Origin IGS: atttttgagttgtcatttttgtatctcctgccgcgcgttaatcacgtatatgccaatcaatagctgtttttttagtttccatttatgtcctcattagataacttcattcgttaatcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagattaacgaatgaagttatctaatgaggacataaatggaaactaaaaaaacagctattgattggcatatacgtgattaacgcgcggcaggagatacaaaaatgacaactcaaaaat Mask Tandem Repeat Region ================================================ atttttgagttgtcatttttgtatctcctgccgcgcgttaatcacgtatatgccaatcaatagctgtttttttagtttccatttatgtcctcattagataacttcattcgttaatcta Find is-nt database================================================ Query_seq: AA01318:AA01319|AA01318:AA01319:hypothetical protein:transcriptional regulatory protein:->->:892474..892591 118 atttttgagttgtcatttttgtatctcctgccgcgcgttaatcacgtatatgccaatcaatagctgtttttttagtttccatttatgtcctcattagataacttcattcgttaatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01318:AA01319|AA01318:AA01319:hypothetical protein:transcriptional regulatory protein:->->:892474..892591 118 atttttgagttgtcatttttgtatctcctgccgcgcgttaatcacgtatatgccaatcaatagctgtttttttagtttccatttatgtcctcattagataacttcattcgttaatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01318:AA01319|AA01318:AA01319:hypothetical protein:transcriptional regulatory protein:->->:892474..892591 118 atttttgagttgtcatttttgtatctcctgccgcgcgttaatcacgtatatgccaatcaatagctgtttttttagtttccatttatgtcctcattagataacttcattcgttaatcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atttttgagttgtcatttttgtatctcctgccgcgcgttaatcacgtatatgccaatcaatagctgtttttttagtttccatttatgtcctcattagataacttcattcgttaatcta Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 5 End: 94 .... M S C H F C I S C R A L I T Y M P I N S C F F S F H L C P H ........................ atttttgagttgtcatttttgtatctcctgccgcgcgttaatcacgtatatgccaatcaatagctgtttttttagtttccatttatgtcctcattagataacttcattcgttaatcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atttttgagttgtcatttttgtatctcctgccgcgcgttaatcacgtatatgccaatcaatagctgtttttttagtttccatttatgtcctcattagataacttcattcgttaatcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01318:AA01319|AA01318:AA01319:hypothetical protein:transcriptional regulatory protein:->->:892474..892591 118 atttttgagttgtcatttttgtatctcctgccgcgcgttaatcacgtatatgccaatcaatagctgtttttttagtttccatttatgtcctcattagataacttcattcgttaatcta Intra-Species Hit: Count: 1 Min: 1 Max: 118 Len: 118 Subject: aact_AA01318_AA01319|hypothetical protein:transcriptional regulatory protein|POSITIVE:POSITIVE|[892474,892591]|118 HSP 1 e-value: 2.0E-48 bit: 192.0 Len: 118 Query Start:1 Query End:118 Subject Strand: POSITIVE Subject Start: 1 Subject End: 118 atttttgagttgtcatttttgtatctcctgccgcgcgttaatcacgtatatgccaatcaatagctgnnnnnnnagtttccatttatgtcctcattagataacttcattcgttaatcta atttttgagttgtcatttttgtatctcctgccgcgcgttaatcacgtatatgccaatcaatagctgtttttttagtttccatttatgtcctcattagataacttcattcgttaatcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUUUGAGUUGUCAUUUUUGUAUCUCCUGCCGCGCGUUAAUCACGUAUAUGCCAAUCAAUAGCUGUUUUUUUAGUUUCCAUUUAUGUCCUCAUUAGAUAACUUCAUUCGUUAAUCUA ......((((((((...............((...((((.....))))....))........(((((......)))))...................)))))))).............. (-12.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAUUAACGAAUGAAGUUAUCUAAUGAGGACAUAAAUGGAAACUAAAAAAACAGCUAUUGAUUGGCAUAUACGUGAUUAACGCGCGGCAGGAGAUACAAAAAUGACAACUCAAAAAU (((((.(((.......)))))))).((((..(((....(....)..........((((.....)))).....((((.......))))...............)))....))))..... (-16.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 355 70 31 56 8 aact:AA01141|5end_conserved_hypothetical_protein_(possible_component_of_anaerobic_dehydrogenase/oxidoreductase)_782497..782727_POSITIVE 316 63 23 158 121 aact:AA01303|5end_competence_protein_A_881996..882226_POSITIVE 313 54 11 180 135 aact:AA00805|5end_fumarate_reductase_iron-sulfur_protein_551583..551813_POSITIVE 308 55 27 179 148 aact:AA00887|5end_hypothetical_protein_616739..616969_POSITIVE 306 69 27 198 153 aact:AA01337|5end_cell_division_protein_N_904235..904465_POSITIVE 302 50 5 122 62 aact:AA01628|5end_thiamin-monophosphate_kinase_1100888..1101118_POSITIVE 300 70 21 181 121 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 294 70 21 198 138 aact:AA01748|5end_thiol:disulfide_interchange_protein_1183062..1183292_POSITIVE 294 59 16 192 146 aact:AA01338|5end_hypothetical_protein_904967..905197_POSITIVE 291 39 18 229 211 aact:AA01767|5end_conserved_hypothetical_protein_1192528..1192758_POSITIVE 291 44 2 200 158 aact:AA01557|5end_recombinase_A_1044911..1045141_POSITIVE 289 55 11 58 4 aact:AA01429|5end_conserved_hypothetical_protein_959581..959811_POSITIVE 289 66 28 184 149 aact:AA00796|5end_iron(III)_dicitrate_transport_system_permease_protein_545686..545916_POSITIVE 283 66 24 215 177 aact:AA01615|5end_lipoprotein_releasing_system_transmembrane_1091186..1091416_POSITIVE 282 65 27 221 185 aact:AA01565|5end_conserved_hypothetical_protein_1050711..1050941_POSITIVE 281 55 15 85 34 aact:AA01567|5end_conserved_hypothetical_protein_(possible_cell-wall-associated_hydrolase)_1054944..1055174_POSITIVE 281 55 15 85 34 aact:AA01262|5end_hydrolase_859576..859806_POSITIVE 280 59 27 75 43 aact:AA00259|5end_conserved_hypothetical_protein_182788..183018_POSITIVE 279 64 29 217 179 aact:AA02947|5end_riboflavin_biosynthesis_protein_2060871..2061101_POSITIVE 273 53 16 205 167 aact:AA02693|5end_hydrogenase-4_component_H_1877921..1878151_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 302 50 5 100 40 aact:AA01626|3end_N_utilization_substance_protein_B_1100946..1101096_POSITIVE 285 55 12 151 98 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 280 59 27 59 27 aact:AA00258|3end_penicillin_binding_protein_2_182852..183002_POSITIVE 279 64 29 136 98 aact:AA02946|3end_virulence_factor_protein_2060870..2061020_POSITIVE 270 110 65 72 30 aact:AA00427|3end_hypothetical_protein_289956..290106_POSITIVE 267 66 29 56 23 aact:AA01747|3end_single-stranded-DNA-specific_exonuclease_1183130..1183280_POSITIVE 267 50 1 96 35 aact:AA00544|3end_glutathionylspermidine_synthetase;amidase_373733..373883_POSITIVE 266 56 11 145 98 aact:AA02950|3end_isoleucyl-tRNA_synthetase_2065113..2065263_POSITIVE 266 64 24 72 28 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE 264 80 31 77 20 aact:AA02046|3end_PTS_system_galactitol-specific_enzyme_IIC_1393687..1393837_POSITIVE 263 39 5 60 15 aact:AA02152|3end_ferric_enterobactin_transport;_ABC_transporter_ATP-binding_protein_1472610..1472760_POSITIVE 259 66 28 110 65 aact:AA02462|3end_thiol_peroxidase_(scavengase)_1702673..1702823_POSITIVE 259 54 13 144 93 aact:AA01796|3end_branched-chain_amino_acid_aminotransferase_1212168..1212318_POSITIVE 257 69 24 63 13 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 254 46 7 54 13 aact:AA01746|3end_thiol:disulfide_interchange_protein_1181396..1181546_POSITIVE 254 54 21 58 18 aact:AA01260|3end_hypothetical_protein_855758..855908_POSITIVE 253 79 31 147 99 aact:AA01439|3end_probable_ferredoxin_(fdx-2)_966498..966648_POSITIVE 252 54 15 61 34 aact:AA01759|3end_hydrogenase_isoenzymes_formation_protein_1189864..1190014_POSITIVE 252 68 31 44 7 aact:AA00694|3end_dCTP_deaminase_(deoxycytidine_triphosphate_deaminas)_475100..475250_POSITIVE 250 66 29 129 92 aact:AA01711|3end_conserved_hypothetical_protein_1160502..1160652_POSITIVE