Origin IGS: tgatattttaaaattatcctgcccacctgagcttacaggagaaggtatacaaaggatagaaaggaggtaatacgttccccagtcattttattttaccattaccctttatcttccgaagctccatattcccataatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgattatgggaatatggagcttcggaagataaagggtaatggtaaaataaaatgactggggaacgtattacctcctttctatcctttgtataccttctcctgtaagctcaggtgggcaggataattttaaaatatca Mask Tandem Repeat Region ================================================ tgatattttaaaattatcctgcccacctgagcttacaggagaaggtatacaaaggatagaaaggaggtaatacgttccccagtcattttattttaccattaccctttatcttccgaagctccatattcccataatca Find is-nt database================================================ Query_seq: AA01271:AA01272|AA01271:AA01272:hypothetical protein:hypothetical protein:->->:864042..864178 137 tgatattttaaaattatcctgcccacctgagcttacaggagaaggtatacaaaggatagaaaggaggtaatacgttccccagtcattttattttaccattaccctttatcttccgaagctccatattcccataatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01271:AA01272|AA01271:AA01272:hypothetical protein:hypothetical protein:->->:864042..864178 137 tgatattttaaaattatcctgcccacctgagcttacaggagaaggtatacaaaggatagaaaggaggtaatacgttccccagtcattttattttaccattaccctttatcttccgaagctccatattcccataatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01271:AA01272|AA01271:AA01272:hypothetical protein:hypothetical protein:->->:864042..864178 137 tgatattttaaaattatcctgcccacctgagcttacaggagaaggtatacaaaggatagaaaggaggtaatacgttccccagtcattttattttaccattaccctttatcttccgaagctccatattcccataatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgatattttaaaattatcctgcccacctgagcttacaggagaaggtatacaaaggatagaaaggaggtaatacgttccccagtcattttattttaccattaccctttatcttccgaagctccatattcccataatca Predict ORF larger than 30AA ================================================ tgatattttaaaattatcctgcccacctgagcttacaggagaaggtatacaaaggatagaaaggaggtaatacgttccccagtcattttattttaccattaccctttatcttccgaagctccatattcccataatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgatattttaaaattatcctgcccacctgagcttacaggagaaggtatacaaaggatagaaaggaggtaatacgttccccagtcattttattttaccattaccctttatcttccgaagctccatattcccataatca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01271:AA01272|AA01271:AA01272:hypothetical protein:hypothetical protein:->->:864042..864178 137 tgatattttaaaattatcctgcccacctgagcttacaggagaaggtatacaaaggatagaaaggaggtaatacgttccccagtcattttattttaccattaccctttatcttccgaagctccatattcccataatca Intra-Species Hit: Count: 1 Min: 1 Max: 137 Len: 137 Subject: aact_AA01271_AA01272|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[864042,864178]|137 HSP 1 e-value: 3.0E-72 bit: 272.0 Len: 137 Query Start:1 Query End:137 Subject Strand: POSITIVE Subject Start: 1 Subject End: 137 tgatattttaaaattatcctgcccacctgagcttacaggagaaggtatacaaaggatagaaaggaggtaatacgttccccagtcattttattttaccattaccctttatcttccgaagctccatattcccataatca tgatattttaaaattatcctgcccacctgagcttacaggagaaggtatacaaaggatagaaaggaggtaatacgttccccagtcattttattttaccattaccctttatcttccgaagctccatattcccataatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUAUUUUAAAAUUAUCCUGCCCACCUGAGCUUACAGGAGAAGGUAUACAAAGGAUAGAAAGGAGGUAAUACGUUCCCCAGUCAUUUUAUUUUACCAUUACCCUUUAUCUUCCGAAGCUCCAUAUUCCCAUAAUCA .((((.........))))..........((((((.(.(((((.((((.....(((((((((.((.((.........)))).....))))))))).....)))).....))))).)))))))................ (-20.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUUAUGGGAAUAUGGAGCUUCGGAAGAUAAAGGGUAAUGGUAAAAUAAAAUGACUGGGGAACGUAUUACCUCCUUUCUAUCCUUUGUAUACCUUCUCCUGUAAGCUCAGGUGGGCAGGAUAAUUUUAAAAUAUCA .(((...(((.(((..(((..(.(((((.....((((((((((...................)).)))))))).))))).)..)))..))).)))..((((((..((....))..))))))............))). (-28.31) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 284 116 73 98 49 aact:AA01996|5end_prephenate_dehydrogenase_1355314..1355544_POSITIVE 283 120 71 210 153 aact:AA01635|5end_ribonucleoside_diphosphate_reductase,_beta_chain_1104615..1104845_POSITIVE 267 56 19 43 1 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 256 45 11 87 57 aact:AA00816|5end_lacZ_expression_regulator_560900..561130_POSITIVE 249 55 17 222 177 aact:AA01475|5end_conserved_hypothetical_protein_(possible_cytoplasmic_protein;_DNA_binding_protein)_990364..990594_POSITIVE 249 55 17 117 72 aact:AA01474|5end_hypothetical_protein_990259..990489_POSITIVE 245 116 73 178 138 aact:AA03009|5end_hypothetical_protein_2101001..2101231_POSITIVE 245 60 12 207 158 aact:AA00900|5end_conserved_hypothetical_protein_623442..623672_POSITIVE 243 98 51 142 74 aact:AA02398|5end_DNA_primase_1649021..1649251_POSITIVE 243 56 25 102 73 aact:AA01029|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_D_719523..719753_POSITIVE 242 61 26 147 110 aact:AA00030|5end_hypothetical_protein_24879..25109_POSITIVE 241 46 18 85 47 aact:AA02772|5end_hypothetical_protein_1936244..1936474_POSITIVE 239 114 73 103 67 aact:AA01729|5end_hypothetical_protein_1168585..1168815_POSITIVE 237 78 37 84 39 aact:AA01549|5end_hypothetical_protein_1037444..1037674_POSITIVE 236 86 43 88 31 aact:AA02632|5end_rhamnosyltransferase_1831850..1832080_POSITIVE 236 130 82 191 135 aact:AA01721|5end_hypothetical_protein_1163312..1163542_POSITIVE 236 130 82 157 101 aact:AA01720|5end_conserved_hypothetical_protein_1163278..1163508_POSITIVE 235 56 13 140 81 aact:AA01695|5end_carbohydrate_transport_protein_1150445..1150675_POSITIVE 233 56 13 76 29 aact:AA01866|5end_thiamin_ABC_transporter,_permease_protein_1253313..1253543_POSITIVE 230 68 21 76 33 aact:AA00967|5end_conserved_hypothetical_protein_674546..674776_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 284 116 73 98 49 aact:AA01995|3end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355394..1355544_POSITIVE 260 52 11 138 95 aact:AA01881|3end_adenylate_kinase_1269765..1269915_POSITIVE 259 119 71 112 62 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 253 129 81 52 5 aact:AA02480|3end_excinuclease_ABC_subunit_B_1718442..1718592_POSITIVE 246 87 44 77 32 aact:AA01288|3end_hypothetical_protein_872288..872438_POSITIVE 243 56 25 95 66 aact:AA01028|3end_Na(+)-translocating_NADH-quinone_reductase_subunit_E_719596..719746_POSITIVE 242 61 26 131 94 aact:AA00029|3end_type_III_restriction-modification_system:_methylase_24943..25093_POSITIVE 239 114 73 103 67 aact:AA01728|3end_conserved_hypothetical_protein(related_to_HipA_protein)_1168665..1168815_POSITIVE 236 86 43 122 65 aact:AA02631|3end_undecaprenyl-phosphate_galactosephosphotransferase_homolog_1831964..1832114_POSITIVE 236 130 82 115 59 aact:AA01718|3end_DNA_polymerase_III,_chi_subunit_1163316..1163466_POSITIVE 235 56 13 82 23 aact:AA01694|3end_PTS_system,_IIA_component_1150467..1150617_POSITIVE 234 120 73 55 2 aact:AA01555|3end_ABC_transporter,_ATP-binding_protein_1044323..1044473_POSITIVE 230 55 18 97 48 aact:AA01474|3end_hypothetical_protein_990431..990581_POSITIVE 230 68 21 50 7 aact:AA00965|3end_hypothetical_protein_674600..674750_POSITIVE 228 55 13 97 40 aact:AA01273|3end_hypothetical_protein_864588..864738_POSITIVE 226 68 25 51 12 aact:AA02773|3end_hypothetical_protein_1936916..1937066_POSITIVE 225 53 20 52 16 aact:AA00882|3end_hypothetical_protein_613645..613795_POSITIVE 224 56 20 71 30 aact:AA02432|3end_penicillin-binding_protein_1B_1678325..1678475_POSITIVE 224 78 35 151 106 aact:AA01278|3end_hypothetical_protein_867725..867875_POSITIVE 223 67 21 77 40 aact:AA02605|3end_hypothetical_protein_1816760..1816910_POSITIVE