Origin IGS: taataaaccatttcttgccttcccctgcctgcgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaaggaggcaagatccccctcagcctacagcaacttccccgtaaacgggtggatccaagggaaacgaatttcaataaaaacacattaaaaaacgcatgccaccattttaggaagagacaac .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gttgtctcttcctaaaatggtggcatgcgttttttaatgtgtttttattgaaattcgtttcccttggatccacccgtttacggggaagttgctgtaggctgagggggatcttgcctccttccgcccttcgggcacgttattcgcaagctcggaaactcacttactcacccctttggggtcgttggcagagccaacgctcaaaacgcattgcgttttgtcccccgcaggcaggggaaggcaagaaatggtttatta Mask Tandem Repeat Region ================================================ taataaaccatttcttgccttcccctgcctgcgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaaggaggcaagatccccctcagcctacagcaacttccccgtaaacgggtggatccaagggaaacgaatttcaataaaaacacattaaaaaacgcatgccaccattttaggaagagacaac Find is-nt database================================================ Query_seq: AA01167:AA01168|AA01167:AA01168:glucose-6-phosphate 1-dehydrogenase:6-phosphogluconolactonase:->->:801128..801382 255 taataaaccatttcttgccttcccctgcctgcgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaaggaggcaagatccccctcagcctacagcaacttccccgtaaacgggtggatccaagggaaacgaatttcaataaaaacacattaaaaaacgcatgccaccattttaggaagagacaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01167:AA01168|AA01167:AA01168:glucose-6-phosphate 1-dehydrogenase:6-phosphogluconolactonase:->->:801128..801382 255 taataaaccatttcttgccttcccctgcctgcgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaaggaggcaagatccccctcagcctacagcaacttccccgtaaacgggtggatccaagggaaacgaatttcaataaaaacacattaaaaaacgcatgccaccattttaggaagagacaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01167:AA01168|AA01167:AA01168:glucose-6-phosphate 1-dehydrogenase:6-phosphogluconolactonase:->->:801128..801382 255 taataaaccatttcttgccttcccctgcctgcgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaaggaggcaagatccccctcagcctacagcaacttccccgtaaacgggtggatccaagggaaacgaatttcaataaaaacacattaaaaaacgcatgccaccattttaggaagagacaac Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taataaaccatttcttgccttcccctgcctgcgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaaggaggcaagatccccctcagcctacagcaacttccccgtaaacgggtggatccaagggaaacgaatttcaataaaaacacattaaaaaacgcatgccaccattttaggaagagacaac Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 3 End: 92 .. M N H F L P S P A C G G Q N A M R F E R W L C Q R P Q R G E ................................................................................................................................................................... Protein_Len: 33 Strand: + Start: 121 End: 219 ........................................................................................................................ M P E G R K E A R S P S A Y S N F P V N G W I Q G K R I S I K T H .................................... Protein_Len: 36 Strand: + Start: 147 End: 254 .................................................................................................................................................. M P L S L Q Q L P R K R V D P R E T N F N K N T L K N A C H H F R K R Q . Protein_Len: 60 Strand: - Start: 2 End: 217 . C F A I R K S R Q S Q W R G W L P S Y T L K R A Q S Y R A R L A S P P L I G R L R C C S G R L R T S G L S V F K L L F M ...................................... Protein_Len: 39 Strand: - Start: 1 End: 117 L L G N R A K G Q R R P V F R L A N Q A N A R G V V G F P H T L S N G L K R M .......................................................................................................................................... taataaaccatttcttgccttcccctgcctgcgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaaggaggcaagatccccctcagcctacagcaacttccccgtaaacgggtggatccaagggaaacgaatttcaataaaaacacattaaaaaacgcatgccaccattttaggaagagacaac Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taataaaccatttcttgccttcccctgcctgcgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaaggaggcaagatccccctcagcctacagcaacttccccgtaaacgggtggatccaagggaaacgaatttcaataaaaacacattaaaaaacgcatgccaccattttaggaagagacaac Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01167:AA01168|AA01167:AA01168:glucose-6-phosphate 1-dehydrogenase:6-phosphogluconolactonase:->->:801128..801382 255 taataaaccatttcttgccttcccctgcctgcgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaaggaggcaagatccccctcagcctacagcaacttccccgtaaacgggtggatccaagggaaacgaatttcaataaaaacacattaaaaaacgcatgccaccattttaggaagagacaac Intra-Species Hit: Count: 4 Min: 1 Max: 255 Len: 255 Subject: aact_AA01167_AA01168|glucose-6-phosphate 1-dehydrogenase:6- phosphogluconolactonase|POSITIVE:POSITIVE|[801128, 801382]|255 HSP 1 e-value: 1.0E-142 bit: 505.0 Len: 255 Query Start:1 Query End:255 Subject Strand: POSITIVE Subject Start: 1 Subject End: 255 taataaaccatttcttgccttcccctgcctgcgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaaggaggcaagatccccctcagcctacagcaacttccccgtaaacgggtggatccaagggaaacgaatttcaataaaaacacattaaaaaacgcatgccaccattttaggaagagacaac taataaaccatttcttgccttcccctgcctgcgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaaggaggcaagatccccctcagcctacagcaacttccccgtaaacgggtggatccaagggaaacgaatttcaataaaaacacattaaaaaacgcatgccaccattttaggaagagacaac Subject: aact_AA01173_AA01174|VapD-homolog:hypothetical protein|POSITIVE:POSITIVE|[804446,804839]|394 HSP 1 e-value: 4.0E-45 bit: 182.0 Len: 108 Query Start:32 Query End:139 Subject Strand: NEGATIVE Subject Start: 190 Subject End: 297 ...............................cgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaagg.................................................................................................................... ...............................cgggggacaaaacgcaatgcgttttgagcgttggttctgccaacgaccccaaaggggtaagcaagtgagtttccgagcttgcgaataacgtacccgaagggcggaagg.................................................................................................................... Subject: aact_AA00776_AA00777|conserved hypothetical protein:soluble lytic murein transglycosylase|POSITIVE:POSITIVE|[533930,534458]|529 HSP 1 e-value: 9.0E-43 bit: 174.0 Len: 108 Query Start:32 Query End:139 Subject Strand: NEGATIVE Subject Start: 213 Subject End: 320 ...............................cgggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaagg.................................................................................................................... ...............................cgggggacaaaacgcattgcgttttgagcgttggctctgccaacgaccccaaaggggtgagcaagtaagttttcgaacttgcgaataacgtgcccgaagggcggaagg.................................................................................................................... Subject: aact_AA00861_AA00862|transcription repair coupling factor:conserved hypothetical protein|POSITIVE:POSITIVE|[596211,596604]|394 HSP 1 e-value: 9.0E-40 bit: 165.0 Len: 107 Query Start:33 Query End:139 Subject Strand: POSITIVE Subject Start: 125 Subject End: 231 ................................gggggacaaaacgcaatgcgttttgagcgttggctctgccaacgaccccaaaggggtgagtaagtgagtttccgagcttgcgaataacgtgcccgaagggcggaagg.................................................................................................................... ................................gggggacaaaacgcattgcgttttgagcgttggctctgccaacgcccccaaaggggtgagcaagtaagtttacgaacttgcgaataacgtgcccgaagggcggaagg.................................................................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAAACCAUUUCUUGCCUUCCCCUGCCUGCGGGGGACAAAACGCAAUGCGUUUUGAGCGUUGGCUCUGCCAACGACCCCAAAGGGGUGAGUAAGUGAGUUUCCGAGCUUGCGAAUAACGUGCCCGAAGGGCGGAAGGAGGCAAGAUCCCCCUCAGCCUACAGCAACUUCCCCGUAAACGGGUGGAUCCAAGGGAAACGAAUUUCAAUAAAAACACAUUAAAAAACGCAUGCCACCAUUUUAGGAAGAGACAAC ............((((((((((..((((((..((((..((((((((...))))))))..(((((((...))))))).))))....((((..((..(..((((....))))..).....))..))))...))))))..)))))))))).....(((..((((((((...(((((((....)))).)))......(....)............................)).)).........))))..)))..... (-83.60) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GUUGUCUCUUCCUAAAAUGGUGGCAUGCGUUUUUUAAUGUGUUUUUAUUGAAAUUCGUUUCCCUUGGAUCCACCCGUUUACGGGGAAGUUGCUGUAGGCUGAGGGGGAUCUUGCCUCCUUCCGCCCUUCGGGCACGUUAUUCGCAAGCUCGGAAACUCACUUACUCACCCCUUUGGGGUCGUUGGCAGAGCCAACGCUCAAAACGCAUUGCGUUUUGUCCCCCGCAGGCAGGGGAAGGCAAGAAAUGGUUUAUUA .((.((((..((((....((..((..(((..((((((((......))))))))..)))(((((.(((((......))))).))))).))..)).))))..)))).)).((((((((.((((.(((.(((((((.((.....))...))))))).....................((((.(((((((...)))))))..((((((((...))))))))..))))...))).)))).))))))))............ (-85.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 801 60 13 217 170 aact:AA01174|5end_hypothetical_protein_804700..804930_POSITIVE 792 96 51 231 186 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 684 99 53 136 89 aact:AA01098|5end_bis(5'-nucleosyl)-tetraphosphatase_757699..757929_POSITIVE 581 99 51 199 148 aact:AA02108|5end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1433393..1433623_POSITIVE 559 99 54 47 1 aact:AA02106|5end_hypothetical_protein_1433241..1433471_POSITIVE 539 97 51 230 182 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 536 90 45 156 111 aact:AA01633|5end_probable_ferredoxin-like_protein_1104008..1104238_POSITIVE 500 99 51 199 148 aact:AA01090|5end_leucyl-tRNA_synthetase,_leucyl-tRNA_ligase_753548..753778_POSITIVE 466 97 52 189 143 aact:AA02666|5end_DNA_helicase_II_1854410..1854640_POSITIVE 444 99 57 43 1 aact:AA01089|5end_hypothetical_protein_753392..753622_POSITIVE 408 85 43 62 11 aact:AA02933|5end_conserved_hypothetical_protein_(HesA/MoeB/ThiF_family_protein)_2053467..2053697_POSITIVE 395 86 41 142 95 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 393 70 24 80 34 aact:AA00652|5end_arginyl-tRNA_synthetase_(arginine--tRNA_ligase)_445718..445948_POSITIVE 390 48 9 76 36 aact:AA00884|5end_sodium-dependent_transporter_614392..614622_POSITIVE 388 156 112 195 123 aact:AA00785|5end_conserved_hypothetical_protein_(possible_integrase)_539221..539451_POSITIVE 387 109 61 110 43 aact:AA02538|5end_conserved_hypothetical_protein_1765187..1765417_POSITIVE 386 149 104 169 114 aact:AA00291|5end_hypothetical_protein_201715..201945_POSITIVE 381 90 45 170 132 aact:AA00991|5end_conserved_hypothetical_protein_693191..693421_POSITIVE 380 43 8 231 189 aact:AA02949|5end_hypothetical_protein_2061944..2062174_POSITIVE 378 51 19 230 200 aact:AA01436|5end_fructose-bisphosphate_aldolase_class_II_962981..963211_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 638 110 61 53 3 aact:AA01097|3end_conserved_hypothetical_protein_757685..757835_POSITIVE 560 88 42 52 6 aact:AA01342|3end_conserved_hypotheticl_protein_(possible_ribosomal_RNA_adenine_dimethylase)_911085..911235_POSITIVE 517 100 51 136 85 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 500 99 51 54 3 aact:AA01088|3end_rare_lipoprotein_B_precursor_753483..753633_POSITIVE 466 97 52 50 4 aact:AA02663|3end_DNA_ligase_1854351..1854501_POSITIVE 408 85 43 59 8 aact:AA02932|3end_lytic_murein_transglycosylase_A_2053544..2053694_POSITIVE 395 86 41 81 34 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 390 48 9 71 31 aact:AA00883|3end_hypothetical_protein_614467..614617_POSITIVE 389 55 12 95 42 aact:AA02947|3end_riboflavin_biosynthesis_protein_2061874..2062024_POSITIVE 387 109 61 106 39 aact:AA02536|3end_conserved_hypothetical_protein_1765263..1765413_POSITIVE 381 67 25 148 102 aact:AA02106|3end_hypothetical_protein_1433455..1433605_POSITIVE 381 175 131 105 61 aact:AA02103|3end_D-alanyl-D-alanine-endopeptidase;_penicillin-binding_protein_7_1431784..1431934_POSITIVE 375 60 13 115 60 aact:AA01890|3end_cell-cycle_protein_1275967..1276117_POSITIVE 363 92 51 82 29 aact:AA01194|3end_acyl_carrier_protein_819923..820073_POSITIVE 362 144 101 88 48 aact:AA01059|3end_orotate_phosphoribosyltransferase_735773..735923_POSITIVE 362 77 31 123 65 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 361 67 22 118 57 aact:AA01822|3end_lysostaphin_precursor_1227671..1227821_POSITIVE 358 80 31 149 100 aact:AA01089|3end_hypothetical_protein_753597..753747_POSITIVE 356 149 104 90 29 aact:AA02994|3end_probable_cation_transport_protein_2093316..2093466_POSITIVE 356 66 26 64 23 aact:AA00686|3end_hypothetical_protein_469219..469369_POSITIVE