Origin IGS: attgttaacatgttaaaacgaaaagaaaggtcatcttgcaataatcttcagaacatcaaactattattgagtatttaatagctttttgtgagtttaaaaagtatacatgtcggaaaatagggtggtgttttcacattttaaaaagttaatgtaaaaagctaaaggcaggatagcgatacgagcctaaaagtgatacaccttgctgaaatatatgttctgtgcgtaactagcaggctgtcaagaagtttgataaatgacaatatccacacagatttttaatagttgtttaaatca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgatttaaacaactattaaaaatctgtgtggatattgtcatttatcaaacttcttgacagcctgctagttacgcacagaacatatatttcagcaaggtgtatcacttttaggctcgtatcgctatcctgcctttagctttttacattaactttttaaaatgtgaaaacaccaccctattttccgacatgtatactttttaaactcacaaaaagctattaaatactcaataatagtttgatgttctgaagattattgcaagatgacctttcttttcgttttaacatgttaacaat Mask Tandem Repeat Region ================================================ attgttaacatgttaaaacgaaaagaaaggtcatcttgcaataatcttcagaacatcaaactattattgagtatttaatagctttttgtgagtttaaaaagtatacatgtcggaaaatagggtggtgttttcacattttaaaaagttaatgtaaaaagctaaaggcaggatagcgatacgagcctaaaagtgatacaccttgctgaaatatatgttctgtgcgtaactagcaggctgtcaagaagtttgataaatgacaatatccacacagatttttaatagttgtttaaatca Find is-nt database================================================ Query_seq: AA01176:AA01177|AA01176:AA01177:hypothetical protein:beta-ketoacyl-ACP synthase IV:->->:806578..806871 294 attgttaacatgttaaaacgaaaagaaaggtcatcttgcaataatcttcagaacatcaaactattattgagtatttaatagctttttgtgagtttaaaaagtatacatgtcggaaaatagggtggtgttttcacattttaaaaagttaatgtaaaaagctaaaggcaggatagcgatacgagcctaaaagtgatacaccttgctgaaatatatgttctgtgcgtaactagcaggctgtcaagaagtttgataaatgacaatatccacacagatttttaatagttgtttaaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01176:AA01177|AA01176:AA01177:hypothetical protein:beta-ketoacyl-ACP synthase IV:->->:806578..806871 294 attgttaacatgttaaaacgaaaagaaaggtcatcttgcaataatcttcagaacatcaaactattattgagtatttaatagctttttgtgagtttaaaaagtatacatgtcggaaaatagggtggtgttttcacattttaaaaagttaatgtaaaaagctaaaggcaggatagcgatacgagcctaaaagtgatacaccttgctgaaatatatgttctgtgcgtaactagcaggctgtcaagaagtttgataaatgacaatatccacacagatttttaatagttgtttaaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01176:AA01177|AA01176:AA01177:hypothetical protein:beta-ketoacyl-ACP synthase IV:->->:806578..806871 294 attgttaacatgttaaaacgaaaagaaaggtcatcttgcaataatcttcagaacatcaaactattattgagtatttaatagctttttgtgagtttaaaaagtatacatgtcggaaaatagggtggtgttttcacattttaaaaagttaatgtaaaaagctaaaggcaggatagcgatacgagcctaaaagtgatacaccttgctgaaatatatgttctgtgcgtaactagcaggctgtcaagaagtttgataaatgacaatatccacacagatttttaatagttgtttaaatca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 attgttaacatgttaaaacgaaaagaaaggtcatcttgcaataatcttcagaacatcaaactattattgagtatttaatagctttttgtgagtttaaaaagtatacatgtcggaaaatagggtggtgttttcacattttaaaaagttaatgtaaaaagctaaaggcaggatagcgatacgagcctaaaagtgatacaccttgctgaaatatatgttctgtgcgtaactagcaggctgtcaagaagtttgataaatgacaatatccacacagatttttaatagttgtttaaatca Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: - Start: 162 End: 269 ................................................................................................................................................................. L C S L S V L R F T I C R A S I Y T R H T V L L S D L L K I F S L I W M ......................... attgttaacatgttaaaacgaaaagaaaggtcatcttgcaataatcttcagaacatcaaactattattgagtatttaatagctttttgtgagtttaaaaagtatacatgtcggaaaatagggtggtgttttcacattttaaaaagttaatgtaaaaagctaaaggcaggatagcgatacgagcctaaaagtgatacaccttgctgaaatatatgttctgtgcgtaactagcaggctgtcaagaagtttgataaatgacaatatccacacagatttttaatagttgtttaaatca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ attgttaacatgttaaaacgaaaagaaaggtcatcttgcaataatcttcagaacatcaaactattattgagtatttaatagctttttgtgagtttaaaaagtatacatgtcggaaaatagggtggtgttttcacattttaaaaagttaatgtaaaaagctaaaggcaggatagcgatacgagcctaaaagtgatacaccttgctgaaatatatgttctgtgcgtaactagcaggctgtcaagaagtttgataaatgacaatatccacacagatttttaatagttgtttaaatca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01176:AA01177|AA01176:AA01177:hypothetical protein:beta-ketoacyl-ACP synthase IV:->->:806578..806871 294 attgttaacatgttaaaacgaaaagaaaggtcatcttgcaataatcttcagaacatcaaactattattgagtatttaatagctttttgtgagtttaaaaagtatacatgtcggaaaatagggtggtgttttcacattttaaaaagttaatgtaaaaagctaaaggcaggatagcgatacgagcctaaaagtgatacaccttgctgaaatatatgttctgtgcgtaactagcaggctgtcaagaagtttgataaatgacaatatccacacagatttttaatagttgtttaaatca Intra-Species Hit: Count: 1 Min: 1 Max: 294 Len: 294 Subject: aact_AA01176_AA01177|hypothetical protein:beta-ketoacyl-ACP synthase IV|POSITIVE:POSITIVE|[806578,806871]|294 HSP 1 e-value: 1.0E-165 bit: 583.0 Len: 294 Query Start:1 Query End:294 Subject Strand: POSITIVE Subject Start: 1 Subject End: 294 attgttaacatgttaaaacgaaaagaaaggtcatcttgcaataatcttcagaacatcaaactattattgagtatttaatagctttttgtgagtttaaaaagtatacatgtcggaaaatagggtggtgttttcacattttaaaaagttaatgtaaaaagctaaaggcaggatagcgatacgagcctaaaagtgatacaccttgctgaaatatatgttctgtgcgtaactagcaggctgtcaagaagtttgataaatgacaatatccacacagatttttaatagttgtttaaatca attgttaacatgttaaaacgaaaagaaaggtcatcttgcaataatcttcagaacatcaaactattattgagtatttaatagctttttgtgagtttaaaaagtatacatgtcggaaaatagggtggtgttttcacattttaaaaagttaatgtaaaaagctaaaggcaggatagcgatacgagcctaaaagtgatacaccttgctgaaatatatgttctgtgcgtaactagcaggctgtcaagaagtttgataaatgacaatatccacacagatttttaatagttgtttaaatca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUGUUAACAUGUUAAAACGAAAAGAAAGGUCAUCUUGCAAUAAUCUUCAGAACAUCAAACUAUUAUUGAGUAUUUAAUAGCUUUUUGUGAGUUUAAAAAGUAUACAUGUCGGAAAAUAGGGUGGUGUUUUCACAUUUUAAAAAGUUAAUGUAAAAAGCUAAAGGCAGGAUAGCGAUACGAGCCUAAAAGUGAUACACCUUGCUGAAAUAUAUGUUCUGUGCGUAACUAGCAGGCUGUCAAGAAGUUUGAUAAAUGACAAUAUCCACACAGAUUUUUAAUAGUUGUUUAAAUCA (((((((......................(..(((((((..........(((((((((..(((((.((((((((......((((((((......))))))))))))...))))..)))))..))))))))).(((((..........)))))............)))))))..)....(((((.......(((((...(((((((.....(((((.....)))))..))))))).)))))....))))).....))))))).........(((((..(((....))).))))). (-53.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAUUUAAACAACUAUUAAAAAUCUGUGUGGAUAUUGUCAUUUAUCAAACUUCUUGACAGCCUGCUAGUUACGCACAGAACAUAUAUUUCAGCAAGGUGUAUCACUUUUAGGCUCGUAUCGCUAUCCUGCCUUUAGCUUUUUACAUUAACUUUUUAAAAUGUGAAAACACCACCCUAUUUUCCGACAUGUAUACUUUUUAAACUCACAAAAAGCUAUUAAAUACUCAAUAAUAGUUUGAUGUUCUGAAGAUUAUUGCAAGAUGACCUUUCUUUUCGUUUUAACAUGUUAACAAU ......................((((((((...((((.((....((((.....)))).....)).))))..))))))))................(((((....((...((((..(((....)))...))))..))..(((((((((..........))))))))))))))............(((((((...............(((..(((((((((.........)))))))))..)))...((((.(((((....))))).))))............)))))))...... (-47.60) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 326 239 192 208 154 aact:AA00844|5end_cell_division_protein_I;_penicillin_binding_protein_2X_582802..583032_POSITIVE 314 203 168 42 7 aact:AA01195|5end_acyl_carrier_protein_819849..820079_POSITIVE 309 234 197 59 28 aact:AA02252|5end_conserved_hypothetical_protein_1533840..1534070_POSITIVE 303 238 198 166 125 aact:AA02141|5end_ABC_transporter,_permease_protein_1464890..1465120_POSITIVE 286 240 191 58 14 aact:AA02532|5end_conserved_hypothetical_protein_1759566..1759796_POSITIVE 282 199 158 46 5 aact:AA02810|5end_glycine_hydroxymethyltransferase;_serine_hydroxymethyltransferase_1978185..1978415_POSITIVE 280 224 188 182 137 aact:AA01651|5end_hypothetical_protein_1119796..1120026_POSITIVE 275 207 164 201 155 aact:AA02736|5end_conserved_hypothetical_protein_1917350..1917580_POSITIVE 275 207 164 223 169 aact:AA02565|5end_transketolase,_N-terminal_subunit_1786366..1786596_POSITIVE 275 200 156 117 78 aact:AA01584|5end_host_factor-I_protein_1065589..1065819_POSITIVE 273 242 219 54 28 aact:AA00544|5end_glutathionylspermidine_synthetase;amidase_372448..372678_POSITIVE 270 210 161 220 155 aact:AA01615|5end_lipoprotein_releasing_system_transmembrane_1091186..1091416_POSITIVE 267 255 212 195 152 aact:AA02283|5end_possible_phosphoglycerate_transport_system_activator_protein_1553782..1554012_POSITIVE 265 206 164 99 33 aact:AA00990|5end_conserved_hypothetical_protein_692559..692789_POSITIVE 264 234 196 80 49 aact:AA02536|5end_conserved_hypothetical_protein_1764854..1765084_POSITIVE 264 197 156 152 113 aact:AA01807|5end_electron_transport_complex_protein_1216696..1216926_POSITIVE 264 209 164 213 170 aact:AA00461|5end_heat_shock_protein;_protease_322375..322605_POSITIVE 263 240 203 207 166 aact:AA01817|5end_hypothetical_protein_1224225..1224455_POSITIVE 262 222 182 119 88 aact:AA01256|5end_hypothetical_protein_852774..853004_POSITIVE 260 250 210 217 178 aact:AA02836|5end_colicin_transport_protein_1991777..1992007_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 314 203 168 36 1 aact:AA01194|3end_acyl_carrier_protein_819923..820073_POSITIVE 276 219 173 151 93 aact:AA01650|3end_maltose_transport_inner_membrane_protein_1119849..1119999_POSITIVE 275 200 156 99 60 aact:AA01582|3end_hypothetical_protein_1065651..1065801_POSITIVE 265 206 164 104 38 aact:AA00989|3end_hypothetical_protein_692644..692794_POSITIVE 264 197 156 149 110 aact:AA01805|3end_electron_transport_complex_protein_1216773..1216923_POSITIVE 264 205 167 124 84 aact:AA00088|3end_30S_ribosomal_protein_S15_66387..66537_POSITIVE 262 199 162 80 46 aact:AA01875|3end_PTS_system,_glucose-specific_enzyme_II,_A_component_1263506..1263656_POSITIVE 262 222 182 138 107 aact:AA01255|3end_hypothetical_protein_852873..853023_POSITIVE 260 250 210 128 89 aact:AA02834|3end_colicin_transport_protein_1991768..1991918_POSITIVE 260 204 164 111 69 aact:AA02440|3end_ABC_transporter,_ATP-binding_protein_1685791..1685941_POSITIVE 254 237 196 141 93 aact:AA02139|3end_possible_membrane_protein_1462218..1462368_POSITIVE 251 192 163 53 21 aact:AA01751|3end_conserved_hypothetical_protein_1185392..1185542_POSITIVE 249 203 164 40 12 aact:AA01087|3end_DNA_polymerase_III,_delta_subunit_752980..753130_POSITIVE 247 246 217 127 89 aact:AA02927|3end_hypothetical_protein_2048405..2048555_POSITIVE 247 246 217 69 31 aact:AA02926|3end_hypothetical_protein_2048347..2048497_POSITIVE 245 199 151 55 9 aact:AA02678|3end_formate_dehydrogenase,_gamma_subunit_1865523..1865673_POSITIVE 244 240 211 36 5 aact:AA01816|3end_hypothetical_protein_1224134..1224284_POSITIVE 242 226 182 56 12 aact:AA02563|3end_transketolase_C-terminal_section_1786260..1786410_POSITIVE 242 204 163 130 78 aact:AA02158|3end_TonB-dependent_outer_membrane_receptor_1477317..1477467_POSITIVE 241 207 167 147 110 aact:AA01651|3end_hypothetical_protein_1120004..1120154_POSITIVE