Origin IGS: taattgaaccaaaattcaagttttcagcccgaatgcgtattggtgtttttacctttagaacaggaaatttcacaatattaacaatccggctttggtttcggtgaatatcgttatttgcgttattacgaaggcaaagtgggagtggctaaaatgttgtta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taacaacattttagccactcccactttgccttcgtaataacgcaaataacgatattcaccgaaaccaaagccggattgttaatattgtgaaatttcctgttctaaaggtaaaaacaccaatacgcattcgggctgaaaacttgaattttggttcaatta Mask Tandem Repeat Region ================================================ taattgaaccaaaattcaagttttcagcccgaatgcgtattggtgtttttacctttagaacaggaaatttcacaatattaacaatccggctttggtttcggtgaatatcgttatttgcgttattacgaaggcaaagtgggagtggctaaaatgttgtta Find is-nt database================================================ Query_seq: AA01360:AA01363|AA01360:AA01363:XAA-pro aminopeptidase (X-pro aminopeptidase):hypothetical protein:->->:922486..922644 159 taattgaaccaaaattcaagttttcagcccgaatgcgtattggtgtttttacctttagaacaggaaatttcacaatattaacaatccggctttggtttcggtgaatatcgttatttgcgttattacgaaggcaaagtgggagtggctaaaatgttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01360:AA01363|AA01360:AA01363:XAA-pro aminopeptidase (X-pro aminopeptidase):hypothetical protein:->->:922486..922644 159 taattgaaccaaaattcaagttttcagcccgaatgcgtattggtgtttttacctttagaacaggaaatttcacaatattaacaatccggctttggtttcggtgaatatcgttatttgcgttattacgaaggcaaagtgggagtggctaaaatgttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01360:AA01363|AA01360:AA01363:XAA-pro aminopeptidase (X-pro aminopeptidase):hypothetical protein:->->:922486..922644 159 taattgaaccaaaattcaagttttcagcccgaatgcgtattggtgtttttacctttagaacaggaaatttcacaatattaacaatccggctttggtttcggtgaatatcgttatttgcgttattacgaaggcaaagtgggagtggctaaaatgttgtta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattgaaccaaaattcaagttttcagcccgaatgcgtattggtgtttttacctttagaacaggaaatttcacaatattaacaatccggctttggtttcggtgaatatcgttatttgcgttattacgaaggcaaagtgggagtggctaaaatgttgtta Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 3 End: 101 .. M E P K F K F S A R M R I G V F T F R T G N F T I L T I R L W F R .......................................................... Protein_Len: 35 Strand: - Start: 2 End: 106 . I S G F N L N E A R I R I P T K V K L V P F K V I N V I R S Q N R H M ..................................................... taattgaaccaaaattcaagttttcagcccgaatgcgtattggtgtttttacctttagaacaggaaatttcacaatattaacaatccggctttggtttcggtgaatatcgttatttgcgttattacgaaggcaaagtgggagtggctaaaatgttgtta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattgaaccaaaattcaagttttcagcccgaatgcgtattggtgtttttacctttagaacaggaaatttcacaatattaacaatccggctttggtttcggtgaatatcgttatttgcgttattacgaaggcaaagtgggagtggctaaaatgttgtta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01360:AA01363|AA01360:AA01363:XAA-pro aminopeptidase (X-pro aminopeptidase):hypothetical protein:->->:922486..922644 159 taattgaaccaaaattcaagttttcagcccgaatgcgtattggtgtttttacctttagaacaggaaatttcacaatattaacaatccggctttggtttcggtgaatatcgttatttgcgttattacgaaggcaaagtgggagtggctaaaatgttgtta Intra-Species Hit: Count: 1 Min: 1 Max: 159 Len: 159 Subject: aact_AA01360_AA01363|XAA-pro aminopeptidase (X-pro aminopeptidase):hypothetical protein|POSITIVE:POSITIVE|[922486,922644]|159 HSP 1 e-value: 2.0E-85 bit: 315.0 Len: 159 Query Start:1 Query End:159 Subject Strand: POSITIVE Subject Start: 1 Subject End: 159 taattgaaccaaaattcaagttttcagcccgaatgcgtattggtgtttttacctttagaacaggaaatttcacaatattaacaatccggctttggtttcggtgaatatcgttatttgcgttattacgaaggcaaagtgggagtggctaaaatgttgtta taattgaaccaaaattcaagttttcagcccgaatgcgtattggtgtttttacctttagaacaggaaatttcacaatattaacaatccggctttggtttcggtgaatatcgttatttgcgttattacgaaggcaaagtgggagtggctaaaatgttgtta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUGAACCAAAAUUCAAGUUUUCAGCCCGAAUGCGUAUUGGUGUUUUUACCUUUAGAACAGGAAAUUUCACAAUAUUAACAAUCCGGCUUUGGUUUCGGUGAAUAUCGUUAUUUGCGUUAUUACGAAGGCAAAGUGGGAGUGGCUAAAAUGUUGUUA .................((((((((.((......)).((..((((....))))..)).....)))))))).((((((((..((.(((.((((((.(((((...((((.(((.....))).)))).))))).)))))).))).)).....)))))))).. (-38.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAACAUUUUAGCCACUCCCACUUUGCCUUCGUAAUAACGCAAAUAACGAUAUUCACCGAAACCAAAGCCGGAUUGUUAAUAUUGUGAAAUUUCCUGUUCUAAAGGUAAAAACACCAAUACGCAUUCGGGCUGAAAACUUGAAUUUUGGUUCAAUUA ........((((((((..(((..(((((..((((.((((.((.......)).))))...))))..)))))..))).(((...(((((((......(((.......)))......)).))))).)))....))))))))..((((((....))))))... (-29.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 333 121 85 69 29 aact:AA01848|5end_argininosuccinate_synthetase_1242278..1242508_POSITIVE 308 140 93 179 120 aact:AA00514|5end_hypothetical_protein_355609..355839_POSITIVE 302 58 17 177 131 aact:AA01885|5end_hydrogenase_nickel_incorporation_protein_1271117..1271347_POSITIVE 301 124 87 200 170 aact:AA01810|5end_electron_transport_complex_protein_1219250..1219480_POSITIVE 295 148 107 227 189 aact:AA01918|5end_anaerobic_C4-dicarboxylate_transporter_1290931..1291161_POSITIVE 293 139 94 60 20 aact:AA02242|5end_molybdenum_cofactor_biosynthesis_protein_A_1523998..1524228_POSITIVE 290 119 76 224 190 aact:AA00299|5end_hypothetical_protein_204256..204486_POSITIVE 289 130 87 144 98 aact:AA01428|5end_acetolactate_synthase_958688..958918_POSITIVE 283 127 87 81 37 aact:AA00759|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_I_520603..520833_POSITIVE 280 45 3 218 177 aact:AA01345|5end_conserved_hypothetical_protein_(possible_membrane_protein)_912624..912854_POSITIVE 279 127 86 231 190 aact:AA02670|5end_hypothetical_protein_1858049..1858279_POSITIVE 279 146 109 34 5 aact:AA01675|5end_probable_multidrug_resistance_protein;_multidrug-efflux_transporter_1132115..1132345_POSITIVE 278 133 93 83 40 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 277 140 92 231 177 aact:AA02531|5end_conserved_hypothetical_protein_1758383..1758613_POSITIVE 277 140 91 66 22 aact:AA01865|5end_thiamin_ABC_transporter,_periplasmic-binding_protein_1252228..1252458_POSITIVE 276 138 91 93 38 aact:AA01982|5end_maltodextrin_phosphorylase_1339387..1339617_POSITIVE 276 129 85 64 7 aact:AA00401|5end_FKBP-type_peptidyl-prolyl_cis-trans_isomerase_slyD_(PPiase)_268361..268591_POSITIVE 276 140 95 223 179 aact:AA00310|5end_conserved_hypothetical_protein_210193..210423_POSITIVE 275 140 94 51 5 aact:AA02261|5end_30S_ribosomal_protein_S9_1538013..1538243_POSITIVE 275 120 80 127 85 aact:AA01066|5end_conserved_hypothetical_protein_738380..738610_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 293 139 94 44 4 aact:AA02241|3end_molybdenum_cofactor_biosynthesis_protein_C_1524062..1524212_POSITIVE 283 127 87 94 50 aact:AA00758|3end_hypothetical_protein_520696..520846_POSITIVE 279 146 109 34 5 aact:AA01674|3end_hypothetical_protein_1132195..1132345_POSITIVE 275 120 80 59 17 aact:AA01064|3end_conserved_hypothetical_protein_(possible_aminomethyltransferase)_738392..738542_POSITIVE 274 145 113 123 94 aact:AA02233|3end_asparaginyl-tRNA_synthetase_1521357..1521507_POSITIVE 272 140 93 88 45 aact:AA00521|3end_phosphatase_358324..358474_POSITIVE 271 44 18 86 56 aact:AA01861|3end_phosphomethylpyrimidine_kinase_1252107..1252257_POSITIVE 271 125 85 148 94 aact:AA01428|3end_acetolactate_synthase_959625..959775_POSITIVE 270 146 106 57 16 aact:AA01151|3end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_789422..789572_POSITIVE 270 139 99 82 36 aact:AA00162|3end_pilin_C_biogenesis_protein/protein_transport_protein_116086..116236_POSITIVE 267 145 104 136 86 aact:AA02682|3end_hydrogenase_maturation_protein_1868666..1868816_POSITIVE 266 64 28 139 94 aact:AA01209|3end_conserved_hypothetical_protein_827007..827157_POSITIVE 263 146 101 135 93 aact:AA01755|3end_cobalt_membrane_transport_protein_1186555..1186705_POSITIVE 263 146 101 122 82 aact:AA00047|3end_formate_dependent_nitrite_reductase,_cytochrome_c552_38353..38503_POSITIVE 261 146 101 70 15 aact:AA00865|3end_conserved_hypothetical_protein_600014..600164_POSITIVE 261 149 104 64 20 aact:AA00609|3end_outer_membrane_protein_416327..416477_POSITIVE 260 120 78 76 36 aact:AA01185|3end_conserved_hypothetical_protein_(possible_membrane_protein)_813401..813551_POSITIVE 259 128 82 149 103 aact:AA00120|3end_ATP-dependent_Clp_protease_ATP-binding_subunit_90281..90431_POSITIVE 258 115 84 46 8 aact:AA02108|3end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436643..1436793_POSITIVE 253 128 87 139 96 aact:AA01067|3end_30S_ribosomal_protein_S2_739697..739847_POSITIVE