Origin IGS: taatgtggagataaaaagtgcggtcgaaattcgcgatggattttgaaattgccgtgggtaaatttacccacggttacgcttggttcagcccctttggggctgttttcaattttctaagggagaatgattacttaatccagactaactcatcacttggatcaaaatgaggatttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaatcctcattttgatccaagtgatgagttagtctggattaagtaatcattctcccttagaaaattgaaaacagccccaaaggggctgaaccaagcgtaaccgtgggtaaatttacccacggcaatttcaaaatccatcgcgaatttcgaccgcactttttatctccacatta Mask Tandem Repeat Region ================================================ taatgtggagataaaaagtgcggtcgaaattcgcgatggattttgaaattgccgtgggtaaatttacccacggttacgcttggttcagcccctttggggctgttttcaattttctaagggagaatgattacttaatccagactaactcatcacttggatcaaaatgaggatttta Find is-nt database================================================ Query_seq: AA01351:AA01354|AA01351:AA01354:probable sulfatase:conserved hypothetical protein:->->:916454..916628 175 taatgtggagataaaaagtgcggtcgaaattcgcgatggattttgaaattgccgtgggtaaatttacccacggttacgcttggttcagcccctttggggctgttttcaattttctaagggagaatgattacttaatccagactaactcatcacttggatcaaaatgaggatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01351:AA01354|AA01351:AA01354:probable sulfatase:conserved hypothetical protein:->->:916454..916628 175 taatgtggagataaaaagtgcggtcgaaattcgcgatggattttgaaattgccgtgggtaaatttacccacggttacgcttggttcagcccctttggggctgttttcaattttctaagggagaatgattacttaatccagactaactcatcacttggatcaaaatgaggatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01351:AA01354|AA01351:AA01354:probable sulfatase:conserved hypothetical protein:->->:916454..916628 175 taatgtggagataaaaagtgcggtcgaaattcgcgatggattttgaaattgccgtgggtaaatttacccacggttacgcttggttcagcccctttggggctgttttcaattttctaagggagaatgattacttaatccagactaactcatcacttggatcaaaatgaggatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatgtggagataaaaagtgcggtcgaaattcgcgatggattttgaaattgccgtgggtaaatttacccacggttacgcttggttcagcccctttggggctgttttcaattttctaagggagaatgattacttaatccagactaactcatcacttggatcaaaatgaggatttta Predict ORF larger than 30AA ================================================ Protein_Len: 49 Strand: + Start: 18 End: 164 ................. M R S K F A M D F E I A V G K F T H G Y A W F S P F G A V F N F L R E N D Y L I Q T N S S L G S K ........... Protein_Len: 36 Strand: - Start: 3 End: 110 .. H P S L F H P R F E R H I K F N G H T F K G V T V S P E A G K P S N E M ................................................................. taatgtggagataaaaagtgcggtcgaaattcgcgatggattttgaaattgccgtgggtaaatttacccacggttacgcttggttcagcccctttggggctgttttcaattttctaagggagaatgattacttaatccagactaactcatcacttggatcaaaatgaggatttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatgtggagataaaaagtgcggtcgaaattcgcgatggattttgaaattgccgtgggtaaatttacccacggttacgcttggttcagcccctttggggctgttttcaattttctaagggagaatgattacttaatccagactaactcatcacttggatcaaaatgaggatttta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 73 HP_score: -8.4 Tail_Score: -3.57792 Start: 49 End: 76 Full_Region: gggctgaaccaagcg taaccgtgggta aatt tacccacggcaa tttcaaaatccatcg ................................................taaccgtgggtaaatttacccacggcaa................................................................................................... TransTerm Strand: + Conf: 100 HP_score: -13.7 Tail_Score: -4.4595 Start: 86 End: 102 Full_Region: CGGTTACGCTTGGTT CAGCCCC TTT GGGGCTG TTTTCAATTTTCTAA .....................................................................................CAGCCCCTTTGGGGCTG......................................................................... Find igs database================================================ Query_seq: AA01351:AA01354|AA01351:AA01354:probable sulfatase:conserved hypothetical protein:->->:916454..916628 175 taatgtggagataaaaagtgcggtcgaaattcgcgatggattttgaaattgccgtgggtaaatttacccacggttacgcttggttcagcccctttggggctgttttcaattttctaagggagaatgattacttaatccagactaactcatcacttggatcaaaatgaggatttta Intra-Species Hit: Count: 1 Min: 1 Max: 175 Len: 175 Subject: aact_AA01351_AA01354|probable sulfatase:conserved hypothetical protein|POSITIVE:POSITIVE|[916454,916628]|175 HSP 1 e-value: 8.0E-95 bit: 347.0 Len: 175 Query Start:1 Query End:175 Subject Strand: POSITIVE Subject Start: 1 Subject End: 175 taatgtggagataaaaagtgcggtcgaaattcgcgatggattttgaaattgccgtgggtaaatttacccacggttacgcttggttcagcccctttggggctgttttcaattttctaagggagaatgattacttaatccagactaactcatcacttggatcaaaatgaggatttta taatgtggagataaaaagtgcggtcgaaattcgcgatggattttgaaattgccgtgggtaaatttacccacggttacgcttggttcagcccctttggggctgttttcaattttctaagggagaatgattacttaatccagactaactcatcacttggatcaaaatgaggatttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUGUGGAGAUAAAAAGUGCGGUCGAAAUUCGCGAUGGAUUUUGAAAUUGCCGUGGGUAAAUUUACCCACGGUUACGCUUGGUUCAGCCCCUUUGGGGCUGUUUUCAAUUUUCUAAGGGAGAAUGAUUACUUAAUCCAGACUAACUCAUCACUUGGAUCAAAAUGAGGAUUUUA ...(.((((..........(((.(((((((((.....)))))))))....((((((((((....))))))))))..)))......(((((((...)))))))...(((.(((((....))))).)))........)))).)....(((((.............)))))....... (-51.42) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUCCUCAUUUUGAUCCAAGUGAUGAGUUAGUCUGGAUUAAGUAAUCAUUCUCCCUUAGAAAAUUGAAAACAGCCCCAAAGGGGCUGAACCAAGCGUAACCGUGGGUAAAUUUACCCACGGCAAUUUCAAAAUCCAUCGCGAAUUUCGACCGCACUUUUUAUCUCCACAUUA .............((((.....((((((..((.(..(((.........(((((((.....))))...)))...(((((((...)))))))..)))..)....(((((((((....))))))))).........))..)))))).....))))....................... (-45.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 607 98 51 165 116 aact:AA00978|5end_conserved_hypothetical_protein_683399..683629_POSITIVE 427 100 51 140 81 aact:AA01079|5end_conserved_hypothetical_protein_747404..747634_POSITIVE 426 58 11 61 9 aact:AA00482|5end_hydrolase_(haloacid_dehalogenase-like)_(HAD_superfamily)_335232..335462_POSITIVE 419 46 5 132 95 aact:AA00126|5end_conserved_hypothetical_protein_91053..91283_POSITIVE 418 99 51 164 113 aact:AA02466|5end_N-acetylneuraminate_lyase_1704963..1705193_POSITIVE 401 45 7 143 94 aact:AA02288|5end_conserved_hypothetical_protein_1558590..1558820_POSITIVE 400 55 12 103 67 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 391 52 14 114 78 aact:AA02362|5end_conserved_hypothetical_protein_1619905..1620135_POSITIVE 384 60 15 88 42 aact:AA00866|5end_conserved_hypothetical_protein_599925..600155_POSITIVE 378 52 15 200 165 aact:AA02754|5end_conserved_hypothetical_protein_1928949..1929179_POSITIVE 376 130 81 197 143 aact:AA01848|5end_argininosuccinate_synthetase_1242278..1242508_POSITIVE 366 97 51 109 53 aact:AA01932|5end_tRNA_pseudouridine_synthase_B_1305098..1305328_POSITIVE 366 58 14 148 98 aact:AA00835|5end_UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapep_tide)_575212..575442_POSITIVE 356 52 13 180 142 aact:AA02722|5end_cytidine_deaminase_1905379..1905609_POSITIVE 354 55 14 207 171 aact:AA00541|5end_conserved_hypothetical_protein_370140..370370_POSITIVE 354 55 14 117 81 aact:AA00540|5end_hypothetical_protein_370050..370280_POSITIVE 354 109 77 166 128 aact:AA00054|5end_cytochrome_c_biogenesis_protein_41393..41623_POSITIVE 353 48 4 214 172 aact:AA00314|5end_conserved_hypothetical_protein_(possible_Kmt1_protein)_212999..213229_POSITIVE 349 58 15 88 36 aact:AA02239|5end_molybdopterin_biosynthesis_protein_D_1523216..1523446_POSITIVE 348 60 15 170 118 aact:AA00759|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_I_520603..520833_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 455 52 12 73 35 aact:AA02781|3end_protein-export_membrane_protein_1945981..1946131_POSITIVE 427 100 51 109 50 aact:AA01078|3end_transcriptional_regulator_(probable_MerR_family)_747453..747603_POSITIVE 409 93 51 104 56 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 400 55 12 88 52 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 391 52 14 76 40 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE 384 60 15 97 51 aact:AA00865|3end_conserved_hypothetical_protein_600014..600164_POSITIVE 378 52 15 39 4 aact:AA02751|3end_L-lactate_permease_1928868..1929018_POSITIVE 372 56 14 98 45 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 366 97 51 106 50 aact:AA01930|3end_ribosome-binding_factor_A_1305175..1305325_POSITIVE 366 58 14 74 24 aact:AA00833|3end_UDP-N-acetylmuramate-alanine_ligase_575218..575368_POSITIVE 361 73 33 39 2 aact:AA01351|3end_probable_sulfatase_916393..916543_POSITIVE 356 52 13 55 17 aact:AA02721|3end_spermidine/putrescine_ABC_transporter,_periplasmic-binding_protein_1905334..1905484_POSITIVE 353 48 4 57 15 aact:AA00312|3end_conserved_hypothetical_protein_(possible_hydroxypyruvate_isomerase)_212922..213072_POSITIVE 349 58 15 84 32 aact:AA02237|3end_molybdopterin_biosynthesis_protein_E_chain_1523292..1523442_POSITIVE 344 60 11 69 18 aact:AA00146|3end_UDP-glucose/GDP-mannose_dehydrogenase_105944..106094_POSITIVE 340 99 51 61 12 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 338 54 14 97 57 aact:AA02142|3end_outer_membrane_protein_OprM_precursor_1467532..1467682_POSITIVE 329 75 33 110 70 aact:AA02959|3end_pyrroline-5-carboxylate_(P5C)_reductase_2070976..2071126_POSITIVE 329 104 77 41 7 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 327 55 17 34 1 aact:AA00539|3end_ADP-L-glycero-D-manno-heptose-6-epimerase_370047..370197_POSITIVE