Origin IGS: taaccggaattgtttgtagaaccgaccgaacttttgagcaatcaaagtgcggtttttttatagcgtaaatcctgctcatgttatatttaaaagcatatagcattgcagtaaaattcattctcgaagacgaccgcacttttaggagtaggaaaaaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gttttttcctactcctaaaagtgcggtcgtcttcgagaatgaattttactgcaatgctatatgcttttaaatataacatgagcaggatttacgctataaaaaaaccgcactttgattgctcaaaagttcggtcggttctacaaacaattccggtta Mask Tandem Repeat Region ================================================ taaccggaattgtttgtagaaccgaccgaacttttgagcaatcaaagtgcggtttttttatagcgtaaatcctgctcatgttatatttaaaagcatatagcattgcagtaaaattcattctcgaagacgaccgcacttttaggagtaggaaaaaac Find is-nt database================================================ Query_seq: AA01341:AA01342|AA01341:AA01342:acriflavine resistance protein:conserved hypotheticl protein (possible ribosomal RNA adenine dimethylase):->->:910192..910347 156 taaccggaattgtttgtagaaccgaccgaacttttgagcaatcaaagtgcggtttttttatagcgtaaatcctgctcatgttatatttaaaagcatatagcattgcagtaaaattcattctcgaagacgaccgcacttttaggagtaggaaaaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01341:AA01342|AA01341:AA01342:acriflavine resistance protein:conserved hypotheticl protein (possible ribosomal RNA adenine dimethylase):->->:910192..910347 156 taaccggaattgtttgtagaaccgaccgaacttttgagcaatcaaagtgcggtttttttatagcgtaaatcctgctcatgttatatttaaaagcatatagcattgcagtaaaattcattctcgaagacgaccgcacttttaggagtaggaaaaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01341:AA01342|AA01341:AA01342:acriflavine resistance protein:conserved hypotheticl protein (possible ribosomal RNA adenine dimethylase):->->:910192..910347 156 taaccggaattgtttgtagaaccgaccgaacttttgagcaatcaaagtgcggtttttttatagcgtaaatcctgctcatgttatatttaaaagcatatagcattgcagtaaaattcattctcgaagacgaccgcacttttaggagtaggaaaaaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaccggaattgtttgtagaaccgaccgaacttttgagcaatcaaagtgcggtttttttatagcgtaaatcctgctcatgttatatttaaaagcatatagcattgcagtaaaattcattctcgaagacgaccgcacttttaggagtaggaaaaaac Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: - Start: 2 End: 136 . V P I T Q L V S R V K S C D F H P K K I A Y I R S M N Y K F A Y L M A T F N M R S S S R M .................... taaccggaattgtttgtagaaccgaccgaacttttgagcaatcaaagtgcggtttttttatagcgtaaatcctgctcatgttatatttaaaagcatatagcattgcagtaaaattcattctcgaagacgaccgcacttttaggagtaggaaaaaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaccggaattgtttgtagaaccgaccgaacttttgagcaatcaaagtgcggtttttttatagcgtaaatcctgctcatgttatatttaaaagcatatagcattgcagtaaaattcattctcgaagacgaccgcacttttaggagtaggaaaaaac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01341:AA01342|AA01341:AA01342:acriflavine resistance protein:conserved hypotheticl protein (possible ribosomal RNA adenine dimethylase):->->:910192..910347 156 taaccggaattgtttgtagaaccgaccgaacttttgagcaatcaaagtgcggtttttttatagcgtaaatcctgctcatgttatatttaaaagcatatagcattgcagtaaaattcattctcgaagacgaccgcacttttaggagtaggaaaaaac Intra-Species Hit: Count: 1 Min: 1 Max: 156 Len: 156 Subject: aact_AA01341_AA01342|acriflavine resistance protein:conserved hypotheticl protein (possible ribosomal RNA adenine dimethylase)|POSITIVE:POSITIVE|[910192,910347]|156 HSP 1 e-value: 5.0E-71 bit: 268.0 Len: 156 Query Start:1 Query End:156 Subject Strand: POSITIVE Subject Start: 1 Subject End: 156 taaccggaattgtttgtagaaccgaccgaacttttgagcaatcaaagtgcggnnnnnnnatagcgtaaatcctgctcatgttatatttaaaagcatatagcattgcagtaaaattcattctcgaagacgaccgcacttttaggagtaggaaaaaac taaccggaattgtttgtagaaccgaccgaacttttgagcaatcaaagtgcggtttttttatagcgtaaatcctgctcatgttatatttaaaagcatatagcattgcagtaaaattcattctcgaagacgaccgcacttttaggagtaggaaaaaac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACCGGAAUUGUUUGUAGAACCGACCGAACUUUUGAGCAAUCAAAGUGCGGUUUUUUUAUAGCGUAAAUCCUGCUCAUGUUAUAUUUAAAAGCAUAUAGCAUUGCAGUAAAAUUCAUUCUCGAAGACGACCGCACUUUUAGGAGUAGGAAAAAAC ...........(((.((((((..(((((.(((((.((....))))))).))))).))))))))).....((((((((.((((((((........)))))))).(((.((....(((......)))....)).))).......))))))))...... (-39.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUUUUUCCUACUCCUAAAAGUGCGGUCGUCUUCGAGAAUGAAUUUUACUGCAAUGCUAUAUGCUUUUAAAUAUAACAUGAGCAGGAUUUACGCUAUAAAAAAACCGCACUUUGAUUGCUCAAAAGUUCGGUCGGUUCUACAAACAAUUCCGGUUA .................((((((((((........((..((((((...((((.(((.(((((........))))).)))..))))))))))..))........))))))))))..............((((...(((.....)))....))))... (-32.99) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 457 59 11 110 55 aact:AA01986|5end_pyruvate_dehydrogenase_complex_E2_1348753..1348983_POSITIVE 443 66 21 166 113 aact:AA01436|5end_fructose-bisphosphate_aldolase_class_II_962981..963211_POSITIVE 388 61 23 155 111 aact:AA02937|5end_conserved_hypothetical_protein_2056379..2056609_POSITIVE 382 63 24 160 115 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 372 59 14 164 111 aact:AA00464|5end_conserved_hypothetical_protein_323912..324142_POSITIVE 363 70 23 160 110 aact:AA01936|5end_tyrosyl-tRNA_synthetase_1307043..1307273_POSITIVE 362 139 92 94 51 aact:AA01989|5end_hypothetical_protein_1352039..1352269_POSITIVE 360 139 91 61 13 aact:AA02091|5end_hypothetical_protein_1424441..1424671_POSITIVE 358 53 13 146 100 aact:AA00613|5end_lipid-A-disaccharide_synthase_418713..418943_POSITIVE 355 147 102 231 184 aact:AA00579|5end_probable_DNA_recombination_protein_395265..395495_POSITIVE 346 66 21 162 110 aact:AA02252|5end_conserved_hypothetical_protein_1533840..1534070_POSITIVE 345 58 24 206 174 aact:AA02876|5end_conserved_hypothetical_protein_2018293..2018523_POSITIVE 345 140 94 123 74 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 345 75 31 169 119 aact:AA00987|5end_tetracenomycin_polyketide_synthesis_O-methyltransferase_689745..689975_POSITIVE 343 59 18 173 122 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 342 64 21 118 81 aact:AA01641|5end_hypothetical_protein_1110819..1111049_POSITIVE 340 66 24 165 126 aact:AA01027|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_F_717676..717906_POSITIVE 338 146 104 162 114 aact:AA02084|5end_conserved_hypothetical_protein_1417843..1418073_POSITIVE 333 60 11 90 39 aact:AA02681|5end_hypothetical_protein_1865749..1865979_POSITIVE 330 66 22 202 151 aact:AA01883|5end_recycling_protein;_permease_1269821..1270051_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 457 59 11 78 23 aact:AA01985|3end_pyruvate_dehydrogenase,_E1_component_1348801..1348951_POSITIVE 443 66 21 126 73 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE 388 61 23 69 25 aact:AA02936|3end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2056373..2056523_POSITIVE 372 59 14 70 17 aact:AA00463|3end_class_B_acid_phosphatase_323898..324048_POSITIVE 360 139 91 89 41 aact:AA02090|3end_glucose-1-phosphate_adenylyltransferase_1424549..1424699_POSITIVE 358 53 13 58 12 aact:AA00612|3end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_418705..418855_POSITIVE 355 147 102 87 40 aact:AA00578|3end_DNA_topoisomerase_395201..395351_POSITIVE 352 66 21 77 26 aact:AA00974|3end_glucosamine-fructose-6-phosphate_681127..681277_POSITIVE 346 66 21 85 33 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE 345 75 31 85 35 aact:AA00985|3end_transcriptional_regulator_(LysR_family)_689741..689891_POSITIVE 343 59 18 68 17 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 342 64 21 58 21 aact:AA01640|3end_restriction_enzyme_type_I_helicase_subunit_1110839..1110989_POSITIVE 340 63 26 44 1 aact:AA01690|3end_penicillin-binding_protein_1C_1149142..1149292_POSITIVE 338 146 104 87 39 aact:AA02082|3end_glutaminyl-tRNA_synthetase_1417848..1417998_POSITIVE 333 60 11 72 21 aact:AA02679|3end_formate_dehydrogenase,_gamma_subunit_1865811..1865961_POSITIVE 330 66 22 66 15 aact:AA01881|3end_adenylate_kinase_1269765..1269915_POSITIVE 329 140 102 130 96 aact:AA01259|3end_protoporphyrinogen_oxidase_854943..855093_POSITIVE 323 66 23 78 29 aact:AA01852|3end_peptide_chain_release_factor_3_1246551..1246701_POSITIVE 323 60 17 85 38 aact:AA01517|3end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1021791..1021941_POSITIVE 322 53 24 65 38 aact:AA00223|3end_catalase_(hydroperoxidase;_iron_heme_axial_ligand)_155808..155958_POSITIVE