Origin IGS: taattgatgatttaagcaaaaaagcaggtgtcaaaacctgcttttttattctccggcatttctcgattctcacgcttgcccgatagttcaagcgtccacgcttagaccgataaaatagcacagacctgaacatttacactaccatcaatcagtgccgacatttccgttataacccattcccttttattctaaaatcccacgcaaaaatcccacaaagtgcggtgtgaaatctgctagaataagcgcaattttttatcggcgaaaagggcgacatcatcgaaaattggcaacgcggtttc .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 gaaaccgcgttgccaattttcgatgatgtcgcccttttcgccgataaaaaattgcgcttattctagcagatttcacaccgcactttgtgggatttttgcgtgggattttagaataaaagggaatgggttataacggaaatgtcggcactgattgatggtagtgtaaatgttcaggtctgtgctattttatcggtctaagcgtggacgcttgaactatcgggcaagcgtgagaatcgagaaatgccggagaataaaaaagcaggttttgacacctgcttttttgcttaaatcatcaatta Mask Tandem Repeat Region ================================================ taattgatgatttaagcaaaaaagcaggtgtcaaaacctgcttttttattctccggcatttctcgattctcacgcttgcccgatagttcaagcgtccacgcttagaccgataaaatagcacagacctgaacatttacactaccatcaatcagtgccgacatttccgttataacccattcccttttattctaaaatcccacgcaaaaatcccacaaagtgcggtgtgaaatctgctagaataagcgcaattttttatcggcgaaaagggcgacatcatcgaaaattggcaacgcggtttc Find is-nt database================================================ Query_seq: AA01069:AA01070|AA01069:AA01070:translation elongation factor (EF-Ts):DNA repair protein:->->:740761..741059 299 taattgatgatttaagcaaaaaagcaggtgtcaaaacctgcttttttattctccggcatttctcgattctcacgcttgcccgatagttcaagcgtccacgcttagaccgataaaatagcacagacctgaacatttacactaccatcaatcagtgccgacatttccgttataacccattcccttttattctaaaatcccacgcaaaaatcccacaaagtgcggtgtgaaatctgctagaataagcgcaattttttatcggcgaaaagggcgacatcatcgaaaattggcaacgcggtttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01069:AA01070|AA01069:AA01070:translation elongation factor (EF-Ts):DNA repair protein:->->:740761..741059 299 taattgatgatttaagcaaaaaagcaggtgtcaaaacctgcttttttattctccggcatttctcgattctcacgcttgcccgatagttcaagcgtccacgcttagaccgataaaatagcacagacctgaacatttacactaccatcaatcagtgccgacatttccgttataacccattcccttttattctaaaatcccacgcaaaaatcccacaaagtgcggtgtgaaatctgctagaataagcgcaattttttatcggcgaaaagggcgacatcatcgaaaattggcaacgcggtttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01069:AA01070|AA01069:AA01070:translation elongation factor (EF-Ts):DNA repair protein:->->:740761..741059 299 taattgatgatttaagcaaaaaagcaggtgtcaaaacctgcttttttattctccggcatttctcgattctcacgcttgcccgatagttcaagcgtccacgcttagaccgataaaatagcacagacctgaacatttacactaccatcaatcagtgccgacatttccgttataacccattcccttttattctaaaatcccacgcaaaaatcccacaaagtgcggtgtgaaatctgctagaataagcgcaattttttatcggcgaaaagggcgacatcatcgaaaattggcaacgcggtttc Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattgatgatttaagcaaaaaagcaggtgtcaaaacctgcttttttattctccggcatttctcgattctcacgcttgcccgatagttcaagcgtccacgcttagaccgataaaatagcacagacctgaacatttacactaccatcaatcagtgccgacatttccgttataacccattcccttttattctaaaatcccacgcaaaaatcccacaaagtgcggtgtgaaatctgctagaataagcgcaattttttatcggcgaaaagggcgacatcatcgaaaattggcaacgcggtttc Predict ORF larger than 30AA ================================================ Protein_Len: 33 Strand: + Start: 126 End: 224 ............................................................................................................................. M N I Y T T I N Q C R H F R Y N P F P F I L K S H A K I P Q S A V ........................................................................... Protein_Len: 41 Strand: + Start: 176 End: 298 ............................................................................................................................................................................... M P F Y S K I P R K N P T K C G V K S A R I S A I F Y R R K G R H H R K L A T R F . Protein_Len: 36 Strand: - Start: 170 End: 277 ......................................................................................................................................................................... L G N G K I R F D W A F I G C L A T H F R S S Y A C N K I P S F P S M M ...................... Protein_Len: 44 Strand: - Start: 2 End: 133 . I S S K L L F A P T L V Q K K I R R C K E I R V S A R Y N L R G R K S R Y F L V S R F M ...................................................................................................................................................................... taattgatgatttaagcaaaaaagcaggtgtcaaaacctgcttttttattctccggcatttctcgattctcacgcttgcccgatagttcaagcgtccacgcttagaccgataaaatagcacagacctgaacatttacactaccatcaatcagtgccgacatttccgttataacccattcccttttattctaaaatcccacgcaaaaatcccacaaagtgcggtgtgaaatctgctagaataagcgcaattttttatcggcgaaaagggcgacatcatcgaaaattggcaacgcggtttc Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattgatgatttaagcaaaaaagcaggtgtcaaaacctgcttttttattctccggcatttctcgattctcacgcttgcccgatagttcaagcgtccacgcttagaccgataaaatagcacagacctgaacatttacactaccatcaatcagtgccgacatttccgttataacccattcccttttattctaaaatcccacgcaaaaatcccacaaagtgcggtgtgaaatctgctagaataagcgcaattttttatcggcgaaaagggcgacatcatcgaaaattggcaacgcggtttc Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 89 HP_score: -7.0 Tail_Score: -5.28481 Start: 24 End: 41 Full_Region: ccggagaataaaaaa gcaggt tttgac acctgc ttttttgcttaaatc .......................gcaggttttgacacctgc.................................................................................................................................................................................................................................................................. Find igs database================================================ Query_seq: AA01069:AA01070|AA01069:AA01070:translation elongation factor (EF-Ts):DNA repair protein:->->:740761..741059 299 taattgatgatttaagcaaaaaagcaggtgtcaaaacctgcttttttattctccggcatttctcgattctcacgcttgcccgatagttcaagcgtccacgcttagaccgataaaatagcacagacctgaacatttacactaccatcaatcagtgccgacatttccgttataacccattcccttttattctaaaatcccacgcaaaaatcccacaaagtgcggtgtgaaatctgctagaataagcgcaattttttatcggcgaaaagggcgacatcatcgaaaattggcaacgcggtttc Intra-Species Hit: Count: 1 Min: 1 Max: 299 Len: 299 Subject: aact_AA01069_AA01070|translation elongation factor (EF-Ts):DNA repair protein|POSITIVE:POSITIVE|[740761,741059]|299 HSP 1 e-value: 1.0E-168 bit: 593.0 Len: 299 Query Start:1 Query End:299 Subject Strand: POSITIVE Subject Start: 1 Subject End: 299 taattgatgatttaagcaaaaaagcaggtgtcaaaacctgcttttttattctccggcatttctcgattctcacgcttgcccgatagttcaagcgtccacgcttagaccgataaaatagcacagacctgaacatttacactaccatcaatcagtgccgacatttccgttataacccattcccttttattctaaaatcccacgcaaaaatcccacaaagtgcggtgtgaaatctgctagaataagcgcaattttttatcggcgaaaagggcgacatcatcgaaaattggcaacgcggtttc taattgatgatttaagcaaaaaagcaggtgtcaaaacctgcttttttattctccggcatttctcgattctcacgcttgcccgatagttcaagcgtccacgcttagaccgataaaatagcacagacctgaacatttacactaccatcaatcagtgccgacatttccgttataacccattcccttttattctaaaatcccacgcaaaaatcccacaaagtgcggtgtgaaatctgctagaataagcgcaattttttatcggcgaaaagggcgacatcatcgaaaattggcaacgcggtttc Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUGAUGAUUUAAGCAAAAAAGCAGGUGUCAAAACCUGCUUUUUUAUUCUCCGGCAUUUCUCGAUUCUCACGCUUGCCCGAUAGUUCAAGCGUCCACGCUUAGACCGAUAAAAUAGCACAGACCUGAACAUUUACACUACCAUCAAUCAGUGCCGACAUUUCCGUUAUAACCCAUUCCCUUUUAUUCUAAAAUCCCACGCAAAAAUCCCACAAAGUGCGGUGUGAAAUCUGCUAGAAUAAGCGCAAUUUUUUAUCGGCGAAAAGGGCGACAUCAUCGAAAAUUGGCAACGCGGUUUC ...((((((((..((((.(((((((((((......))))))))))).......(((......)))........))))((((........(((((....))))).(.(((((((((..((((.((..(((...............))).)).))))...........................(((((((((........((((.....(((((...))).))))))........))))))))).......)))))))))).....))))...)))))))).....(....)........ (-66.15) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GAAACCGCGUUGCCAAUUUUCGAUGAUGUCGCCCUUUUCGCCGAUAAAAAAUUGCGCUUAUUCUAGCAGAUUUCACACCGCACUUUGUGGGAUUUUUGCGUGGGAUUUUAGAAUAAAAGGGAAUGGGUUAUAACGGAAAUGUCGGCACUGAUUGAUGGUAGUGUAAAUGUUCAGGUCUGUGCUAUUUUAUCGGUCUAAGCGUGGACGCUUGAACUAUCGGGCAAGCGUGAGAAUCGAGAAAUGCCGGAGAAUAAAAAAGCAGGUUUUGACACCUGCUUUUUUGCUUAAAUCAUCAAUUA ..........((((.((((((((((((.(..(((((((.((((((.....)))).))..(((((((((((..((...((((.....))))))..))))).))))))........)))))))...).)))))..)))))))...)))).(((((((((((................((((.((.(((((.(((((((..((((....((((((....))))))..)))).)).))))))))))))))))...(((((((((((((......))))))))))))).....))))))))))) (-88.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 405 225 190 166 131 aact:AA00501|5end_hypothetical_protein_347293..347523_POSITIVE 375 102 62 105 62 aact:AA01995|5end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355120..1355350_POSITIVE 370 99 53 109 61 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 363 113 72 227 191 aact:AA01623|5end_alanine_racemase_1_1097077..1097307_POSITIVE 357 260 213 179 127 aact:AA01544|5end_conserved_hypothetical_protein_1034265..1034495_POSITIVE 357 260 213 89 37 aact:AA01543|5end_hypothetical_protein_1034175..1034405_POSITIVE 343 99 54 66 16 aact:AA00163|5end_type_IV_prepilin_peptidase;_type_II_secretion_pathway_related_protein_116006..116236_POSITIVE 340 236 196 72 24 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 337 120 76 167 127 aact:AA01890|5end_cell-cycle_protein_1274544..1274774_POSITIVE 336 280 231 72 13 aact:AA02495|5end_conserved_hypothetical_protein_1731562..1731792_POSITIVE 333 109 61 230 182 aact:AA00377|5end_acetyl-CoA_acetyltransferase_(Beta-ketothiolase,3-ketoacyl-CoA_thiolase)_253042..253272_POSITIVE 330 250 208 71 29 aact:AA00608|5end_protective_surface_antigen_D-15,_85_-kilodalton_outermembrane_protein_413161..413391_POSITIVE 324 259 212 231 183 aact:AA02426|5end_uridylyltransferase_1671663..1671893_POSITIVE 322 288 243 85 29 aact:AA00185|5end_3-dehydroquinate_dehydratase_II_130792..131022_POSITIVE 318 100 53 106 48 aact:AA01767|5end_conserved_hypothetical_protein_1192528..1192758_POSITIVE 316 80 38 89 48 aact:AA02076|5end_conserved_hypothetical_protein_1409926..1410156_POSITIVE 316 96 52 77 26 aact:AA01565|5end_conserved_hypothetical_protein_1050711..1050941_POSITIVE 313 80 35 198 141 aact:AA01914|5end_conserved_hypothetical_protein_1290126..1290356_POSITIVE 313 100 51 226 167 aact:AA01721|5end_hypothetical_protein_1163312..1163542_POSITIVE 313 100 51 192 133 aact:AA01720|5end_conserved_hypothetical_protein_1163278..1163508_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 405 225 190 149 114 aact:AA00500|3end_hypothetical_protein_347356..347506_POSITIVE 405 225 190 72 37 aact:AA00499|3end_pyridoxamine-5'-phosphate_oxidase_347279..347429_POSITIVE 347 99 54 125 69 aact:AA00475|3end_conserved_hypothetical_protein_(possible_N6-adenine-specific_methylase)_332282..332432_POSITIVE 343 99 54 66 16 aact:AA00162|3end_pilin_C_biogenesis_protein/protein_transport_protein_116086..116236_POSITIVE 337 120 76 133 93 aact:AA01889|3end_conserved_hypothetical_protein_(possible_permease)_1274590..1274740_POSITIVE 330 250 208 49 7 aact:AA00606|3end_membrane-associated_zinc_metalloprotease_413219..413369_POSITIVE 324 259 212 149 101 aact:AA02425|3end_glutamate-1-semialdehyde_aminotransferase_1671661..1671811_POSITIVE 322 288 243 124 68 aact:AA00184|3end_hypothetical_protein_130911..131061_POSITIVE 321 97 51 62 11 aact:AA01846|3end_conserved_hypothetical_protein_1242165..1242315_POSITIVE 318 100 53 134 76 aact:AA01765|3end_Mu-like_prophage_FluMu_transposase_A_1192636..1192786_POSITIVE 316 80 38 62 21 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE 313 100 51 150 91 aact:AA01718|3end_DNA_polymerase_III,_chi_subunit_1163316..1163466_POSITIVE 313 100 54 144 98 aact:AA01262|3end_hydrolase_860087..860237_POSITIVE 312 185 141 103 62 aact:AA00615|3end_cation_transport_protein_422042..422192_POSITIVE 308 65 27 133 103 aact:AA01818|3end_high-affinity_zinc_uptake_system_membrane_protein_1225078..1225228_POSITIVE 306 96 51 149 104 aact:AA01994|3end_chorismate_mutase;_prephenate_dehydrogenase_1355251..1355401_POSITIVE 306 260 216 52 10 aact:AA01276|3end_heptosyltransferase_III_865844..865994_POSITIVE 303 230 186 43 8 aact:AA00635|3end_dihydropteroate_synthase_436828..436978_POSITIVE 302 118 72 80 34 aact:AA00217|3end_hypothetical_protein_150825..150975_POSITIVE 300 228 196 64 31 aact:AA01893|3end_acetyl-coenzyme-A_carboxylase_subunit_A_1277872..1278022_POSITIVE