Origin IGS: tagttggaatcaatataaggaatattcactgatagcgcacgcttcatagcgtgtgcttgtagataaaggcacacgttgggaaacatgcgccatcgtaggaaactattggaggaattacatgaaacataacttatttattttatatggtgcggctaatacaggtaaaaccacaacttttaatgagttactaaaaaatgcttgtgatcaattcttagataaattggtgtattttgaacggagtgaaaattgtgctgactttttagctgtctttcaaaataaaaatgtgaaagtagacctatattcttcaggcgata .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tatcgcctgaagaatataggtctactttcacatttttattttgaaagacagctaaaaagtcagcacaattttcactccgttcaaaatacaccaatttatctaagaattgatcacaagcattttttagtaactcattaaaagttgtggttttacctgtattagccgcaccatataaaataaataagttatgtttcatgtaattcctccaatagtttcctacgatggcgcatgtttcccaacgtgtgcctttatctacaagcacacgctatgaagcgtgcgctatcagtgaatattccttatattgattccaacta Mask Tandem Repeat Region ================================================ tagttggaatcaatataaggaatattcactgatagcgcacgcttcatagcgtgtgcttgtagataaaggcacacgttgggaaacatgcgccatcgtaggaaactattggaggaattacatgaaacataacttatttattttatatggtgcggctaatacaggtaaaaccacaacttttaatgagttactaaaaaatgcttgtgatcaattcttagataaattggtgtattttgaacggagtgaaaattgtgctgactttttagctgtctttcaaaataaaaatgtgaaagtagacctatattcttcaggcgata Find is-nt database================================================ Query_seq: AA01169:AA01170|AA01169:AA01170:hypothetical protein:hypothetical protein:->->:802290..802603 314 tagttggaatcaatataaggaatattcactgatagcgcacgcttcatagcgtgtgcttgtagataaaggcacacgttgggaaacatgcgccatcgtaggaaactattggaggaattacatgaaacataacttatttattttatatggtgcggctaatacaggtaaaaccacaacttttaatgagttactaaaaaatgcttgtgatcaattcttagataaattggtgtattttgaacggagtgaaaattgtgctgactttttagctgtctttcaaaataaaaatgtgaaagtagacctatattcttcaggcgata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01169:AA01170|AA01169:AA01170:hypothetical protein:hypothetical protein:->->:802290..802603 314 tagttggaatcaatataaggaatattcactgatagcgcacgcttcatagcgtgtgcttgtagataaaggcacacgttgggaaacatgcgccatcgtaggaaactattggaggaattacatgaaacataacttatttattttatatggtgcggctaatacaggtaaaaccacaacttttaatgagttactaaaaaatgcttgtgatcaattcttagataaattggtgtattttgaacggagtgaaaattgtgctgactttttagctgtctttcaaaataaaaatgtgaaagtagacctatattcttcaggcgata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01169:AA01170|AA01169:AA01170:hypothetical protein:hypothetical protein:->->:802290..802603 314 tagttggaatcaatataaggaatattcactgatagcgcacgcttcatagcgtgtgcttgtagataaaggcacacgttgggaaacatgcgccatcgtaggaaactattggaggaattacatgaaacataacttatttattttatatggtgcggctaatacaggtaaaaccacaacttttaatgagttactaaaaaatgcttgtgatcaattcttagataaattggtgtattttgaacggagtgaaaattgtgctgactttttagctgtctttcaaaataaaaatgtgaaagtagacctatattcttcaggcgata Intra-Species Hit: Count: 0 Inter-species Hit: Count: 2 Min: 131 Max: 313 Len: 183 Subject: UniRef90_A3N1V6 Cluster: Hypothetical protein; n=2; Actinobacillus pleuropneumoniae|Rep: Hypothetical protein - Actinobacillus pleuropneumoniae serotype 5b (strain L20) HSP 1 e-value: 3.0E-15 bit: 83.2 Len: 183 Query Start:131 Query End:313 Subject Strand: null Subject Start: 4 Subject End: 64 .................................................................................................................................. L F I L Y G A A N T G K T T T F N E L L K N A C D Q F L D K L V Y F E R S E N C A D F L A V F Q N K N V K V D L Y S S G D . .................................................................................................................................. L F I L Y G A G N T G K T T T F N K L L E K I N A K Y L D K L V Y F S R H S N N I D F I A V F Q Y E N M R I G F Y S S G D . Subject: UniRef90_A1KRS8 Cluster: Hypothetical protein; n=3; Neisseria meningitidis|Rep: Hypothetical protein - Neisseria meningitidis serogroup C / serotype 2a (strain ATCC 700532 /FAM18) HSP 1 e-value: 1.0E-10 bit: 67.8 Len: 183 Query Start:131 Query End:313 Subject Strand: null Subject Start: 6 Subject End: 66 .................................................................................................................................. L F I L Y G A A N T G K T T T F N E L L K N A C D Q F L D K L V Y F E R S E N C A D F L A V F Q N K N V K V D L Y S S G D . .................................................................................................................................. I F I L Y G A A N K G K S T T L N T L F N Q I C R K F S K F L V F F E R H G N G L D F V A V F D H E G Q R I G F Y S S G D . tagttggaatcaatataaggaatattcactgatagcgcacgcttcatagcgtgtgcttgtagataaaggcacacgttgggaaacatgcgccatcgtaggaaactattggaggaattacatgaaacataacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna Predict ORF larger than 30AA ================================================ Protein_Len: 60 Strand: + Start: 53 End: 313 .................................................... M L V D K G T R W E T C A I V G N Y W R N Y M K H N L F I L Y G A A N T G K T T T F N E L L K N A C D Q F L D K L V Y F . Protein_Len: 43 Strand: - Start: 180 End: 308 ................................................................................................................................................................................... H T V L F H K H D I R L Y I P T N Q V S H F N H Q S K L Q R E F Y F H S L L G I N K M ...... Protein_Len: 44 Strand: - Start: 3 End: 134 .. N S D I Y P I N V S L A R K M A H A Q L Y L C V N P F M R W R L F S N S S N C S V Y S M .................................................................................................................................................................................... tagttggaatcaatataaggaatattcactgatagcgcacgcttcatagcgtgtgcttgtagataaaggcacacgttgggaaacatgcgccatcgtaggaaactattggaggaattacatgaaacataacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagttggaatcaatataaggaatattcactgatagcgcacgcttcatagcgtgtgcttgtagataaaggcacacgttgggaaacatgcgccatcgtaggaaactattggaggaattacatgaaacataacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 87 HP_score: -9.6 Tail_Score: -4.03234 Start: 68 End: 91 Full_Region: atagtttcctacgat ggcgcatgtt tccc aacgtgtgcc tttatctacaagcac ...................................................................ggcgcatgtttcccaacgtgtgcc............................................................................................................................................................................................................................... Find igs database================================================ Query_seq: AA01169:AA01170|AA01169:AA01170:hypothetical protein:hypothetical protein:->->:802290..802603 314 tagttggaatcaatataaggaatattcactgatagcgcacgcttcatagcgtgtgcttgtagataaaggcacacgttgggaaacatgcgccatcgtaggaaactattggaggaattacatgaaacataacnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna Intra-Species Hit: Count: 1 Min: 1 Max: 130 Len: 130 Subject: aact_AA01169_AA01170|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[802290,802603]|314 HSP 1 e-value: 1.0E-67 bit: 258.0 Len: 130 Query Start:1 Query End:130 Subject Strand: POSITIVE Subject Start: 1 Subject End: 130 tagttggaatcaatataaggaatattcactgatagcgcacgcttcatagcgtgtgcttgtagataaaggcacacgttgggaaacatgcgccatcgtaggaaactattggaggaattacatgaaacataac........................................................................................................................................................................................ tagttggaatcaatataaggaatattcactgatagcgcacgcttcatagcgtgtgcttgtagataaaggcacacgttgggaaacatgcgccatcgtaggaaactattggaggaattacatgaaacataac........................................................................................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUGGAAUCAAUAUAAGGAAUAUUCACUGAUAGCGCACGCUUCAUAGCGUGUGCUUGUAGAUAAAGGCACACGUUGGGAAACAUGCGCCAUCGUAGGAAACUAUUGGAGGAAUUACAUGAAACAUAAC ..((((....))))..........((((..(((.(((((.(.(((.((((((((((((........)))))))))))).))).).))))).))).(((....)))..............))))....... (-37.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUAUGUUUCAUGUAAUUCCUCCAAUAGUUUCCUACGAUGGCGCAUGUUUCCCAACGUGUGCCUUUAUCUACAAGCACACGCUAUGAAGCGUGCGCUAUCAGUGAAUAUUCCUUAUAUUGAUUCCAACUA .........................(((((......(((((((((((((((....(((((((............)))))))....))))))))))))))).((.(((((.....))))).))...))))) (-38.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 495 90 43 171 114 aact:AA02450|5end_conserved_hypothetical_protein_1687701..1687931_POSITIVE 395 90 41 144 82 aact:AA01770|5end_hypothetical_protein_1193111..1193341_POSITIVE 387 96 51 180 136 aact:AA02212|5end_ribonucleoside-diphosphate_reductase_beta_chain_1506012..1506242_POSITIVE 377 94 56 56 15 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 361 79 35 45 3 aact:AA00908|5end_probable_sigma-E_factor_regulatory_protein_626076..626306_POSITIVE 360 90 41 205 144 aact:AA02446|5end_conserved_hypothetical_protein_1686841..1687071_POSITIVE 359 99 67 66 22 aact:AA02449|5end_hypothetical_protein_1687575..1687805_POSITIVE 347 56 15 151 114 aact:AA01982|5end_maltodextrin_phosphorylase_1339387..1339617_POSITIVE 346 95 55 153 105 aact:AA01939|5end_NAD(P)_transhydrogenase_subunit_beta_1311039..1311269_POSITIVE 334 99 53 200 140 aact:AA02300|5end_glutamate_racemase_1571348..1571578_POSITIVE 334 80 35 121 63 aact:AA00745|5end_galactokinase_511163..511393_POSITIVE 334 99 53 205 145 aact:AA00623|5end_hypothetical_protein_430457..430687_POSITIVE 334 99 53 197 137 aact:AA00414|5end_possible_histidinol-phosphatase_280899..281129_POSITIVE 324 60 11 211 162 aact:AA02573|5end_ABC_transporter,_ATP-binding_protein_1790409..1790639_POSITIVE 324 90 43 109 62 aact:AA01169|5end_hypothetical_protein_801985..802215_POSITIVE 309 109 69 49 7 aact:AA01408|5end_conserved_hypothetical_protein_950191..950421_POSITIVE 309 96 51 173 128 aact:AA00482|5end_hydrolase_(haloacid_dehalogenase-like)_(HAD_superfamily)_335232..335462_POSITIVE 308 97 51 164 128 aact:AA01458|5end_aminotransferase_978820..979050_POSITIVE 308 105 63 143 102 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 302 60 23 144 105 aact:AA02570|5end_conserved_hypothetical_protein_1789150..1789380_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 395 90 41 85 23 aact:AA01769|3end_hypothetical_protein_1193132..1193282_POSITIVE 368 94 61 41 8 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 361 96 52 44 1 aact:AA02210|3end_ribonucleoside-diphosphate_reductase_alpha_chain_1505956..1506106_POSITIVE 360 90 41 56 3 aact:AA01169|3end_hypothetical_protein_802229..802379_POSITIVE 347 56 15 38 1 aact:AA01981|3end_4-alpha-glucanotransferase_1339354..1339504_POSITIVE 346 95 55 139 91 aact:AA01938|3end_NAD(P)_transhydrogenase_subunit_alpha_1311105..1311255_POSITIVE 334 80 35 124 66 aact:AA00744|3end_aldose_1-epimerase_(mutarotase)_511246..511396_POSITIVE 324 90 43 62 15 aact:AA01168|3end_6-phosphogluconolactonase_802018..802168_POSITIVE 321 79 34 79 25 aact:AA01001|3end_conserved_hypothetical_protein_699974..700124_POSITIVE 321 53 11 123 74 aact:AA00026|3end_conserved_hypothetical_protein_20542..20692_POSITIVE 313 120 71 76 16 aact:AA02954|3end_penicillin_tolerance_protein;_hydroxymethylbutenyl_pyrophosphate_reductase_2066631..2066781_POSITIVE 309 96 51 60 15 aact:AA00480|3end_mannose-specific_phosphotransferase_system_IID_335199..335349_POSITIVE 308 97 51 72 36 aact:AA01457|3end_D-xylose_ABC_transporter,_membrane_spanning_protein_978808..978958_POSITIVE 308 105 63 60 19 aact:AA01172|3end_conserved_hypothetical_protein_803847..803997_POSITIVE 303 79 32 73 28 aact:AA02444|3end_hypothetical_protein_1686635..1686785_POSITIVE 302 60 23 98 59 aact:AA02569|3end_hypothetical_protein_1789184..1789334_POSITIVE 299 66 25 78 34 aact:AA01054|3end_conserved_hypothetical_protein_733940..734090_POSITIVE 298 116 72 85 47 aact:AA00530|3end_hypothetical_protein_362668..362818_POSITIVE 287 80 36 146 97 aact:AA02208|3end_tryptophan_synthase,__alpha_subunit_1503996..1504146_POSITIVE 284 46 4 133 96 aact:AA01601|3end_PTS_system,_glucose-specific_IIA_componen_1082846..1082996_POSITIVE