Origin IGS: taaacgatggcttgattaaacaaaaggtctgaaagtgttcagaccttttgtttagccattaggcagaaaaacgcctaagaaaaaaaccgcacttgagtcgatcaacataaagtgtggtttcttttaaagatgttttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaaacatctttaaaagaaaccacactttatgttgatcgactcaagtgcggtttttttcttaggcgtttttctgcctaatggctaaacaaaaggtctgaacactttcagaccttttgtttaatcaagccatcgttta Mask Tandem Repeat Region ================================================ taaacgatggcttgattaaacaaaaggtctgaaagtgttcagaccttttgtttagccattaggcagaaaaacgcctaagaaaaaaaccgcacttgagtcgatcaacataaagtgtggtttcttttaaagatgttttta Find is-nt database================================================ Query_seq: AA01306:AA01308|AA01306:AA01308:glutathione reductase:cyclic AMP receptor protein:->->:888046..888183 138 taaacgatggcttgattaaacaaaaggtctgaaagtgttcagaccttttgtttagccattaggcagaaaaacgcctaagaaaaaaaccgcacttgagtcgatcaacataaagtgtggtttcttttaaagatgttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01306:AA01308|AA01306:AA01308:glutathione reductase:cyclic AMP receptor protein:->->:888046..888183 138 taaacgatggcttgattaaacaaaaggtctgaaagtgttcagaccttttgtttagccattaggcagaaaaacgcctaagaaaaaaaccgcacttgagtcgatcaacataaagtgtggtttcttttaaagatgttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01306:AA01308|AA01306:AA01308:glutathione reductase:cyclic AMP receptor protein:->->:888046..888183 138 taaacgatggcttgattaaacaaaaggtctgaaagtgttcagaccttttgtttagccattaggcagaaaaacgcctaagaaaaaaaccgcacttgagtcgatcaacataaagtgtggtttcttttaaagatgttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaacgatggcttgattaaacaaaaggtctgaaagtgttcagaccttttgtttagccattaggcagaaaaacgcctaagaaaaaaaccgcacttgagtcgatcaacataaagtgtggtttcttttaaagatgttttta Predict ORF larger than 30AA ================================================ taaacgatggcttgattaaacaaaaggtctgaaagtgttcagaccttttgtttagccattaggcagaaaaacgcctaagaaaaaaaccgcacttgagtcgatcaacataaagtgtggtttcttttaaagatgttttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaacgatggcttgattaaacaaaaggtctgaaagtgttcagaccttttgtttagccattaggcagaaaaacgcctaagaaaaaaaccgcacttgagtcgatcaacataaagtgtggtttcttttaaagatgttttta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 100 HP_score: -16.7 Tail_Score: -3.72893 Start: 21 End: 50 Full_Region: tgcctaatggctaaa caaaaggtctgaa cact ttcagaccttttg tttaatcaagccatc ....................caaaaggtctgaacactttcagaccttttg........................................................................................ TransTerm Strand: - Conf: 93 HP_score: -10.8 Tail_Score: -4.93877 Start: 26 End: 45 Full_Region: aatggctaaacaaaa ggtctgaa cact ttcagacc ttttgtttaatcaag .........................ggtctgaacactttcagacc............................................................................................. Find igs database================================================ Query_seq: AA01306:AA01308|AA01306:AA01308:glutathione reductase:cyclic AMP receptor protein:->->:888046..888183 138 taaacgatggcttgattaaacaaaaggtctgaaagtgttcagaccttttgtttagccattaggcagaaaaacgcctaagaaaaaaaccgcacttgagtcgatcaacataaagtgtggtttcttttaaagatgttttta Intra-Species Hit: Count: 1 Min: 1 Max: 138 Len: 138 Subject: aact_AA01306_AA01308|glutathione reductase:cyclic AMP receptor protein|POSITIVE:POSITIVE|[888046,888183]|138 HSP 1 e-value: 2.0E-60 bit: 232.0 Len: 138 Query Start:1 Query End:138 Subject Strand: POSITIVE Subject Start: 1 Subject End: 138 taaacgatggcttgattaaacaaaaggtctgaaagtgttcagaccttttgtttagccattaggcagaaaaacgcctaagnnnnnnnccgcacttgagtcgatcaacataaagtgtggtttcttttaaagatgttttta taaacgatggcttgattaaacaaaaggtctgaaagtgttcagaccttttgtttagccattaggcagaaaaacgcctaagaaaaaaaccgcacttgagtcgatcaacataaagtgtggtttcttttaaagatgttttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAACGAUGGCUUGAUUAAACAAAAGGUCUGAAAGUGUUCAGACCUUUUGUUUAGCCAUUAGGCAGAAAAACGCCUAAGAAAAAAACCGCACUUGAGUCGAUCAACAUAAAGUGUGGUUUCUUUUAAAGAUGUUUUUA .....(((((((.....((((((((((((((((....)))))))))))))))))))))))......(((((((.((..((((.(((((((((((..((......))...))))))))))).))))..)).))))))). (-50.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAACAUCUUUAAAAGAAACCACACUUUAUGUUGAUCGACUCAAGUGCGGUUUUUUUCUUAGGCGUUUUUCUGCCUAAUGGCUAAACAAAAGGUCUGAACACUUUCAGACCUUUUGUUUAAUCAAGCCAUCGUUUA ...((((.......(((((((((.(((((...(((....)))..))))).))))))))).(((((((......))))))).((((((((((((((((((((....)))))))))))))))).....))))...)))). (-50.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 443 120 71 150 101 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 411 120 72 148 99 aact:AA02762|5end_ribonuclease_H_1933164..1933394_POSITIVE 404 108 62 90 36 aact:AA01303|5end_competence_protein_A_881996..882226_POSITIVE 386 119 71 152 109 aact:AA00454|5end_conserved_hypothetical_protein_318020..318250_POSITIVE 371 118 71 227 168 aact:AA02367|5end_conserved_hypothetical_protein_1623174..1623404_POSITIVE 371 118 71 73 14 aact:AA02366|5end_hypothetical_protein_1623020..1623250_POSITIVE 361 118 71 148 103 aact:AA02980|5end_conserved_hypothetical_protein_2083305..2083535_POSITIVE 358 118 71 145 98 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 354 108 62 185 132 aact:AA01458|5end_aminotransferase_978820..979050_POSITIVE 353 99 54 82 33 aact:AA00952|5end_hemY_protein_homolog_657541..657771_POSITIVE 349 118 71 151 102 aact:AA01436|5end_fructose-bisphosphate_aldolase_class_II_962981..963211_POSITIVE 349 120 71 149 98 aact:AA00792|5end_conserved_hypothetical_protein_543142..543372_POSITIVE 345 118 71 154 108 aact:AA00075|5end_conserved_hypothetical_protein_54963..55193_POSITIVE 344 119 71 147 99 aact:AA02252|5end_conserved_hypothetical_protein_1533840..1534070_POSITIVE 344 118 71 226 188 aact:AA00347|5end_50S_ribosomal_protein_L11_232137..232367_POSITIVE 341 120 71 147 100 aact:AA00987|5end_tetracenomycin_polyketide_synthesis_O-methyltransferase_689745..689975_POSITIVE 334 97 52 82 37 aact:AA02123|5end_hypothetical_protein_1448244..1448474_POSITIVE 334 118 71 145 98 aact:AA01704|5end_tyrosine-specific_transport_protein_1156170..1156400_POSITIVE 333 120 71 148 98 aact:AA02937|5end_conserved_hypothetical_protein_2056379..2056609_POSITIVE 333 119 72 144 101 aact:AA01936|5end_tyrosyl-tRNA_synthetase_1307043..1307273_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 505 59 11 84 28 aact:AA01306|3end_glutathione_reductase_887985..888135_POSITIVE 411 120 72 54 5 aact:AA02758|3end_3-phosphoshikimate_1-carboxyvinyltransferase_1933150..1933300_POSITIVE 404 108 62 109 55 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 386 119 71 61 18 aact:AA00453|3end_phosphoglyceromutase_318009..318159_POSITIVE 373 118 71 99 54 aact:AA02135|3end_hypothetical_protein_1459067..1459217_POSITIVE 373 118 71 115 70 aact:AA02135.1|3end_hypothetical_protein_1459083..1459233_POSITIVE 361 118 71 71 26 aact:AA02978|3end_sufI_protein_precursor_homolog_2083308..2083458_POSITIVE 354 108 62 93 40 aact:AA01457|3end_D-xylose_ABC_transporter,_membrane_spanning_protein_978808..978958_POSITIVE 349 118 71 111 62 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE 349 120 71 61 10 aact:AA00790|3end_recombination_DNA_repair_protein_543134..543284_POSITIVE 345 118 71 58 12 aact:AA00072|3end_conserved_hypothetical_protein_54947..55097_POSITIVE 344 119 71 70 22 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE 344 118 71 67 29 aact:AA00345|3end_transcription_antitermination_protein_232058..232208_POSITIVE 341 120 71 63 16 aact:AA00985|3end_transcriptional_regulator_(LysR_family)_689741..689891_POSITIVE 338 101 66 69 32 aact:AA01945|3end_selenophosphate_synthase_1316022..1316172_POSITIVE 334 118 71 56 9 aact:AA01703|3end_L-ribulose-phosphate_4-epimerase_1156161..1156311_POSITIVE 333 120 71 62 12 aact:AA02936|3end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2056373..2056523_POSITIVE 333 118 74 62 18 aact:AA00974|3end_glucosamine-fructose-6-phosphate_681127..681277_POSITIVE 330 120 87 34 1 aact:AA01690|3end_penicillin-binding_protein_1C_1149142..1149292_POSITIVE 326 94 53 146 100 aact:AA01314|3end_hypothetical_protein_890196..890346_POSITIVE