Origin IGS: taacatggcattactttacagcggaaccaaaccgaaaaaaaataccgccaatttcaccgcccacatccttgatttggattaccaggggctgggcgtagcgaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tttcgctacgcccagcccctggtaatccaaatcaaggatgtgggcggtgaaattggcggtattttttttcggtttggttccgctgtaaagtaatgccatgtta Mask Tandem Repeat Region ================================================ taacatggcattactttacagcggaaccaaaccgaaaaaaaataccgccaatttcaccgcccacatccttgatttggattaccaggggctgggcgtagcgaaa Find is-nt database================================================ Query_seq: AA01070:AA01071|AA01070:AA01071:DNA repair protein:RNA methyltransferase:->->:741744..741846 103 taacatggcattactttacagcggaaccaaaccgaaaaaaaataccgccaatttcaccgcccacatccttgatttggattaccaggggctgggcgtagcgaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01070:AA01071|AA01070:AA01071:DNA repair protein:RNA methyltransferase:->->:741744..741846 103 taacatggcattactttacagcggaaccaaaccgaaaaaaaataccgccaatttcaccgcccacatccttgatttggattaccaggggctgggcgtagcgaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01070:AA01071|AA01070:AA01071:DNA repair protein:RNA methyltransferase:->->:741744..741846 103 taacatggcattactttacagcggaaccaaaccgaaaaaaaataccgccaatttcaccgcccacatccttgatttggattaccaggggctgggcgtagcgaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taacatggcattactttacagcggaaccaaaccgaaaaaaaataccgccaatttcaccgcccacatccttgatttggattaccaggggctgggcgtagcgaaa Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 5 End: 103 .... M A L L Y S G T K P K K N T A N F T A H I L D L D Y Q G L G V A K taacatggcattactttacagcggaaccaaaccgaaaaaaaataccgccaatttcaccgcccacatccttgatttggattaccaggggctgggcgtagcgaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taacatggcattactttacagcggaaccaaaccgaaaaaaaataccgccaatttcaccgcccacatccttgatttggattaccaggggctgggcgtagcgaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01070:AA01071|AA01070:AA01071:DNA repair protein:RNA methyltransferase:->->:741744..741846 103 taacatggcattactttacagcggaaccaaaccgaaaaaaaataccgccaatttcaccgcccacatccttgatttggattaccaggggctgggcgtagcgaaa Intra-Species Hit: Count: 1 Min: 1 Max: 103 Len: 103 Subject: aact_AA01070_AA01071|DNA repair protein:RNA methyltransferase|POSITIVE:POSITIVE|[741744,741846]|103 HSP 1 e-value: 8.0E-38 bit: 157.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 1 Subject End: 103 taacatggcattactttacagcggaaccaaaccgnnnnnnnntaccgccaatttcaccgcccacatccttgatttggattaccaggggctgggcgtagcgaaa taacatggcattactttacagcggaaccaaaccgaaaaaaaataccgccaatttcaccgcccacatccttgatttggattaccaggggctgggcgtagcgaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAACAUGGCAUUACUUUACAGCGGAACCAAACCGAAAAAAAAUACCGCCAAUUUCACCGCCCACAUCCUUGAUUUGGAUUACCAGGGGCUGGGCGUAGCGAAA .....((((............(((.......)))............))))..(((.(((((((..((((((...........)))))).))))))..).))). (-24.65) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUCGCUACGCCCAGCCCCUGGUAAUCCAAAUCAAGGAUGUGGGCGGUGAAAUUGGCGGUAUUUUUUUUCGGUUUGGUUCCGCUGUAAAGUAAUGCCAUGUUA ((((((..((((((..((.((((.......)))).))...)))))))))))).(((((.((((((....(((.......)))....)))))).)))))..... (-31.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 437 99 52 177 126 aact:AA00533|5end_conserved_hypothetical_protein_365323..365553_POSITIVE 382 95 56 130 83 aact:AA00525|5end_hypothetical_protein_359254..359484_POSITIVE 367 99 57 180 129 aact:AA02565|5end_transketolase,_N-terminal_subunit_1786366..1786596_POSITIVE 359 90 44 193 140 aact:AA02496|5end_hypothetical_protein_1731960..1732190_POSITIVE 355 99 55 53 10 aact:AA00729|5end_possible_hemolysin_(TlyC-like)_500204..500434_POSITIVE 350 87 45 69 31 aact:AA01679|5end_aspartate-semialdehyde_dehydrogenase;_PTS_system_enzyme_II_A_component_1134356..1134586_POSITIVE 349 100 55 102 49 aact:AA02297|5end_conserved_hypothetical_protein_1569620..1569850_POSITIVE 349 100 55 102 49 aact:AA00621|5end_conserved_hypothetical_protein_428727..428957_POSITIVE 349 100 55 102 49 aact:AA00412|5end_conserved_hypothetical_protein_279174..279404_POSITIVE 343 99 54 175 130 aact:AA00259|5end_conserved_hypothetical_protein_182788..183018_POSITIVE 341 100 57 205 158 aact:AA00500|5end_hypothetical_protein_347151..347381_POSITIVE 340 100 57 197 155 aact:AA02696|5end_formate_hydrogenlyase_maturation_protein_1879476..1879706_POSITIVE 336 90 42 51 5 aact:AA01732|5end_multidrug_resistance_protein_A_1170677..1170907_POSITIVE 333 98 52 168 128 aact:AA02344|5end_GTP-binding_protein,_elongation_factor_typA/bipA_1601966..1602196_POSITIVE 328 100 57 186 134 aact:AA01813|5end_electron_transport_complex_protein_1220949..1221179_POSITIVE 328 100 54 203 151 aact:AA00341|5end_hypothetical_protein_229388..229618_POSITIVE 326 99 58 155 114 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 319 69 32 42 1 aact:AA01660|5end_hypothetical_protein_1125035..1125265_POSITIVE 318 99 51 199 142 aact:AA01900|5end_hypothetical_protein_1282233..1282463_POSITIVE 314 100 51 140 66 aact:AA02747|5end_ribose-phosphate_pyrophosphokinase;_phosphoribosylpyrophosphate_synthase_1924324..1924554_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 359 90 44 145 92 aact:AA02495|3end_conserved_hypothetical_protein_1731992..1732142_POSITIVE 348 85 48 147 103 aact:AA00531|3end_DNA_polymerase_I_365385..365535_POSITIVE 342 99 57 142 103 aact:AA01220|3end_competence_protein_F;_amidophosphoribosyltransferase_833538..833688_POSITIVE 340 100 57 59 17 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 326 99 58 140 99 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 312 89 46 144 106 aact:AA01561|3end_conserved_hypothetical_protein_1046971..1047121_POSITIVE 310 99 54 53 2 aact:AA00969|3end_conserved_hypothetical_protein_(possible_NADH_pyrophosphatase)_676206..676356_POSITIVE 299 99 54 149 76 aact:AA02157|3end_TonB-dependent_outer_membrane_receptor_1476305..1476455_POSITIVE 297 100 52 96 16 aact:AA01818|3end_high-affinity_zinc_uptake_system_membrane_protein_1225078..1225228_POSITIVE 297 99 62 49 7 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 294 90 46 148 97 aact:AA00340|3end_NADP(H)_oxidoreductase_(NAD(P)H_dehydrogenase_(quinone))_229425..229575_POSITIVE 292 99 54 85 22 aact:AA00170|3end_hypothetical_protein_121407..121557_POSITIVE 289 89 46 56 9 aact:AA00514|3end_hypothetical_protein_355838..355988_POSITIVE 288 99 52 88 22 aact:AA00989|3end_hypothetical_protein_692644..692794_POSITIVE 285 75 31 89 46 aact:AA01519|3end_conserved_hypothetical_protein_1022539..1022689_POSITIVE 285 83 47 141 109 aact:AA01062|3end_ribonuclease_PH_(RNase_PH)_736499..736649_POSITIVE 282 100 58 141 87 aact:AA00041|3end_cytochrome_c-type_biogenesis_protein_33817..33967_POSITIVE 281 95 54 50 8 aact:AA02677|3end_formate_dehydrogenase,_beta_subunit_1865105..1865255_POSITIVE 279 90 44 107 45 aact:AA00601|3end_hypothetical_protein_409119..409269_POSITIVE 279 99 58 74 24 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE