Origin IGS: ttacgtcgattttttcatgatggacaacaggttaccttatgaagtggttgatgtgttgaattagctccttagcggagatttgtttaggcagaaccggtaaagattgggatagtgcgcccatcttacttaacaaatcacggattatgtattgatgaaaaagctacaggttctgcctcatacgaattagtctgccaaatctctttgggtcgtttgaacccgtttcgttcggc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gccgaacgaaacgggttcaaacgacccaaagagatttggcagactaattcgtatgaggcagaacctgtagctttttcatcaatacataatccgtgatttgttaagtaagatgggcgcactatcccaatctttaccggttctgcctaaacaaatctccgctaaggagctaattcaacacatcaaccacttcataaggtaacctgttgtccatcatgaaaaaatcgacgtaa Mask Tandem Repeat Region ================================================ ttacgtcgattttttcatgatggacaacaggttaccttatgaagtggttgatgtgttgaattagctccttagcggagatttgtttaggcagaaccggtaaagattgggatagtgcgcccatcttacttaacaaatcacggattatgtattgatgaaaaagctacaggttctgcctcatacgaattagtctgccaaatctctttgggtcgtttgaacccgtttcgttcggc Find is-nt database================================================ Query_seq: AA01272:AA01273|AA01272:AA01273:hypothetical protein:hypothetical protein:->->:864272..864501 230 ttacgtcgattttttcatgatggacaacaggttaccttatgaagtggttgatgtgttgaattagctccttagcggagatttgtttaggcagaaccggtaaagattgggatagtgcgcccatcttacttaacaaatcacggattatgtattgatgaaaaagctacaggttctgcctcatacgaattagtctgccaaatctctttgggtcgtttgaacccgtttcgttcggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01272:AA01273|AA01272:AA01273:hypothetical protein:hypothetical protein:->->:864272..864501 230 ttacgtcgattttttcatgatggacaacaggttaccttatgaagtggttgatgtgttgaattagctccttagcggagatttgtttaggcagaaccggtaaagattgggatagtgcgcccatcttacttaacaaatcacggattatgtattgatgaaaaagctacaggttctgcctcatacgaattagtctgccaaatctctttgggtcgtttgaacccgtttcgttcggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01272:AA01273|AA01272:AA01273:hypothetical protein:hypothetical protein:->->:864272..864501 230 ttacgtcgattttttcatgatggacaacaggttaccttatgaagtggttgatgtgttgaattagctccttagcggagatttgtttaggcagaaccggtaaagattgggatagtgcgcccatcttacttaacaaatcacggattatgtattgatgaaaaagctacaggttctgcctcatacgaattagtctgccaaatctctttgggtcgtttgaacccgtttcgttcggc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttacgtcgattttttcatgatggacaacaggttaccttatgaagtggttgatgtgttgaattagctccttagcggagatttgtttaggcagaaccggtaaagattgggatagtgcgcccatcttacttaacaaatcacggattatgtattgatgaaaaagctacaggttctgcctcatacgaattagtctgccaaatctctttgggtcgtttgaacccgtttcgttcggc Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 60 End: 149 ........................................................... M S S L A E I C L G R T G K D W D S A P I L L N K S R I M Y ................................................................................. Protein_Len: 42 Strand: + Start: 103 End: 228 ...................................................................................................... M G I V R P S Y L T N H G L C I D E K A T G S A S Y E L V C Q I S L G R L N P F R S .. Protein_Len: 37 Strand: - Start: 35 End: 145 .................................. R I F H N I H Q I L E K A S I Q K P L V P L S Q S L A G M K S L L D R I M ..................................................................................... ttacgtcgattttttcatgatggacaacaggttaccttatgaagtggttgatgtgttgaattagctccttagcggagatttgtttaggcagaaccggtaaagattgggatagtgcgcccatcttacttaacaaatcacggattatgtattgatgaaaaagctacaggttctgcctcatacgaattagtctgccaaatctctttgggtcgtttgaacccgtttcgttcggc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttacgtcgattttttcatgatggacaacaggttaccttatgaagtggttgatgtgttgaattagctccttagcggagatttgtttaggcagaaccggtaaagattgggatagtgcgcccatcttacttaacaaatcacggattatgtattgatgaaaaagctacaggttctgcctcatacgaattagtctgccaaatctctttgggtcgtttgaacccgtttcgttcggc Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01272:AA01273|AA01272:AA01273:hypothetical protein:hypothetical protein:->->:864272..864501 230 ttacgtcgattttttcatgatggacaacaggttaccttatgaagtggttgatgtgttgaattagctccttagcggagatttgtttaggcagaaccggtaaagattgggatagtgcgcccatcttacttaacaaatcacggattatgtattgatgaaaaagctacaggttctgcctcatacgaattagtctgccaaatctctttgggtcgtttgaacccgtttcgttcggc Intra-Species Hit: Count: 1 Min: 1 Max: 230 Len: 230 Subject: aact_AA01272_AA01273|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[864272,864501]|230 HSP 1 e-value: 1.0E-127 bit: 456.0 Len: 230 Query Start:1 Query End:230 Subject Strand: POSITIVE Subject Start: 1 Subject End: 230 ttacgtcgattttttcatgatggacaacaggttaccttatgaagtggttgatgtgttgaattagctccttagcggagatttgtttaggcagaaccggtaaagattgggatagtgcgcccatcttacttaacaaatcacggattatgtattgatgaaaaagctacaggttctgcctcatacgaattagtctgccaaatctctttgggtcgtttgaacccgtttcgttcggc ttacgtcgattttttcatgatggacaacaggttaccttatgaagtggttgatgtgttgaattagctccttagcggagatttgtttaggcagaaccggtaaagattgggatagtgcgcccatcttacttaacaaatcacggattatgtattgatgaaaaagctacaggttctgcctcatacgaattagtctgccaaatctctttgggtcgtttgaacccgtttcgttcggc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUACGUCGAUUUUUUCAUGAUGGACAACAGGUUACCUUAUGAAGUGGUUGAUGUGUUGAAUUAGCUCCUUAGCGGAGAUUUGUUUAGGCAGAACCGGUAAAGAUUGGGAUAGUGCGCCCAUCUUACUUAACAAAUCACGGAUUAUGUAUUGAUGAAAAAGCUACAGGUUCUGCCUCAUACGAAUUAGUCUGCCAAAUCUCUUUGGGUCGUUUGAACCCGUUUCGUUCGGC ..((((((((..((((((((..(((.....)))...))))))))..))))))))(((((((..........((((((((((((..((((((((((.((((((((.(((........))))))))..........(((..(((.....)))..)))......))).))))))))))...)))))))..)))))...........((((.......)))).....))))))) (-65.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GCCGAACGAAACGGGUUCAAACGACCCAAAGAGAUUUGGCAGACUAAUUCGUAUGAGGCAGAACCUGUAGCUUUUUCAUCAAUACAUAAUCCGUGAUUUGUUAAGUAAGAUGGGCGCACUAUCCCAAUCUUUACCGGUUCUGCCUAAACAAAUCUCCGCUAAGGAGCUAAUUCAACACAUCAACCACUUCAUAAGGUAACCUGUUGUCCAUCAUGAAAAAAUCGACGUAA (((((((.....(((((.....))))).....).)))))).......((((((((.(((((((((..((((....((((..((.....))..))))...)))).((((((((((........))).))).)))).))))))))).........((((.....))))......(((((.....(((.........)))....)))))..))).)))))............. (-53.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 354 230 186 107 64 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 351 116 73 80 36 aact:AA01780|5end_ABC-related_molybdenum_transport_system_permease_protein_1201084..1201314_POSITIVE 350 98 53 211 170 aact:AA00649|5end_peptide_chain_release_factor_1_(RF-1)_443277..443507_POSITIVE 348 120 72 182 130 aact:AA00290|5end_conserved_hypothetical_protein_201272..201502_POSITIVE 336 119 72 228 179 aact:AA00675|5end_glutaredoxin_2_462524..462754_POSITIVE 326 119 71 204 159 aact:AA02910|5end_ribonuclease_E_2036137..2036367_POSITIVE 319 116 71 171 124 aact:AA01794|5end_glycine_cleavage_system_transcription_activator_1209708..1209938_POSITIVE 315 119 72 181 128 aact:AA02667|5end_hypothetical_protein_1856609..1856839_POSITIVE 313 230 189 192 138 aact:AA02487|5end_hypothetical_protein_1726277..1726507_POSITIVE 309 97 52 145 97 aact:AA02002|5end_hypothetical_protein_1360196..1360426_POSITIVE 308 90 42 125 72 aact:AA02898|5end_oligopeptide_transport_ATP-binding_protein_2029083..2029313_POSITIVE 308 116 79 64 32 aact:AA01155|5end_hypothetical_protein_791227..791457_POSITIVE 307 118 72 109 51 aact:AA00424|5end_hypothetical_protein_287123..287353_POSITIVE 303 124 82 119 68 aact:AA02776|5end_conserved_hypothetical_protein_1939693..1939923_POSITIVE 303 139 91 95 54 aact:AA01861|5end_phosphomethylpyrimidine_kinase_1251806..1252036_POSITIVE 302 228 188 147 95 aact:AA02693|5end_hydrogenase-4_component_H_1877921..1878151_POSITIVE 301 120 71 228 172 aact:AA00507|5end_hypothetical_protein_352996..353226_POSITIVE 300 125 81 50 1 aact:AA01841|5end_conserved_hypothetical_protein_1237514..1237744_POSITIVE 300 89 41 71 29 aact:AA00414|5end_possible_histidinol-phosphatase_280899..281129_POSITIVE 299 230 187 204 160 aact:AA01417|5end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_953256..953486_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 373 122 81 44 9 aact:AA02954|3end_penicillin_tolerance_protein;_hydroxymethylbutenyl_pyrophosphate_reductase_2066631..2066781_POSITIVE 351 116 73 90 46 aact:AA01779|3end_molybdenum_ABC_transporter,_ATP-binding_protein_1201174..1201324_POSITIVE 328 119 71 62 19 aact:AA01033|3end_Na(+)-translocating_NADH-quinone_reductase_subunit_A_723435..723585_POSITIVE 315 119 72 75 22 aact:AA02666|3end_DNA_helicase_II_1856583..1856733_POSITIVE 313 230 189 147 93 aact:AA02486|3end_topoisomerase_IV,_subunit_B_1726312..1726462_POSITIVE 309 97 52 108 60 aact:AA02001|3end_conserved_hypothetical_protein_1360239..1360389_POSITIVE 308 90 42 112 59 aact:AA02897|3end_oligopeptide_ABC_transporter;_permease_protein_2029150..2029300_POSITIVE 307 118 72 130 72 aact:AA00423|3end_GTP-binding_protein_287224..287374_POSITIVE 303 124 82 69 18 aact:AA02775|3end_S-adenosylmethionine:tRNA_ribosyltransferase-isomerase_(Queuosine_biosynthesis_protein_queA)_1939723..1939873_POSITIVE 303 139 91 99 58 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 302 228 188 130 78 aact:AA02692|3end_hydrogenase_4_subunit;_hydrogenase_3_subunit_1877984..1878134_POSITIVE 298 117 72 101 40 aact:AA02654|3end_probable_lipopolysaccharide_biosynthesis_protein_1849247..1849397_POSITIVE 298 68 26 57 20 aact:AA01654|3end_periplasmic_maltose-binding_protein_precursor_1121163..1121313_POSITIVE 295 230 188 55 18 aact:AA03008|3end_GTP-binding_protein_era_homolog_(GTPase)_2101072..2101222_POSITIVE 295 108 67 148 111 aact:AA01222|3end_hypothetical_protein_834386..834536_POSITIVE 294 118 72 77 33 aact:AA02101|3end_penicillin-binding_protein_7_1431188..1431338_POSITIVE 294 110 61 56 1 aact:AA00876|3end_tight_adherence_protein_C_608704..608854_POSITIVE 291 230 190 126 92 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 289 230 186 150 108 aact:AA01396|3end_saccharide_biosynthesis_regulatory_protein_944880..945030_POSITIVE 288 120 72 141 76 aact:AA02723|3end_hypothetical_protein_1906453..1906603_POSITIVE