Origin IGS: tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaacgtaatttgagtgtcattgggcgatagccatctcgttttctgttccttttataccgcaacaagatagtttcttttatctcacgataatcgtgtttaacttgctgataataaaagatagaacgaggcattttagccacacgaagtaagtcattcaacgcgtgatttggcttca Mask Tandem Repeat Region ================================================ tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Find is-nt database================================================ Query_seq: AA01075:AA01076|AA01075:AA01076:hypothetical protein:hypothetical protein:->->:746129..746304 176 tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01075:AA01076|AA01075:AA01076:hypothetical protein:hypothetical protein:->->:746129..746304 176 tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01075:AA01076|AA01075:AA01076:hypothetical protein:hypothetical protein:->->:746129..746304 176 tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Predict ORF larger than 30AA ================================================ Protein_Len: 53 Strand: + Start: 18 End: 173 ................. M N D L L R V A K M P R S I F Y Y Q Q V K H D Y R E I K E T I L L R Y K R N R K R D G Y R P M T L K L R * ... Protein_Len: 40 Strand: - Start: 30 End: 149 ............................. K T H S F H R T R D K I I L L N F V I I T L Y F F S D Q Q P I F P V S F S I A M ........................... tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 83 Start: 17 End: 46 ................GTTGAATGACTTACTTCGTGTGGCTAAAAT.................................................................................................................................. Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 72 HP_score: -7.7 Tail_Score: -3.79043 Start: 81 End: 93 Full_Region: tagtttcttttatct cacga taa tcgtg tttaacttgctgata ................................................................................cacgataatcgtg................................................................................... Find igs database================================================ Query_seq: AA01075:AA01076|AA01075:AA01076:hypothetical protein:hypothetical protein:->->:746129..746304 176 tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Intra-Species Hit: Count: 4 Min: 1 Max: 176 Len: 176 Subject: aact_AA02340_AA02341|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1599402,1599585]|184 HSP 1 e-value: 2.0E-95 bit: 349.0 Len: 176 Query Start:1 Query End:176 Subject Strand: POSITIVE Subject Start: 9 Subject End: 184 tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Subject: aact_AA02115_AA02116|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[1445052,1445227]|176 HSP 1 e-value: 2.0E-95 bit: 349.0 Len: 176 Query Start:1 Query End:176 Subject Strand: POSITIVE Subject Start: 1 Subject End: 176 tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Subject: aact_AA01288_AA01289|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[872349,872524]|176 HSP 1 e-value: 2.0E-95 bit: 349.0 Len: 176 Query Start:1 Query End:176 Subject Strand: NEGATIVE Subject Start: 1 Subject End: 176 tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Subject: aact_AA01075_AA01076|hypothetical protein:hypothetical protein|POSITIVE:POSITIVE|[746129,746304]|176 HSP 1 e-value: 2.0E-95 bit: 349.0 Len: 176 Query Start:1 Query End:176 Subject Strand: POSITIVE Subject Start: 1 Subject End: 176 tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa tgaagccaaatcacgcgttgaatgacttacttcgtgtggctaaaatgcctcgttctatcttttattatcagcaagttaaacacgattatcgtgagataaaagaaactatcttgttgcggtataaaaggaacagaaaacgagatggctatcgcccaatgacactcaaattacgttaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAGCCAAAUCACGCGUUGAAUGACUUACUUCGUGUGGCUAAAAUGCCUCGUUCUAUCUUUUAUUAUCAGCAAGUUAAACACGAUUAUCGUGAGAUAAAAGAAACUAUCUUGUUGCGGUAUAAAAGGAACAGAAAACGAGAUGGCUAUCGCCCAAUGACACUCAAAUUACGUUAA .(((((((..(((((((...............))))))).........((((((((.((((((((...(((((((.......(..(((((....)))))..).......)))))))....))))))))...)))..))))).))))).)).......................... (-40.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAACGUAAUUUGAGUGUCAUUGGGCGAUAGCCAUCUCGUUUUCUGUUCCUUUUAUACCGCAACAAGAUAGUUUCUUUUAUCUCACGAUAAUCGUGUUUAACUUGCUGAUAAUAAAAGAUAGAACGAGGCAUUUUAGCCACACGAAGUAAGUCAUUCAACGCGUGAUUUGGCUUCA .....((.....(((((((....(((....)))...((((..(((((..(((((((.(.((((..((((((......))))))((((.....))))......)))).)...))))))))))))))))))))))).....))..((((((((((((.......))))))).))))). (-41.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 926 60 11 151 102 aact:AA01289|5end_hypothetical_protein_872385..872615_POSITIVE 410 70 21 161 112 aact:AA00227|5end_hypothetical_sequence_156407..156637_POSITIVE 359 154 111 62 6 aact:AA01291|5end_excinuclease_ABC_subunit_A_872829..873059_POSITIVE 349 88 43 43 3 aact:AA00226|5end_hypothetical_protein_156276..156506_POSITIVE 333 57 14 210 152 aact:AA00207|5end_D-ribose_ABC_transporter,_ATP-binding_protein_/_galactoside_ABC_transporter,_ATP-binding_protein_142592..142822_POSITIVE 318 155 112 203 159 aact:AA02933|5end_conserved_hypothetical_protein_(HesA/MoeB/ThiF_family_protein)_2053467..2053697_POSITIVE 296 160 115 148 95 aact:AA01411|5end_stationary-phase_survival_protein_951000..951230_POSITIVE 290 53 12 222 176 aact:AA00326|5end_periplasmic_oxidoreductase_(por)_(Thiol:disulfide_interchange_protein_dsbA_precursor.)_221703..221933_POSITIVE 289 38 5 46 5 aact:AA02564|5end_transketolase_N-terminal_section_1786173..1786403_POSITIVE 286 59 14 52 4 aact:AA02190|5end_hydrogenase-2_component_protein_1492919..1493149_POSITIVE 284 39 5 114 72 aact:AA01846|5end_conserved_hypothetical_protein_1241843..1242073_POSITIVE 281 59 15 208 169 aact:AA02566|5end_PTS_system,_IIC_component_1787008..1787238_POSITIVE 280 159 116 226 178 aact:AA00013|5end_hypothetical_protein_6695..6925_POSITIVE 275 160 111 192 142 aact:AA01054|5end_conserved_hypothetical_protein_733678..733908_POSITIVE 270 170 126 123 81 aact:AA01279|5end_phage_exclusion-related_protein_867687..867917_POSITIVE 269 57 11 105 48 aact:AA00792|5end_conserved_hypothetical_protein_543142..543372_POSITIVE 268 154 111 103 63 aact:AA01117|5end_signal_recognition_particle_protein_54_(GTP-binding_export_factor)_767919..768149_POSITIVE 268 49 5 183 149 aact:AA00042|5end_hypothetical_protein_33810..34040_POSITIVE 267 41 4 71 41 aact:AA01231|5end_30S_ribosomal_protein_S14_840103..840333_POSITIVE 266 158 111 177 136 aact:AA00790|5end_recombination_DNA_repair_protein_542455..542685_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 916 160 111 74 25 aact:AA01288|3end_hypothetical_protein_872288..872438_POSITIVE 410 70 21 125 76 aact:AA00226|3end_hypothetical_protein_156451..156601_POSITIVE 296 160 115 117 64 aact:AA01409|3end_lipoprotein_B_951049..951199_POSITIVE 289 38 5 53 12 aact:AA02563|3end_transketolase_C-terminal_section_1786260..1786410_POSITIVE 270 170 126 81 39 aact:AA01278|3end_hypothetical_protein_867725..867875_POSITIVE 268 49 5 110 76 aact:AA00041|3end_cytochrome_c-type_biogenesis_protein_33817..33967_POSITIVE 267 41 4 55 25 aact:AA01230|3end_50S_ribosomal_protein_L5_840167..840317_POSITIVE 265 155 111 113 69 aact:AA01547|3end_deoxyribose-phosphate_aldolase_1037019..1037169_POSITIVE 263 41 4 151 106 aact:AA00325|3end_conserved_hypothetical_protein_221720..221870_POSITIVE 262 50 10 58 24 aact:AA01741|3end_6-pyruvoyl_tetrahydrobiopterin_synthase_1177695..1177845_POSITIVE 259 49 5 135 93 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 257 93 55 48 15 aact:AA00279|3end_8-amino-7-oxonoanoate_synthase_196514..196664_POSITIVE 256 147 101 149 84 aact:AA00822|3end_conserved_hypothetical_protein_(possible_arsenate_reductase)_565017..565167_POSITIVE 255 167 137 120 80 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 255 52 14 143 100 aact:AA00794|3end_phosphohydrolase_544800..544950_POSITIVE 254 159 114 119 69 aact:AA00954|3end_uroporphyrinogen_III_synthase_661070..661220_POSITIVE 253 60 15 49 8 aact:AA02629|3end_hypothetical_protein_1830274..1830424_POSITIVE 253 59 11 83 31 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 252 40 5 151 106 aact:AA02426|3end_uridylyltransferase_1674226..1674376_POSITIVE 251 154 111 100 48 aact:AA01078|3end_transcriptional_regulator_(probable_MerR_family)_747453..747603_POSITIVE