Origin IGS: taaataattatgctgatcttttttattctggtgatgattctattaaatggcatgagaaagagagtgcaacagacagggataataacaaaatggctaaggagttattttctactttgaaaaagcttttaattaaataatcgtgtagatggaacttatcctcccttgctgacgcgcgccggtatgaataaaaacgtttaaaaaaatgaccgcacttttattcgatatgcaaatggtaaatcagagaaaataac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gttattttctctgatttaccatttgcatatcgaataaaagtgcggtcatttttttaaacgtttttattcataccggcgcgcgtcagcaagggaggataagttccatctacacgattatttaattaaaagctttttcaaagtagaaaataactccttagccattttgttattatccctgtctgttgcactctctttctcatgccatttaatagaatcatcaccagaataaaaaagatcagcataattattta Mask Tandem Repeat Region ================================================ taaataattatgctgatcttttttattctggtgatgattctattaaatggcatgagaaagagagtgcaacagacagggataataacaaaatggctaaggagttattttctactttgaaaaagcttttaattaaataatcgtgtagatggaacttatcctcccttgctgacgcgcgccggtatgaataaaaacgtttaaaaaaatgaccgcacttttattcgatatgcaaatggtaaatcagagaaaataac Find is-nt database================================================ Query_seq: AA01170:AA01171|AA01170:AA01171:hypothetical protein:LysA activator protein:->->:802700..802950 251 taaataattatgctgatcttttttattctggtgatgattctattaaatggcatgagaaagagagtgcaacagacagggataataacaaaatggctaaggagttattttctactttgaaaaagcttttaattaaataatcgtgtagatggaacttatcctcccttgctgacgcgcgccggtatgaataaaaacgtttaaaaaaatgaccgcacttttattcgatatgcaaatggtaaatcagagaaaataac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01170:AA01171|AA01170:AA01171:hypothetical protein:LysA activator protein:->->:802700..802950 251 taaataattatgctgatcttttttattctggtgatgattctattaaatggcatgagaaagagagtgcaacagacagggataataacaaaatggctaaggagttattttctactttgaaaaagcttttaattaaataatcgtgtagatggaacttatcctcccttgctgacgcgcgccggtatgaataaaaacgtttaaaaaaatgaccgcacttttattcgatatgcaaatggtaaatcagagaaaataac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01170:AA01171|AA01170:AA01171:hypothetical protein:LysA activator protein:->->:802700..802950 251 taaataattatgctgatcttttttattctggtgatgattctattaaatggcatgagaaagagagtgcaacagacagggataataacaaaatggctaaggagttattttctactttgaaaaagcttttaattaaataatcgtgtagatggaacttatcctcccttgctgacgcgcgccggtatgaataaaaacgtttaaaaaaatgaccgcacttttattcgatatgcaaatggtaaatcagagaaaataac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaataattatgctgatcttttttattctggtgatgattctattaaatggcatgagaaagagagtgcaacagacagggataataacaaaatggctaaggagttattttctactttgaaaaagcttttaattaaataatcgtgtagatggaacttatcctcccttgctgacgcgcgccggtatgaataaaaacgtttaaaaaaatgaccgcacttttattcgatatgcaaatggtaaatcagagaaaataac Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 4 End: 114 ... M I M L I F F I L V M I L L N G M R K R V Q Q T G I I T K W L R S Y F L L ......................................................................................................................................... Protein_Len: 31 Strand: + Start: 42 End: 134 ......................................... M K W H E K E S A T D R D N N K M A K E L F S T L K K L L I K ..................................................................................................................... Protein_Len: 33 Strand: - Start: 133 End: 231 .................................................................................................................................... I I T Y I S S I R G Q Q R A G T H I F V N L F I V A S K N S I C M .................... Protein_Len: 35 Strand: - Start: 26 End: 130 ......................... E P S S E I L H C S F S L A V S L S L L L I A L S N N E V K F F S K M ......................................................................................................................... taaataattatgctgatcttttttattctggtgatgattctattaaatggcatgagaaagagagtgcaacagacagggataataacaaaatggctaaggagttattttctactttgaaaaagcttttaattaaataatcgtgtagatggaacttatcctcccttgctgacgcgcgccggtatgaataaaaacgtttaaaaaaatgaccgcacttttattcgatatgcaaatggtaaatcagagaaaataac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaataattatgctgatcttttttattctggtgatgattctattaaatggcatgagaaagagagtgcaacagacagggataataacaaaatggctaaggagttattttctactttgaaaaagcttttaattaaataatcgtgtagatggaacttatcctcccttgctgacgcgcgccggtatgaataaaaacgtttaaaaaaatgaccgcacttttattcgatatgcaaatggtaaatcagagaaaataac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01170:AA01171|AA01170:AA01171:hypothetical protein:LysA activator protein:->->:802700..802950 251 taaataattatgctgatcttttttattctggtgatgattctattaaatggcatgagaaagagagtgcaacagacagggataataacaaaatggctaaggagttattttctactttgaaaaagcttttaattaaataatcgtgtagatggaacttatcctcccttgctgacgcgcgccggtatgaataaaaacgtttaaaaaaatgaccgcacttttattcgatatgcaaatggtaaatcagagaaaataac Intra-Species Hit: Count: 1 Min: 1 Max: 251 Len: 251 Subject: aact_AA01170_AA01171|hypothetical protein:LysA activator protein|POSITIVE:POSITIVE|[802700,802950]|251 HSP 1 e-value: 1.0E-127 bit: 456.0 Len: 251 Query Start:1 Query End:251 Subject Strand: POSITIVE Subject Start: 1 Subject End: 251 taaataattatgctgatcttttttattctggtgatgattctattaaatggcatgagaaagagagtgcaacagacagggataataacaaaatggctaaggagttattttctactttgaaaaagcttttaattaaataatcgtgtagatggaacttatcctcccttgctgacgcgcgccggtatgaataaaaacgtttnnnnnnntgaccgcacttttattcgatatgcaaatggtaaatcagagaaaataac taaataattatgctgatcttttttattctggtgatgattctattaaatggcatgagaaagagagtgcaacagacagggataataacaaaatggctaaggagttattttctactttgaaaaagcttttaattaaataatcgtgtagatggaacttatcctcccttgctgacgcgcgccggtatgaataaaaacgtttaaaaaaatgaccgcacttttattcgatatgcaaatggtaaatcagagaaaataac Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAAUUAUGCUGAUCUUUUUUAUUCUGGUGAUGAUUCUAUUAAAUGGCAUGAGAAAGAGAGUGCAACAGACAGGGAUAAUAACAAAAUGGCUAAGGAGUUAUUUUCUACUUUGAAAAAGCUUUUAAUUAAAUAAUCGUGUAGAUGGAACUUAUCCUCCCUUGCUGACGCGCGCCGGUAUGAAUAAAAACGUUUAAAAAAAUGACCGCACUUUUAUUCGAUAUGCAAAUGGUAAAUCAGAGAAAAUAAC .......((((((((.((((((((((.((..(((((....)))))...)).))))))))))..((((..(((..(((((..((((...........(((((((.(((((......)))))))))))).........(((.....))).....))))..)))))..)))..))))..)))))))).......(((((....)))))............((((((.(((.....))).))).)))........ (-48.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUAUUUUCUCUGAUUUACCAUUUGCAUAUCGAAUAAAAGUGCGGUCAUUUUUUUAAACGUUUUUAUUCAUACCGGCGCGCGUCAGCAAGGGAGGAUAAGUUCCAUCUACACGAUUAUUUAAUUAAAAGCUUUUUCAAAGUAGAAAAUAACUCCUUAGCCAUUUUGUUAUUAUCCCUGUCUGUUGCACUCUCUUUCUCAUGCCAUUUAAUAGAAUCAUCACCAGAAUAAAAAAGAUCAGCAUAAUUAUUUA ...............................((((((..((((((((.(((....)))..(((((((((.....((((.(((.(((..((((((((.....))).(((((..((....................))...))))).((((((..............)))))).)))))..))).))).)............)))........((....))....))))))))).)))).))))..)))))). (-36.69) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 450 215 174 125 84 aact:AA01832|5end_formate_dehydrogenase_formation_protein_E_1233609..1233839_POSITIVE 439 215 171 162 114 aact:AA00130|5end_mercuric_transport_protein_(periplasmic_mercury_ion_binding_protein)_(mercury_scavenger_protein)_94217..94447_POSITIVE 439 215 171 67 19 aact:AA00129|5end_hypothetical_protein_94122..94352_POSITIVE 420 217 171 175 126 aact:AA02504|5end_lipid_A_biosynthesis_(kdo)2-(lauroyl)-lipid_IVA_acyltransferase_1738215..1738445_POSITIVE 407 215 171 124 77 aact:AA02716|5end_peptidase_T_1899789..1900019_POSITIVE 407 218 174 124 73 aact:AA01936|5end_tyrosyl-tRNA_synthetase_1307043..1307273_POSITIVE 406 219 171 127 69 aact:AA01994|5end_chorismate_mutase;_prephenate_dehydrogenase_1354959..1355189_POSITIVE 402 216 171 177 137 aact:AA00212|5end_ribokinase_146056..146286_POSITIVE 402 216 171 76 36 aact:AA00211|5end_hypothetical_protein_145955..146185_POSITIVE 401 217 171 110 64 aact:AA01220|5end_competence_protein_F;_amidophosphoribosyltransferase_832775..833005_POSITIVE 390 218 171 125 82 aact:AA01393|5end_ABC_transporter,_ATP-binding_protein_940369..940599_POSITIVE 390 210 163 82 32 aact:AA00286|5end_glutamate_5-kinase_(gamma-glutamyl_kinase)_199965..200195_POSITIVE 387 219 171 85 30 aact:AA01969|5end_hypothetical_protein_1331969..1332199_POSITIVE 371 216 171 123 62 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 371 219 172 67 27 aact:AA00349|5end_hypothetical_protein_233274..233504_POSITIVE 368 215 171 132 83 aact:AA01972|5end_phosphotransacetylase_1332219..1332449_POSITIVE 362 228 181 90 44 aact:AA02965|5end_hypothetical_protein_2074069..2074299_POSITIVE 361 180 141 176 137 aact:AA00790|5end_recombination_DNA_repair_protein_542455..542685_POSITIVE 357 217 172 52 9 aact:AA02846|5end_crossover_junction_endodeoxyribonuclease_1998648..1998878_POSITIVE 356 216 173 221 180 aact:AA02778|5end_hypothetical_protein_1942585..1942815_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 450 215 174 45 4 aact:AA01830|3end_2,3-cyclic-nucleotide_2-phosphodiesterase_1233609..1233759_POSITIVE 439 215 171 72 24 aact:AA00128|3end_cation-transporting_ATPase_(probable_potassium/copper_transporter)_94207..94357_POSITIVE 420 217 171 150 101 aact:AA02502|3end_adenine_phosphoribosyltransferase_1738270..1738420_POSITIVE 406 219 171 92 34 aact:AA01992|3end_protease_IV;_signal_peptide_peptidase;_Endopeptidase_IV_1355004..1355154_POSITIVE 402 216 171 73 33 aact:AA00209|3end_D-ribose_ABC_transporter,_periplasmic-binding_protein_146032..146182_POSITIVE 401 217 171 110 64 aact:AA01217|3end_hypothetical_protein_832855..833005_POSITIVE 390 218 171 78 35 aact:AA01391|3end_glycerol-3-phosphate_acyltransferase_940402..940552_POSITIVE 390 210 163 52 2 aact:AA00283|3end_glutamate_5-kinase_(gamma-glutamyl_kinase)_200015..200165_POSITIVE 371 219 172 49 9 aact:AA00348|3end_50S_ribosomal_protein_L1_233336..233486_POSITIVE 367 215 173 42 4 aact:AA02715|3end_paraquat-inducible_protein_pqiB_homolog_1899787..1899937_POSITIVE 362 228 181 69 23 aact:AA02964|3end_opacity_associated_protein_A_2074128..2074278_POSITIVE 356 216 173 83 42 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 347 215 171 149 100 aact:AA01025|3end_hypothetical_protein_717769..717919_POSITIVE 337 219 174 126 81 aact:AA01044|3end_A/G-specific_adenine_glycosylase_727978..728128_POSITIVE 337 215 173 42 2 aact:AA00764|3end_hemoglobin_binding_protein_A_527347..527497_POSITIVE 336 233 191 63 14 aact:AA00237|3end_hypothetical_protein_171169..171319_POSITIVE 335 215 173 89 49 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 335 214 172 59 14 aact:AA00112|3end_uracil_permease_81239..81389_POSITIVE 334 227 185 94 48 aact:AA00578|3end_DNA_topoisomerase_395201..395351_POSITIVE 333 237 191 104 64 aact:AA01434|3end_conserved_hypothetical_protein_(possible_o-antigen_ligase)_963021..963171_POSITIVE