Origin IGS: taaagccaaaaatgtgacaatgcaagacggttgctgagcttgtcgaagcatgaatcttgcgtcgttcttagttaaaatcacttatggttcgacaagttcaccgatcgtgatttcaggttgagggactaaaaaacgccgtcaattctgaccgcacttttcacataaataaaaagccgaataacatattcggcttttcgtttttatgttatcggaacta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagttccgataacataaaaacgaaaagccgaatatgttattcggctttttatttatgtgaaaagtgcggtcagaattgacggcgttttttagtccctcaacctgaaatcacgatcggtgaacttgtcgaaccataagtgattttaactaagaacgacgcaagattcatgcttcgacaagctcagcaaccgtcttgcattgtcacatttttggcttta Mask Tandem Repeat Region ================================================ taaagccaaaaatgtgacaatgcaagacggttgctgagcttgtcgaagcatgaatcttgcgtcgttcttagttaaaatcacttatggttcgacaagttcaccgatcgtgatttcaggttgagggactaaaaaacgccgtcaattctgaccgcacttttcacataaataaaaagccgaataacatattcggcttttcgtttttatgttatcggaacta Find is-nt database================================================ Query_seq: AA01149:AA01151|AA01149:AA01151:glycyl-tRNA synthetase beta subunit:porphobilinogen synthase (delta-aminolevulinic acid dehydratase):->->:788246..788462 217 taaagccaaaaatgtgacaatgcaagacggttgctgagcttgtcgaagcatgaatcttgcgtcgttcttagttaaaatcacttatggttcgacaagttcaccgatcgtgatttcaggttgagggactaaaaaacgccgtcaattctgaccgcacttttcacataaataaaaagccgaataacatattcggcttttcgtttttatgttatcggaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01149:AA01151|AA01149:AA01151:glycyl-tRNA synthetase beta subunit:porphobilinogen synthase (delta-aminolevulinic acid dehydratase):->->:788246..788462 217 taaagccaaaaatgtgacaatgcaagacggttgctgagcttgtcgaagcatgaatcttgcgtcgttcttagttaaaatcacttatggttcgacaagttcaccgatcgtgatttcaggttgagggactaaaaaacgccgtcaattctgaccgcacttttcacataaataaaaagccgaataacatattcggcttttcgtttttatgttatcggaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01149:AA01151|AA01149:AA01151:glycyl-tRNA synthetase beta subunit:porphobilinogen synthase (delta-aminolevulinic acid dehydratase):->->:788246..788462 217 taaagccaaaaatgtgacaatgcaagacggttgctgagcttgtcgaagcatgaatcttgcgtcgttcttagttaaaatcacttatggttcgacaagttcaccgatcgtgatttcaggttgagggactaaaaaacgccgtcaattctgaccgcacttttcacataaataaaaagccgaataacatattcggcttttcgtttttatgttatcggaacta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaagccaaaaatgtgacaatgcaagacggttgctgagcttgtcgaagcatgaatcttgcgtcgttcttagttaaaatcacttatggttcgacaagttcaccgatcgtgatttcaggttgagggactaaaaaacgccgtcaattctgaccgcacttttcacataaataaaaagccgaataacatattcggcttttcgtttttatgttatcggaacta Predict ORF larger than 30AA ================================================ taaagccaaaaatgtgacaatgcaagacggttgctgagcttgtcgaagcatgaatcttgcgtcgttcttagttaaaatcacttatggttcgacaagttcaccgatcgtgatttcaggttgagggactaaaaaacgccgtcaattctgaccgcacttttcacataaataaaaagccgaataacatattcggcttttcgtttttatgttatcggaacta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaagccaaaaatgtgacaatgcaagacggttgctgagcttgtcgaagcatgaatcttgcgtcgttcttagttaaaatcacttatggttcgacaagttcaccgatcgtgatttcaggttgagggactaaaaaacgccgtcaattctgaccgcacttttcacataaataaaaagccgaataacatattcggcttttcgtttttatgttatcggaacta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 43 HP_score: -3.4 Tail_Score: -2.66009 Start: 22 End: 34 Full_Region: gcttcgacaagctca gcaa ccgtc ttgc attgtcacatttttg .....................gcaaccgtcttgc....................................................................................................................................................................................... TransTerm Strand: - Conf: 74 HP_score: -4.2 Tail_Score: -5.1675 Start: 134 End: 153 Full_Region: tatttatgtgaaaag tgcggtca gaat tgacggcg ttttttagtccctca .....................................................................................................................................tgcggtcagaattgacggcg................................................................ TransTerm Strand: + Conf: 93 HP_score: -10.6 Tail_Score: -5.1523 Start: 167 End: 197 Full_Region: CACTTTTCACATAAA TAAAAAGCCGAATA ACA TATTCGGCTTTTCG TTTTTATGTTATCGG ......................................................................................................................................................................TAAAAAGCCGAATAACATATTCGGCTTTTCG.................... TransTerm Strand: + Conf: 89 HP_score: -11.9 Tail_Score: -3.68053 Start: 168 End: 196 Full_Region: ACTTTTCACATAAAT AAAAAGCCGAATA ACA TATTCGGCTTTTC GTTTTTATGTTATCG .......................................................................................................................................................................AAAAAGCCGAATAACATATTCGGCTTTTC..................... TransTerm Strand: + Conf: 100 HP_score: -11.8 Tail_Score: -4.84033 Start: 173 End: 191 Full_Region: TCACATAAATAAAAA GCCGAATA ACA TATTCGGC TTTTCGTTTTTATGT ............................................................................................................................................................................GCCGAATAACATATTCGGC.......................... Find igs database================================================ Query_seq: AA01149:AA01151|AA01149:AA01151:glycyl-tRNA synthetase beta subunit:porphobilinogen synthase (delta-aminolevulinic acid dehydratase):->->:788246..788462 217 taaagccaaaaatgtgacaatgcaagacggttgctgagcttgtcgaagcatgaatcttgcgtcgttcttagttaaaatcacttatggttcgacaagttcaccgatcgtgatttcaggttgagggactaaaaaacgccgtcaattctgaccgcacttttcacataaataaaaagccgaataacatattcggcttttcgtttttatgttatcggaacta Intra-Species Hit: Count: 1 Min: 1 Max: 217 Len: 217 Subject: aact_AA01149_AA01151|glycyl-tRNA synthetase beta subunit:porphobilinogen synthase (delta-aminolevulinic acid dehydratase)|POSITIVE:POSITIVE|[788246,788462]|217 HSP 1 e-value: 1.0E-120 bit: 430.0 Len: 217 Query Start:1 Query End:217 Subject Strand: POSITIVE Subject Start: 1 Subject End: 217 taaagccaaaaatgtgacaatgcaagacggttgctgagcttgtcgaagcatgaatcttgcgtcgttcttagttaaaatcacttatggttcgacaagttcaccgatcgtgatttcaggttgagggactaaaaaacgccgtcaattctgaccgcacttttcacataaataaaaagccgaataacatattcggcttttcgtttttatgttatcggaacta taaagccaaaaatgtgacaatgcaagacggttgctgagcttgtcgaagcatgaatcttgcgtcgttcttagttaaaatcacttatggttcgacaagttcaccgatcgtgatttcaggttgagggactaaaaaacgccgtcaattctgaccgcacttttcacataaataaaaagccgaataacatattcggcttttcgtttttatgttatcggaacta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAGCCAAAAAUGUGACAAUGCAAGACGGUUGCUGAGCUUGUCGAAGCAUGAAUCUUGCGUCGUUCUUAGUUAAAAUCACUUAUGGUUCGACAAGUUCACCGAUCGUGAUUUCAGGUUGAGGGACUAAAAAACGCCGUCAAUUCUGACCGCACUUUUCACAUAAAUAAAAAGCCGAAUAACAUAUUCGGCUUUUCGUUUUUAUGUUAUCGGAACUA .....((....((((((.(((((...(((((((.(((((((((((((.(((((............................))))).))))))))))))).)))))))....(((((((((.((.(........))).)))).)))))..))).)).))))))(((..((((((((((((...))))))))))))..)))..........))..... (-69.09) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGUUCCGAUAACAUAAAAACGAAAAGCCGAAUAUGUUAUUCGGCUUUUUAUUUAUGUGAAAAGUGCGGUCAGAAUUGACGGCGUUUUUUAGUCCCUCAACCUGAAAUCACGAUCGGUGAACUUGUCGAACCAUAAGUGAUUUUAACUAAGAACGACGCAAGAUUCAUGCUUCGACAAGCUCAGCAACCGUCUUGCAUUGUCACAUUUUUGGCUUUA ..((.......(((((((...(((((((((((((...))))))))))))).))))))).......)).(((((((.(((((.(((((((.(((.........(((((((((....(((...........)))....)))))))))))))))))))..(((((((...((((..((.....))))))...)))))))..)))))...))))))).... (-60.34) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 584 116 80 227 191 aact:AA02569|5end_hypothetical_protein_1789012..1789242_POSITIVE 442 48 4 205 158 aact:AA01954|5end_excinuclease_ABC,_subunit_C_1320468..1320698_POSITIVE 408 157 119 127 92 aact:AA01202|5end_integrase/recombinase_823240..823470_POSITIVE 391 158 119 143 92 aact:AA01416|5end_2C-methyl-D-erythritol_2,4-cyclodiphosphate_952780..953010_POSITIVE 386 159 115 186 137 aact:AA00679|5end_cytoplasmic_axial_filament_(Ribonuclease_G)_463288..463518_POSITIVE 381 64 22 58 15 aact:AA01900|5end_hypothetical_protein_1282233..1282463_POSITIVE 379 159 126 40 7 aact:AA01660|5end_hypothetical_protein_1125035..1125265_POSITIVE 378 47 14 36 6 aact:AA02390|5end_mannonate_dehydratase_1639303..1639533_POSITIVE 372 158 116 177 127 aact:AA01564|5end_transaldolase_B_1049099..1049329_POSITIVE 371 180 131 200 142 aact:AA02706|5end_2-oxoglutarate_dehydrogenase_E1_component_(decarboxylase_component)_1886843..1887073_POSITIVE 371 180 131 74 16 aact:AA02705|5end_hypothetical_protein_1886717..1886947_POSITIVE 367 157 112 126 78 aact:AA02980|5end_conserved_hypothetical_protein_2083305..2083535_POSITIVE 360 157 122 122 86 aact:AA01315|5end_DNA/pantothenate_metabolism_flavoprotein_890091..890321_POSITIVE 360 157 122 52 16 aact:AA01314|5end_hypothetical_protein_890021..890251_POSITIVE 354 180 141 220 177 aact:AA00460|5end_ATP-dependent_ClpY-related_protease;_heat_shock_protein_321023..321253_POSITIVE 354 180 141 48 5 aact:AA00459|5end_hypothetical_protein_320851..321081_POSITIVE 351 159 115 124 79 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 351 157 115 121 75 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 351 66 22 112 53 aact:AA00835|5end_UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapep_tide)_575212..575442_POSITIVE 349 154 118 231 191 aact:AA00533|5end_conserved_hypothetical_protein_365323..365553_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 408 157 119 110 75 aact:AA01201|3end_phosphatase;_hydrolase_823303..823453_POSITIVE 391 158 119 143 92 aact:AA01413|3end_conserved_hypothetical_protein_952860..953010_POSITIVE 386 159 115 66 17 aact:AA00675|3end_glutaredoxin_2_463248..463398_POSITIVE 371 180 131 72 14 aact:AA02704|3end_dihydrolipoamide_succinyltransferase_1886795..1886945_POSITIVE 367 157 112 49 1 aact:AA02978|3end_sufI_protein_precursor_homolog_2083308..2083458_POSITIVE 360 157 122 147 111 aact:AA01314|3end_hypothetical_protein_890196..890346_POSITIVE 360 157 122 50 14 aact:AA01313|3end_deoxyuridinetriphosphatase;_deoxyuridine_5'-triphosphate_nucleotidohydrolase_890099..890249_POSITIVE 357 158 129 41 12 aact:AA01563|3end_transketolase_isozyme_1_1049043..1049193_POSITIVE 351 157 115 60 14 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 347 157 111 106 64 aact:AA01021|3end_hypothetical_protein_714276..714426_POSITIVE 347 158 116 132 85 aact:AA00158|3end_ampD_signalling_protein_(N-acetylmuramyl-L-alanine_amidase)_112935..113085_POSITIVE 334 158 111 69 5 aact:AA02959|3end_pyrroline-5-carboxylate_(P5C)_reductase_2070976..2071126_POSITIVE 329 150 101 59 2 aact:AA02740|3end_conserved_hypothetical_protein_1921394..1921544_POSITIVE 328 64 21 55 10 aact:AA01987|3end_hypothetical_protein_1350620..1350770_POSITIVE 327 158 116 91 55 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 326 115 75 85 45 aact:AA01953|3end_hypothetical_protein_1320349..1320499_POSITIVE 320 158 116 74 35 aact:AA00470|3end_conserved_hypothetical_protein_(possible_Zn-dependent_protease_with_chaperone_function)_327659..327809_POSITIVE 319 158 114 150 114 aact:AA01520|3end_hypothetical_protein_1022887..1023037_POSITIVE 318 49 5 71 27 aact:AA01651|3end_hypothetical_protein_1120004..1120154_POSITIVE 312 162 130 57 26 aact:AA00345|3end_transcription_antitermination_protein_232058..232208_POSITIVE