgcggccatnnnnnnntgcgttttaacgccgaattgttatttcaattcgtta taatttacaaactaaaaaaggcttgggaattccaagcctttttcgttacatagctacataaaatgcggccataaaaaaatgcgttttaacgccgaattgttatttcaattcgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUACAAACUAAAAAAGGCUUGGGAAUUCCAAGCCUUUUUCGUUACAUAGCUACAUAAAAUGCGGCCAUAAAAAAAUGCGUUUUAACGCCGAAUUGUUAUUUCAAUUCGUUA .........(((..((((((((((((.....)))))))))))).)))...........(((((((((............)))))))))....(((((((......)))))))... (-29.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACGAAUUGAAAUAACAAUUCGGCGUUAAAACGCAUUUUUUUAUGGCCGCAUUUUAUGUAGCUAUGUAACGAAAAAGGCUUGGAAUUCCCAAGCCUUUUUUAGUUUGUAAAUUA ...(((((((......)))))))((((....)))).......((((((..(((...)))..))))))....(((((((((((((.....)))))))))))))............. (-36.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 314 56 18 148 113 aact:AA00325|5end_conserved_hypothetical_protein_221314..221544_POSITIVE 306 69 21 149 97 aact:AA01710|5end_ribosomal_protein_S6_modification_protein_1159348..1159578_POSITIVE 303 40 17 96 75 aact:AA00109|5end_conserved_hypothetical_protein_78582..78812_POSITIVE 293 95 54 46 4 aact:AA01565|5end_conserved_hypothetical_protein_1050711..1050941_POSITIVE 290 49 4 192 138 aact:AA02215|5end_conserved_hypothetical_protein_1508800..1509030_POSITIVE 277 54 16 147 109 aact:AA00650|5end_15_kDa_peptidoglycan-associated_protein_444498..444728_POSITIVE 265 69 21 211 155 aact:AA02030|5end_threonyl-tRNA_synthetase_1382171..1382401_POSITIVE 263 94 56 41 2 aact:AA01798|5end_hypothetical_protein_1212070..1212300_POSITIVE 262 70 21 158 115 aact:AA01498|5end_conserved_hypothetical_protein_1007957..1008187_POSITIVE 261 68 21 140 96 aact:AA02526|5end_conserved_hypothetical_protein_1755225..1755455_POSITIVE 259 46 9 135 93 aact:AA02844|5end_Holliday_junction_DNA_helicase_1996942..1997172_POSITIVE 259 38 18 144 119 aact:AA01522|5end_conserved_hypothetical_protein_1022840..1023070_POSITIVE 253 73 34 223 170 aact:AA01996|5end_prephenate_dehydrogenase_1355314..1355544_POSITIVE 253 100 51 81 30 aact:AA00582|5end_conserved_hypothetical_protein_397904..398134_POSITIVE 250 55 13 153 109 aact:AA02333|5end_conserved_hypothetical_protein_1594817..1595047_POSITIVE 246 69 21 121 72 aact:AA00587|5end_hypothetical_protein_399975..400205_POSITIVE 243 104 66 210 177 aact:AA01247|5end_hypothetical_protein_849664..849894_POSITIVE 242 70 28 224 181 aact:AA02980|5end_conserved_hypothetical_protein_2083305..2083535_POSITIVE 240 106 64 121 78 aact:AA00449|5end_conserved_hypothetical_protein_312996..313226_POSITIVE 238 48 9 155 115 aact:AA01533|5end_conserved_hypothetical_protein_1028931..1029161_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 363 43 15 76 48 aact:AA01368|3end_50S_ribosomal_protein_L19_924912..925062_POSITIVE 314 56 18 75 40 aact:AA00324|3end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_221321..221471_POSITIVE 306 69 21 55 3 aact:AA01709|3end_glutathione-S-transferase-nitroreductase_A_fusion_protein;_modulator_of_drug_activity_A_1159334..1159484_POSITIVE 303 40 17 82 61 aact:AA00108|3end_conserved_hypothetical_protein_78648..78798_POSITIVE 297 69 21 144 92 aact:AA02540|3end_5-methyltetrahydropteroyltriglutamate-homocystein_e_methyltransferase_1769499..1769649_POSITIVE 290 49 4 82 28 aact:AA02214|3end_pyruvate_kinase_II_1508770..1508920_POSITIVE 285 94 52 59 15 aact:AA01796|3end_branched-chain_amino_acid_aminotransferase_1212168..1212318_POSITIVE 263 68 22 104 61 aact:AA01911|3end_hypothetical_protein_1288284..1288434_POSITIVE 262 70 21 98 55 aact:AA01497|3end_MioC_protein_1007977..1008127_POSITIVE 261 68 21 137 93 aact:AA02525|3end_conserved_hypothetical_protein_1755302..1755452_POSITIVE 259 46 9 75 33 aact:AA02842|3end_conserved_hypothetical_protein_1996962..1997112_POSITIVE 259 38 18 144 119 aact:AA01519.1|3end_conserved_hypothetical_protein_1022920..1023070_POSITIVE 259 38 18 111 86 aact:AA01520|3end_hypothetical_protein_1022887..1023037_POSITIVE 255 69 21 143 85 aact:AA01262|3end_hydrolase_860087..860237_POSITIVE 254 54 20 128 98 aact:AA00227|3end_hypothetical_sequence_156642..156792_POSITIVE 253 73 34 80 27 aact:AA01994|3end_chorismate_mutase;_prephenate_dehydrogenase_1355251..1355401_POSITIVE 253 100 51 63 12 aact:AA00581|3end_thiosulfate_sulfurtransferase_(rhodanese)_397966..398116_POSITIVE 246 69 21 92 43 aact:AA00586|3end_glycerol-3-phosphate_regulon_repressor_400026..400176_POSITIVE 243 104 66 147 114 aact:AA01245|3end_tyrosine-specific_transport_protein_2_849681..849831_POSITIVE 242 70 28 147 104 aact:AA02978|3end_sufI_protein_precursor_homolog_2083308..2083458_POSITIVE