Origin IGS: taatccctttctgaaaacaaaaccccatacgcggtgtatgaggttctttttatagccagacgttgtctggctataacaaaagtgcggtcattctaaaaaacgttttttaaaatgaccgcacttttttttgttttcatgatggttgagggtgatggcta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tagccatcaccctcaaccatcatgaaaacaaaaaaaagtgcggtcattttaaaaaacgttttttagaatgaccgcacttttgttatagccagacaacgtctggctataaaaagaacctcatacaccgcgtatggggttttgttttcagaaagggatta Mask Tandem Repeat Region ================================================ taatccctttctgaaaacaaaaccccatacgcggtgtatgaggttctttttatagccagacgttgtctggctataacaaaagtgcggtcattctaaaaaacgttttttaaaatgaccgcacttttttttgttttcatgatggttgagggtgatggcta Find is-nt database================================================ Query_seq: AA01116:AA01117|AA01116:AA01117:hypothetical protein:signal recognition particle protein 54 (GTP-binding export factor):->->:767901..768058 158 taatccctttctgaaaacaaaaccccatacgcggtgtatgaggttctttttatagccagacgttgtctggctataacaaaagtgcggtcattctaaaaaacgttttttaaaatgaccgcacttttttttgttttcatgatggttgagggtgatggcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01116:AA01117|AA01116:AA01117:hypothetical protein:signal recognition particle protein 54 (GTP-binding export factor):->->:767901..768058 158 taatccctttctgaaaacaaaaccccatacgcggtgtatgaggttctttttatagccagacgttgtctggctataacaaaagtgcggtcattctaaaaaacgttttttaaaatgaccgcacttttttttgttttcatgatggttgagggtgatggcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01116:AA01117|AA01116:AA01117:hypothetical protein:signal recognition particle protein 54 (GTP-binding export factor):->->:767901..768058 158 taatccctttctgaaaacaaaaccccatacgcggtgtatgaggttctttttatagccagacgttgtctggctataacaaaagtgcggtcattctaaaaaacgttttttaaaatgaccgcacttttttttgttttcatgatggttgagggtgatggcta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatccctttctgaaaacaaaaccccatacgcggtgtatgaggttctttttatagccagacgttgtctggctataacaaaagtgcggtcattctaaaaaacgttttttaaaatgaccgcacttttttttgttttcatgatggttgagggtgatggcta Predict ORF larger than 30AA ================================================ taatccctttctgaaaacaaaaccccatacgcggtgtatgaggttctttttatagccagacgttgtctggctataacaaaagtgcggtcattctaaaaaacgttttttaaaatgaccgcacttttttttgttttcatgatggttgagggtgatggcta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatccctttctgaaaacaaaaccccatacgcggtgtatgaggttctttttatagccagacgttgtctggctataacaaaagtgcggtcattctaaaaaacgttttttaaaatgaccgcacttttttttgttttcatgatggttgagggtgatggcta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 93 HP_score: -7.6 Tail_Score: -5.07652 Start: 23 End: 43 Full_Region: tggctataaaaagaa cctcatac accgc gtatgggg ttttgttttcagaaa ......................cctcatacaccgcgtatgggg................................................................................................................... TransTerm Strand: - Conf: 100 HP_score: -20.5 Tail_Score: -3.28712 Start: 77 End: 126 Full_Region: catcatgaaaacaaa aaaaagtgcggtcattttaaaaa acgt tttttagaatgaccgcacttttg ttatagccagacaac ............................................................................aaaaagtgcggtcattttaaaaaacgttttttagaatgaccgcacttttg................................ TransTerm Strand: + Conf: 100 HP_score: -18.2 Tail_Score: -6.34616 Start: 82 End: 121 Full_Region: CTGGCTATAACAAAA GTGCGGTCATTCTAAAAA ACGT TTTTTAAAATGACCGCAC TTTTTTTTGTTTTCA .................................................................................GTGCGGTCATTCTAAAAAACGTTTTTTAAAATGACCGCAC..................................... TransTerm Strand: - Conf: 100 HP_score: -20.4 Tail_Score: -4.77165 Start: 82 End: 121 Full_Region: tgaaaacaaaaaaaa gtgcggtcattttaaaaa acgt tttttagaatgaccgcac ttttgttatagccag .................................................................................gtgcggtcattttaaaaaacgttttttagaatgaccgcac..................................... Find igs database================================================ Query_seq: AA01116:AA01117|AA01116:AA01117:hypothetical protein:signal recognition particle protein 54 (GTP-binding export factor):->->:767901..768058 158 taatccctttctgaaaacaaaaccccatacgcggtgtatgaggttctttttatagccagacgttgtctggctataacaaaagtgcggtcattctaaaaaacgttttttaaaatgaccgcacttttttttgttttcatgatggttgagggtgatggcta Intra-Species Hit: Count: 1 Min: 1 Max: 158 Len: 158 Subject: aact_AA01116_AA01117|hypothetical protein:signal recognition particle protein 54 (GTP-binding export factor)|POSITIVE:POSITIVE|[767901,768058]|158 HSP 1 e-value: 2.0E-70 bit: 266.0 Len: 158 Query Start:1 Query End:158 Subject Strand: POSITIVE Subject Start: 1 Subject End: 158 taatccctttctgaaaacaaaaccccatacgcggtgtatgaggttctttttatagccagacgttgtctggctataacaaaagtgcggtcattctaaaaaacgttttttaaaatgaccgcacnnnnnnnngttttcatgatggttgagggtgatggcta taatccctttctgaaaacaaaaccccatacgcggtgtatgaggttctttttatagccagacgttgtctggctataacaaaagtgcggtcattctaaaaaacgttttttaaaatgaccgcacttttttttgttttcatgatggttgagggtgatggcta HSP 2 e-value: 6.0E-12 bit: 71.9 Len: 44 Query Start:78 Query End:121 Subject Strand: NEGATIVE Subject Start: 82 Subject End: 125 .............................................................................aaaagtgcggtcattctaaaaaacgttttttaaaatgaccgcac..................................... .............................................................................aaaagtgcggtcattttaaaaaacgttttttagaatgaccgcac..................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCCCUUUCUGAAAACAAAACCCCAUACGCGGUGUAUGAGGUUCUUUUUAUAGCCAGACGUUGUCUGGCUAUAACAAAAGUGCGGUCAUUCUAAAAAACGUUUUUUAAAAUGACCGCACUUUUUUUUGUUUUCAUGAUGGUUGAGGGUGAUGGCUA ....((((((((((((((((((((.(((((.....))))).))))....((((((((((((...)))))))))))).(((((((((((((((.((((((....)))))).)))))))))))))))..))))))))).)).....)))))......... (-62.50) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGCCAUCACCCUCAACCAUCAUGAAAACAAAAAAAAGUGCGGUCAUUUUAAAAAACGUUUUUUAGAAUGACCGCACUUUUGUUAUAGCCAGACAACGUCUGGCUAUAAAAAGAACCUCAUACACCGCGUAUGGGGUUUUGUUUUCAGAAAGGGAUUA .........((((......((.((((((((...((((((((((((((((((((((....)))))))))))))))))))))).((((((((((((...))))))))))))...(((((((((((.....))))))))))))))))))))).)))).... (-71.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 577 125 81 221 177 aact:AA02778|5end_hypothetical_protein_1942585..1942815_POSITIVE 561 128 81 155 108 aact:AA01648|5end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1115170..1115400_POSITIVE 561 128 81 54 7 aact:AA01646|5end_hypothetical_protein_1115069..1115299_POSITIVE 530 124 81 159 116 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 507 126 81 171 126 aact:AA00721|5end_probable_beta-N-acetylhexosaminidase_(lacto-N-biosidase);_dispersin_B_494739..494969_POSITIVE 507 126 81 59 14 aact:AA00719|5end_hypothetical_protein_494627..494857_POSITIVE 491 127 81 166 120 aact:AA01967|5end_hypothetical_protein_1331511..1331741_POSITIVE 481 126 81 152 107 aact:AA02888|5end_ssrA-binding_protein,_small_protein_B_2024628..2024858_POSITIVE 477 124 81 151 108 aact:AA00386|5end_aspartate--ammonia_ligase_(asparagine_synthetase_A)_261150..261380_POSITIVE 472 130 81 200 154 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 460 124 81 158 115 aact:AA02700|5end_succinyl-CoA_synthase,_alpha_subunit_1883256..1883486_POSITIVE 421 126 81 178 136 aact:AA00212|5end_ribokinase_146056..146286_POSITIVE 421 126 81 77 35 aact:AA00211|5end_hypothetical_protein_145955..146185_POSITIVE 420 129 81 159 116 aact:AA02113|5end_conserved_hypothetical_protein_1442183..1442413_POSITIVE 416 125 81 123 81 aact:AA01393|5end_ABC_transporter,_ATP-binding_protein_940369..940599_POSITIVE 399 73 31 231 189 aact:AA01226|5end_IS200-like_transposase_ISAa1_838066..838296_POSITIVE 399 129 81 127 82 aact:AA00792|5end_conserved_hypothetical_protein_543142..543372_POSITIVE 380 126 81 124 73 aact:AA02276|5end_pseudouridine_synthase_Rlu_family_1549932..1550162_POSITIVE 379 130 81 184 137 aact:AA00654|5end_conserved_hypothetical_protein_447772..448002_POSITIVE 379 130 81 94 47 aact:AA00653|5end_hypothetical_protein_447682..447912_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 577 125 81 83 39 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 561 128 81 89 42 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 507 126 81 75 30 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE 491 127 81 89 43 aact:AA01966|3end_2-amino-4-hydroxy-6-hydroxymethyldihydropteridine_pyrophosphokinase_1331514..1331664_POSITIVE 485 124 83 42 1 aact:AA00764|3end_hemoglobin_binding_protein_A_527347..527497_POSITIVE 481 126 81 78 33 aact:AA02886|3end_OBG-related_GTP-binding_protein_2024634..2024784_POSITIVE 477 124 81 89 46 aact:AA00384|3end_conserved_hypothetical_protein_(possible_nitrogen_fixation_protein)_261168..261318_POSITIVE 472 130 81 87 41 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 421 126 81 74 32 aact:AA00209|3end_D-ribose_ABC_transporter,_periplasmic-binding_protein_146032..146182_POSITIVE 420 129 81 80 37 aact:AA02112|3end_cell_division_protein_1442184..1442334_POSITIVE 416 125 81 76 34 aact:AA01391|3end_glycerol-3-phosphate_acyltransferase_940402..940552_POSITIVE 408 120 75 71 26 aact:AA02562|3end_adenine_specific_methylase_1785146..1785296_POSITIVE 394 130 81 63 19 aact:AA00010|3end_transposase_orfB_fragment_6297..6447_POSITIVE 391 126 81 77 32 aact:AA00629|3end_inosine_5'_monophosphate_dehydrogenase_(IMP_dehydrogenase)_433469..433619_POSITIVE 387 127 81 89 48 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 385 85 49 40 4 aact:AA01243|3end_50S_ribosomal_protein_L17_847440..847590_POSITIVE 384 120 75 74 32 aact:AA00218|3end_argininosuccinate_lyase_152211..152361_POSITIVE 380 126 81 67 16 aact:AA02275|3end_conserved_hypothetical_protein_1549955..1550105_POSITIVE 378 123 81 60 18 aact:AA00355|3end_DNA-directed_RNA_polymerase_beta_chain_(transcriptase_beta_chain)_238911..239061_POSITIVE 367 128 81 54 11 aact:AA02992|3end_RNA_methyltransferase_2091740..2091890_POSITIVE