Origin IGS: gccgctatcaagccaatcgcagaagcaatccctgacaccgcatgagattaatattacgccattctaaaatggcacgtttgtgtgtaaggctgcgaattttacagaaaaaaaaagtgcaattcaagcagcaccatgtggctgcgaggttatctttttatgagcagagatattcatttcattgtcttaagatagtaagatagcgcctggcctaaggcacaatttcatgtatctct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agagatacatgaaattgtgccttaggccaggcgctatcttactatcttaagacaatgaaatgaatatctctgctcataaaaagataacctcgcagccacatggtgctgcttgaattgcactttttttttctgtaaaattcgcagccttacacacaaacgtgccattttagaatggcgtaatattaatctcatgcggtgtcagggattgcttctgcgattggcttgatagcggc Mask Tandem Repeat Region ================================================ gccgctatcaagccaatcgcagaagcaatccctgacaccgcatgagattaatattacgccattctaaaatggcacgtttgtgtgtaaggctgcgaattttacagaaaaaaaaagtgcaattcaagcagcaccatgtggctgcgaggttatctttttatgagcagagatattcatttcattgtcttaagatagtaagatagcgcctggcctaaggcacaatttcatgtatctct Find is-nt database================================================ Query_seq: AA01331:AA01332|AA01331:AA01332:50S ribosomal protein L31:galactosyltransferase II:->->:898782..899014 233 gccgctatcaagccaatcgcagaagcaatccctgacaccgcatgagattaatattacgccattctaaaatggcacgtttgtgtgtaaggctgcgaattttacagaaaaaaaaagtgcaattcaagcagcaccatgtggctgcgaggttatctttttatgagcagagatattcatttcattgtcttaagatagtaagatagcgcctggcctaaggcacaatttcatgtatctct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01331:AA01332|AA01331:AA01332:50S ribosomal protein L31:galactosyltransferase II:->->:898782..899014 233 gccgctatcaagccaatcgcagaagcaatccctgacaccgcatgagattaatattacgccattctaaaatggcacgtttgtgtgtaaggctgcgaattttacagaaaaaaaaagtgcaattcaagcagcaccatgtggctgcgaggttatctttttatgagcagagatattcatttcattgtcttaagatagtaagatagcgcctggcctaaggcacaatttcatgtatctct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01331:AA01332|AA01331:AA01332:50S ribosomal protein L31:galactosyltransferase II:->->:898782..899014 233 gccgctatcaagccaatcgcagaagcaatccctgacaccgcatgagattaatattacgccattctaaaatggcacgtttgtgtgtaaggctgcgaattttacagaaaaaaaaagtgcaattcaagcagcaccatgtggctgcgaggttatctttttatgagcagagatattcatttcattgtcttaagatagtaagatagcgcctggcctaaggcacaatttcatgtatctct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gccgctatcaagccaatcgcagaagcaatccctgacaccgcatgagattaatattacgccattctaaaatggcacgtttgtgtgtaaggctgcgaattttacagaaaaaaaaagtgcaattcaagcagcaccatgtggctgcgaggttatctttttatgagcagagatattcatttcattgtcttaagatagtaagatagcgcctggcctaaggcacaatttcatgtatctct Predict ORF larger than 30AA ================================================ Protein_Len: 52 Strand: + Start: 42 End: 197 ......................................... M R L I L R H S K M A R L C V R L R I L Q K K K V Q F K Q H H V A A R L S F Y E Q R Y S F H C L K I V R .................................... Protein_Len: 38 Strand: - Start: 11 End: 124 .......... A L R L L L G Q C R M L N I N R W E L I A R K H T L S R I K C F F F T C N M ............................................................................................................. gccgctatcaagccaatcgcagaagcaatccctgacaccgcatgagattaatattacgccattctaaaatggcacgtttgtgtgtaaggctgcgaattttacagaaaaaaaaagtgcaattcaagcagcaccatgtggctgcgaggttatctttttatgagcagagatattcatttcattgtcttaagatagtaagatagcgcctggcctaaggcacaatttcatgtatctct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 85 Start: 96 End: 125 ...............................................................................................CTTGAATTGCACTTTTTTTTTCTGTAAAAT............................................................................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gccgctatcaagccaatcgcagaagcaatccctgacaccgcatgagattaatattacgccattctaaaatggcacgtttgtgtgtaaggctgcgaattttacagaaaaaaaaagtgcaattcaagcagcaccatgtggctgcgaggttatctttttatgagcagagatattcatttcattgtcttaagatagtaagatagcgcctggcctaaggcacaatttcatgtatctct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01331:AA01332|AA01331:AA01332:50S ribosomal protein L31:galactosyltransferase II:->->:898782..899014 233 gccgctatcaagccaatcgcagaagcaatccctgacaccgcatgagattaatattacgccattctaaaatggcacgtttgtgtgtaaggctgcgaattttacagaaaaaaaaagtgcaattcaagcagcaccatgtggctgcgaggttatctttttatgagcagagatattcatttcattgtcttaagatagtaagatagcgcctggcctaaggcacaatttcatgtatctct Intra-Species Hit: Count: 1 Min: 1 Max: 233 Len: 233 Subject: aact_AA01331_AA01332|50S ribosomal protein L31:galactosyltransferase II|POSITIVE:POSITIVE|[898782,899014]|233 HSP 1 e-value: 1.0E-113 bit: 408.0 Len: 233 Query Start:1 Query End:233 Subject Strand: POSITIVE Subject Start: 1 Subject End: 233 gccgctatcaagccaatcgcagaagcaatccctgacaccgcatgagattaatattacgccattctaaaatggcacgtttgtgtgtaaggctgcgaattttacagnnnnnnnnngtgcaattcaagcagcaccatgtggctgcgaggttatctttttatgagcagagatattcatttcattgtcttaagatagtaagatagcgcctggcctaaggcacaatttcatgtatctct gccgctatcaagccaatcgcagaagcaatccctgacaccgcatgagattaatattacgccattctaaaatggcacgtttgtgtgtaaggctgcgaattttacagaaaaaaaaagtgcaattcaagcagcaccatgtggctgcgaggttatctttttatgagcagagatattcatttcattgtcttaagatagtaagatagcgcctggcctaaggcacaatttcatgtatctct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCCGCUAUCAAGCCAAUCGCAGAAGCAAUCCCUGACACCGCAUGAGAUUAAUAUUACGCCAUUCUAAAAUGGCACGUUUGUGUGUAAGGCUGCGAAUUUUACAGAAAAAAAAAGUGCAAUUCAAGCAGCACCAUGUGGCUGCGAGGUUAUCUUUUUAUGAGCAGAGAUAUUCAUUUCAUUGUCUUAAGAUAGUAAGAUAGCGCCUGGCCUAAGGCACAAUUUCAUGUAUCUCU ...((((((..(((..((((((...((.....))...(((((((.............((((((....))))))...............((((((((((.(((...........))).)))))..)))))..))))))))))))))))(((((((........)))))))........((((((....))))))..))))))((((......)))).................. (-59.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGAGAUACAUGAAAUUGUGCCUUAGGCCAGGCGCUAUCUUACUAUCUUAAGACAAUGAAAUGAAUAUCUCUGCUCAUAAAAAGAUAACCUCGCAGCCACAUGGUGCUGCUUGAAUUGCACUUUUUUUUUCUGUAAAAUUCGCAGCCUUACACACAAACGUGCCAUUUUAGAAUGGCGUAAUAUUAAUCUCAUGCGGUGUCAGGGAUUGCUUCUGCGAUUGGCUUGAUAGCGGC ((((((((((......((((((......))))))..(((((......))))).......)))..)))))))..................((((.(((.(((((.(((((..(((((..((...........))..))))))))))...............((((((....))))))............))))).)))((((((((((((....))))))..)))))).)))). (-58.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 371 46 1 86 16 aact:AA00550|5end_ferredoxin-type_protein_376444..376674_POSITIVE 366 46 3 96 55 aact:AA01515|5end_SlyX_family_protein_1020682..1020912_POSITIVE 345 42 3 170 121 aact:AA02135.1|5end_hypothetical_protein_1458812..1459042_POSITIVE 338 40 2 62 13 aact:AA02035|5end_translation_initiation_factor_IF-3_1386349..1386579_POSITIVE 332 49 1 99 36 aact:AA01748|5end_thiol:disulfide_interchange_protein_1183062..1183292_POSITIVE 331 49 1 198 156 aact:AA02939|5end_phosphoserine_phosphatase_2056881..2057111_POSITIVE 331 60 11 214 158 aact:AA01316|5end_hypothetical_protein_891262..891492_POSITIVE 326 208 161 151 101 aact:AA01722|5end_conserved_hypothetical_protein_1163434..1163664_POSITIVE 318 48 1 75 6 aact:AA02367|5end_conserved_hypothetical_protein_1623174..1623404_POSITIVE 315 41 1 230 183 aact:AA01510|5end_ABC_transporter,_ATP-binding_protein/permease_1016430..1016660_POSITIVE 314 42 1 87 44 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 312 41 1 223 174 aact:AA02415|5end_hypothetical_protein_1658443..1658673_POSITIVE 310 152 114 65 24 aact:AA00274|5end_50S_ribosomal_protein_L32_192449..192679_POSITIVE 309 70 25 63 14 aact:AA00983|5end_aldo-keto_reductase_687570..687800_POSITIVE 307 50 2 218 168 aact:AA02723|5end_hypothetical_protein_1906263..1906493_POSITIVE 306 35 4 64 32 aact:AA00870|5end_rough_colony_protein_C_601735..601965_POSITIVE 304 46 18 99 66 aact:AA02188|5end_hypothetical_protein_1492785..1493015_POSITIVE 299 214 176 181 143 aact:AA01370|5end_rod_shape-determining_protein_925436..925666_POSITIVE 297 47 1 198 144 aact:AA02195|5end_oxaloacetate_decarboxylase_alpha_chain_1494501..1494731_POSITIVE 296 60 11 225 177 aact:AA02006|5end_PEP--fructosephosphotransferase_system_repressor,_DeoR_family_1363930..1364160_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 371 46 1 83 13 aact:AA00549|3end_ferredoxin-type_protein_376521..376671_POSITIVE 345 42 3 145 96 aact:AA02134|3end_aminopeptidase_N_1458867..1459017_POSITIVE 332 49 1 87 24 aact:AA01747|3end_single-stranded-DNA-specific_exonuclease_1183130..1183280_POSITIVE 326 208 161 120 70 aact:AA01720|3end_conserved_hypothetical_protein_1163483..1163633_POSITIVE 324 147 102 120 66 aact:AA01858|3end_conserved_hypothetical_protein_1251537..1251687_POSITIVE 320 50 1 139 95 aact:AA01062|3end_ribonuclease_PH_(RNase_PH)_736499..736649_POSITIVE 319 44 1 76 14 aact:AA00980|3end_hypothetical_protein_685409..685559_POSITIVE 318 48 1 73 4 aact:AA02366|3end_hypothetical_protein_1623252..1623402_POSITIVE 314 60 14 52 3 aact:AA01646|3end_hypothetical_protein_1115262..1115412_POSITIVE 314 42 1 76 33 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 305 41 1 68 20 aact:AA01666|3end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1129621..1129771_POSITIVE 304 46 18 81 48 aact:AA02187|3end_hypothetical_protein_1492847..1492997_POSITIVE 296 104 67 133 89 aact:AA01386|3end_30S_ribosomal_protein_S1_936281..936431_POSITIVE 296 96 57 97 45 aact:AA01202|3end_integrase/recombinase_824207..824357_POSITIVE 296 47 1 143 97 aact:AA00426|3end_conserved_hypothetical_protein_288205..288355_POSITIVE 290 163 125 37 5 aact:AA01391|3end_glycerol-3-phosphate_acyltransferase_940402..940552_POSITIVE 288 46 1 48 7 aact:AA01842|3end_conserved_hypothetical_protein_1238467..1238617_POSITIVE 287 48 1 76 16 aact:AA02525|3end_conserved_hypothetical_protein_1755302..1755452_POSITIVE 287 149 104 73 21 aact:AA01056|3end_conserved_hypothetical_protein_734168..734318_POSITIVE 286 178 133 151 89 aact:AA01903|3end_hypothetical_protein_1284215..1284365_POSITIVE