Origin IGS: aatttctttcagccctttatttcattgattctgtcccgcttaattccgtttaattcaacttaagtcctaacctgttatcaatttctaatattaagtggttggttacattgcgct .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agcgcaatgtaaccaaccacttaatattagaaattgataacaggttaggacttaagttgaattaaacggaattaagcgggacagaatcaatgaaataaagggctgaaagaaatt Mask Tandem Repeat Region ================================================ aatttctttcagccctttatttcattgattctgtcccgcttaattccgtttaattcaacttaagtcctaacctgttatcaatttctaatattaagtggttggttacattgcgct Find is-nt database================================================ Query_seq: AA01320:AA01321|AA01320:AA01321:hypothetical protein:DNA repair protein:->->:893600..893713 114 aatttctttcagccctttatttcattgattctgtcccgcttaattccgtttaattcaacttaagtcctaacctgttatcaatttctaatattaagtggttggttacattgcgct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01320:AA01321|AA01320:AA01321:hypothetical protein:DNA repair protein:->->:893600..893713 114 aatttctttcagccctttatttcattgattctgtcccgcttaattccgtttaattcaacttaagtcctaacctgttatcaatttctaatattaagtggttggttacattgcgct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01320:AA01321|AA01320:AA01321:hypothetical protein:DNA repair protein:->->:893600..893713 114 aatttctttcagccctttatttcattgattctgtcccgcttaattccgtttaattcaacttaagtcctaacctgttatcaatttctaatattaagtggttggttacattgcgct Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatttctttcagccctttatttcattgattctgtcccgcttaattccgtttaattcaacttaagtcctaacctgttatcaatttctaatattaagtggttggttacattgcgct Predict ORF larger than 30AA ================================================ aatttctttcagccctttatttcattgattctgtcccgcttaattccgtttaattcaacttaagtcctaacctgttatcaatttctaatattaagtggttggttacattgcgct Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatttctttcagccctttatttcattgattctgtcccgcttaattccgtttaattcaacttaagtcctaacctgttatcaatttctaatattaagtggttggttacattgcgct Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01320:AA01321|AA01320:AA01321:hypothetical protein:DNA repair protein:->->:893600..893713 114 aatttctttcagccctttatttcattgattctgtcccgcttaattccgtttaattcaacttaagtcctaacctgttatcaatttctaatattaagtggttggttacattgcgct Intra-Species Hit: Count: 1 Min: 1 Max: 114 Len: 114 Subject: aact_AA01320_AA01321|hypothetical protein:DNA repair protein|POSITIVE:POSITIVE|[893600,893713]|114 HSP 1 e-value: 1.0E-58 bit: 226.0 Len: 114 Query Start:1 Query End:114 Subject Strand: POSITIVE Subject Start: 1 Subject End: 114 aatttctttcagccctttatttcattgattctgtcccgcttaattccgtttaattcaacttaagtcctaacctgttatcaatttctaatattaagtggttggttacattgcgct aatttctttcagccctttatttcattgattctgtcccgcttaattccgtttaattcaacttaagtcctaacctgttatcaatttctaatattaagtggttggttacattgcgct Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUUCUUUCAGCCCUUUAUUUCAUUGAUUCUGUCCCGCUUAAUUCCGUUUAAUUCAACUUAAGUCCUAACCUGUUAUCAAUUUCUAAUAUUAAGUGGUUGGUUACAUUGCGCU ........(((((((((((((..((((((.(.((...((((((....(((......)))))))))....))..)..))))))....))))..))).))))))............ (-12.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGCGCAAUGUAACCAACCACUUAAUAUUAGAAAUUGAUAACAGGUUAGGACUUAAGUUGAAUUAAACGGAAUUAAGCGGGACAGAAUCAAUGAAAUAAAGGGCUGAAAGAAAUU (((.(...........((.((((((................((((....))))..(((......)))...)))))).))..((.......)).......).))).......... (-12.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 424 59 12 100 53 aact:AA02147|5end_hypothetical_protein_1469537..1469767_POSITIVE 398 110 61 160 112 aact:AA01762|5end_hypothetical_protein_1190386..1190616_POSITIVE 317 67 29 197 162 aact:AA01721|5end_hypothetical_protein_1163312..1163542_POSITIVE 317 67 29 163 128 aact:AA01720|5end_conserved_hypothetical_protein_1163278..1163508_POSITIVE 257 50 8 139 98 aact:AA03008|5end_GTP-binding_protein_era_homolog_(GTPase)_2100087..2100317_POSITIVE 251 79 35 126 78 aact:AA01763|5end_conserved_hypothetical_protein_(possible_transcriptional_regulatory_protein)_1190643..1190873_POSITIVE 241 48 10 121 68 aact:AA01586|5end_DNA_mismatch_repair_protein_1066966..1067196_POSITIVE 239 68 21 159 105 aact:AA01957|5end_conserved_hypothetical_protein_1323211..1323441_POSITIVE 239 41 11 58 31 aact:AA00449|5end_conserved_hypothetical_protein_312996..313226_POSITIVE 237 57 13 224 185 aact:AA01730|5end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_1168725..1168955_POSITIVE 237 57 13 84 45 aact:AA01729|5end_hypothetical_protein_1168585..1168815_POSITIVE 232 38 7 41 13 aact:AA01158|5end_ribosome_associated_heat_shock_protein_15_homolog_792399..792629_POSITIVE 229 41 2 51 5 aact:AA01367|5end_tRNA_(Guanine-N(1)-)-methyltransferase_(M1G-methyltransferase)_923710..923940_POSITIVE 229 48 1 157 115 aact:AA00796|5end_iron(III)_dicitrate_transport_system_permease_protein_545686..545916_POSITIVE 229 110 64 171 131 aact:AA00170|5end_hypothetical_protein_121238..121468_POSITIVE 228 48 11 192 153 aact:AA02847|5end_conserved_hypothetical_protein_1999276..1999506_POSITIVE 227 80 31 107 51 aact:AA02663|5end_DNA_ligase_1853564..1853794_POSITIVE 225 80 31 228 179 aact:AA03014|5end_conserved_hypothetical_protein_2104815..2105045_POSITIVE 225 48 5 219 157 aact:AA01635|5end_ribonucleoside_diphosphate_reductase,_beta_chain_1104615..1104845_POSITIVE 224 50 4 172 115 aact:AA00738|5end_hypothetical_protein_505087..505317_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 424 59 12 125 78 aact:AA02148|3end_hypothetical_protein_1469642..1469792_POSITIVE 398 110 61 109 61 aact:AA01760|3end_hydrogenase_isoenzymes_formation_protein_1190415..1190565_POSITIVE 317 67 29 121 86 aact:AA01718|3end_DNA_polymerase_III,_chi_subunit_1163316..1163466_POSITIVE 269 48 4 124 82 aact:AA02103|3end_D-alanyl-D-alanine-endopeptidase;_penicillin-binding_protein_7_1431784..1431934_POSITIVE 257 50 8 139 98 aact:AA03006|3end_ribonuclease_III_2100167..2100317_POSITIVE 248 68 21 102 60 aact:AA01705|3end_beta-galactosidase_1157980..1158130_POSITIVE 242 48 3 121 73 aact:AA01690|3end_penicillin-binding_protein_1C_1149142..1149292_POSITIVE 241 48 10 148 95 aact:AA01585|3end_tRNA_delta(2)-isopentenylpyrophosphate_1067073..1067223_POSITIVE 239 41 11 40 13 aact:AA00448|3end_C-terminal_region_of_competence_protein_M_313058..313208_POSITIVE 237 57 13 84 45 aact:AA01728|3end_conserved_hypothetical_protein(related_to_HipA_protein)_1168665..1168815_POSITIVE 230 74 35 150 108 aact:AA01123|3end_possible_hemolysin;_membrane_protein_771657..771807_POSITIVE 228 48 11 133 94 aact:AA02846|3end_crossover_junction_endodeoxyribonuclease_1999297..1999447_POSITIVE 227 80 31 59 3 aact:AA02662|3end_hypothetical_protein_1853596..1853746_POSITIVE 224 50 4 148 91 aact:AA00737|3end_citrate_lyase,_alpha_chain_(citrase)_505143..505293_POSITIVE 223 49 7 38 3 aact:AA02568|3end_sugar_phosphotransferase_component_II_B_1788725..1788875_POSITIVE 221 77 32 146 98 aact:AA01590|3end_hypothetical_protein_1070971..1071121_POSITIVE 221 73 32 138 90 aact:AA01249|3end_conserved_hypothetical_protein_(possible_phosphoesterase)_850423..850573_POSITIVE 219 63 22 66 25 aact:AA00883|3end_hypothetical_protein_614467..614617_POSITIVE 218 49 12 110 70 aact:AA01083|3end_preprotein_translocase_SecA_subunit_750970..751120_POSITIVE 215 39 9 119 94 aact:AA00894|3end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_621350..621500_POSITIVE