Origin IGS: tagaatacttaatagcattttacggagcttatctttgaagcattgcaggtgcggcagatgaacaggtcagtattaaattctaccatacttgctattcttgcgtaatcttaatggtctcatttaaggttgccatcttgcctttaaaaatcgccaattggcgtaaaatttgtttacgttcaagttttaaactatttaacttttgctgaatttgattggtgtagattttttgtatgctactagtgtaatactatctcttggggaatgtgtttacgttgctagggaatgcgccattcattttgttgattagttgtattcaggcatttattcaatgcattaactgccagctgccagattgtcagcgattatcttcacgcaacaaacgccgccacttttatctcacaggcttcaagcggcgaaattaaacaagagaaaccgaaaaaaataagcactaaatttatctacggtaagatttacagaagctcaatataga .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tctatattgagcttctgtaaatcttaccgtagataaatttagtgcttatttttttcggtttctcttgtttaatttcgccgcttgaagcctgtgagataaaagtggcggcgtttgttgcgtgaagataatcgctgacaatctggcagctggcagttaatgcattgaataaatgcctgaatacaactaatcaacaaaatgaatggcgcattccctagcaacgtaaacacattccccaagagatagtattacactagtagcatacaaaaaatctacaccaatcaaattcagcaaaagttaaatagtttaaaacttgaacgtaaacaaattttacgccaattggcgatttttaaaggcaagatggcaaccttaaatgagaccattaagattacgcaagaatagcaagtatggtagaatttaatactgacctgttcatctgccgcacctgcaatgcttcaaagataagctccgtaaaatgctattaagtattcta Mask Tandem Repeat Region ================================================ tagaatacttaatagcattttacggagcttatctttgaagcattgcaggtgcggcagatgaacaggtcagtattaaattctaccatacttgctattcttgcgtaatcttaatggtctcatttaaggttgccatcttgcctttaaaaatcgccaattggcgtaaaatttgtttacgttcaagttttaaactatttaacttttgctgaatttgattggtgtagattttttgtatgctactagtgtaatactatctcttggggaatgtgtttacgttgctagggaatgcgccattcattttgttgattagttgtattcaggcatttattcaatgcattaactgccagctgccagattgtcagcgattatcttcacgcaacaaacgccgccacttttatctcacaggcttcaagcggcgaaattaaacaagagaaaccgaaaaaaataagcactaaatttatctacggtaagatttacagaagctcaatataga Find is-nt database================================================ Query_seq: AA01128:AA01129|AA01128:AA01129:hypothetical protein:hypothetical protein:->->:774263..774752 490 tagaatacttaatagcattttacggagcttatctttgaagcattgcaggtgcggcagatgaacaggtcagtattaaattctaccatacttgctattcttgcgtaatcttaatggtctcatttaaggttgccatcttgcctttaaaaatcgccaattggcgtaaaatttgtttacgttcaagttttaaactatttaacttttgctgaatttgattggtgtagattttttgtatgctactagtgtaatactatctcttggggaatgtgtttacgttgctagggaatgcgccattcattttgttgattagttgtattcaggcatttattcaatgcattaactgccagctgccagattgtcagcgattatcttcacgcaacaaacgccgccacttttatctcacaggcttcaagcggcgaaattaaacaagagaaaccgaaaaaaataagcactaaatttatctacggtaagatttacagaagctcaatataga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01128:AA01129|AA01128:AA01129:hypothetical protein:hypothetical protein:->->:774263..774752 490 tagaatacttaatagcattttacggagcttatctttgaagcattgcaggtgcggcagatgaacaggtcagtattaaattctaccatacttgctattcttgcgtaatcttaatggtctcatttaaggttgccatcttgcctttaaaaatcgccaattggcgtaaaatttgtttacgttcaagttttaaactatttaacttttgctgaatttgattggtgtagattttttgtatgctactagtgtaatactatctcttggggaatgtgtttacgttgctagggaatgcgccattcattttgttgattagttgtattcaggcatttattcaatgcattaactgccagctgccagattgtcagcgattatcttcacgcaacaaacgccgccacttttatctcacaggcttcaagcggcgaaattaaacaagagaaaccgaaaaaaataagcactaaatttatctacggtaagatttacagaagctcaatataga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01128:AA01129|AA01128:AA01129:hypothetical protein:hypothetical protein:->->:774263..774752 490 tagaatacttaatagcattttacggagcttatctttgaagcattgcaggtgcggcagatgaacaggtcagtattaaattctaccatacttgctattcttgcgtaatcttaatggtctcatttaaggttgccatcttgcctttaaaaatcgccaattggcgtaaaatttgtttacgttcaagttttaaactatttaacttttgctgaatttgattggtgtagattttttgtatgctactagtgtaatactatctcttggggaatgtgtttacgttgctagggaatgcgccattcattttgttgattagttgtattcaggcatttattcaatgcattaactgccagctgccagattgtcagcgattatcttcacgcaacaaacgccgccacttttatctcacaggcttcaagcggcgaaattaaacaagagaaaccgaaaaaaataagcactaaatttatctacggtaagatttacagaagctcaatataga Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tagaatacttaatagcattttacggagcttatctttgaagcattgcaggtgcggcagatgaacaggtcagtattaaattctaccatacttgctattcttgcgtaatcttaatggtctcatttaaggttgccatcttgcctttaaaaatcgccaattggcgtaaaatttgtttacgttcaagttttaaactatttaacttttgctgaatttgattggtgtagattttttgtatgctactagtgtaatactatctcttggggaatgtgtttacgttgctagggaatgcgccattcattttgttgattagttgtattcaggcatttattcaatgcattaactgccagctgccagattgtcagcgattatcttcacgcaacaaacgccgccacttttatctcacaggcttcaagcggcgaaattaaacaagagaaaccgaaaaaaataagcactaaatttatctacggtaagatttacagaagctcaatataga Predict ORF larger than 30AA ================================================ Protein_Len: 54 Strand: + Start: 42 End: 203 ......................................... M A G A A D E Q V S I K F Y H T C Y S C V I L M V S F K V A I L P L K I A N W R K I C L R S S F K L F N F C ............................................................................................................................................................................................................................................................................................... Protein_Len: 35 Strand: + Start: 200 End: 304 ....................................................................................................................................................................................................... M L N L I G V D F L Y A T S V I L S L G E C V Y V A R E C A I H F V D .......................................................................................................................................................................................... Protein_Len: 55 Strand: + Start: 255 End: 419 .............................................................................................................................................................................................................................................................. M G N V F T L L G N A P F I L L I S C I Q A F I Q C I N C Q L P D C Q R L S S R N K R R H F Y L T G F K R R N ....................................................................... Protein_Len: 35 Strand: + Start: 338 End: 442 ................................................................................................................................................................................................................................................................................................................................................. M P A A R L S A I I F T Q Q T P P L L S H R L Q A A K L N K R N R K K ................................................ Protein_Len: 46 Strand: + Start: 352 End: 486 ............................................................................................................................................................................................................................................................................................................................................................... M V S D Y L H A T N A A T F I S Q A S S G E I K Q E K P K K I S T K F I Y G K I Y R S S I * .... Protein_Len: 46 Strand: - Start: 95 End: 232 .............................................................................................. E Q T I K I T E N L T A M K G K F I A L Q R L I Q K R E L K L S N L K Q Q I Q N T Y I K Q M .................................................................................................................................................................................................................................................................. tagaatacttaatagcattttacggagcttatctttgaagcattgcaggtgcggcagatgaacaggtcagtattaaattctaccatacttgctattcttgcgtaatcttaatggtctcatttaaggttgccatcttgcctttaaaaatcgccaattggcgtaaaatttgtttacgttcaagttttaaactatttaacttttgctgaatttgattggtgtagattttttgtatgctactagtgtaatactatctcttggggaatgtgtttacgttgctagggaatgcgccattcattttgttgattagttgtattcaggcatttattcaatgcattaactgccagctgccagattgtcagcgattatcttcacgcaacaaacgccgccacttttatctcacaggcttcaagcggcgaaattaaacaagagaaaccgaaaaaaataagcactaaatttatctacggtaagatttacagaagctcaatataga Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tagaatacttaatagcattttacggagcttatctttgaagcattgcaggtgcggcagatgaacaggtcagtattaaattctaccatacttgctattcttgcgtaatcttaatggtctcatttaaggttgccatcttgcctttaaaaatcgccaattggcgtaaaatttgtttacgttcaagttttaaactatttaacttttgctgaatttgattggtgtagattttttgtatgctactagtgtaatactatctcttggggaatgtgtttacgttgctagggaatgcgccattcattttgttgattagttgtattcaggcatttattcaatgcattaactgccagctgccagattgtcagcgattatcttcacgcaacaaacgccgccacttttatctcacaggcttcaagcggcgaaattaaacaagagaaaccgaaaaaaataagcactaaatttatctacggtaagatttacagaagctcaatataga Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 43 HP_score: -2.0 Tail_Score: -3.12373 Start: 155 End: 180 Full_Region: TTTAAAAATCGCCAA TTGGCGTAAA ATTTG TTTACGTTCAA GTTTTAAACTATTTA ..........................................................................................................................................................TTGGCGTAAAATTTGTTTACGTTCAA...................................................................................................................................................................................................................................................................................................................... Find igs database================================================ Query_seq: AA01128:AA01129|AA01128:AA01129:hypothetical protein:hypothetical protein:->->:774263..774752 490 tagaatacttaatagcattttacggagcttatctttgaagcattgcaggtgcggcagatgaacaggtcagtattaaattctaccatacttgctattcttgcgtaatcttaatggtctcatttaaggttgccatcttgcctttaaaaatcgccaattggcgtaaaatttgtttacgttcaagttttaaactatttaacttttgctgaatttgattggtgtagattttttgtatgctactagtgtaatactatctcttggggaatgtgtttacgttgctagggaatgcgccattcattttgttgattagttgtattcaggcatttattcaatgcattaactgccagctgccagattgtcagcgattatcttcacgcaacaaacgccgccacttttatctcacaggcttcaagcggcgaaattaaacaagagaaaccgaaaaaaataagcactaaatttatctacggtaagatttacagaagctcaatataga Intra-Species Hit: Count: 1 Min: 1 Max: 490 Len: 490 Subject: aact_AA01128_AA01129|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[774263,774752]|490 HSP 1 e-value: 0.0 bit: 930.0 Len: 490 Query Start:1 Query End:490 Subject Strand: POSITIVE Subject Start: 1 Subject End: 490 tagaatacttaatagcattttacggagcttatctttgaagcattgcaggtgcggcagatgaacaggtcagtattaaattctaccatacttgctattcttgcgtaatcttaatggtctcatttaaggttgccatcttgcctttaaaaatcgccaattggcgtaaaatttgtttacgttcaagttttaaactatttaacttttgctgaatttgattggtgtagattttttgtatgctactagtgtaatactatctcttggggaatgtgtttacgttgctagggaatgcgccattcattttgttgattagttgtattcaggcatttattcaatgcattaactgccagctgccagattgtcagcgattatcttcacgcaacaaacgccgccacttttatctcacaggcttcaagcggcgaaattaaacaagagaaaccgnnnnnnntaagcactaaatttatctacggtaagatttacagaagctcaatataga tagaatacttaatagcattttacggagcttatctttgaagcattgcaggtgcggcagatgaacaggtcagtattaaattctaccatacttgctattcttgcgtaatcttaatggtctcatttaaggttgccatcttgcctttaaaaatcgccaattggcgtaaaatttgtttacgttcaagttttaaactatttaacttttgctgaatttgattggtgtagattttttgtatgctactagtgtaatactatctcttggggaatgtgtttacgttgctagggaatgcgccattcattttgttgattagttgtattcaggcatttattcaatgcattaactgccagctgccagattgtcagcgattatcttcacgcaacaaacgccgccacttttatctcacaggcttcaagcggcgaaattaaacaagagaaaccgaaaaaaataagcactaaatttatctacggtaagatttacagaagctcaatataga Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGAAUACUUAAUAGCAUUUUACGGAGCUUAUCUUUGAAGCAUUGCAGGUGCGGCAGAUGAACAGGUCAGUAUUAAAUUCUACCAUACUUGCUAUUCUUGCGUAAUCUUAAUGGUCUCAUUUAAGGUUGCCAUCUUGCCUUUAAAAAUCGCCAAUUGGCGUAAAAUUUGUUUACGUUCAAGUUUUAAACUAUUUAACUUUUGCUGAAUUUGAUUGGUGUAGAUUUUUUGUAUGCUACUAGUGUAAUACUAUCUCUUGGGGAAUGUGUUUACGUUGCUAGGGAAUGCGCCAUUCAUUUUGUUGAUUAGUUGUAUUCAGGCAUUUAUUCAAUGCAUUAACUGCCAGCUGCCAGAUUGUCAGCGAUUAUCUUCACGCAACAAACGCCGCCACUUUUAUCUCACAGGCUUCAAGCGGCGAAAUUAAACAAGAGAAACCGAAAAAAAUAAGCACUAAAUUUAUCUACGGUAAGAUUUACAGAAGCUCAAUAUAGA ((((((.((....)).))))))..((((((...(((((((((..(((((((.((.(((....................))).)).)))))))......((.((((((((((((....)).))))))))))))...)).)))))))...((((....))))(((((((((........))))))))).......(((.((((((((....((((((((((..(((((((.(((((.....)))))......(((((((...(((........(((((....((((((((...(((......)))...).))))))).((((.(((..........))).)))).((((.(.....).))))............)))))...((((((.......................))))))..)))...)))))))......)))))))..)))))))..)))....)))))))).)))...))))))........ (-110.55) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UCUAUAUUGAGCUUCUGUAAAUCUUACCGUAGAUAAAUUUAGUGCUUAUUUUUUUCGGUUUCUCUUGUUUAAUUUCGCCGCUUGAAGCCUGUGAGAUAAAAGUGGCGGCGUUUGUUGCGUGAAGAUAAUCGCUGACAAUCUGGCAGCUGGCAGUUAAUGCAUUGAAUAAAUGCCUGAAUACAACUAAUCAACAAAAUGAAUGGCGCAUUCCCUAGCAACGUAAACACAUUCCCCAAGAGAUAGUAUUACACUAGUAGCAUACAAAAAAUCUACACCAAUCAAAUUCAGCAAAAGUUAAAUAGUUUAAAACUUGAACGUAAACAAAUUUUACGCCAAUUGGCGAUUUUUAAAGGCAAGAUGGCAACCUUAAAUGAGACCAUUAAGAUUACGCAAGAAUAGCAAGUAUGGUAGAAUUUAAUACUGACCUGUUCAUCUGCCGCACCUGCAAUGCUUCAAAGAUAAGCUCCGUAAAAUGCUAUUAAGUAUUCUA ....(((.((((((......(((((...((((((.......(((((..............(((((((....(((((((.((.....))..)))))))....(((..((((((((((..............((((.(.....).))))..(((.....)))....))))))))))....(((..(((.(((......))).))).((........))...)))..)))......)))))))...............))))).......)))))).............((((...................((((..(((((...........((((.(((.(..........).)))..))))...(((((.((....))))))).)))))))))....(((.((.(((((((....((((......))))..))))))).)).)))..))))...))))))))))).))).((((((....))))))... (-100.69) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 424 406 368 223 188 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 373 416 381 206 177 aact:AA01429|5end_conserved_hypothetical_protein_959581..959811_POSITIVE 356 388 349 111 54 aact:AA01551|5end_hypothetical_protein_1038190..1038420_POSITIVE 353 389 344 109 56 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 351 415 371 212 153 aact:AA02030|5end_threonyl-tRNA_synthetase_1382171..1382401_POSITIVE 351 66 23 112 74 aact:AA01527|5end_hypothetical_protein_1025704..1025934_POSITIVE 348 378 331 170 129 aact:AA00993|5end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694488..694718_POSITIVE 346 67 22 190 141 aact:AA02067|5end_conserved_hypothetical_protein;_possible_membrane_protein_1405233..1405463_POSITIVE 346 67 22 72 23 aact:AA02066|5end_hypothetical_protein_1405115..1405345_POSITIVE 342 388 343 219 178 aact:AA02939|5end_phosphoserine_phosphatase_2056881..2057111_POSITIVE 342 388 342 149 82 aact:AA01684|5end_ABC_transporter,_ATP-binding_protein;_possible_ATP-binding_component_of_sulfate_permease_A_protein_1136302..1136532_POSITIVE 341 413 371 164 113 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 340 362 321 197 156 aact:AA02017|5end_conserved_hypothetical_protein_1371656..1371886_POSITIVE 339 413 378 175 137 aact:AA00386|5end_aspartate--ammonia_ligase_(asparagine_synthetase_A)_261150..261380_POSITIVE 336 389 344 91 28 aact:AA01767|5end_conserved_hypothetical_protein_1192528..1192758_POSITIVE 335 54 15 169 125 aact:AA02369|5end_conserved_hypothetical_protein_1624248..1624478_POSITIVE 335 384 344 50 11 aact:AA01811|5end_electron_transport_complex_protein_1220336..1220566_POSITIVE 334 291 258 62 20 aact:AA00885|5end_ribosomal_large_subunit_pseudouridine_synthase_c_616307..616537_POSITIVE 333 415 372 175 126 aact:AA00735|5end_citrate_lyase,_beta_chain_502674..502904_POSITIVE 328 398 357 79 41 aact:AA00603|5end_undecaprenyl_pyrophosphate_synthetase_(UPPS)_410283..410513_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 356 388 349 133 76 aact:AA01550|3end_hypothetical_protein_1038292..1038442_POSITIVE 346 67 22 94 45 aact:AA02063|3end_23S_rRNA_psedouridine_synthase,_Rlu_family_protein_1405217..1405367_POSITIVE 344 290 255 145 102 aact:AA01428|3end_acetolactate_synthase_959625..959775_POSITIVE 341 413 371 59 8 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 339 413 378 113 75 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 336 389 344 119 56 aact:AA01765|3end_Mu-like_prophage_FluMu_transposase_A_1192636..1192786_POSITIVE 335 54 15 127 83 aact:AA02368|3end_conserved_hypothetical_protein_1624286..1624436_POSITIVE 335 384 344 40 1 aact:AA01810|3end_electron_transport_complex_protein_1220406..1220556_POSITIVE 328 416 371 139 97 aact:AA02602|3end_conserved_hypothetical_protein_1814302..1814452_POSITIVE 327 377 331 86 40 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 324 385 341 79 29 aact:AA01053|3end_conserved_hypothetical_protein_733747..733897_POSITIVE 322 374 331 46 6 aact:AA00374|3end_short-chain_fatty_acids_transporter_253101..253251_POSITIVE 320 418 373 142 93 aact:AA00988|3end_conserved_hypothetical_protein_692264..692414_POSITIVE 319 416 372 99 67 aact:AA01336|3end_hypothetical_protein_904315..904465_POSITIVE 317 415 371 117 63 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 316 388 341 148 98 aact:AA01254|3end_conserved_hypothetical_protein_(possible_proline_dipeptidase)_852702..852852_POSITIVE 314 377 331 75 26 aact:AA00359|3end_50S_ribosomal_protein_L29_244500..244650_POSITIVE 313 387 341 143 94 aact:AA00286|3end_glutamate_5-kinase_(gamma-glutamyl_kinase)_200260..200410_POSITIVE 312 376 331 141 97 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 308 390 341 56 15 aact:AA00992|3end_ribonuclease_BN_694568..694718_POSITIVE