Origin IGS: taaaaaatacctggaaaactgaccgcactttaaaggaaagttatgtcgctgatgaccacagaaaatacctttacaacagaaatcatcgagcttgttcacaatacgaaacagcgcatggctgtagcggtgaaaaagaagagagaatgatttttaaaataagaagagaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttctcttcttattttaaaaatcattctctcttctttttcaccgctacagccatgcgctgtttcgtattgtgaacaagctcgatgatttctgttgtaaaggtattttctgtggtcatcagcgacataactttcctttaaagtgcggtcagttttccaggtatttttta Mask Tandem Repeat Region ================================================ taaaaaatacctggaaaactgaccgcactttaaaggaaagttatgtcgctgatgaccacagaaaatacctttacaacagaaatcatcgagcttgttcacaatacgaaacagcgcatggctgtagcggtgaaaaagaagagagaatgatttttaaaataagaagagaaaa Find is-nt database================================================ Query_seq: AA01146:AA01147|AA01146:AA01147:conserved hypothetical protein:nucleotidyltransferase:->->:784831..784999 169 taaaaaatacctggaaaactgaccgcactttaaaggaaagttatgtcgctgatgaccacagaaaatacctttacaacagaaatcatcgagcttgttcacaatacgaaacagcgcatggctgtagcggtgaaaaagaagagagaatgatttttaaaataagaagagaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01146:AA01147|AA01146:AA01147:conserved hypothetical protein:nucleotidyltransferase:->->:784831..784999 169 taaaaaatacctggaaaactgaccgcactttaaaggaaagttatgtcgctgatgaccacagaaaatacctttacaacagaaatcatcgagcttgttcacaatacgaaacagcgcatggctgtagcggtgaaaaagaagagagaatgatttttaaaataagaagagaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01146:AA01147|AA01146:AA01147:conserved hypothetical protein:nucleotidyltransferase:->->:784831..784999 169 taaaaaatacctggaaaactgaccgcactttaaaggaaagttatgtcgctgatgaccacagaaaatacctttacaacagaaatcatcgagcttgttcacaatacgaaacagcgcatggctgtagcggtgaaaaagaagagagaatgatttttaaaataagaagagaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaaatacctggaaaactgaccgcactttaaaggaaagttatgtcgctgatgaccacagaaaatacctttacaacagaaatcatcgagcttgttcacaatacgaaacagcgcatggctgtagcggtgaaaaagaagagagaatgatttttaaaataagaagagaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 34 Strand: + Start: 43 End: 144 .......................................... M S L M T T E N T F T T E I I E L V H N T K Q R M A V A V K K K R E ......................... Protein_Len: 37 Strand: - Start: 1 End: 111 L F Y R S F Q G C K L P F N H R Q H G C F I G K C C F D D L K N V I R F M .......................................................... taaaaaatacctggaaaactgaccgcactttaaaggaaagttatgtcgctgatgaccacagaaaatacctttacaacagaaatcatcgagcttgttcacaatacgaaacagcgcatggctgtagcggtgaaaaagaagagagaatgatttttaaaataagaagagaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaaatacctggaaaactgaccgcactttaaaggaaagttatgtcgctgatgaccacagaaaatacctttacaacagaaatcatcgagcttgttcacaatacgaaacagcgcatggctgtagcggtgaaaaagaagagagaatgatttttaaaataagaagagaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01146:AA01147|AA01146:AA01147:conserved hypothetical protein:nucleotidyltransferase:->->:784831..784999 169 taaaaaatacctggaaaactgaccgcactttaaaggaaagttatgtcgctgatgaccacagaaaatacctttacaacagaaatcatcgagcttgttcacaatacgaaacagcgcatggctgtagcggtgaaaaagaagagagaatgatttttaaaataagaagagaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 169 Len: 169 Subject: aact_AA01146_AA01147|conserved hypothetical protein:nucleotidyltransferase|POSITIVE:POSITIVE|[784831 ,784999]|169 HSP 1 e-value: 3.0E-91 bit: 335.0 Len: 169 Query Start:1 Query End:169 Subject Strand: POSITIVE Subject Start: 1 Subject End: 169 taaaaaatacctggaaaactgaccgcactttaaaggaaagttatgtcgctgatgaccacagaaaatacctttacaacagaaatcatcgagcttgttcacaatacgaaacagcgcatggctgtagcggtgaaaaagaagagagaatgatttttaaaataagaagagaaaa taaaaaatacctggaaaactgaccgcactttaaaggaaagttatgtcgctgatgaccacagaaaatacctttacaacagaaatcatcgagcttgttcacaatacgaaacagcgcatggctgtagcggtgaaaaagaagagagaatgatttttaaaataagaagagaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAAUACCUGGAAAACUGACCGCACUUUAAAGGAAAGUUAUGUCGCUGAUGACCACAGAAAAUACCUUUACAACAGAAAUCAUCGAGCUUGUUCACAAUACGAAACAGCGCAUGGCUGUAGCGGUGAAAAAGAAGAGAGAAUGAUUUUUAAAAUAAGAAGAGAAAA ..........(((...((((..((..........))..))))..((((....))))..))).......(((......((((((((((...(((.(((((....((..(((((.....)))))..)))))))....)))...).))))))))).....)))......... (-28.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUCUCUUCUUAUUUUAAAAAUCAUUCUCUCUUCUUUUUCACCGCUACAGCCAUGCGCUGUUUCGUAUUGUGAACAAGCUCGAUGAUUUCUGUUGUAAAGGUAUUUUCUGUGGUCAUCAGCGACAUAACUUUCCUUUAAAGUGCGGUCAGUUUUCCAGGUAUUUUUUA ...................((((((((.......(((.(((((((..(((((.....)))))..))....))))).)))...))))))))((((..((((((.......(((.((.....)).))).......))))))....))))...................... (-28.14) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 371 50 4 182 136 aact:AA02927|5end_hypothetical_protein_2048233..2048463_POSITIVE 371 50 4 85 39 aact:AA02926|5end_hypothetical_protein_2048136..2048366_POSITIVE 360 31 3 168 140 aact:AA01458|5end_aminotransferase_978820..979050_POSITIVE 356 48 1 143 92 aact:AA00686|5end_hypothetical_protein_468852..469082_POSITIVE 335 32 3 121 92 aact:AA02106|5end_hypothetical_protein_1433241..1433471_POSITIVE 331 50 1 150 99 aact:AA00254|5end_penicillin-binding_protein_5_177579..177809_POSITIVE 330 146 103 50 12 aact:AA02935|5end_high-affinity_zinc_uptake_system_periplasmic_binding_protein_2054343..2054573_POSITIVE 328 49 3 153 108 aact:AA02131|5end_conserved_hypothetical_protein_(possible_TrpH_protein)_1454239..1454469_POSITIVE 325 48 3 152 108 aact:AA01027|5end_Na(+)-translocating_NADH-quinone_reductase_subunit_F_717676..717906_POSITIVE 324 30 2 76 48 aact:AA00952|5end_hemY_protein_homolog_657541..657771_POSITIVE 321 69 21 219 166 aact:AA00212|5end_ribokinase_146056..146286_POSITIVE 321 69 21 118 65 aact:AA00211|5end_hypothetical_protein_145955..146185_POSITIVE 321 59 13 89 31 aact:AA00170|5end_hypothetical_protein_121238..121468_POSITIVE 319 41 1 51 9 aact:AA01041|5end_hypothetical_protein_724853..725083_POSITIVE 318 58 20 202 163 aact:AA03011|5end_inner_membrane_protein,_60_kDa_2102585..2102815_POSITIVE 318 50 2 70 25 aact:AA00697|5end_hypothetical_protein_476999..477229_POSITIVE 316 130 87 214 169 aact:AA02047|5end_galactitol-1-phosphate_5-dehydrogenase_1393633..1393863_POSITIVE 316 50 1 146 91 aact:AA01879|5end_glutamyl-tRNA_reductase_1265717..1265947_POSITIVE 316 48 3 162 122 aact:AA00324|5end_tRNA_(Uracil-5-)-methyltransferase_(tRNA(M-5-U54)-methyltransferase)_220147..220377_POSITIVE 312 129 82 175 123 aact:AA01488|5end_ATP_synthase_F0,_subunit_b_1000676..1000906_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 360 31 3 76 48 aact:AA01457|3end_D-xylose_ABC_transporter,_membrane_spanning_protein_978808..978958_POSITIVE 356 48 1 72 21 aact:AA00684|3end_adenylosuccinate_synthase_468861..469011_POSITIVE 355 48 3 80 33 aact:AA01945|3end_selenophosphate_synthase_1316022..1316172_POSITIVE 345 59 12 112 58 aact:AA01046|3end_conserved_hypothetical_protein_729280..729430_POSITIVE 335 32 3 90 61 aact:AA02105|3end_killing_protein_1433290..1433440_POSITIVE 331 50 1 127 76 aact:AA00253|3end_hypothetical_protein_177636..177786_POSITIVE 330 146 103 44 6 aact:AA02934|3end_hypothetical_protein_2054417..2054567_POSITIVE 328 49 3 135 90 aact:AA02130|3end_hypothetical_protein_1454301..1454451_POSITIVE 328 120 78 69 15 aact:AA01681|3end_molybdate-binding_periplasmic_protein_1135670..1135820_POSITIVE 325 129 84 99 48 aact:AA02601|3end_ATP-dependent_helicase_1813309..1813459_POSITIVE 324 30 2 29 1 aact:AA00950|3end_conserved_hypothetical_protein_657574..657724_POSITIVE 321 69 21 115 62 aact:AA00209|3end_D-ribose_ABC_transporter,_periplasmic-binding_protein_146032..146182_POSITIVE 319 41 1 64 22 aact:AA01040|3end_possible_lipoprotein_724946..725096_POSITIVE 318 58 20 71 32 aact:AA03010|3end_thiophene_and_furan_oxidation_protein_2102534..2102684_POSITIVE 318 50 2 97 52 aact:AA00696|3end_iron(III)_ABC_transporter,_periplasmic_iron-compound-binding_protein_477106..477256_POSITIVE 318 48 4 50 5 aact:AA00592|3end_conserved_hypothetical_protein_(membrane/transport_protein)_407286..407436_POSITIVE 311 143 103 70 35 aact:AA00782|3end_conserved_hypothetical_protein_539120..539270_POSITIVE 308 54 12 69 27 aact:AA00753|3end_galactoside_ABC_transporter,_permease_protein_518615..518765_POSITIVE 306 40 1 43 4 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 304 129 82 147 96 aact:AA02937|3end_conserved_hypothetical_protein_2056947..2057097_POSITIVE