Origin IGS: acctatttcctcattttaatcgacgcgttaagcaaaagtgcggtcgaaatttattttgtttttgccgcagagagctcttccacgcgtttttcaagttcacttaatttttcacggatacgtatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatacgtatccgtgaaaaattaagtgaacttgaaaaacgcgtggaagagctctctgcggcaaaaacaaaataaatttcgaccgcacttttgcttaacgcgtcgattaaaatgaggaaataggt Mask Tandem Repeat Region ================================================ acctatttcctcattttaatcgacgcgttaagcaaaagtgcggtcgaaatttattttgtttttgccgcagagagctcttccacgcgtttttcaagttcacttaatttttcacggatacgtatta Find is-nt database================================================ Query_seq: AA01250:AA01251|AA01250:AA01251:nadAB transcriptional regulator:hypothetical protein:->->:851841..851964 124 acctatttcctcattttaatcgacgcgttaagcaaaagtgcggtcgaaatttattttgtttttgccgcagagagctcttccacgcgtttttcaagttcacttaatttttcacggatacgtatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01250:AA01251|AA01250:AA01251:nadAB transcriptional regulator:hypothetical protein:->->:851841..851964 124 acctatttcctcattttaatcgacgcgttaagcaaaagtgcggtcgaaatttattttgtttttgccgcagagagctcttccacgcgtttttcaagttcacttaatttttcacggatacgtatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01250:AA01251|AA01250:AA01251:nadAB transcriptional regulator:hypothetical protein:->->:851841..851964 124 acctatttcctcattttaatcgacgcgttaagcaaaagtgcggtcgaaatttattttgtttttgccgcagagagctcttccacgcgtttttcaagttcacttaatttttcacggatacgtatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 acctatttcctcattttaatcgacgcgttaagcaaaagtgcggtcgaaatttattttgtttttgccgcagagagctcttccacgcgtttttcaagttcacttaatttttcacggatacgtatta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 13 End: 123 ............ M L I D A L S K S A V E I Y F V F A A E S S S T R F S S S L N F S R I R I . acctatttcctcattttaatcgacgcgttaagcaaaagtgcggtcgaaatttattttgtttttgccgcagagagctcttccacgcgtttttcaagttcacttaatttttcacggatacgtatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ acctatttcctcattttaatcgacgcgttaagcaaaagtgcggtcgaaatttattttgtttttgccgcagagagctcttccacgcgtttttcaagttcacttaatttttcacggatacgtatta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01250:AA01251|AA01250:AA01251:nadAB transcriptional regulator:hypothetical protein:->->:851841..851964 124 acctatttcctcattttaatcgacgcgttaagcaaaagtgcggtcgaaatttattttgtttttgccgcagagagctcttccacgcgtttttcaagttcacttaatttttcacggatacgtatta Intra-Species Hit: Count: 1 Min: 1 Max: 124 Len: 124 Subject: aact_AA01250_AA01251|nadAB transcriptional regulator:hypothetical protein|POSITIVE:POSITIVE|[851841,851964]|124 HSP 1 e-value: 1.0E-64 bit: 246.0 Len: 124 Query Start:1 Query End:124 Subject Strand: POSITIVE Subject Start: 1 Subject End: 124 acctatttcctcattttaatcgacgcgttaagcaaaagtgcggtcgaaatttattttgtttttgccgcagagagctcttccacgcgtttttcaagttcacttaatttttcacggatacgtatta acctatttcctcattttaatcgacgcgttaagcaaaagtgcggtcgaaatttattttgtttttgccgcagagagctcttccacgcgtttttcaagttcacttaatttttcacggatacgtatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ ACCUAUUUCCUCAUUUUAAUCGACGCGUUAAGCAAAAGUGCGGUCGAAAUUUAUUUUGUUUUUGCCGCAGAGAGCUCUUCCACGCGUUUUUCAAGUUCACUUAAUUUUUCACGGAUACGUAUUA .......(((...........(((((((..(((.....((((((.(((((.......))))).))))))....))).....)))))))....(((....)))..........)))......... (-24.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUACGUAUCCGUGAAAAAUUAAGUGAACUUGAAAAACGCGUGGAAGAGCUCUCUGCGGCAAAAACAAAAUAAAUUUCGACCGCACUUUUGCUUAACGCGUCGAUUAAAAUGAGGAAAUAGGU .........(((........(((((....)))))...((((((....((((....((((((.(((..........))).).))))).....)))).))))))............)))....... (-25.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 462 69 21 136 85 aact:AA03010|5end_thiophene_and_furan_oxidation_protein_2101102..2101332_POSITIVE 428 69 24 152 109 aact:AA02993|5end_6-phosphofructokinase_2091766..2091996_POSITIVE 417 66 22 130 80 aact:AA00835|5end_UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapep_tide)_575212..575442_POSITIVE 417 69 35 154 118 aact:AA00759|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_I_520603..520833_POSITIVE 411 80 34 161 108 aact:AA02010|5end_peptide_methionine_sulfoxide_reductase;_cytochrome_C-TYPE_biogenesis_protein;_transcriptional_regulator_1367180..1367410_POSITIVE 408 69 36 36 1 aact:AA00758|5end_hypothetical_protein_520485..520715_POSITIVE 382 69 26 158 111 aact:AA00625|5end_hypothetical_protein_430565..430795_POSITIVE 382 69 26 50 3 aact:AA00623|5end_hypothetical_protein_430457..430687_POSITIVE 379 63 21 208 170 aact:AA01175|5end_6-phosphogluconate_dehydrogenase_804864..805094_POSITIVE 379 63 21 44 6 aact:AA01174|5end_hypothetical_protein_804700..804930_POSITIVE 375 69 21 202 139 aact:AA00912|5end_short-chain_dehydrogenase_627200..627430_POSITIVE 375 69 21 90 27 aact:AA00910|5end_hypothetical_protein_627088..627318_POSITIVE 374 80 31 164 107 aact:AA01407|5end_outer_membrane_antigenic_lipoprotein_b_precursor_949005..949235_POSITIVE 374 74 36 63 13 aact:AA00120|5end_ATP-dependent_Clp_protease_ATP-binding_subunit_88963..89193_POSITIVE 364 74 32 172 124 aact:AA01464|5end_hypothetical_protein_984210..984440_POSITIVE 363 69 25 148 96 aact:AA00124|5end_conserved_hypothetical_protein_(possible_cytoplasmic_protein)_90270..90500_POSITIVE 363 69 25 99 47 aact:AA00123|5end_hypothetical_protein_90221..90451_POSITIVE 363 69 25 90 38 aact:AA00122|5end_hypothetical_protein_90212..90442_POSITIVE 362 63 24 169 129 aact:AA01027|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_F_717676..717906_POSITIVE 356 79 34 69 22 aact:AA01877|5end_conserved_hypothetical_protein_1264115..1264345_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 428 69 24 46 3 aact:AA02992|3end_RNA_methyltransferase_2091740..2091890_POSITIVE 417 66 22 56 6 aact:AA00833|3end_UDP-N-acetylmuramate-alanine_ligase_575218..575368_POSITIVE 382 69 26 145 98 aact:AA00623|3end_hypothetical_protein_430632..430782_POSITIVE 376 69 30 74 32 aact:AA02781|3end_protein-export_membrane_protein_1945981..1946131_POSITIVE 375 79 31 69 21 aact:AA01804|3end_sugar_nucleotide_epimerase;_cell_division_inhibitor_1216001..1216151_POSITIVE 375 69 21 105 42 aact:AA00909|3end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_B_627183..627333_POSITIVE 365 74 37 50 1 aact:AA00119|3end_ATP-dependent_Clp_protease_proteolytic_subunit_(endopeptidase_Clp)_89030..89180_POSITIVE 364 74 32 82 34 aact:AA01463|3end_probable_transport_protein_984200..984350_POSITIVE 363 69 25 79 27 aact:AA00120|3end_ATP-dependent_Clp_protease_ATP-binding_subunit_90281..90431_POSITIVE 359 45 9 98 61 aact:AA00570|3end_hypothetical_protein_391785..391935_POSITIVE 355 65 21 88 42 aact:AA00345|3end_transcription_antitermination_protein_232058..232208_POSITIVE 354 68 21 86 33 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE 352 80 32 69 13 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 351 78 34 67 18 aact:AA02721|3end_spermidine/putrescine_ABC_transporter,_periplasmic-binding_protein_1905334..1905484_POSITIVE 351 69 31 76 37 aact:AA02338|3end_conserved_hypothetical_protein;_possible_integral_membrane_protein_1598885..1599035_POSITIVE 350 69 25 95 46 aact:AA02160|3end_conserved_hypothetical_protein_1478929..1479079_POSITIVE 350 68 25 51 4 aact:AA00894|3end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_621350..621500_POSITIVE 343 69 25 77 32 aact:AA02360|3end_threonine_synthase_1619947..1620097_POSITIVE 339 69 34 82 45 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 337 46 24 67 41 aact:AA00223|3end_catalase_(hydroperoxidase;_iron_heme_axial_ligand)_155808..155958_POSITIVE