Origin IGS: aaagaatttttttaaaagtctttcacaagccttttataaatcttttacatattatttatagaaaaaaataaaaaacgagcaaaataactaccgctttcctacggttgttgctgaattctccaaagagtaaagacggactacgttcgtttttttactctttagactattattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaataatagtctaaagagtaaaaaaacgaacgtagtccgtctttactctttggagaattcagcaacaaccgtaggaaagcggtagttattttgctcgttttttatttttttctataaataatatgtaaaagatttataaaaggcttgtgaaagacttttaaaaaaattcttt Mask Tandem Repeat Region ================================================ aaagaatttttttaaaagtctttcacaagccttttataaatcttttacatattatttatagaaaaaaataaaaaacgagcaaaataactaccgctttcctacggttgttgctgaattctccaaagagtaaagacggactacgttcgtttttttactctttagactattattta Find is-nt database================================================ Query_seq: AA01134:AA01135|AA01134:AA01135:conserved hypothetical protein:possible cell filamentation protein:->->:777348..777520 173 aaagaatttttttaaaagtctttcacaagccttttataaatcttttacatattatttatagaaaaaaataaaaaacgagcaaaataactaccgctttcctacggttgttgctgaattctccaaagagtaaagacggactacgttcgtttttttactctttagactattattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01134:AA01135|AA01134:AA01135:conserved hypothetical protein:possible cell filamentation protein:->->:777348..777520 173 aaagaatttttttaaaagtctttcacaagccttttataaatcttttacatattatttatagaaaaaaataaaaaacgagcaaaataactaccgctttcctacggttgttgctgaattctccaaagagtaaagacggactacgttcgtttttttactctttagactattattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01134:AA01135|AA01134:AA01135:conserved hypothetical protein:possible cell filamentation protein:->->:777348..777520 173 aaagaatttttttaaaagtctttcacaagccttttataaatcttttacatattatttatagaaaaaaataaaaaacgagcaaaataactaccgctttcctacggttgttgctgaattctccaaagagtaaagacggactacgttcgtttttttactctttagactattattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aaagaatttttttaaaagtctttcacaagccttttataaatcttttacatattatttatagaaaaaaataaaaaacgagcaaaataactaccgctttcctacggttgttgctgaattctccaaagagtaaagacggactacgttcgtttttttactctttagactattattta Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 84 End: 173 ................................................................................... M T T A F L R L L L N S P K S K D G L R S F F Y S L D Y Y L aaagaatttttttaaaagtctttcacaagccttttataaatcttttacatattatttatagaaaaaaataaaaaacgagcaaaataactaccgctttcctacggttgttgctgaattctccaaagagtaaagacggactacgttcgtttttttactctttagactattattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aaagaatttttttaaaagtctttcacaagccttttataaatcttttacatattatttatagaaaaaaataaaaaacgagcaaaataactaccgctttcctacggttgttgctgaattctccaaagagtaaagacggactacgttcgtttttttactctttagactattattta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 93 HP_score: -5.5 Tail_Score: -5.66989 Start: 134 End: 146 Full_Region: TCCAAAGAGTAAAGA CGGAC TAC GTTCG TTTTTTTACTCTTTA .....................................................................................................................................CGGACTACGTTCG........................... Find igs database================================================ Query_seq: AA01134:AA01135|AA01134:AA01135:conserved hypothetical protein:possible cell filamentation protein:->->:777348..777520 173 aaagaatttttttaaaagtctttcacaagccttttataaatcttttacatattatttatagaaaaaaataaaaaacgagcaaaataactaccgctttcctacggttgttgctgaattctccaaagagtaaagacggactacgttcgtttttttactctttagactattattta Intra-Species Hit: Count: 1 Min: 14 Max: 173 Len: 160 Subject: aact_AA01134_AA01135|conserved hypothetical protein:possible cell filamentation protein|POSITIVE:POSITIVE|[777348,777520]|173 HSP 1 e-value: 2.0E-36 bit: 153.0 Len: 98 Query Start:76 Query End:173 Subject Strand: POSITIVE Subject Start: 76 Subject End: 173 ...........................................................................cgagcaaaataactaccgctttcctacggttgttgctgaattctccaaagagtaaagacggactacgttcgnnnnnnnactctttagactattattta ...........................................................................cgagcaaaataactaccgctttcctacggttgttgctgaattctccaaagagtaaagacggactacgttcgtttttttactctttagactattattta HSP 2 e-value: 5.0E-19 bit: 95.6 Len: 48 Query Start:14 Query End:61 Subject Strand: POSITIVE Subject Start: 14 Subject End: 61 .............aaaagtctttcacaagccttttataaatcttttacatattatttatag................................................................................................................ .............aaaagtctttcacaagccttttataaatcttttacatattatttatag................................................................................................................ Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAAAGUCUUUCACAAGCCUUUUAUAAAUCUUUUACAUAUUAUUUAUAGAAAAAAAUAAAAAACGAGCAAAAUAACUACCGCUUUCCUACGGUUGUUGCUGAAUUCUCCAAAGAGUAAAGACGGACUACGUUCGUUUUUUUACUCUUUAGACUAUUAUUUA ...((((.(((.......((((((((((..(......)..))))))))))..............(((((.......((((........))))..))))))))......(((((((((((((((((...)))))))..)))))))))).))))........ (-31.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUAAUAGUCUAAAGAGUAAAAAAACGAACGUAGUCCGUCUUUACUCUUUGGAGAAUUCAGCAACAACCGUAGGAAAGCGGUAGUUAUUUUGCUCGUUUUUUAUUUUUUUCUAUAAAUAAUAUGUAAAAGAUUUAUAAAAGGCUUGUGAAAGACUUUU ..........(((((((((((((...(((.((...)).))).))))))))))))).........(((.(((((......))))).))).(((..(..(((((((((..((((.((((.......)))).))))...)))))))))..)..)))....... (-34.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 326 130 81 196 148 aact:AA01566|5end_conserved_hypothetical_protein_1051811..1052041_POSITIVE 326 130 81 196 148 aact:AA01261|5end_conserved_hypothetical_protein_856443..856673_POSITIVE 284 109 64 165 117 aact:AA02062|5end_conserved_hypothetical_protein_(probable_membrane_protein)_1403427..1403657_POSITIVE 284 102 78 73 43 aact:AA00636|5end_MrsA_protein_(phosphoglucomutase/phosphomannomutase)_436780..437010_POSITIVE 269 109 62 202 149 aact:AA02575|5end_transmembrane_protein_1793749..1793979_POSITIVE 266 139 91 129 76 aact:AA00649|5end_peptide_chain_release_factor_1_(RF-1)_443277..443507_POSITIVE 265 108 63 49 1 aact:AA02966|5end_conserved_hypothetical_protein_2074223..2074453_POSITIVE 263 132 93 154 108 aact:AA01460|5end_hypothetical_protein_981412..981642_POSITIVE 262 114 71 185 145 aact:AA02045|5end_PTS_system,_galactitol-specific_IIB_component_1391956..1392186_POSITIVE 262 108 75 200 164 aact:AA00416|5end_D-methionine_transport_system_permease_protein_(ABC_transport_system)_282677..282907_POSITIVE 258 97 58 143 98 aact:AA00757|5end_aerobic_respiration_sensor-response_protein_518615..518845_POSITIVE 256 100 63 52 7 aact:AA02351|5end_conserved_hypothetical_protein_1609233..1609463_POSITIVE 255 98 54 135 90 aact:AA01187|5end_conserved_hypothetical_protein_813331..813561_POSITIVE 253 109 75 157 128 aact:AA02196|5end_oxaloacetate_decarboxylase_beta_chain_1496308..1496538_POSITIVE 252 108 63 46 3 aact:AA01755|5end_cobalt_membrane_transport_protein_1185825..1186055_POSITIVE 248 114 79 95 59 aact:AA01270|5end_hydrolase_(possible_2-haloalkanoic_acid_dehalogenase_I)_863317..863547_POSITIVE 247 135 93 179 125 aact:AA01823|5end_hypothetical_protein_1227563..1227793_POSITIVE 246 94 56 49 8 aact:AA01660|5end_hypothetical_protein_1125035..1125265_POSITIVE 245 100 57 227 171 aact:AA02944|5end_30S_ribosomal_protein_S20_2058688..2058918_POSITIVE 245 100 57 74 18 aact:AA02941|5end_hypothetical_protein_2058535..2058765_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 287 109 77 45 3 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE 287 109 77 45 3 aact:AA01261|3end_conserved_hypothetical_protein_856723..856873_POSITIVE 284 109 64 148 100 aact:AA02060|3end_cardiolipin_synthase_1403490..1403640_POSITIVE 284 102 78 41 11 aact:AA00635|3end_dihydropteroate_synthase_436828..436978_POSITIVE 274 110 66 65 9 aact:AA00809|3end_possible_oxidoreductase_555435..555585_POSITIVE 266 139 91 86 33 aact:AA00647|3end_protoporphyrinogen_oxidase_443314..443464_POSITIVE 258 97 58 63 18 aact:AA00753|3end_galactoside_ABC_transporter,_permease_protein_518615..518765_POSITIVE 255 98 54 125 80 aact:AA01185|3end_conserved_hypothetical_protein_(possible_membrane_protein)_813401..813551_POSITIVE 253 109 75 143 114 aact:AA02195|3end_oxaloacetate_decarboxylase_alpha_chain_1496374..1496524_POSITIVE 248 114 79 111 75 aact:AA01269|3end_hypothetical_protein_863413..863563_POSITIVE 245 100 57 111 55 aact:AA02940|3end_conserved_hypothetical_protein_2058652..2058802_POSITIVE 244 138 95 66 20 aact:AA02554|3end_conserved_hypothetical_protein_1780486..1780636_POSITIVE 242 109 65 109 58 aact:AA01472|3end_hypothetical_protein_989158..989308_POSITIVE 238 129 82 151 95 aact:AA02328|3end_anti-sigma_factor_B_antagonist_1589736..1589886_POSITIVE 233 150 103 68 23 aact:AA00429|3end_conserved_hypothetical_protein_291891..292041_POSITIVE 232 138 97 95 53 aact:AA02551|3end_iron_(chelated)_ABC_transporter,_permease_protein_1778575..1778725_POSITIVE 230 100 62 137 94 aact:AA01151|3end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_789422..789572_POSITIVE 228 114 71 142 106 aact:AA02538|3end_conserved_hypothetical_protein_1765992..1766142_POSITIVE 228 129 90 100 67 aact:AA00205|3end_ribose_ABC_transporter_protein_/_high_affinity_D-ribose_transport_protein_142658..142808_POSITIVE 226 91 56 83 45 aact:AA01611|3end_anthranilate_synthase_component_II_1089619..1089769_POSITIVE