Origin IGS: gacgttttccggcagctcggcaaaatgctcacgccagtaaggcagtttttgtttgtctacagcaacgatattgcataaaaattgaccgtactttgcttaaccggattagaattcggcgttaacctta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaggttaacgccgaattctaatccggttaagcaaagtacggtcaatttttatgcaatatcgttgctgtagacaaacaaaaactgccttactggcgtgagcattttgccgagctgccggaaaacgtc Mask Tandem Repeat Region ================================================ gacgttttccggcagctcggcaaaatgctcacgccagtaaggcagtttttgtttgtctacagcaacgatattgcataaaaattgaccgtactttgcttaaccggattagaattcggcgttaacctta Find is-nt database================================================ Query_seq: AA01155:AA01156|AA01155:AA01156:hypothetical protein:chaperonin heat shock protein 33 homolog:->->:791472..791598 127 gacgttttccggcagctcggcaaaatgctcacgccagtaaggcagtttttgtttgtctacagcaacgatattgcataaaaattgaccgtactttgcttaaccggattagaattcggcgttaacctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01155:AA01156|AA01155:AA01156:hypothetical protein:chaperonin heat shock protein 33 homolog:->->:791472..791598 127 gacgttttccggcagctcggcaaaatgctcacgccagtaaggcagtttttgtttgtctacagcaacgatattgcataaaaattgaccgtactttgcttaaccggattagaattcggcgttaacctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01155:AA01156|AA01155:AA01156:hypothetical protein:chaperonin heat shock protein 33 homolog:->->:791472..791598 127 gacgttttccggcagctcggcaaaatgctcacgccagtaaggcagtttttgtttgtctacagcaacgatattgcataaaaattgaccgtactttgcttaaccggattagaattcggcgttaacctta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 gacgttttccggcagctcggcaaaatgctcacgccagtaaggcagtttttgtttgtctacagcaacgatattgcataaaaattgaccgtactttgcttaaccggattagaattcggcgttaacctta Predict ORF larger than 30AA ================================================ gacgttttccggcagctcggcaaaatgctcacgccagtaaggcagtttttgtttgtctacagcaacgatattgcataaaaattgaccgtactttgcttaaccggattagaattcggcgttaacctta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ gacgttttccggcagctcggcaaaatgctcacgccagtaaggcagtttttgtttgtctacagcaacgatattgcataaaaattgaccgtactttgcttaaccggattagaattcggcgttaacctta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01155:AA01156|AA01155:AA01156:hypothetical protein:chaperonin heat shock protein 33 homolog:->->:791472..791598 127 gacgttttccggcagctcggcaaaatgctcacgccagtaaggcagtttttgtttgtctacagcaacgatattgcataaaaattgaccgtactttgcttaaccggattagaattcggcgttaacctta Intra-Species Hit: Count: 1 Min: 1 Max: 127 Len: 127 Subject: aact_AA01155_AA01156|hypothetical protein:chaperonin heat shock protein 33 homolog|POSITIVE:POSITIVE|[791472,791598]|127 HSP 1 e-value: 2.0E-66 bit: 252.0 Len: 127 Query Start:1 Query End:127 Subject Strand: POSITIVE Subject Start: 1 Subject End: 127 gacgttttccggcagctcggcaaaatgctcacgccagtaaggcagtttttgtttgtctacagcaacgatattgcataaaaattgaccgtactttgcttaaccggattagaattcggcgttaacctta gacgttttccggcagctcggcaaaatgctcacgccagtaaggcagtttttgtttgtctacagcaacgatattgcataaaaattgaccgtactttgcttaaccggattagaattcggcgttaacctta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GACGUUUUCCGGCAGCUCGGCAAAAUGCUCACGCCAGUAAGGCAGUUUUUGUUUGUCUACAGCAACGAUAUUGCAUAAAAAUUGACCGUACUUUGCUUAACCGGAUUAGAAUUCGGCGUUAACCUUA ((((((.(((((.(((..(((...........))).(((.((((((((((((.((((.........))))....)))))))))).)).)))...)))...))))).........))))))....... (-29.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGGUUAACGCCGAAUUCUAAUCCGGUUAAGCAAAGUACGGUCAAUUUUUAUGCAAUAUCGUUGCUGUAGACAAACAAAAACUGCCUUACUGGCGUGAGCAUUUUGCCGAGCUGCCGGAAAACGUC ...(((....))).........((((((.......(((.(((.(((.....((((......(((..(((......)))..))).(((.....)))....)))).)))))).)))))))))....... (-30.01) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 57 11 89 31 aact:AA01830|5end_2,3-cyclic-nucleotide_2-phosphodiesterase_1231556..1231786_POSITIVE 368 48 9 99 64 aact:AA01923|5end_type_III_restriction-modification_system_methylation_subunit_1297487..1297717_POSITIVE 367 56 12 64 26 aact:AA02399|5end_30S_ribosomal_protein_S21_1650900..1651130_POSITIVE 361 55 11 62 17 aact:AA02477|5end_PTS_system,_N-acetylglucosamine-permease_IIABC_component_1712989..1713219_POSITIVE 349 116 75 207 162 aact:AA01251|5end_hypothetical_protein_851825..852055_POSITIVE 345 45 3 193 161 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 343 120 83 163 127 aact:AA01519|5end_conserved_hypothetical_protein_1021794..1022024_POSITIVE 342 44 9 51 24 aact:AA01875|5end_PTS_system,_glucose-specific_enzyme_II,_A_component_1262563..1262793_POSITIVE 338 48 2 120 65 aact:AA01527|5end_hypothetical_protein_1025704..1025934_POSITIVE 337 58 11 190 146 aact:AA02407|5end_thymidine_kinase_1652666..1652896_POSITIVE 329 120 77 172 128 aact:AA01008|5end_mazG_beta-lactamase_regulatory_protein_homolog_703470..703700_POSITIVE 324 47 3 77 32 aact:AA02964|5end_opacity_associated_protein_A_2072738..2072968_POSITIVE 317 116 71 231 194 aact:AA00602|5end_1-deoxy-D-xylulose_5-phosphate_reductoisomerase_409077..409307_POSITIVE 316 46 1 118 69 aact:AA02758|5end_3-phosphoshikimate_1-carboxyvinyltransferase_1931802..1932032_POSITIVE 312 118 71 102 48 aact:AA01745|5end_conserved_hypothetical_protein_(possible_peptide_chain_release_factor_2)_1180394..1180624_POSITIVE 312 120 83 162 121 aact:AA01554|5end_conserved_hypothetical_protein;_hypothetical_membrane_protein_1042061..1042291_POSITIVE 312 42 8 67 21 aact:AA01423|5end_cell_division_protein_X,_ABC_system_permease_956668..956898_POSITIVE 311 43 1 66 32 aact:AA00578|5end_DNA_topoisomerase_394576..394806_POSITIVE 310 58 14 212 172 aact:AA00870|5end_rough_colony_protein_C_601735..601965_POSITIVE 310 55 11 125 75 aact:AA00761|5end_conserved_hypothetical_protein_(possible_peptidase)_523554..523784_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 381 57 11 115 57 aact:AA01829|3end_hypothetical_protein_1231662..1231812_POSITIVE 349 116 75 82 37 aact:AA01250|3end_nadAB_transcriptional_regulator_851780..851930_POSITIVE 343 120 83 80 44 aact:AA01517|3end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1021791..1021941_POSITIVE 329 120 77 68 24 aact:AA01006|3end_conserved_hypothetical_protein_(possible_cytosine_deaminas)_703446..703596_POSITIVE 329 46 1 85 37 aact:AA00521|3end_phosphatase_358324..358474_POSITIVE 312 120 83 53 12 aact:AA01553|3end_conserved_hypothetical_protein_1042032..1042182_POSITIVE 312 42 8 55 9 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 311 43 1 58 24 aact:AA00576|3end_conserved_hypothetical_protein_(possible_translation_factor)_394648..394798_POSITIVE 306 47 8 49 7 aact:AA00606|3end_membrane-associated_zinc_metalloprotease_413219..413369_POSITIVE 305 68 24 55 15 aact:AA02046|3end_PTS_system_galactitol-specific_enzyme_IIC_1393687..1393837_POSITIVE 304 45 4 69 22 aact:AA01502|3end_serine_acetyltransferase_1010888..1011038_POSITIVE 304 119 74 92 51 aact:AA01158|3end_ribosome_associated_heat_shock_protein_15_homolog_792892..793042_POSITIVE 302 110 63 86 35 aact:AA01370|3end_rod_shape-determining_protein_926586..926736_POSITIVE 302 43 3 63 11 aact:AA00474|3end_cell_division_protein_Y_(GTP-binding_signal_recognition_particle)_331724..331874_POSITIVE 299 94 52 115 71 aact:AA01492|3end_glucose_inhibited_division_protein_B_1003566..1003716_POSITIVE 299 119 72 56 9 aact:AA00592|3end_conserved_hypothetical_protein_(membrane/transport_protein)_407286..407436_POSITIVE 297 47 4 86 50 aact:AA02350|3end_possible_lipoprotein_1609179..1609329_POSITIVE 294 36 3 63 22 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE 293 120 71 147 99 aact:AA02184|3end_anthranilate_isomerase;_indole-3-glycerol_phosphate_synthase;_phosphoribosylanthranilate_isomerase_1492541..1492691_POSITIVE 293 49 3 89 26 aact:AA00525|3end_hypothetical_protein_359459..359609_POSITIVE