Origin IGS: gagcttttgaaagctggctatctttctttagcagatcatctgctcttactccatttactaaaacatcataagacttttgtcctaatttttcagatttttctttattgaaagaaaaatctattttgtactctttttctaaatttagagatagaggagttagccctttttcgataatattctcaagccctttataatcacatctatgaacttgaattgaagcacttgtcatttcttcttgagttgttttatcaaaacttaattcgtagccagcctcttttgctaattgagctataaagattcgttgtgttcttccgttcccctctctaaaagggtgtatagcgttaagtgcatcgtcctctcgaatataattaagtagctcattgagctgctcttcgttttttttcggctttgcctgaaaaaagttttatatgaccgaatttttgcattgccgcttgctgtggacctctttagcgttatttaatgggatatatgttgtaagatttcagcaggaggggaaattggcatgttatctgctagaaaggtcggcacgcccagtacgtttccaacaacaagcaagatccgcaggtttaccgcttcgctgcattaaggcttatagccttaataattcacccttttaaagtgctcaagaaacaaacagtgaaaagcaaattttcaaccaaagcccataggggatttaatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aattaaatcccctatgggctttggttgaaaatttgcttttcactgtttgtttcttgagcactttaaaagggtgaattattaaggctataagccttaatgcagcgaagcggtaaacctgcggatcttgcttgttgttggaaacgtactgggcgtgccgacctttctagcagataacatgccaatttcccctcctgctgaaatcttacaacatatatcccattaaataacgctaaagaggtccacagcaagcggcaatgcaaaaattcggtcatataaaacttttttcaggcaaagccgaaaaaaaacgaagagcagctcaatgagctacttaattatattcgagaggacgatgcacttaacgctatacacccttttagagaggggaacggaagaacacaacgaatctttatagctcaattagcaaaagaggctggctacgaattaagttttgataaaacaactcaagaagaaatgacaagtgcttcaattcaagttcatagatgtgattataaagggcttgagaatattatcgaaaaagggctaactcctctatctctaaatttagaaaaagagtacaaaatagatttttctttcaataaagaaaaatctgaaaaattaggacaaaagtcttatgatgttttagtaaatggagtaagagcagatgatctgctaaagaaagatagccagctttcaaaagctc Mask Tandem Repeat Region ================================================ gagcttttgaaagctggctatctttctttagcagatcatctgctcttactccatttactaaaacatcataagacttttgtcctaatttttcagatttttctttattgaaagaaaaatctattttgtactctttttctaaatttagagatagaggagttagccctttttcgataatattctcaagccctttataatcacatctatgaacttgaattgaagcacttgtcatttcttcttgagttgttttatcaaaacttaattcgtagccagcctcttttgctaattgagctataaagattcgttgtgttcttccgttcccctctctaaaagggtgtatagcgttaagtgcatcgtcctctcgaatataattaagtagctcattgagctgctcttcgttttttttcggctttgcctgaaaaaagttttatatgaccgaatttttgcattgccgcttgctgtggacctctttagcgttatttaatgggatatatgttgtaagatttcagcaggaggggaaattggcatgttatctgctagaaaggtcggcacgcccagtacgtttccaacaacaagcaagatccgcaggtttaccgcttcgctgcattaaggcttatagccttaataattcacccttttaaagtgctcaagaaacaaacagtgaaaagcaaattttcaaccaaagcccataggggatttaatt Find is-nt database================================================ Query_seq: AA01135:AA01136|AA01135:AA01136:possible cell filamentation protein:hypothetical protein:->->:777725..778424 700 gagcttttgaaagctggctatctttctttagcagatcatctgctcttactccatttactaaaacatcataagacttttgtcctaatttttcagatttttctttattgaaagaaaaatctattttgtactctttttctaaatttagagatagaggagttagccctttttcgataatattctcaagccctttataatcacatctatgaacttgaattgaagcacttgtcatttcttcttgagttgttttatcaaaacttaattcgtagccagcctcttttgctaattgagctataaagattcgttgtgttcttccgttcccctctctaaaagggtgtatagcgttaagtgcatcgtcctctcgaatataattaagtagctcattgagctgctcttcgttttttttcggctttgcctgaaaaaagttttatatgaccgaatttttgcattgccgcttgctgtggacctctttagcgttatttaatgggatatatgttgtaagatttcagcaggaggggaaattggcatgttatctgctagaaaggtcggcacgcccagtacgtttccaacaacaagcaagatccgcaggtttaccgcttcgctgcattaaggcttatagccttaataattcacccttttaaagtgctcaagaaacaaacagtgaaaagcaaattttcaaccaaagcccataggggatttaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01135:AA01136|AA01135:AA01136:possible cell filamentation protein:hypothetical protein:->->:777725..778424 700 gagcttttgaaagctggctatctttctttagcagatcatctgctcttactccatttactaaaacatcataagacttttgtcctaatttttcagatttttctttattgaaagaaaaatctattttgtactctttttctaaatttagagatagaggagttagccctttttcgataatattctcaagccctttataatcacatctatgaacttgaattgaagcacttgtcatttcttcttgagttgttttatcaaaacttaattcgtagccagcctcttttgctaattgagctataaagattcgttgtgttcttccgttcccctctctaaaagggtgtatagcgttaagtgcatcgtcctctcgaatataattaagtagctcattgagctgctcttcgttttttttcggctttgcctgaaaaaagttttatatgaccgaatttttgcattgccgcttgctgtggacctctttagcgttatttaatgggatatatgttgtaagatttcagcaggaggggaaattggcatgttatctgctagaaaggtcggcacgcccagtacgtttccaacaacaagcaagatccgcaggtttaccgcttcgctgcattaaggcttatagccttaataattcacccttttaaagtgctcaagaaacaaacagtgaaaagcaaattttcaaccaaagcccataggggatttaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 1 Min: 164 Max: 346 Len: 183 Subject: gi|89110648|ref|AP_004428.1| stationary-phase protein, cell division [Escherichia coli W3110] HSP 1 e-value: 6.0E-6 bit: 47.8 Len: 183 Query Start:164 Query End:346 Subject Strand: null Subject Start: 131 Subject End: 191 ................................................................................................................................................................... L N A I H P F R E G N G R T Q R I F I A Q L A K E A G Y E L S F D K T T Q E E M T S A S I Q V H R C D Y K G L E N I I E K .................................................................................................................................................................................................................................................................................................................................................................. ................................................................................................................................................................... I N V L H P F R V G S G L A Q R I F F E Q L A I H A G Y Q L S W Q G I E K E A W N Q A N Q S G A M G D L T A L Q M I F S K .................................................................................................................................................................................................................................................................................................................................................................. Find nr database================================================ Query_seq: AA01135:AA01136|AA01135:AA01136:possible cell filamentation protein:hypothetical protein:->->:777725..778424 700 gagcttttgaaagctggctatctttctttagcagatcatctgctcttactccatttactaaaacatcataagacttttgtcctaatttttcagatttttctttattgaaagaaaaatctattttgtactctttttctaaatttagagatagaggagttagcccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgcatcgtcctctcgaatataattaagtagctcattgagctgctcttcgttttttttcggctttgcctgaaaaaagttttatatgaccgaatttttgcattgccgcttgctgtggacctctttagcgttatttaatgggatatatgttgtaagatttcagcaggaggggaaattggcatgttatctgctagaaaggtcggcacgcccagtacgtttccaacaacaagcaagatccgcaggtttaccgcttcgctgcattaaggcttatagccttaataattcacccttttaaagtgctcaagaaacaaacagtgaaaagcaaattttcaaccaaagcccataggggatttaatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 3 Min: 2 Max: 163 Len: 162 Subject: UniRef90_Q8VRE7 Cluster: Hypothetical protein; n=1; Haemophilus influenzae biotype aegyptius|Rep: Hypothetical protein - Haemophilus influenzae biotype aegyptius HSP 1 e-value: 2.0E-20 bit: 102.0 Len: 162 Query Start:2 Query End:163 Subject Strand: null Subject Start: 190 Subject End: 243 . G L T P L S L N L E K E Y K I D F S F N K E K S E K L G Q K S Y D V L V N G V R A D D L L K K D S Q L S K A ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... . G L T P L S L N S E K E Y K I D F S F N K E E S E K L G Q K S Y D V L V N G V R A D N L L K K D S Q L S K A ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q4L0U9 Cluster: Fic-1 cell filamentation-like protein; n=1; Haemophilus influenzae biotype aegyptius|Rep: Fic-1 cell filamentation-like protein - Haemophilus influenzae biotype aegyptius HSP 1 e-value: 3.0E-19 bit: 97.8 Len: 162 Query Start:2 Query End:163 Subject Strand: null Subject Start: 198 Subject End: 251 . G L T P L S L N L E K E Y K I D F S F N K E K S E K L G Q K S Y D V L V N G V R A D D L L K K D S Q L S K A ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... . G L T P L S L N S E N E Y K I D F S F N K E E S E K L G Q K S Y D V L V N G V R A D N L L K K D S Q L S M A ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Subject: UniRef90_Q0I2B3 Cluster: Cell filamentation-like protein; n=1; Haemophilus somnus 129PT|Rep: Cell filamentation-like protein - Haemophilus somnus (strain 129Pt) (Histophilus somni (strain 129Pt)) HSP 1 e-value: 2.0E-12 bit: 75.5 Len: 159 Query Start:2 Query End:160 Subject Strand: null Subject Start: 212 Subject End: 264 . L T P L S L N L E K E Y K I D F S F N K E K S E K L G Q K S Y D V L V N G V R A D D L L K K D S Q L S K A ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ . L S K L S V E Q S K V Y K I E F S F N K E L S E K I G K K S Y D V L V N G I K S D V L L K R D N Q L S K A ............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgcatcgtcctctcgaatataattaagtagctcattgagctgctcttcgttttttttcggctttgcctgaaaaaagttttatatgaccgaatttttgcattgccgcttgctgtggacctctttagcgttatttaatgggatatatgttgtaagatttcagcaggaggggaaattggcatgttatctgctagaaaggtcggcacgcccagtacgtttccaacaacaagcaagatccgcaggtttaccgcttcgctgcattaaggcttatagccttaataattcacccttttaaagtgctcaagaaacaaacagtgaaaagcaaattttcaaccaaagcccataggggatttaatt Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 7 End: 105 ...... M K A G Y L S L A D H L L L L H L L K H H K T F V L I F Q I F L Y ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 31 Strand: + Start: 199 End: 291 ...................................................................................................................................................................................................... M Y E L E L K H L S F L L E L F Y Q N L I R S Q P L L L I E L ......................................................................................................................................................................................................................................................................................................................................................................................................................... Protein_Len: 30 Strand: + Start: 284 End: 373 ........................................................................................................................................................................................................................................................................................... M S Y K D S L C S S V P L S K R V Y S V K C I V L S N I I K ....................................................................................................................................................................................................................................................................................................................................... Protein_Len: 33 Strand: + Start: 380 End: 478 ........................................................................................................................................................................................................................................................................................................................................................................................... M E L L F V F F R L C L K K V L Y D R I F A L P L A V D L F S V I .............................................................................................................................................................................................................................. Protein_Len: 39 Strand: + Start: 488 End: 604 ....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... M C C K I S A G G E I G M L S A R K V G T P S T F P T T S K I R R F T A S L H ................................................................................................ Protein_Len: 60 Strand: + Start: 429 End: 635 ............................................................................................................................................................................................................................................................................................................................................................................................................................................ M T E F L H C R L L W T S L A L F N G I Y V V R F Q Q E G K L A C Y L L E R S A R P V R F Q Q Q A R S A G L P L R C I K ................................................................. Protein_Len: 35 Strand: - Start: 481 End: 585 ................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................ H S I H Q L I E A P P S I P M N D A L F T P V G L V N G V V L L I R M ................................................................................................................... Protein_Len: 60 Strand: - Start: 292 End: 477 ................................................................................................................................................................................................................................................................................................... E N H E E T G R E L L T Y L T L H M T R E F I I L Y S M S S S K T K K R S Q R F F T K Y S R I K A N G S A T S R K L T M ............................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 2 End: 364 . G K Y D C R H V Q I S A S T M E E Q T T K D F S L E Y G A E K A L Q A I F I R Q T R G N G E R F P H I A N L A D D E R M ................................................................................................................................................................................................................................................................................................................................................ gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgcatcgtcctctcgaatataattaagtagctcattgagctgctcttcgttttttttcggctttgcctgaaaaaagttttatatgaccgaatttttgcattgccgcttgctgtggacctctttagcgttatttaatgggatatatgttgtaagatttcagcaggaggggaaattggcatgttatctgctagaaaggtcggcacgcccagtacgtttccaacaacaagcaagatccgcaggtttaccgcttcgctgcattaaggcttatagccttaataattcacccttttaaagtgctcaagaaacaaacagtgaaaagcaaattttcaaccaaagcccataggggatttaatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 81 Start: 65 End: 94 ................................................................TCTGAAAAATTAGGACAAAAGTCTTATGAT.............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................. PromScan Matrix: RpoD-17 Strand: + Score: 81 Start: 166 End: 195 .....................................................................................................................................................................TTTCGATAATATTCTCAAGCCCTTTATAAT......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ PromScan Matrix: RpoN Strand: + Score: 83 Start: 519 End: 535 ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTGGCATGTTATCTGCT..................................................................................................................................................................... gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgcatcgtcctctcgaatataattaagtagctcattgagctgctcttcgttttttttcggctttgcctgaaaaaagttttatatgaccgaatttttgcattgccgcttgctgtggacctctttagcgttatttaatgggatatatgttgtaagatttcagcaggaggggaaattggcatgttatctgctagaaaggtcggcacgcccagtacgtttccaacaacaagcaagatccgcaggtttaccgcttcgctgcattaaggcttatagccttaataattcacccttttaaagtgctcaagaaacaaacagtgaaaagcaaattttcaaccaaagcccataggggatttaatt Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 88 HP_score: -8.1 Tail_Score: -4.62069 Start: 91 End: 120 Full_Region: TTTGTCCTAATTTTT CAGATTTTTCTTT ATTG AAAGAAAAATCTA TTTTGTACTCTTTTT ..........................................................................................CAGATTTTTCTTTATTGAAAGAAAAATCTA.................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 88 HP_score: -10.7 Tail_Score: -3.88881 Start: 93 End: 118 Full_Region: TGTCCTAATTTTTCA GATTTTTCTTT ATTG AAAGAAAAATC TATTTTGTACTCTTT ............................................................................................GATTTTTCTTTATTGAAAGAAAAATC...................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 75 HP_score: -8.7 Tail_Score: -3.6311 Start: 373 End: 389 Full_Region: CTCGAATATAATTAA GTAGCTC ATT GAGCTGC TCTTCGTTTTTTTTC ....................................................................................................................................................................................................................................................................................................................................................................................GTAGCTCATTGAGCTGC....................................................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 73 HP_score: -6.3 Tail_Score: -4.52589 Start: 397 End: 422 Full_Region: TGAGCTGCTCTTCGT TTTTTTTCGGC TTT GCCTGAAAAAAG TTTTATATGACCGAA ............................................................................................................................................................................................................................................................................................................................................................................................................TTTTTTTCGGCTTTGCCTGAAAAAAG...................................................................................................................................................................................................................................................................................... TransTerm Strand: + Conf: 76 HP_score: -10.4 Tail_Score: -2.75229 Start: 604 End: 622 Full_Region: TACCGCTTCGCTGCA TTAAGGCT TAT AGCCTTAA TAATTCACCCTTTTA ...........................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................TTAAGGCTTATAGCCTTAA.............................................................................. Find igs database================================================ Query_seq: AA01135:AA01136|AA01135:AA01136:possible cell filamentation protein:hypothetical protein:->->:777725..778424 700 gnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgcatcgtcctctcgaatataattaagtagctcattgagctgctcttcgttttttttcggctttgcctgaaaaaagttttatatgaccgaatttttgcattgccgcttgctgtggacctctttagcgttatttaatgggatatatgttgtaagatttcagcaggaggggaaattggcatgttatctgctagaaaggtcggcacgcccagtacgtttccaacaacaagcaagatccgcaggtttaccgcttcgctgcattaaggcttatagccttaataattcacccttttaaagtgctcaagaaacaaacagtgaaaagcaaattttcaaccaaagcccataggggatttaatt Intra-Species Hit: Count: 1 Min: 347 Max: 700 Len: 354 Subject: aact_AA01135_AA01136|possible cell filamentation protein:hypothetical protein|POSITIVE:POSITIVE|[777725,778424]|700 HSP 1 e-value: 0.0 bit: 654.0 Len: 354 Query Start:347 Query End:700 Subject Strand: POSITIVE Subject Start: 347 Subject End: 700 ..........................................................................................................................................................................................................................................................................................................................................................tgcatcgtcctctcgaatataattaagtagctcattgagctgctcttcgnnnnnnnncggctttgcctgaaaaaagttttatatgaccgaatttttgcattgccgcttgctgtggacctctttagcgttatttaatgggatatatgttgtaagatttcagcaggaggggaaattggcatgttatctgctagaaaggtcggcacgcccagtacgtttccaacaacaagcaagatccgcaggtttaccgcttcgctgcattaaggcttatagccttaataattcacccttttaaagtgctcaagaaacaaacagtgaaaagcaaattttcaaccaaagcccataggggatttaatt ..........................................................................................................................................................................................................................................................................................................................................................tgcatcgtcctctcgaatataattaagtagctcattgagctgctcttcgttttttttcggctttgcctgaaaaaagttttatatgaccgaatttttgcattgccgcttgctgtggacctctttagcgttatttaatgggatatatgttgtaagatttcagcaggaggggaaattggcatgttatctgctagaaaggtcggcacgcccagtacgtttccaacaacaagcaagatccgcaggtttaccgcttcgctgcattaaggcttatagccttaataattcacccttttaaagtgctcaagaaacaaacagtgaaaagcaaattttcaaccaaagcccataggggatttaatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGCAUCGUCCUCUCGAAUAUAAUUAAGUAGCUCAUUGAGCUGCUCUUCGUUUUUUUUCGGCUUUGCCUGAAAAAAGUUUUAUAUGACCGAAUUUUUGCAUUGCCGCUUGCUGUGGACCUCUUUAGCGUUAUUUAAUGGGAUAUAUGUUGUAAGAUUUCAGCAGGAGGGGAAAUUGGCAUGUUAUCUGCUAGAAAGGUCGGCACGCCCAGUACGUUUCCAACAACAAGCAAGAUCCGCAGGUUUACCGCUUCGCUGCAUUAAGGCUUAUAGCCUUAAUAAUUCACCCUUUUAAAGUGCUCAAGAAACAAACAGUGAAAAGCAAAUUUUCAACCAAAGCCCAUAGGGGAUUUAAUU ......(((((((............((((((((...))))))))..............(((((((..((((((...........((((....((((((....((.(((((((......(((((((((((((((....))))).)))))).))))...))))))).))((((((.(((.((((((((.......)))).)))).)))......))))))........))))))......))))....(((((((((.((((((((.....))))))))....(((.........))).............))))))..)))...))))))..)))))))...)))))))...... (-93.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUUAAAUCCCCUAUGGGCUUUGGUUGAAAAUUUGCUUUUCACUGUUUGUUUCUUGAGCACUUUAAAAGGGUGAAUUAUUAAGGCUAUAAGCCUUAAUGCAGCGAAGCGGUAAACCUGCGGAUCUUGCUUGUUGUUGGAAACGUACUGGGCGUGCCGACCUUUCUAGCAGAUAACAUGCCAAUUUCCCCUCCUGCUGAAAUCUUACAACAUAUAUCCCAUUAAAUAACGCUAAAGAGGUCCACAGCAAGCGGCAAUGCAAAAAUUCGGUCAUAUAAAACUUUUUUCAGGCAAAGCCGAAAAAAAACGAAGAGCAGCUCAAUGAGCUACUUAAUUAUAUUCGAGAGGACGAUGCA ......(((.(((...(((((((.((((((((((((......(((((((((((((.(((..(((((..(((((...(((((((((.....)))))))))(((((.((.((.(((...(((.((((.(((.(..(((((..((((.....)))).)))))....).)))))))....)))...))).))))..)))))...............)))))..)))))....))).)))).......)))))))))....))))).....(((........))).))))))).))))))).........(((((((.(((((...))))).))).......))))..)))..)))... (-86.92) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 487 217 172 54 11 aact:AA01996|5end_prephenate_dehydrogenase_1355314..1355544_POSITIVE 395 255 212 167 127 aact:AA00576|5end_conserved_hypothetical_protein_(possible_translation_factor)_394020..394250_POSITIVE 364 290 244 192 141 aact:AA02888|5end_ssrA-binding_protein,_small_protein_B_2024628..2024858_POSITIVE 360 140 91 83 37 aact:AA01760|5end_hydrogenase_isoenzymes_formation_protein_1189787..1190017_POSITIVE 359 127 88 73 34 aact:AA00884|5end_sodium-dependent_transporter_614392..614622_POSITIVE 354 275 236 225 184 aact:AA00410|5end_peptide_deformylase_(PDF)_(Polypeptide_deformylase)_274633..274863_POSITIVE 354 199 158 74 35 aact:AA00198|5end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138039..138269_POSITIVE 340 69 30 48 8 aact:AA02153|5end_iron(III)_ABC_transporter,_permease_protein_1472530..1472760_POSITIVE 339 240 193 208 163 aact:AA02368|5end_conserved_hypothetical_protein_1623544..1623774_POSITIVE 335 240 198 194 146 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 335 206 162 229 185 aact:AA00305|5end_conserved_hypothetical_protein_206741..206971_POSITIVE 333 219 171 129 60 aact:AA02316|5end_exodeoxyribonuclease_VII_large_subunit_1581682..1581912_POSITIVE 332 209 161 229 170 aact:AA00327|5end_conserved_hypothetical_protein_222339..222569_POSITIVE 329 270 232 41 1 aact:AA02683|5end_hydrogenase_4_Fe-S_subunit_1868882..1869112_POSITIVE 326 200 151 75 24 aact:AA02359|5end_hypothetical_protein_1618100..1618330_POSITIVE 326 130 83 173 126 aact:AA01519|5end_conserved_hypothetical_protein_1021794..1022024_POSITIVE 325 206 166 180 141 aact:AA01429|5end_conserved_hypothetical_protein_959581..959811_POSITIVE 324 209 161 175 125 aact:AA00161|5end_pilin_B_biogenesis_protein;_fimbrial_transcription_regulation_113384..113614_POSITIVE 323 130 82 77 22 aact:AA02099|5end_50S_ribosomal_protein_L25_1429639..1429869_POSITIVE 321 256 212 195 149 aact:AA00896|5end_prolyl-tRNA_synthetase_(proline-tRNA_ligase)_621414..621644_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 487 217 172 54 11 aact:AA01995|3end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355394..1355544_POSITIVE 364 290 244 118 67 aact:AA02886|3end_OBG-related_GTP-binding_protein_2024634..2024784_POSITIVE 360 140 91 80 34 aact:AA01759|3end_hydrogenase_isoenzymes_formation_protein_1189864..1190014_POSITIVE 359 127 88 68 29 aact:AA00883|3end_hypothetical_protein_614467..614617_POSITIVE 340 69 30 48 8 aact:AA02152|3end_ferric_enterobactin_transport;_ABC_transporter_ATP-binding_protein_1472610..1472760_POSITIVE 338 248 202 150 113 aact:AA00575|3end_shikimate_5-dehydrogenase_394093..394243_POSITIVE 333 219 171 119 50 aact:AA02315|3end_conserved_hypothetical_protein_1581752..1581902_POSITIVE 326 130 83 90 43 aact:AA01517|3end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1021791..1021941_POSITIVE 325 206 166 144 105 aact:AA01428|3end_acetolactate_synthase_959625..959775_POSITIVE 324 209 161 145 95 aact:AA00160|3end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_113434..113584_POSITIVE 321 256 212 51 5 aact:AA00894|3end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_621350..621500_POSITIVE 320 200 151 87 38 aact:AA01041|3end_hypothetical_protein_725055..725205_POSITIVE 319 129 87 78 29 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 319 220 174 60 11 aact:AA00344|3end_preprotein_translocase_secE_subunit_231505..231655_POSITIVE 319 238 195 68 16 aact:AA00128|3end_cation-transporting_ATPase_(probable_potassium/copper_transporter)_94207..94357_POSITIVE 316 266 229 142 106 aact:AA01781|3end_molybdenum_ABC_transporter,_periplasmic_substrate-binding_1202732..1202882_POSITIVE 313 237 195 123 82 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 311 264 221 141 98 aact:AA01417|3end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_954028..954178_POSITIVE 309 207 177 147 109 aact:AA02978|3end_sufI_protein_precursor_homolog_2083308..2083458_POSITIVE 306 210 173 44 10 aact:AA01087|3end_DNA_polymerase_III,_delta_subunit_752980..753130_POSITIVE