Origin IGS: taaaaaacggcttgattatggaaaaaattgaacaacaccaaagttttggtggcgagcaacaagtttggacgcattttgcccaaactttacaatgcgagatgaaggtggcggtttat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ataaaccgccaccttcatctcgcattgtaaagtttgggcaaaatgcgtccaaacttgttgctcgccaccaaaactttggtgttgttcaattttttccataatcaagccgtttttta Mask Tandem Repeat Region ================================================ taaaaaacggcttgattatggaaaaaattgaacaacaccaaagttttggtggcgagcaacaagtttggacgcattttgcccaaactttacaatgcgagatgaaggtggcggtttat Find is-nt database================================================ Query_seq: AA01114:AA01115|AA01114:AA01115:alcohol dehydrogenase, class III; glutathione-dependent formaldehyde dehydrogenase:carboxylesterase:->->:766569..766684 116 taaaaaacggcttgattatggaaaaaattgaacaacaccaaagttttggtggcgagcaacaagtttggacgcattttgcccaaactttacaatgcgagatgaaggtggcggtttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01114:AA01115|AA01114:AA01115:alcohol dehydrogenase, class III; glutathione-dependent formaldehyde dehydrogenase:carboxylesterase:->->:766569..766684 116 taaaaaacggcttgattatggaaaaaattgaacaacaccaaagttttggtggcgagcaacaagtttggacgcattttgcccaaactttacaatgcgagatgaaggtggcggtttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01114:AA01115|AA01114:AA01115:alcohol dehydrogenase, class III; glutathione-dependent formaldehyde dehydrogenase:carboxylesterase:->->:766569..766684 116 taaaaaacggcttgattatggaaaaaattgaacaacaccaaagttttggtggcgagcaacaagtttggacgcattttgcccaaactttacaatgcgagatgaaggtggcggtttat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaaaacggcttgattatggaaaaaattgaacaacaccaaagttttggtggcgagcaacaagtttggacgcattttgcccaaactttacaatgcgagatgaaggtggcggtttat Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 12 End: 116 ........... M I M E K I E Q H Q S F G G E Q Q V W T H F A Q T L Q C E M K V A V Y Protein_Len: 33 Strand: - Start: 2 End: 100 . F F P K I I S F I S C C W L K P P S C C T Q V C K A W V K C H S M ................ taaaaaacggcttgattatggaaaaaattgaacaacaccaaagttttggtggcgagcaacaagtttggacgcattttgcccaaactttacaatgcgagatgaaggtggcggtttat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaaaacggcttgattatggaaaaaattgaacaacaccaaagttttggtggcgagcaacaagtttggacgcattttgcccaaactttacaatgcgagatgaaggtggcggtttat Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 74 HP_score: -7.6 Tail_Score: -3.87569 Start: 36 End: 51 Full_Region: aaacttgttgctcgc caccaa aact ttggtg ttgttcaattttttc ...................................caccaaaactttggtg................................................................. Find igs database================================================ Query_seq: AA01114:AA01115|AA01114:AA01115:alcohol dehydrogenase, class III; glutathione-dependent formaldehyde dehydrogenase:carboxylesterase:->->:766569..766684 116 taaaaaacggcttgattatggaaaaaattgaacaacaccaaagttttggtggcgagcaacaagtttggacgcattttgcccaaactttacaatgcgagatgaaggtggcggtttat Intra-Species Hit: Count: 1 Min: 1 Max: 116 Len: 116 Subject: aact_AA01114_AA01115|alcohol dehydrogenase, class III; glutathione-dependent formaldehyde dehydrogenase:carboxylesterase|POSITIVE:POSITIVE|[766569 ,766684]|116 HSP 1 e-value: 8.0E-60 bit: 230.0 Len: 116 Query Start:1 Query End:116 Subject Strand: POSITIVE Subject Start: 1 Subject End: 116 taaaaaacggcttgattatggaaaaaattgaacaacaccaaagttttggtggcgagcaacaagtttggacgcattttgcccaaactttacaatgcgagatgaaggtggcggtttat taaaaaacggcttgattatggaaaaaattgaacaacaccaaagttttggtggcgagcaacaagtttggacgcattttgcccaaactttacaatgcgagatgaaggtggcggtttat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAACGGCUUGAUUAUGGAAAAAAUUGAACAACACCAAAGUUUUGGUGGCGAGCAACAAGUUUGGACGCAUUUUGCCCAAACUUUACAAUGCGAGAUGAAGGUGGCGGUUUAU ....((((.(((...((((................((((((....))))))....(((..((((((((..((.....)))))))))).....)))...))))....))).)))).. (-26.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAACCGCCACCUUCAUCUCGCAUUGUAAAGUUUGGGCAAAAUGCGUCCAAACUUGUUGCUCGCCACCAAAACUUUGGUGUUGUUCAAUUUUUUCCAUAAUCAAGCCGUUUUUUA ..((((.((............(((.....((((((((((.......))))))))))..)))....((((((....))))))........................)).)))).... (-25.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 345 80 42 98 47 aact:AA01170|5end_hypothetical_protein_802464..802694_POSITIVE 315 110 70 53 8 aact:AA02946|5end_virulence_factor_protein_2059216..2059446_POSITIVE 312 80 45 152 111 aact:AA01724|5end_valyl-tRNA_synthetase_1164446..1164676_POSITIVE 312 73 48 33 4 aact:AA01453|5end_D-xylose_isomerase_973457..973687_POSITIVE 310 110 61 75 9 aact:AA01183|5end_conserved_hypothetical_protein_810442..810672_POSITIVE 300 109 66 216 170 aact:AA01475|5end_conserved_hypothetical_protein_(possible_cytoplasmic_protein;_DNA_binding_protein)_990364..990594_POSITIVE 300 109 66 111 65 aact:AA01474|5end_hypothetical_protein_990259..990489_POSITIVE 295 110 66 144 91 aact:AA00915|5end_xanthine-guanine_phosphoribosyltransferase_629610..629840_POSITIVE 292 109 63 180 123 aact:AA02691|5end_hypothetical_protein_1876137..1876367_POSITIVE 290 90 47 205 155 aact:AA01810|5end_electron_transport_complex_protein_1219250..1219480_POSITIVE 290 110 63 91 46 aact:AA00766|5end_heat_shock_protein_B25.3_(HSP-70_cofactor)_527384..527614_POSITIVE 289 110 70 209 155 aact:AA00576|5end_conserved_hypothetical_protein_(possible_translation_factor)_394020..394250_POSITIVE 287 75 44 116 82 aact:AA00292|5end_dihydrofolate_reductase_201800..202030_POSITIVE 286 80 37 168 112 aact:AA01756|5end_cobalt_membrane_transport_protein_1186475..1186705_POSITIVE 286 80 38 202 155 aact:AA01556|5end_ABC_transporter_ATP-binding_protein_1044218..1044448_POSITIVE 283 97 51 80 32 aact:AA01423|5end_cell_division_protein_X,_ABC_system_permease_956668..956898_POSITIVE 282 80 44 156 122 aact:AA01340|5end_possible_lipoprotein;_possible_HlyD_family_secretion_domain_protein;_possible_membrane_fusion_protein_905746..905976_POSITIVE 278 110 64 208 164 aact:AA02560|5end_integral_membrane_protein_1781412..1781642_POSITIVE 276 110 62 191 134 aact:AA01064|5end_conserved_hypothetical_protein_(possible_aminomethyltransferase)_737428..737658_POSITIVE 274 110 70 212 170 aact:AA02030|5end_threonyl-tRNA_synthetase_1382171..1382401_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 307 81 42 120 84 aact:AA02202|3end_transferrin-binding_protein_1_precursor_1500543..1500693_POSITIVE 287 75 44 141 107 aact:AA00291|3end_hypothetical_protein_201905..202055_POSITIVE 287 75 44 49 15 aact:AA00290|3end_conserved_hypothetical_protein_201813..201963_POSITIVE 284 77 36 146 101 aact:AA01021|3end_hypothetical_protein_714276..714426_POSITIVE 283 97 51 68 20 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 282 80 44 127 93 aact:AA01339|3end_transcriptional_regulator,_AcrR/TetR_family_905797..905947_POSITIVE 275 80 31 148 90 aact:AA00993|3end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694999..695149_POSITIVE 273 110 70 62 15 aact:AA00245|3end_hypothetical_protein_173192..173342_POSITIVE 269 79 32 151 111 aact:AA03010|3end_thiophene_and_furan_oxidation_protein_2102534..2102684_POSITIVE 267 79 31 69 28 aact:AA02714|3end_paraquat-inducible_protein_PqiA_homolog_1897167..1897317_POSITIVE 267 81 46 96 55 aact:AA01651|3end_hypothetical_protein_1120004..1120154_POSITIVE 265 97 51 133 93 aact:AA02204|3end_hypothetical_protein_1501262..1501412_POSITIVE 262 110 64 114 80 aact:AA02740|3end_conserved_hypothetical_protein_1921394..1921544_POSITIVE 260 97 51 143 99 aact:AA00611|3end_(3R)-hydroxymyristoyl-acyl_carrier_protein_dehydrase_417896..418046_POSITIVE 257 110 64 48 6 aact:AA02965|3end_hypothetical_protein_2074286..2074436_POSITIVE 257 84 45 147 100 aact:AA01426|3end_threonine_deaminase_(dehydratase)_958655..958805_POSITIVE 256 110 66 117 76 aact:AA00886|3end_hypothetical_protein_616855..617005_POSITIVE 255 110 61 144 87 aact:AA01143|3end_hypothetical_protein_783535..783685_POSITIVE 252 79 42 143 111 aact:AA02010|3end_peptide_methionine_sulfoxide_reductase;_cytochrome_C-TYPE_biogenesis_protein;_transcriptional_regulator_1367739..1367889_POSITIVE 252 80 43 56 11 aact:AA01240|3end_30S_ribosomal_protein_S11_845341..845491_POSITIVE