Origin IGS: taatcggttctagcgaggctaaattttccatcaaagtgcagtgaaatgaagcgtccatagcccctattatttaaggaattttaatgagtctattcatgaaaatcatcaaatgactcatgattttggcgcagcgggctaggtgtttttaaactatcactctgtttggggcagcacacctgatgctgcaagtcttgctaaaatgtaggtttcaaaacatgttgacggcacgaaatttaacaatccggaagtactccctgtgcgtactgccacaaaagaagagcaaacctcattgctcggttggacatggaaaatgatcaacctttcccgcgggtaaattcgacctagacaatcactc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gagtgattgtctaggtcgaatttacccgcgggaaaggttgatcattttccatgtccaaccgagcaatgaggtttgctcttcttttgtggcagtacgcacagggagtacttccggattgttaaatttcgtgccgtcaacatgttttgaaacctacattttagcaagacttgcagcatcaggtgtgctgccccaaacagagtgatagtttaaaaacacctagcccgctgcgccaaaatcatgagtcatttgatgattttcatgaatagactcattaaaattccttaaataataggggctatggacgcttcatttcactgcactttgatggaaaatttagcctcgctagaaccgatta Mask Tandem Repeat Region ================================================ taatcggttctagcgaggctaaattttccatcaaagtgcagtgaaatgaagcgtccatagcccctattatttaaggaattttaatgagtctattcatgaaaatcatcaaatgactcatgattttggcgcagcgggctaggtgtttttaaactatcactctgtttggggcagcacacctgatgctgcaagtcttgctaaaatgtaggtttcaaaacatgttgacggcacgaaatttaacaatccggaagtactccctgtgcgtactgccacaaaagaagagcaaacctcattgctcggttggacatggaaaatgatcaacctttcccgcgggtaaattcgacctagacaatcactc Find is-nt database================================================ Query_seq: AA01115:AA01116|AA01115:AA01116:carboxylesterase:hypothetical protein:->->:767414..767768 355 taatcggttctagcgaggctaaattttccatcaaagtgcagtgaaatgaagcgtccatagcccctattatttaaggaattttaatgagtctattcatgaaaatcatcaaatgactcatgattttggcgcagcgggctaggtgtttttaaactatcactctgtttggggcagcacacctgatgctgcaagtcttgctaaaatgtaggtttcaaaacatgttgacggcacgaaatttaacaatccggaagtactccctgtgcgtactgccacaaaagaagagcaaacctcattgctcggttggacatggaaaatgatcaacctttcccgcgggtaaattcgacctagacaatcactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01115:AA01116|AA01115:AA01116:carboxylesterase:hypothetical protein:->->:767414..767768 355 taatcggttctagcgaggctaaattttccatcaaagtgcagtgaaatgaagcgtccatagcccctattatttaaggaattttaatgagtctattcatgaaaatcatcaaatgactcatgattttggcgcagcgggctaggtgtttttaaactatcactctgtttggggcagcacacctgatgctgcaagtcttgctaaaatgtaggtttcaaaacatgttgacggcacgaaatttaacaatccggaagtactccctgtgcgtactgccacaaaagaagagcaaacctcattgctcggttggacatggaaaatgatcaacctttcccgcgggtaaattcgacctagacaatcactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01115:AA01116|AA01115:AA01116:carboxylesterase:hypothetical protein:->->:767414..767768 355 taatcggttctagcgaggctaaattttccatcaaagtgcagtgaaatgaagcgtccatagcccctattatttaaggaattttaatgagtctattcatgaaaatcatcaaatgactcatgattttggcgcagcgggctaggtgtttttaaactatcactctgtttggggcagcacacctgatgctgcaagtcttgctaaaatgtaggtttcaaaacatgttgacggcacgaaatttaacaatccggaagtactccctgtgcgtactgccacaaaagaagagcaaacctcattgctcggttggacatggaaaatgatcaacctttcccgcgggtaaattcgacctagacaatcactc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatcggttctagcgaggctaaattttccatcaaagtgcagtgaaatgaagcgtccatagcccctattatttaaggaattttaatgagtctattcatgaaaatcatcaaatgactcatgattttggcgcagcgggctaggtgtttttaaactatcactctgtttggggcagcacacctgatgctgcaagtcttgctaaaatgtaggtttcaaaacatgttgacggcacgaaatttaacaatccggaagtactccctgtgcgtactgccacaaaagaagagcaaacctcattgctcggttggacatggaaaatgatcaacctttcccgcgggtaaattcgacctagacaatcactc Predict ORF larger than 30AA ================================================ Protein_Len: 53 Strand: + Start: 153 End: 311 ........................................................................................................................................................ M T L F G A A H L M L Q V L L K C R F Q N M L T A R N L T I R K Y S L C V L P Q K K S K P H C S V G H G K ............................................ Protein_Len: 60 Strand: - Start: 157 End: 336 ............................................................................................................................................................ E T Q P L V G S A A L R A L I Y T E F C T S P V F N L L G S T S G T R V A V F S S C V E N S P Q V H F I I L R E R P Y M ................... Protein_Len: 32 Strand: - Start: 93 End: 188 ............................................................................................ E H F D D F S E H N Q R L P S P T K L S D S Q K P C C V Q H Q M ....................................................................................................................................................................... taatcggttctagcgaggctaaattttccatcaaagtgcagtgaaatgaagcgtccatagcccctattatttaaggaattttaatgagtctattcatgaaaatcatcaaatgactcatgattttggcgcagcgggctaggtgtttttaaactatcactctgtttggggcagcacacctgatgctgcaagtcttgctaaaatgtaggtttcaaaacatgttgacggcacgaaatttaacaatccggaagtactccctgtgcgtactgccacaaaagaagagcaaacctcattgctcggttggacatggaaaatgatcaacctttcccgcgggtaaattcgacctagacaatcactc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatcggttctagcgaggctaaattttccatcaaagtgcagtgaaatgaagcgtccatagcccctattatttaaggaattttaatgagtctattcatgaaaatcatcaaatgactcatgattttggcgcagcgggctaggtgtttttaaactatcactctgtttggggcagcacacctgatgctgcaagtcttgctaaaatgtaggtttcaaaacatgttgacggcacgaaatttaacaatccggaagtactccctgtgcgtactgccacaaaagaagagcaaacctcattgctcggttggacatggaaaatgatcaacctttcccgcgggtaaattcgacctagacaatcactc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 70 HP_score: -5.6 Tail_Score: -4.31397 Start: 278 End: 295 Full_Region: tttccatgtccaacc gagcaa tgaggt ttgctc ttcttttgtggcagt .....................................................................................................................................................................................................................................................................................gagcaatgaggtttgctc............................................................ TransTerm Strand: + Conf: 39 HP_score: -2.4 Tail_Score: -2.53263 Start: 280 End: 293 Full_Region: TGCCACAAAAGAAGA GCAA ACCTCA TTGC TCGGTTGGACATGGA .......................................................................................................................................................................................................................................................................................GCAAACCTCATTGC.............................................................. TransTerm Strand: + Conf: 55 HP_score: -5.0 Tail_Score: -3.53811 Start: 296 End: 320 Full_Region: CAAACCTCATTGCTC GGTTGGACAT GGAAA ATGATCAACC TTTCCCGCGGGTAAA .......................................................................................................................................................................................................................................................................................................GGTTGGACATGGAAAATGATCAACC................................... Find igs database================================================ Query_seq: AA01115:AA01116|AA01115:AA01116:carboxylesterase:hypothetical protein:->->:767414..767768 355 taatcggttctagcgaggctaaattttccatcaaagtgcagtgaaatgaagcgtccatagcccctattatttaaggaattttaatgagtctattcatgaaaatcatcaaatgactcatgattttggcgcagcgggctaggtgtttttaaactatcactctgtttggggcagcacacctgatgctgcaagtcttgctaaaatgtaggtttcaaaacatgttgacggcacgaaatttaacaatccggaagtactccctgtgcgtactgccacaaaagaagagcaaacctcattgctcggttggacatggaaaatgatcaacctttcccgcgggtaaattcgacctagacaatcactc Intra-Species Hit: Count: 1 Min: 1 Max: 355 Len: 355 Subject: aact_AA01115_AA01116|carboxylesterase:hypothetical protein|POSITIVE:POSITIVE|[767414,767768]|355 HSP 1 e-value: 0.0 bit: 704.0 Len: 355 Query Start:1 Query End:355 Subject Strand: POSITIVE Subject Start: 1 Subject End: 355 taatcggttctagcgaggctaaattttccatcaaagtgcagtgaaatgaagcgtccatagcccctattatttaaggaattttaatgagtctattcatgaaaatcatcaaatgactcatgattttggcgcagcgggctaggtgtttttaaactatcactctgtttggggcagcacacctgatgctgcaagtcttgctaaaatgtaggtttcaaaacatgttgacggcacgaaatttaacaatccggaagtactccctgtgcgtactgccacaaaagaagagcaaacctcattgctcggttggacatggaaaatgatcaacctttcccgcgggtaaattcgacctagacaatcactc taatcggttctagcgaggctaaattttccatcaaagtgcagtgaaatgaagcgtccatagcccctattatttaaggaattttaatgagtctattcatgaaaatcatcaaatgactcatgattttggcgcagcgggctaggtgtttttaaactatcactctgtttggggcagcacacctgatgctgcaagtcttgctaaaatgtaggtttcaaaacatgttgacggcacgaaatttaacaatccggaagtactccctgtgcgtactgccacaaaagaagagcaaacctcattgctcggttggacatggaaaatgatcaacctttcccgcgggtaaattcgacctagacaatcactc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUCGGUUCUAGCGAGGCUAAAUUUUCCAUCAAAGUGCAGUGAAAUGAAGCGUCCAUAGCCCCUAUUAUUUAAGGAAUUUUAAUGAGUCUAUUCAUGAAAAUCAUCAAAUGACUCAUGAUUUUGGCGCAGCGGGCUAGGUGUUUUUAAACUAUCACUCUGUUUGGGGCAGCACACCUGAUGCUGCAAGUCUUGCUAAAAUGUAGGUUUCAAAACAUGUUGACGGCACGAAAUUUAACAAUCCGGAAGUACUCCCUGUGCGUACUGCCACAAAAGAAGAGCAAACCUCAUUGCUCGGUUGGACAUGGAAAAUGAUCAACCUUUCCCGCGGGUAAAUUCGACCUAGACAAUCACUC .((((.(((.((((((((((.......(((.((.((((.(((.....((((((.((.((((((((.........((((((...(((((((.....(((.....))).....))))))))))))).....)).))))))))))))))...)))..)))).))..))).(((((((....).)))))).)))))))))).)))...))))........((((...((..((((.((((...........((((.....))))...((((..........((((((......))))))((((((.(((.....))).))))))......)))).)))))))).))..))))....... (-93.44) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAGUGAUUGUCUAGGUCGAAUUUACCCGCGGGAAAGGUUGAUCAUUUUCCAUGUCCAACCGAGCAAUGAGGUUUGCUCUUCUUUUGUGGCAGUACGCACAGGGAGUACUUCCGGAUUGUUAAAUUUCGUGCCGUCAACAUGUUUUGAAACCUACAUUUUAGCAAGACUUGCAGCAUCAGGUGUGCUGCCCCAAACAGAGUGAUAGUUUAAAAACACCUAGCCCGCUGCGCCAAAAUCAUGAGUCAUUUGAUGAUUUUCAUGAAUAGACUCAUUAAAAUUCCUUAAAUAAUAGGGGCUAUGGACGCUUCAUUUCACUGCACUUUGAUGGAAAAUUUAGCCUCGCUAGAACCGAUUA ...(((((((((((..........((....))..(((((((..(((((((((........((((((......))))))....((((.((((((((((...((((....))))(..((((((((....(((...((((......))))....)))..))))))))..)............)))))))))).))))(((((((.((((............((....))((((((.....(((((((..((.(((.....))).))..)))))))......((((((.....))))))...))).)))........)))))))))))))))))))))))))))..)))))..)))))) (-95.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 375 180 131 201 160 aact:AA00161|5end_pilin_B_biogenesis_protein;_fimbrial_transcription_regulation_113384..113614_POSITIVE 338 209 161 221 178 aact:AA02502|5end_adenine_phosphoribosyltransferase_1737582..1737812_POSITIVE 337 168 121 74 24 aact:AA00833|5end_UDP-N-acetylmuramate-alanine_ligase_573711..573941_POSITIVE 337 332 295 163 114 aact:AA00280|5end_adenosylmethionine-8-amino-7-oxononanoate;_7,8-diamino-perlargonic_acid_ATase_196430..196660_POSITIVE 335 198 154 150 99 aact:AA02049|5end_hypothetical_protein_1395526..1395756_POSITIVE 334 142 101 48 5 aact:AA02870|5end_conserved_hypothetical_protein_2014123..2014353_POSITIVE 333 281 241 70 24 aact:AA02532|5end_conserved_hypothetical_protein_1759566..1759796_POSITIVE 331 330 288 222 173 aact:AA02899|5end_oligopeptide_ABC_transporter,_ATP-binding_protein_2030063..2030293_POSITIVE 329 197 152 126 70 aact:AA02676|5end_formate_dehydrogenase_alpha_subunit_1861671..1861901_POSITIVE 328 270 223 207 174 aact:AA01421|5end_phosphoglycolate_phosphatase_955055..955285_POSITIVE 325 329 284 202 162 aact:AA02507|5end_penicillin-insensitive_murein_endopeptidase_A_1740058..1740288_POSITIVE 324 168 124 145 110 aact:AA00525|5end_hypothetical_protein_359254..359484_POSITIVE 314 269 242 156 126 aact:AA00235|5end_hypothetical_protein_168596..168826_POSITIVE 313 229 182 83 43 aact:AA00531|5end_DNA_polymerase_I_362621..362851_POSITIVE 312 210 162 204 148 aact:AA01188|5end_conserved_hypothetical_protein_813912..814142_POSITIVE 311 270 223 109 62 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 311 209 166 75 33 aact:AA00347|5end_50S_ribosomal_protein_L11_232137..232367_POSITIVE 309 177 137 229 184 aact:AA00305|5end_conserved_hypothetical_protein_206741..206971_POSITIVE 308 329 283 103 51 aact:AA01995|5end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355120..1355350_POSITIVE 306 166 123 107 57 aact:AA02358|5end_homoserine_kinase_1617128..1617358_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 408 78 31 85 43 aact:AA02417|3end_GTP-binding_protein_1660978..1661128_POSITIVE 337 168 121 58 8 aact:AA00832|3end_D-alanine-D-alanine_ligase_B_573775..573925_POSITIVE 335 198 154 123 72 aact:AA02048|3end_galactitol_utilization_operon_repressor_1395579..1395729_POSITIVE 329 197 152 74 18 aact:AA02675|3end_formate_dehydrogenase_alpha_subunit_1861699..1861849_POSITIVE 314 269 242 121 91 aact:AA00234|3end_collagen_adhesin_168641..168791_POSITIVE 313 229 182 50 10 aact:AA00530|3end_hypothetical_protein_362668..362818_POSITIVE 306 166 123 91 41 aact:AA02357|3end_bifunctional_protein:_aspartokinase_I;_homoserine_dehydrogenaseI_1617192..1617342_POSITIVE 300 169 121 140 104 aact:AA00407|3end_trk_system_potassium_uptake_protein_272309..272459_POSITIVE 299 339 299 124 92 aact:AA01024|3end_thiamine_biosynthesis_lipoprotein_precursor_717602..717752_POSITIVE 296 297 251 73 29 aact:AA00514|3end_hypothetical_protein_355838..355988_POSITIVE 294 170 124 113 65 aact:AA01189|3end_conserved_hypothetical_protein_(possible_glycosyl_transferase)_815358..815508_POSITIVE 293 145 113 147 120 aact:AA00726|3end_apolipoprotein_N-acyltransferase,_copper_499886..500036_POSITIVE 289 328 283 148 102 aact:AA01994|3end_chorismate_mutase;_prephenate_dehydrogenase_1355251..1355401_POSITIVE 289 175 131 91 37 aact:AA01158|3end_ribosome_associated_heat_shock_protein_15_homolog_792892..793042_POSITIVE 288 142 102 47 7 aact:AA02677|3end_formate_dehydrogenase,_beta_subunit_1865105..1865255_POSITIVE 287 270 223 68 4 aact:AA00316|3end_conserved_hypothetical_protein_215340..215490_POSITIVE 286 327 287 65 28 aact:AA01524|3end_amino_acid_ABC_transporter,_ATP-binding_protein_1024344..1024494_POSITIVE 285 224 181 150 108 aact:AA00396|3end_conserved_hypothetical_protein_265952..266102_POSITIVE 284 55 12 88 55 aact:AA01896|3end_hypothetical_protein_1281163..1281313_POSITIVE 284 142 121 151 133 aact:AA01360|3end_XAA-pro_aminopeptidase_(X-pro_aminopeptidase)_922425..922575_POSITIVE