Origin IGS: aaaaacctcgttattctgtctaaaattgataagtaaaaacgtgcctgactatacccgttttgcacggtttgaacaatagccagggttgattatttttacatttcttgatttagttcatatcacccttacgccatagggggcaagggctaacgtaacaataaaccgagcagcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagctgctcggtttattgttacgttagcccttgccccctatggcgtaagggtgatatgaactaaatcaagaaatgtaaaaataatcaaccctggctattgttcaaaccgtgcaaaacgggtatagtcaggcacgtttttacttatcaattttagacagaataacgaggttttt Mask Tandem Repeat Region ================================================ aaaaacctcgttattctgtctaaaattgataagtaaaaacgtgcctgactatacccgttttgcacggtttgaacaatagccagggttgattatttttacatttcttgatttagttcatatcacccttacgccatagggggcaagggctaacgtaacaataaaccgagcagcta Find is-nt database================================================ Query_seq: AA01154:AA01155|AA01154:AA01155:sec-independent protein translocase protein:hypothetical protein:->->:791194..791366 173 aaaaacctcgttattctgtctaaaattgataagtaaaaacgtgcctgactatacccgttttgcacggtttgaacaatagccagggttgattatttttacatttcttgatttagttcatatcacccttacgccatagggggcaagggctaacgtaacaataaaccgagcagcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01154:AA01155|AA01154:AA01155:sec-independent protein translocase protein:hypothetical protein:->->:791194..791366 173 aaaaacctcgttattctgtctaaaattgataagtaaaaacgtgcctgactatacccgttttgcacggtttgaacaatagccagggttgattatttttacatttcttgatttagttcatatcacccttacgccatagggggcaagggctaacgtaacaataaaccgagcagcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01154:AA01155|AA01154:AA01155:sec-independent protein translocase protein:hypothetical protein:->->:791194..791366 173 aaaaacctcgttattctgtctaaaattgataagtaaaaacgtgcctgactatacccgttttgcacggtttgaacaatagccagggttgattatttttacatttcttgatttagttcatatcacccttacgccatagggggcaagggctaacgtaacaataaaccgagcagcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaaaacctcgttattctgtctaaaattgataagtaaaaacgtgcctgactatacccgttttgcacggtttgaacaatagccagggttgattatttttacatttcttgatttagttcatatcacccttacgccatagggggcaagggctaacgtaacaataaaccgagcagcta Predict ORF larger than 30AA ================================================ aaaaacctcgttattctgtctaaaattgataagtaaaaacgtgcctgactatacccgttttgcacggtttgaacaatagccagggttgattatttttacatttcttgatttagttcatatcacccttacgccatagggggcaagggctaacgtaacaataaaccgagcagcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaaaacctcgttattctgtctaaaattgataagtaaaaacgtgcctgactatacccgttttgcacggtttgaacaatagccagggttgattatttttacatttcttgatttagttcatatcacccttacgccatagggggcaagggctaacgtaacaataaaccgagcagcta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01154:AA01155|AA01154:AA01155:sec-independent protein translocase protein:hypothetical protein:->->:791194..791366 173 aaaaacctcgttattctgtctaaaattgataagtaaaaacgtgcctgactatacccgttttgcacggtttgaacaatagccagggttgattatttttacatttcttgatttagttcatatcacccttacgccatagggggcaagggctaacgtaacaataaaccgagcagcta Intra-Species Hit: Count: 1 Min: 1 Max: 173 Len: 173 Subject: aact_AA01154_AA01155|sec-independent protein translocase protein:hypothetical protein|POSITIVE:POSITIVE|[791194,791366]|173 HSP 1 e-value: 1.0E-93 bit: 343.0 Len: 173 Query Start:1 Query End:173 Subject Strand: POSITIVE Subject Start: 1 Subject End: 173 aaaaacctcgttattctgtctaaaattgataagtaaaaacgtgcctgactatacccgttttgcacggtttgaacaatagccagggttgattatttttacatttcttgatttagttcatatcacccttacgccatagggggcaagggctaacgtaacaataaaccgagcagcta aaaaacctcgttattctgtctaaaattgataagtaaaaacgtgcctgactatacccgttttgcacggtttgaacaatagccagggttgattatttttacatttcttgatttagttcatatcacccttacgccatagggggcaagggctaacgtaacaataaaccgagcagcta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAACCUCGUUAUUCUGUCUAAAAUUGAUAAGUAAAAACGUGCCUGACUAUACCCGUUUUGCACGGUUUGAACAAUAGCCAGGGUUGAUUAUUUUUACAUUUCUUGAUUUAGUUCAUAUCACCCUUACGCCAUAGGGGGCAAGGGCUAACGUAACAAUAAACCGAGCAGCUA ......((((......((((.......)))).((..((((((((..(((.......)))..)))).))))..))..(((((.((((.(((.....(((.(((....))).))).....)))))))....(((......)))...)))))..............))))...... (-34.70) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCUGCUCGGUUUAUUGUUACGUUAGCCCUUGCCCCCUAUGGCGUAAGGGUGAUAUGAACUAAAUCAAGAAAUGUAAAAAUAAUCAACCCUGGCUAUUGUUCAAACCGUGCAAAACGGGUAUAGUCAGGCACGUUUUUACUUAUCAAUUUUAGACAGAAUAACGAGGUUUUU ......((((.....(((((.(((..((((((((((.....)).))))))))...)))...........((...((((((((.......(((((((((........((((.....))))..)))))))))...))))))))...)).......))))).....))))...... (-44.90) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 358 83 41 156 120 aact:AA02402|5end_O-sialoglycoprotein_endopeptidase_1651337..1651567_POSITIVE 346 150 114 218 189 aact:AA01735|5end_hypothetical_protein_1172533..1172763_POSITIVE 338 165 121 211 166 aact:AA02220|5end_ABC_transport_system_permease_protein_1511155..1511385_POSITIVE 334 170 122 182 118 aact:AA01609|5end_p-aminobenzoate_synthase_component_I_homolog_1087903..1088133_POSITIVE 328 152 113 179 143 aact:AA01355|5end_large_subunit_pseudouridylate_synthase_D_917228..917458_POSITIVE 327 90 43 80 30 aact:AA00309|5end_3-hydroxyisobutyrate_dehydrogenase;_2-hydroxy-3-oxopropionate_reductase_209285..209515_POSITIVE 322 90 44 72 27 aact:AA02363|5end_hflK_protein_1620744..1620974_POSITIVE 321 88 41 187 141 aact:AA02456|5end_conserved_hypothetical_protein_1694743..1694973_POSITIVE 319 153 120 54 21 aact:AA01953|5end_hypothetical_protein_1320132..1320362_POSITIVE 318 165 121 215 158 aact:AA00974|5end_glucosamine-fructose-6-phosphate_679215..679445_POSITIVE 316 146 102 69 26 aact:AA02421|5end_conserved_hypothetical_protein_1667044..1667274_POSITIVE 316 146 102 75 32 aact:AA02297|5end_conserved_hypothetical_protein_1569620..1569850_POSITIVE 316 146 102 75 32 aact:AA00621|5end_conserved_hypothetical_protein_428727..428957_POSITIVE 316 146 102 75 32 aact:AA00412|5end_conserved_hypothetical_protein_279174..279404_POSITIVE 310 88 44 226 186 aact:AA00837|5end_cell_division_protein_W_576317..576547_POSITIVE 310 153 113 175 133 aact:AA00054|5end_cytochrome_c_biogenesis_protein_41393..41623_POSITIVE 309 90 41 197 146 aact:AA00292|5end_dihydrofolate_reductase_201800..202030_POSITIVE 309 90 41 112 61 aact:AA00291|5end_hypothetical_protein_201715..201945_POSITIVE 303 168 129 179 127 aact:AA02478|5end_PTS_system,_N-acetylglucosamine-specific_enzyme_IIABC_1713319..1713549_POSITIVE 299 168 130 64 20 aact:AA01274|5end_hypothetical_protein_864519..864749_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 358 83 41 130 94 aact:AA02400|3end_hypothetical_protein_1651391..1651541_POSITIVE 327 163 122 151 112 aact:AA01608|3end_conserved_hypothetical_protein_1087977..1088127_POSITIVE 309 90 41 130 79 aact:AA00290|3end_conserved_hypothetical_protein_201813..201963_POSITIVE 304 88 41 117 49 aact:AA01973|3end_acetate_kinase_1335733..1335883_POSITIVE 303 168 129 151 99 aact:AA02477|3end_PTS_system,_N-acetylglucosamine-permease_IIABC_component_1713371..1713521_POSITIVE 299 168 130 53 9 aact:AA01273|3end_hypothetical_protein_864588..864738_POSITIVE 297 160 112 149 91 aact:AA00973|3end_transcriptional_regulator,_DeoR_family_679237..679387_POSITIVE 293 168 125 94 47 aact:AA01934|3end_conserved_hypothetical_protein_1306993..1307143_POSITIVE 289 169 121 42 1 aact:AA01167|3end_glucose-6-phosphate_1-dehydrogenase_801067..801217_POSITIVE 286 89 43 76 23 aact:AA02324|3end_ABC_transporter_ATP_binding_protein_1587392..1587542_POSITIVE 280 153 120 55 23 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 276 89 41 146 104 aact:AA00424|3end_hypothetical_protein_287403..287553_POSITIVE 274 162 129 45 4 aact:AA03015|3end_hypothetical_protein_2105251..2105401_POSITIVE 274 168 121 84 35 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 273 89 43 109 49 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 272 170 136 44 8 aact:AA01202|3end_integrase/recombinase_824207..824357_POSITIVE 272 80 40 113 69 aact:AA00475|3end_conserved_hypothetical_protein_(possible_N6-adenine-specific_methylase)_332282..332432_POSITIVE 271 97 56 95 53 aact:AA00933|3end_ribose_ABC_transporter,_ATP-binding_protein_645197..645347_POSITIVE 270 169 121 63 2 aact:AA01407|3end_outer_membrane_antigenic_lipoprotein_b_precursor_950251..950401_POSITIVE 268 153 123 89 47 aact:AA01694|3end_PTS_system,_IIA_component_1150467..1150617_POSITIVE