Origin IGS: ggttttctcctttttcatgttggtcggcggtgccgattacctttaaactataacagaaaaatgctatattagcgcccgatttcccgatgactcaattcaatttaaaacccca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tggggttttaaattgaattgagtcatcgggaaatcgggcgctaatatagcatttttctgttatagtttaaaggtaatcggcaccgccgaccaacatgaaaaaggagaaaacc Mask Tandem Repeat Region ================================================ ggttttctcctttttcatgttggtcggcggtgccgattacctttaaactataacagaaaaatgctatattagcgcccgatttcccgatgactcaattcaatttaaaacccca Find is-nt database================================================ Query_seq: AA01104:AA01106|AA01104:AA01106:conserved hypothetical protein:conserved hypothetical protein:->->:761703..761814 112 ggttttctcctttttcatgttggtcggcggtgccgattacctttaaactataacagaaaaatgctatattagcgcccgatttcccgatgactcaattcaatttaaaacccca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01104:AA01106|AA01104:AA01106:conserved hypothetical protein:conserved hypothetical protein:->->:761703..761814 112 ggttttctcctttttcatgttggtcggcggtgccgattacctttaaactataacagaaaaatgctatattagcgcccgatttcccgatgactcaattcaatttaaaacccca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01104:AA01106|AA01104:AA01106:conserved hypothetical protein:conserved hypothetical protein:->->:761703..761814 112 ggttttctcctttttcatgttggtcggcggtgccgattacctttaaactataacagaaaaatgctatattagcgcccgatttcccgatgactcaattcaatttaaaacccca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggttttctcctttttcatgttggtcggcggtgccgattacctttaaactataacagaaaaatgctatattagcgcccgatttcccgatgactcaattcaatttaaaacccca Predict ORF larger than 30AA ================================================ ggttttctcctttttcatgttggtcggcggtgccgattacctttaaactataacagaaaaatgctatattagcgcccgatttcccgatgactcaattcaatttaaaacccca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggttttctcctttttcatgttggtcggcggtgccgattacctttaaactataacagaaaaatgctatattagcgcccgatttcccgatgactcaattcaatttaaaacccca Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01104:AA01106|AA01104:AA01106:conserved hypothetical protein:conserved hypothetical protein:->->:761703..761814 112 ggttttctcctttttcatgttggtcggcggtgccgattacctttaaactataacagaaaaatgctatattagcgcccgatttcccgatgactcaattcaatttaaaacccca Intra-Species Hit: Count: 1 Min: 1 Max: 112 Len: 112 Subject: aact_AA01104_AA01106|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[761703,761814]|112 HSP 1 e-value: 2.0E-57 bit: 222.0 Len: 112 Query Start:1 Query End:112 Subject Strand: POSITIVE Subject Start: 1 Subject End: 112 ggttttctcctttttcatgttggtcggcggtgccgattacctttaaactataacagaaaaatgctatattagcgcccgatttcccgatgactcaattcaatttaaaacccca ggttttctcctttttcatgttggtcggcggtgccgattacctttaaactataacagaaaaatgctatattagcgcccgatttcccgatgactcaattcaatttaaaacccca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGUUUUCUCCUUUUUCAUGUUGGUCGGCGGUGCCGAUUACCUUUAAACUAUAACAGAAAAAUGCUAUAUUAGCGCCCGAUUUCCCGAUGACUCAAUUCAAUUUAAAACCCCA ((((((....((((((.(((((((((((...)))))).............))))))))))).((((...))))..............................))))))... (-18.21) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGGGGUUUUAAAUUGAAUUGAGUCAUCGGGAAAUCGGGCGCUAAUAUAGCAUUUUUCUGUUAUAGUUUAAAGGUAAUCGGCACCGCCGACCAACAUGAAAAAGGAGAAAACC ...((((((...((((((((......(((((((....((.........))..)))))))...)))))))).((...(((((...)))))))...............)))))) (-20.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 367 48 9 222 175 aact:AA01657|5end_maltose_high-affinity_receptor_1122735..1122965_POSITIVE 357 41 5 132 97 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 318 59 19 81 44 aact:AA00093|5end_L-serine_dehydratase_(L-serine_deaminase)_69732..69962_POSITIVE 310 41 1 154 112 aact:AA02972|5end_conserved_hypothetical_protein_(tyrosine_phenol_lyase-related_protein)_2077381..2077611_POSITIVE 309 36 10 66 40 aact:AA00471|5end_3,4-dihydroxy-2-butanone_4-phosphate_synthase;_GTP_cyclohydrase_II_328262..328492_POSITIVE 308 34 5 136 114 aact:AA00864|5end_conserved_hypothetical_protein_598605..598835_POSITIVE 303 36 6 72 41 aact:AA00636|5end_MrsA_protein_(phosphoglucomutase/phosphomannomutase)_436780..437010_POSITIVE 295 35 10 121 96 aact:AA02567|5end_hypothetical_protein_(possible_inner_membrane_protein)_1788225..1788455_POSITIVE 295 78 61 133 116 aact:AA02015|5end_phosphoheptose_isomerase_1371059..1371289_POSITIVE 287 46 1 168 127 aact:AA01523|5end_conserved_hypothetical_protein_1023205..1023435_POSITIVE 285 56 16 76 28 aact:AA01708|5end_glutaredoxin_1158136..1158366_POSITIVE 285 35 17 65 42 aact:AA01614|5end_D-isomer_specific_2-hydroxyacid_dehydrogenase_family_protein_1089698..1089928_POSITIVE 285 35 7 180 150 aact:AA01393|5end_ABC_transporter,_ATP-binding_protein_940369..940599_POSITIVE 283 109 64 76 19 aact:AA01301|5end_competence_protein_B_881467..881697_POSITIVE 282 44 8 133 97 aact:AA00190|5end_triosephosphate_isomerase_134088..134318_POSITIVE 280 93 53 194 158 aact:AA02063|5end_23S_rRNA_psedouridine_synthase,_Rlu_family_protein_1404451..1404681_POSITIVE 278 40 1 173 132 aact:AA00398|5end_protein-tyrosine-phosphatase_(PTPase)_267447..267677_POSITIVE 275 43 1 228 181 aact:AA00312|5end_conserved_hypothetical_protein_(possible_hydroxypyruvate_isomerase)_212069..212299_POSITIVE 273 43 2 211 152 aact:AA02568|5end_sugar_phosphotransferase_component_II_B_1788379..1788609_POSITIVE 273 38 1 199 160 aact:AA02378|5end_hypothetical_protein_1627753..1627983_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 367 48 9 142 95 aact:AA01656|3end_maltose/maltodextrin_transport_ATP-binding_protein_1122735..1122885_POSITIVE 357 41 5 126 91 aact:AA00332|3end_PTS_system,_fructose-specific_IIA/FPr_component_(diphosphoryl_transfer_protein)_225617..225767_POSITIVE 310 41 1 84 42 aact:AA02970|3end_tyrosine_phenol-lyase_2077391..2077541_POSITIVE 308 34 5 129 107 aact:AA00863|3end_conserved_hypothetical_protein_598678..598828_POSITIVE 303 36 6 40 9 aact:AA00635|3end_dihydropteroate_synthase_436828..436978_POSITIVE 297 45 1 146 93 aact:AA02340|3end_hypothetical_protein_1599341..1599491_POSITIVE 285 35 7 133 103 aact:AA01391|3end_glycerol-3-phosphate_acyltransferase_940402..940552_POSITIVE 280 93 53 75 39 aact:AA02062|3end_conserved_hypothetical_protein_(probable_membrane_protein)_1404412..1404562_POSITIVE 278 40 1 135 94 aact:AA00397|3end_Fe-S_oxidoreductase;_MiaB-like_RNA_modifying_enzyme_(2-methylthioadenine_synthetase)_267489..267639_POSITIVE 273 38 1 108 69 aact:AA02375|3end_D-3-phosphoglycerate_dehydrogenase_1627742..1627892_POSITIVE 273 41 2 127 70 aact:AA02374|3end_ribose_5-phosphate_isomerase_A_1626572..1626722_POSITIVE 272 41 8 47 21 aact:AA02204|3end_hypothetical_protein_1501262..1501412_POSITIVE 271 49 16 77 41 aact:AA02801|3end_myo-inositol_catabolism_protein_1969073..1969223_POSITIVE 270 34 1 151 121 aact:AA01522|3end_conserved_hypothetical_protein_1023279..1023429_POSITIVE 270 36 1 112 78 aact:AA00658|3end_hypothetical_protein_451952..452102_POSITIVE 269 100 60 138 94 aact:AA01254|3end_conserved_hypothetical_protein_(possible_proline_dipeptidase)_852702..852852_POSITIVE 269 36 8 64 34 aact:AA00573|3end_chloramphenicol-sensitive_protein_RarD_393295..393445_POSITIVE 266 37 1 147 104 aact:AA00335|3end_PTS_fructose-specific_IIBC_component_228230..228380_POSITIVE 263 110 67 142 103 aact:AA00894|3end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_621350..621500_POSITIVE 262 37 17 38 17 aact:AA01950|3end_ABC_transporter,_membrane_spanning_protein_1319044..1319194_POSITIVE