Origin IGS: taagaatttgtgtttacgtaatcagaaacgatcaagtgtttttatcgctttttttaaaaaaagaagcgcagatttttttacaataaatgagattgattatcagttatggaaggggtatgatctgccccctctttctactatttatttaaacagatcacatttaaaaaggaaaagcaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttgcttttcctttttaaatgtgatctgtttaaataaatagtagaaagagggggcagatcataccccttccataactgataatcaatctcatttattgtaaaaaaatctgcgcttctttttttaaaaaaagcgataaaaacacttgatcgtttctgattacgtaaacacaaattctta Mask Tandem Repeat Region ================================================ taagaatttgtgtttacgtaatcagaaacgatcaagtgtttttatcgctttttttaaaaaaagaagcgcagatttttttacaataaatgagattgattatcagttatggaaggggtatgatctgccccctctttctactatttatttaaacagatcacatttaaaaaggaaaagcaa Find is-nt database================================================ Query_seq: AA01123:AA01124|AA01123:AA01124:possible hemolysin; membrane protein:biopolymer transport protein:->->:771718..771894 177 taagaatttgtgtttacgtaatcagaaacgatcaagtgtttttatcgctttttttaaaaaaagaagcgcagatttttttacaataaatgagattgattatcagttatggaaggggtatgatctgccccctctttctactatttatttaaacagatcacatttaaaaaggaaaagcaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01123:AA01124|AA01123:AA01124:possible hemolysin; membrane protein:biopolymer transport protein:->->:771718..771894 177 taagaatttgtgtttacgtaatcagaaacgatcaagtgtttttatcgctttttttaaaaaaagaagcgcagatttttttacaataaatgagattgattatcagttatggaaggggtatgatctgccccctctttctactatttatttaaacagatcacatttaaaaaggaaaagcaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01123:AA01124|AA01123:AA01124:possible hemolysin; membrane protein:biopolymer transport protein:->->:771718..771894 177 taagaatttgtgtttacgtaatcagaaacgatcaagtgtttttatcgctttttttaaaaaaagaagcgcagatttttttacaataaatgagattgattatcagttatggaaggggtatgatctgccccctctttctactatttatttaaacagatcacatttaaaaaggaaaagcaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taagaatttgtgtttacgtaatcagaaacgatcaagtgtttttatcgctttttttaaaaaaagaagcgcagatttttttacaataaatgagattgattatcagttatggaaggggtatgatctgccccctctttctactatttatttaaacagatcacatttaaaaaggaaaagcaa Predict ORF larger than 30AA ================================================ Protein_Len: 56 Strand: + Start: 8 End: 175 ....... M C L R N Q K R S S V F I A F F K K R S A D F F T I N E I D Y Q L W K G Y D L P P L S T I Y L N R S H L K R K S .. taagaatttgtgtttacgtaatcagaaacgatcaagtgtttttatcgctttttttaaaaaaagaagcgcagatttttttacaataaatgagattgattatcagttatggaaggggtatgatctgccccctctttctactatttatttaaacagatcacatttaaaaaggaaaagcaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 84 Start: 92 End: 121 ...........................................................................................ATTGATTATCAGTTATGGAAGGGGTATGAT........................................................ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taagaatttgtgtttacgtaatcagaaacgatcaagtgtttttatcgctttttttaaaaaaagaagcgcagatttttttacaataaatgagattgattatcagttatggaaggggtatgatctgccccctctttctactatttatttaaacagatcacatttaaaaaggaaaagcaa Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 72 HP_score: -2.6 Tail_Score: -5.44119 Start: 42 End: 72 Full_Region: AACGATCAAGTGTTT TTATCGCTTTTTTT AAA AAAAGAAGCGCAGA TTTTTTTACAATAAA .........................................TTATCGCTTTTTTTAAAAAAAGAAGCGCAGA......................................................................................................... TransTerm Strand: + Conf: 68 HP_score: -5.5 Tail_Score: -4.12941 Start: 109 End: 131 Full_Region: TGATTATCAGTTATG GAAGGGGTA TGATC TGCCCCCTC TTTCTACTATTTATT ............................................................................................................GAAGGGGTATGATCTGCCCCCTC.............................................. Find igs database================================================ Query_seq: AA01123:AA01124|AA01123:AA01124:possible hemolysin; membrane protein:biopolymer transport protein:->->:771718..771894 177 taagaatttgtgtttacgtaatcagaaacgatcaagtgtttttatcgctttttttaaaaaaagaagcgcagatttttttacaataaatgagattgattatcagttatggaaggggtatgatctgccccctctttctactatttatttaaacagatcacatttaaaaaggaaaagcaa Intra-Species Hit: Count: 1 Min: 1 Max: 177 Len: 177 Subject: aact_AA01123_AA01124|possible hemolysin; membrane protein:biopolymer transport protein|POSITIVE:POSITIVE|[771718,771894]|177 HSP 1 e-value: 7.0E-49 bit: 194.0 Len: 98 Query Start:80 Query End:177 Subject Strand: POSITIVE Subject Start: 80 Subject End: 177 ...............................................................................acaataaatgagattgattatcagttatggaaggggtatgatctgccccctctttctactatttatttaaacagatcacatttaaaaaggaaaagcaa ...............................................................................acaataaatgagattgattatcagttatggaaggggtatgatctgccccctctttctactatttatttaaacagatcacatttaaaaaggaaaagcaa HSP 2 e-value: 5.0E-19 bit: 95.6 Len: 48 Query Start:1 Query End:48 Subject Strand: POSITIVE Subject Start: 1 Subject End: 48 taagaatttgtgtttacgtaatcagaaacgatcaagtgtttttatcgc................................................................................................................................. taagaatttgtgtttacgtaatcagaaacgatcaagtgtttttatcgc................................................................................................................................. Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAGAAUUUGUGUUUACGUAAUCAGAAACGAUCAAGUGUUUUUAUCGCUUUUUUUAAAAAAAGAAGCGCAGAUUUUUUUACAAUAAAUGAGAUUGAUUAUCAGUUAUGGAAGGGGUAUGAUCUGCCCCCUCUUUCUACUAUUUAUUUAAACAGAUCACAUUUAAAAAGGAAAAGCAA ................(((........)))......(((((((..(((((((((.....)))))))))..(((((.((((.((((((((.(((((.....)))))..(((.((((((.....)))))).)))......)))))))))))).))))).............))))))). (-35.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGCUUUUCCUUUUUAAAUGUGAUCUGUUUAAAUAAAUAGUAGAAAGAGGGGGCAGAUCAUACCCCUUCCAUAACUGAUAAUCAAUCUCAUUUAUUGUAAAAAAAUCUGCGCUUCUUUUUUUAAAAAAAGCGAUAAAAACACUUGAUCGUUUCUGAUUACGUAAACACAAAUUCUUA .................((((((((...............((((((((((((((((((..........(.((((.(((.........))).)))).).......)))))).))))))))))))....(((((((.((.....)).)))))))..))))))))............... (-30.33) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 302 129 106 103 83 aact:AA01269|5end_hypothetical_protein_863172..863402_POSITIVE 295 129 97 170 145 aact:AA02343|5end_glutamine_synthetase_1600173..1600403_POSITIVE 290 155 112 179 123 aact:AA03009|5end_hypothetical_protein_2101001..2101231_POSITIVE 282 130 86 178 139 aact:AA00979|5end_type_B_carboxylesterase;_p-nitrobenzyl_esterase_684645..684875_POSITIVE 267 134 91 110 66 aact:AA01649|5end_maltose_ABC_transporter,_permease_protein_1117316..1117546_POSITIVE 257 50 16 60 28 aact:AA02893|5end_oligopeptide_transport_system_permease_protein,_N-terminal_2027201..2027431_POSITIVE 248 130 102 36 5 aact:AA02657|5end_conserved_hypothetical_protein_1852012..1852242_POSITIVE 237 143 109 208 170 aact:AA01379|5end_glycerophosphoryl_diester_phosphodiesterase_(protein_D)_933029..933259_POSITIVE 236 48 5 60 15 aact:AA01772|5end_shikimate_5-dehydrogenase_protein_1194050..1194280_POSITIVE 236 49 5 61 10 aact:AA00669|5end_cytolethal_distending_toxin_protein_A_459380..459610_POSITIVE 234 135 103 197 164 aact:AA02949|5end_hypothetical_protein_2061944..2062174_POSITIVE 234 136 97 210 167 aact:AA01848|5end_argininosuccinate_synthetase_1242278..1242508_POSITIVE 234 129 86 181 136 aact:AA01446|5end_conserved_hypothetical_protein_970151..970381_POSITIVE 233 69 21 200 147 aact:AA01424|5end_threonine_deaminase_(dehydratase)_957740..957970_POSITIVE 232 50 6 64 11 aact:AA01928|5end_ATP-dependent_RNA_helicase_1301986..1302216_POSITIVE 230 55 22 165 130 aact:AA00625|5end_hypothetical_protein_430565..430795_POSITIVE 230 55 22 57 22 aact:AA00623|5end_hypothetical_protein_430457..430687_POSITIVE 229 49 12 142 106 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 229 135 100 80 43 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 227 129 84 126 60 aact:AA00536|5end_5'-nucleotidase_precursor_366151..366381_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 302 129 106 68 48 aact:AA01268|3end_transposase_863217..863367_POSITIVE 295 129 97 68 43 aact:AA02342|3end_ISRSO11/IS150_related_transposase,_orfB_1600151..1600301_POSITIVE 260 130 88 38 1 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 252 147 101 97 44 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE 252 147 101 97 44 aact:AA01261|3end_conserved_hypothetical_protein_856723..856873_POSITIVE 234 135 103 47 14 aact:AA02947|3end_riboflavin_biosynthesis_protein_2061874..2062024_POSITIVE 234 129 86 57 12 aact:AA01445|3end_hypothetical_protein_970107..970257_POSITIVE 233 69 21 60 7 aact:AA01423|3end_cell_division_protein_X,_ABC_system_permease_957680..957830_POSITIVE 233 130 84 43 6 aact:AA01402|3end_beta_1-4_glucosyltransferase_948382..948532_POSITIVE 231 134 93 77 26 aact:AA01378|3end_glycerol-3-phosphatase_transporter_932872..933022_POSITIVE 229 49 12 37 1 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 225 69 23 66 9 aact:AA00668|3end_cytolethal_distending_toxin_protein_B_459442..459592_POSITIVE 224 129 107 68 48 aact:AA01077|3end_transposase_746870..747020_POSITIVE 221 54 22 151 117 aact:AA00623|3end_hypothetical_protein_430632..430782_POSITIVE 220 135 102 97 59 aact:AA00805|3end_fumarate_reductase_iron-sulfur_protein_552430..552580_POSITIVE 215 133 102 58 15 aact:AA01756|3end_cobalt_membrane_transport_protein_1187217..1187367_POSITIVE 215 131 92 147 112 aact:AA00694|3end_dCTP_deaminase_(deoxycytidine_triphosphate_deaminas)_475100..475250_POSITIVE 213 130 86 50 15 aact:AA02114|3end_exodeoxyribonuclease_I_1444570..1444720_POSITIVE 213 126 81 149 104 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 213 126 81 114 74 aact:AA00182|3end_biotin_carboxylase_(subunit_of_acetyl-CoA_carboxylase)_130235..130385_POSITIVE