Origin IGS: taagtaaaacgctcgaaaaaacgaccgcacttttcgcaatcgcaaggtggggctcaacccactgaaacagtgttgaatgaaatggtaggctaaaacccaccataaaataaaaggaaattaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttaatttccttttattttatggtgggttttagcctaccatttcattcaacactgtttcagtgggttgagccccaccttgcgattgcgaaaagtgcggtcgttttttcgagcgttttactta Mask Tandem Repeat Region ================================================ taagtaaaacgctcgaaaaaacgaccgcacttttcgcaatcgcaaggtggggctcaacccactgaaacagtgttgaatgaaatggtaggctaaaacccaccataaaataaaaggaaattaaaa Find is-nt database================================================ Query_seq: AA01280:AA01284|AA01280:AA01284:groES chaperonin:heat shock protein (hsp60):->->:868591..868713 123 taagtaaaacgctcgaaaaaacgaccgcacttttcgcaatcgcaaggtggggctcaacccactgaaacagtgttgaatgaaatggtaggctaaaacccaccataaaataaaaggaaattaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01280:AA01284|AA01280:AA01284:groES chaperonin:heat shock protein (hsp60):->->:868591..868713 123 taagtaaaacgctcgaaaaaacgaccgcacttttcgcaatcgcaaggtggggctcaacccactgaaacagtgttgaatgaaatggtaggctaaaacccaccataaaataaaaggaaattaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01280:AA01284|AA01280:AA01284:groES chaperonin:heat shock protein (hsp60):->->:868591..868713 123 taagtaaaacgctcgaaaaaacgaccgcacttttcgcaatcgcaaggtggggctcaacccactgaaacagtgttgaatgaaatggtaggctaaaacccaccataaaataaaaggaaattaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagtaaaacgctcgaaaaaacgaccgcacttttcgcaatcgcaaggtggggctcaacccactgaaacagtgttgaatgaaatggtaggctaaaacccaccataaaataaaaggaaattaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 39 Strand: - Start: 2 End: 118 . L L V S S F V V A S K A I A L H P E V W Q F L T S H F P L S F G V M F Y F S M ..... Protein_Len: 36 Strand: - Start: 1 End: 108 L Y F A R F F S R V K R L R L T P S L G S F C H Q I F H Y A L V W W L M ............... taagtaaaacgctcgaaaaaacgaccgcacttttcgcaatcgcaaggtggggctcaacccactgaaacagtgttgaatgaaatggtaggctaaaacccaccataaaataaaaggaaattaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagtaaaacgctcgaaaaaacgaccgcacttttcgcaatcgcaaggtggggctcaacccactgaaacagtgttgaatgaaatggtaggctaaaacccaccataaaataaaaggaaattaaaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 72 HP_score: -3.2 Tail_Score: -5.03747 Start: 22 End: 42 Full_Region: ttgagccccaccttg cgattgcg aaaag tgcggtcg ttttttcgagcgttt .....................cgattgcgaaaagtgcggtcg................................................................................. Find igs database================================================ Query_seq: AA01280:AA01284|AA01280:AA01284:groES chaperonin:heat shock protein (hsp60):->->:868591..868713 123 taagtaaaacgctcgaaaaaacgaccgcacttttcgcaatcgcaaggtggggctcaacccactgaaacagtgttgaatgaaatggtaggctaaaacccaccataaaataaaaggaaattaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 123 Len: 123 Subject: aact_AA01280_AA01284|groES chaperonin:heat shock protein (hsp60)|POSITIVE:POSITIVE|[868591,868713]|123 HSP 1 e-value: 6.0E-64 bit: 244.0 Len: 123 Query Start:1 Query End:123 Subject Strand: POSITIVE Subject Start: 1 Subject End: 123 taagtaaaacgctcgaaaaaacgaccgcacttttcgcaatcgcaaggtggggctcaacccactgaaacagtgttgaatgaaatggtaggctaaaacccaccataaaataaaaggaaattaaaa taagtaaaacgctcgaaaaaacgaccgcacttttcgcaatcgcaaggtggggctcaacccactgaaacagtgttgaatgaaatggtaggctaaaacccaccataaaataaaaggaaattaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGUAAAACGCUCGAAAAAACGACCGCACUUUUCGCAAUCGCAAGGUGGGGCUCAACCCACUGAAACAGUGUUGAAUGAAAUGGUAGGCUAAAACCCACCAUAAAAUAAAAGGAAAUUAAAA .......((((((.(......(((..((.......))..)))...((((((......))))))....))))))).......(((((.((......)).))))).................... (-25.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAAUUUCCUUUUAUUUUAUGGUGGGUUUUAGCCUACCAUUUCAUUCAACACUGUUUCAGUGGGUUGAGCCCCACCUUGCGAUUGCGAAAAGUGCGGUCGUUUUUUCGAGCGUUUUACUUA ......................((((((.((((((((((.....((........)).....)))))))))).))))))..(((((((((.....))))))))).................... (-33.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 491 50 1 228 179 aact:AA00347|5end_50S_ribosomal_protein_L11_232137..232367_POSITIVE 449 50 5 164 109 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 436 50 6 147 98 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 425 50 6 145 95 aact:AA02937|5end_conserved_hypothetical_protein_2056379..2056609_POSITIVE 416 50 2 154 107 aact:AA00409|5end_methionyl-tRNA_formyltransferase_273611..273841_POSITIVE 408 37 2 126 89 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 406 50 6 144 95 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 404 50 6 175 124 aact:AA00386|5end_aspartate--ammonia_ligase_(asparagine_synthetase_A)_261150..261380_POSITIVE 400 44 6 90 48 aact:AA02965|5end_hypothetical_protein_2074069..2074299_POSITIVE 384 43 6 162 127 aact:AA02409|5end_DNA_ligase_1653315..1653545_POSITIVE 380 54 13 158 111 aact:AA00464|5end_conserved_hypothetical_protein_323912..324142_POSITIVE 376 50 7 72 24 aact:AA02846|5end_crossover_junction_endodeoxyribonuclease_1998648..1998878_POSITIVE 376 43 10 97 58 aact:AA00908|5end_probable_sigma-E_factor_regulatory_protein_626076..626306_POSITIVE 375 43 7 50 18 aact:AA01615|5end_lipoprotein_releasing_system_transmembrane_1091186..1091416_POSITIVE 371 70 22 193 142 aact:AA00596|5end_uridylate_kinase_407496..407726_POSITIVE 370 50 6 187 135 aact:AA00239|5end_hypothetical_protein_171213..171443_POSITIVE 363 49 6 143 95 aact:AA00792|5end_conserved_hypothetical_protein_543142..543372_POSITIVE 362 69 22 203 144 aact:AA02691|5end_hypothetical_protein_1876137..1876367_POSITIVE 361 47 4 136 91 aact:AA01181|5end_conserved_hypothetical_protein_808774..809004_POSITIVE 360 47 6 142 95 aact:AA01537|5end_possible_glucokinase_regulatory_protein_1030616..1030846_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 491 50 1 69 20 aact:AA00345|3end_transcription_antitermination_protein_232058..232208_POSITIVE 449 50 5 59 4 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 425 50 6 59 9 aact:AA02936|3end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2056373..2056523_POSITIVE 416 50 2 151 104 aact:AA00408|3end_SUN_protein_(FMU_protein)_273688..273838_POSITIVE 404 50 6 113 62 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 400 44 6 69 27 aact:AA02964|3end_opacity_associated_protein_A_2074128..2074278_POSITIVE 380 54 13 64 17 aact:AA00463|3end_class_B_acid_phosphatase_323898..324048_POSITIVE 376 43 10 84 45 aact:AA00906|3end_sigma-E_factor_regulatory_protein_626143..626293_POSITIVE 375 47 6 84 48 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE 370 50 6 63 11 aact:AA00237|3end_hypothetical_protein_171169..171319_POSITIVE 363 41 6 138 102 aact:AA01259|3end_protoporphyrinogen_oxidase_854943..855093_POSITIVE 363 49 6 55 7 aact:AA00790|3end_recombination_DNA_repair_protein_543134..543284_POSITIVE 361 47 4 83 38 aact:AA01179|3end_beta-ketoacyl-ACP_reductase;_short-chain_alcohol_dehydrogenase_808801..808951_POSITIVE 359 48 6 50 13 aact:AA02704|3end_dihydrolipoamide_succinyltransferase_1886795..1886945_POSITIVE 358 32 2 59 27 aact:AA00112|3end_uracil_permease_81239..81389_POSITIVE 355 53 13 144 99 aact:AA02184|3end_anthranilate_isomerase;_indole-3-glycerol_phosphate_synthase;_phosphoribosylanthranilate_isomerase_1492541..1492691_POSITIVE 352 48 6 108 61 aact:AA01992|3end_protease_IV;_signal_peptide_peptidase;_Endopeptidase_IV_1355004..1355154_POSITIVE 347 55 13 102 68 aact:AA02090|3end_glucose-1-phosphate_adenylyltransferase_1424549..1424699_POSITIVE 344 50 6 80 37 aact:AA02136|3end_aspartate_aminotransferase_1460476..1460626_POSITIVE 344 50 3 56 8 aact:AA01023|3end_conserved_hypothetical_protein_716435..716585_POSITIVE