Origin IGS: aggtattatttggtaggagaccccgaggtgccgttgcgggtggtcggtccttgaaccctacggttcaagggaattagttgaaatcatttttaggcttcttagtcgagctaagcctgcacaccaatgacaggaaaccaattcataagattaaaagtatcggctcagggacgtaacccactggcgaacacctcaggaaattcttttacttaatactactcgaaattacgtttaatgactagcacaaattctttttctcagaatacttttagttatctgaaatcacggtgaaattctca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgagaatttcaccgtgatttcagataactaaaagtattctgagaaaaagaatttgtgctagtcattaaacgtaatttcgagtagtattaagtaaaagaatttcctgaggtgttcgccagtgggttacgtccctgagccgatacttttaatcttatgaattggtttcctgtcattggtgtgcaggcttagctcgactaagaagcctaaaaatgatttcaactaattcccttgaaccgtagggttcaaggaccgaccacccgcaacggcacctcggggtctcctaccaaataatacct Mask Tandem Repeat Region ================================================ aggtattatttggtaggagaccccgaggtgccgttgcgggtggtcggtccttgaaccctacggttcaagggaattagttgaaatcatttttaggcttcttagtcgagctaagcctgcacaccaatgacaggaaaccaattcataagattaaaagtatcggctcagggacgtaacccactggcgaacacctcaggaaattcttttacttaatactactcgaaattacgtttaatgactagcacaaattctttttctcagaatacttttagttatctgaaatcacggtgaaattctca Find is-nt database================================================ Query_seq: AA01357:AA01358|AA01357:AA01358:5-formyltetrahydrofolate cyclo-ligase-family protein:conserved hypothetical protein:->->:919846..920141 296 aggtattatttggtaggagaccccgaggtgccgttgcgggtggtcggtccttgaaccctacggttcaagggaattagttgaaatcatttttaggcttcttagtcgagctaagcctgcacaccaatgacaggaaaccaattcataagattaaaagtatcggctcagggacgtaacccactggcgaacacctcaggaaattcttttacttaatactactcgaaattacgtttaatgactagcacaaattctttttctcagaatacttttagttatctgaaatcacggtgaaattctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01357:AA01358|AA01357:AA01358:5-formyltetrahydrofolate cyclo-ligase-family protein:conserved hypothetical protein:->->:919846..920141 296 aggtattatttggtaggagaccccgaggtgccgttgcgggtggtcggtccttgaaccctacggttcaagggaattagttgaaatcatttttaggcttcttagtcgagctaagcctgcacaccaatgacaggaaaccaattcataagattaaaagtatcggctcagggacgtaacccactggcgaacacctcaggaaattcttttacttaatactactcgaaattacgtttaatgactagcacaaattctttttctcagaatacttttagttatctgaaatcacggtgaaattctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01357:AA01358|AA01357:AA01358:5-formyltetrahydrofolate cyclo-ligase-family protein:conserved hypothetical protein:->->:919846..920141 296 aggtattatttggtaggagaccccgaggtgccgttgcgggtggtcggtccttgaaccctacggttcaagggaattagttgaaatcatttttaggcttcttagtcgagctaagcctgcacaccaatgacaggaaaccaattcataagattaaaagtatcggctcagggacgtaacccactggcgaacacctcaggaaattcttttacttaatactactcgaaattacgtttaatgactagcacaaattctttttctcagaatacttttagttatctgaaatcacggtgaaattctca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aggtattatttggtaggagaccccgaggtgccgttgcgggtggtcggtccttgaaccctacggttcaagggaattagttgaaatcatttttaggcttcttagtcgagctaagcctgcacaccaatgacaggaaaccaattcataagattaaaagtatcggctcagggacgtaacccactggcgaacacctcaggaaattcttttacttaatactactcgaaattacgtttaatgactagcacaaattctttttctcagaatacttttagttatctgaaatcacggtgaaattctca Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 78 End: 170 ............................................................................. M K S F L G F L V E L S L H T N D R K P I H K I K S I G S G T .............................................................................................................................. aggtattatttggtaggagaccccgaggtgccgttgcgggtggtcggtccttgaaccctacggttcaagggaattagttgaaatcatttttaggcttcttagtcgagctaagcctgcacaccaatgacaggaaaccaattcataagattaaaagtatcggctcagggacgtaacccactggcgaacacctcaggaaattcttttacttaatactactcgaaattacgtttaatgactagcacaaattctttttctcagaatacttttagttatctgaaatcacggtgaaattctca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aggtattatttggtaggagaccccgaggtgccgttgcgggtggtcggtccttgaaccctacggttcaagggaattagttgaaatcatttttaggcttcttagtcgagctaagcctgcacaccaatgacaggaaaccaattcataagattaaaagtatcggctcagggacgtaacccactggcgaacacctcaggaaattcttttacttaatactactcgaaattacgtttaatgactagcacaaattctttttctcagaatacttttagttatctgaaatcacggtgaaattctca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01357:AA01358|AA01357:AA01358:5-formyltetrahydrofolate cyclo-ligase-family protein:conserved hypothetical protein:->->:919846..920141 296 aggtattatttggtaggagaccccgaggtgccgttgcgggtggtcggtccttgaaccctacggttcaagggaattagttgaaatcatttttaggcttcttagtcgagctaagcctgcacaccaatgacaggaaaccaattcataagattaaaagtatcggctcagggacgtaacccactggcgaacacctcaggaaattcttttacttaatactactcgaaattacgtttaatgactagcacaaattctttttctcagaatacttttagttatctgaaatcacggtgaaattctca Intra-Species Hit: Count: 1 Min: 1 Max: 296 Len: 296 Subject: aact_AA01357_AA01358|5-formyltetrahydrofolate cyclo-ligase-family protein:conserved hypothetical protein|POSITIVE:POSITIVE|[919846,920141]|296 HSP 1 e-value: 1.0E-167 bit: 587.0 Len: 296 Query Start:1 Query End:296 Subject Strand: POSITIVE Subject Start: 1 Subject End: 296 aggtattatttggtaggagaccccgaggtgccgttgcgggtggtcggtccttgaaccctacggttcaagggaattagttgaaatcatttttaggcttcttagtcgagctaagcctgcacaccaatgacaggaaaccaattcataagattaaaagtatcggctcagggacgtaacccactggcgaacacctcaggaaattcttttacttaatactactcgaaattacgtttaatgactagcacaaattctttttctcagaatacttttagttatctgaaatcacggtgaaattctca aggtattatttggtaggagaccccgaggtgccgttgcgggtggtcggtccttgaaccctacggttcaagggaattagttgaaatcatttttaggcttcttagtcgagctaagcctgcacaccaatgacaggaaaccaattcataagattaaaagtatcggctcagggacgtaacccactggcgaacacctcaggaaattcttttacttaatactactcgaaattacgtttaatgactagcacaaattctttttctcagaatacttttagttatctgaaatcacggtgaaattctca Inter-species Hit: Count: 1 Min: 47 Max: 215 Len: 169 Subject: NC_002663_PM1721_PM1722|hypothetical protein:hypothetical protein|NEGATIVE:NEGATIVE|[1932013,1932291]|279 HSP 1 e-value: 3.0E-34 bit: 147.0 Len: 169 Query Start:47 Query End:215 Subject Strand: POSITIVE Subject Start: 48 Subject End: 213 ..............................................gtccttgaaccctacggttcaagggaattagttgaaatcatttttaggcttcttagtcgagctaagcctgcacaccaatgacaggaaaccaattcataagattaaaagtatcggctcagggacgtaacccactggcgaacacctcaggaaattcttttacttaatacta................................................................................. ..............................................gtccttgaacccaacggttcaagggaatcagttataactatttttaggcttcttagtcaagctaagcatgcacaccaacaattagaaaccgattcataa-aataaaagtatcggctcagggacataacccactggcaaacacctcaggtaat--ctttacttaatacta................................................................................. Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUCCUUGAACCCUACGGUUCAAGGGAAUUAGUUGAAAUCAUUUUUAGGCUUCUUAGUCGAGCUAAGCCUGCACACCAAUGACAGGAAACCAAUUCAUAAGAUUAAAAGUAUCGGCUCAGGGACGUAACCCACUGGCGAACACCUCAGGAAAUUCUUUUACUUAAUACUA .((((((((((....)))))))))).(((((.(((((..(((((((((.(((((((..((((....((((((......)).))))..............(((.......))).)))).(((......))).)))).)))..)))..))))))..))))).))))).... (-39.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUAUUAAGUAAAAGAAUUUCCUGAGGUGUUCGCCAGUGGGUUACGUCCCUGAGCCGAUACUUUUAAUCUUAUGAAUUGGUUUCCUGUCAUUGGUGUGCAGGCUUAGCUCGACUAAGAAGCCUAAAAAUGAUUUCAACUAAUUCCCUUGAACCGUAGGGUUCAAGGAC .............((((.((....((((((((.(((((.(((.....)))))).)).)))))))).))))))..(((((((((....((((((..((.((...(((((.....)))))..)).))..))))))...)))))))))(((((((((....))))))))).. (-49.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 341 148 108 198 155 aact:AA01429|5end_conserved_hypothetical_protein_959581..959811_POSITIVE 328 150 112 43 1 aact:AA02867|5end_ribonuclease_T_2013118..2013348_POSITIVE 328 146 106 107 62 aact:AA00602|5end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_409077..409307_POSITIVE 320 139 112 55 19 aact:AA00383|5end_chloride_channel_protein_258894..259124_POSITIVE 314 143 109 82 44 aact:AA01846|5end_conserved_hypothetical_protein_1241843..1242073_POSITIVE 307 145 107 55 6 aact:AA00475|5end_conserved_hypothetical_protein_(possible_N6-adenine-specific_methylase)_331621..331851_POSITIVE 305 148 113 41 4 aact:AA02895|5end_oligopeptide_transport_system_permease_protein,_C-terminal_2027484..2027714_POSITIVE 304 90 46 227 185 aact:AA01013|5end_phosphomannomutase_708029..708259_POSITIVE 302 90 48 203 150 aact:AA02999|5end_conserved_hypothetical_protein_2094275..2094505_POSITIVE 300 146 110 178 137 aact:AA02972|5end_conserved_hypothetical_protein_(tyrosine_phenol_lyase-related_protein)_2077381..2077611_POSITIVE 295 150 107 201 160 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 291 150 108 162 117 aact:AA02524|5end_orotidine_5'-phosphate_decarboxylase_1753313..1753543_POSITIVE 291 148 112 168 133 aact:AA00991|5end_conserved_hypothetical_protein_693191..693421_POSITIVE 288 80 47 157 118 aact:AA01755|5end_cobalt_membrane_transport_protein_1185825..1186055_POSITIVE 287 148 108 119 71 aact:AA00550|5end_ferredoxin-type_protein_376444..376674_POSITIVE 285 137 99 201 158 aact:AA00164|5end_dephospho-CoA_kinase_(dephosphocoenzyme_A_kinase)_116745..116975_POSITIVE 285 144 110 36 9 aact:AA00106|5end_1-hydroxy-2-methyl-2-(E)-butenyl_4-diphosphate_synthase_75544..75774_POSITIVE 285 150 106 88 42 aact:AA00050|5end_cytochrome_c-type_biogenesis_protein_precursor_(reductase)_39919..40149_POSITIVE 284 149 112 39 1 aact:AA01872|5end_conserved_hypothetical_protein_1258804..1259034_POSITIVE 284 137 110 105 70 aact:AA01066|5end_conserved_hypothetical_protein_738380..738610_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 328 146 106 69 24 aact:AA00601|3end_hypothetical_protein_409119..409269_POSITIVE 311 160 113 78 34 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 307 145 107 78 29 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 300 146 110 108 67 aact:AA02970|3end_tyrosine_phenol-lyase_2077391..2077541_POSITIVE 287 148 108 116 68 aact:AA00549|3end_ferredoxin-type_protein_376521..376671_POSITIVE 286 148 126 55 32 aact:AA02480|3end_excinuclease_ABC_subunit_B_1718442..1718592_POSITIVE 285 137 99 148 105 aact:AA00163|3end_type_IV_prepilin_peptidase;_type_II_secretion_pathway_related_protein_116772..116922_POSITIVE 284 137 110 37 2 aact:AA01064|3end_conserved_hypothetical_protein_(possible_aminomethyltransferase)_738392..738542_POSITIVE 279 150 110 82 36 aact:AA01156|3end_chaperonin_heat_shock_protein_33_homolog_792408..792558_POSITIVE 279 148 109 134 102 aact:AA00420|3end_tRNA/rRNA_methyltransferase_285506..285656_POSITIVE 278 143 101 81 36 aact:AA02543|3end_conserved_hypothetical_protein_1773434..1773584_POSITIVE 277 136 108 150 119 aact:AA01428|3end_acetolactate_synthase_959625..959775_POSITIVE 274 70 30 57 13 aact:AA02837|3end_conserved_hypothetical_protein_1992977..1993127_POSITIVE 271 157 111 96 33 aact:AA00933|3end_ribose_ABC_transporter,_ATP-binding_protein_645197..645347_POSITIVE 268 77 47 149 115 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 266 148 108 151 108 aact:AA02523|3end_conserved_hypothetical_protein_1753384..1753534_POSITIVE 264 149 113 40 6 aact:AA00740|3end_citrate_lyase_related_biosynthesis_protein_(2-(5'-triphosphoribosyl)-3'-dephosphocoenzyme-A_synthase)_506749..506899_POSITIVE 259 159 111 56 6 aact:AA01646|3end_hypothetical_protein_1115262..1115412_POSITIVE 255 154 114 89 34 aact:AA01524|3end_amino_acid_ABC_transporter,_ATP-binding_protein_1024344..1024494_POSITIVE 253 155 111 72 16 aact:AA00109|3end_conserved_hypothetical_protein_79153..79303_POSITIVE