Origin IGS: taatttgctttattaaagtgcggttatttttcaagtgtttttattaaacaaatttaaaacacattaaaaattcaccgcacttttattcactcctacgaccaatttaaactgataattatttgcatttcggaaattagacttttagaataaaaatattcatgcaattttaatatgttgcagaataaaccatcgtataaagaacatttaaaaggagttttcaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttgaaaactccttttaaatgttctttatacgatggtttattctgcaacatattaaaattgcatgaatatttttattctaaaagtctaatttccgaaatgcaaataattatcagtttaaattggtcgtaggagtgaataaaagtgcggtgaatttttaatgtgttttaaatttgtttaataaaaacacttgaaaaataaccgcactttaataaagcaaatta Mask Tandem Repeat Region ================================================ taatttgctttattaaagtgcggttatttttcaagtgtttttattaaacaaatttaaaacacattaaaaattcaccgcacttttattcactcctacgaccaatttaaactgataattatttgcatttcggaaattagacttttagaataaaaatattcatgcaattttaatatgttgcagaataaaccatcgtataaagaacatttaaaaggagttttcaa Find is-nt database================================================ Query_seq: AA01046:AA01048|AA01046:AA01048:conserved hypothetical protein:iron binding protein afuA, periplasmic protein:->->:729341..729561 221 taatttgctttattaaagtgcggttatttttcaagtgtttttattaaacaaatttaaaacacattaaaaattcaccgcacttttattcactcctacgaccaatttaaactgataattatttgcatttcggaaattagacttttagaataaaaatattcatgcaattttaatatgttgcagaataaaccatcgtataaagaacatttaaaaggagttttcaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01046:AA01048|AA01046:AA01048:conserved hypothetical protein:iron binding protein afuA, periplasmic protein:->->:729341..729561 221 taatttgctttattaaagtgcggttatttttcaagtgtttttattaaacaaatttaaaacacattaaaaattcaccgcacttttattcactcctacgaccaatttaaactgataattatttgcatttcggaaattagacttttagaataaaaatattcatgcaattttaatatgttgcagaataaaccatcgtataaagaacatttaaaaggagttttcaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01046:AA01048|AA01046:AA01048:conserved hypothetical protein:iron binding protein afuA, periplasmic protein:->->:729341..729561 221 taatttgctttattaaagtgcggttatttttcaagtgtttttattaaacaaatttaaaacacattaaaaattcaccgcacttttattcactcctacgaccaatttaaactgataattatttgcatttcggaaattagacttttagaataaaaatattcatgcaattttaatatgttgcagaataaaccatcgtataaagaacatttaaaaggagttttcaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatttgctttattaaagtgcggttatttttcaagtgtttttattaaacaaatttaaaacacattaaaaattcaccgcacttttattcactcctacgaccaatttaaactgataattatttgcatttcggaaattagacttttagaataaaaatattcatgcaattttaatatgttgcagaataaaccatcgtataaagaacatttaaaaggagttttcaa Predict ORF larger than 30AA ================================================ Protein_Len: 42 Strand: - Start: 96 End: 221 ............................................................................................... S W N L S I I I Q M E S I L S K S Y F Y E H L K L I N C F L G D Y L S C K F P T K M taatttgctttattaaagtgcggttatttttcaagtgtttttattaaacaaatttaaaacacattaaaaattcaccgcacttttattcactcctacgaccaatttaaactgataattatttgcatttcggaaattagacttttagaataaaaatattcatgcaattttaatatgttgcagaataaaccatcgtataaagaacatttaaaaggagttttcaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatttgctttattaaagtgcggttatttttcaagtgtttttattaaacaaatttaaaacacattaaaaattcaccgcacttttattcactcctacgaccaatttaaactgataattatttgcatttcggaaattagacttttagaataaaaatattcatgcaattttaatatgttgcagaataaaccatcgtataaagaacatttaaaaggagttttcaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01046:AA01048|AA01046:AA01048:conserved hypothetical protein:iron binding protein afuA, periplasmic protein:->->:729341..729561 221 taatttgctttattaaagtgcggttatttttcaagtgtttttattaaacaaatttaaaacacattaaaaattcaccgcacttttattcactcctacgaccaatttaaactgataattatttgcatttcggaaattagacttttagaataaaaatattcatgcaattttaatatgttgcagaataaaccatcgtataaagaacatttaaaaggagttttcaa Intra-Species Hit: Count: 1 Min: 1 Max: 221 Len: 221 Subject: aact_AA01046_AA01048|conserved hypothetical protein:iron binding protein afuA, periplasmic protein|POSITIVE:POSITIVE|[729341,729561]|221 HSP 1 e-value: 1.0E-122 bit: 438.0 Len: 221 Query Start:1 Query End:221 Subject Strand: POSITIVE Subject Start: 1 Subject End: 221 taatttgctttattaaagtgcggttatttttcaagtgtttttattaaacaaatttaaaacacattaaaaattcaccgcacttttattcactcctacgaccaatttaaactgataattatttgcatttcggaaattagacttttagaataaaaatattcatgcaattttaatatgttgcagaataaaccatcgtataaagaacatttaaaaggagttttcaa taatttgctttattaaagtgcggttatttttcaagtgtttttattaaacaaatttaaaacacattaaaaattcaccgcacttttattcactcctacgaccaatttaaactgataattatttgcatttcggaaattagacttttagaataaaaatattcatgcaattttaatatgttgcagaataaaccatcgtataaagaacatttaaaaggagttttcaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUUGCUUUAUUAAAGUGCGGUUAUUUUUCAAGUGUUUUUAUUAAACAAAUUUAAAACACAUUAAAAAUUCACCGCACUUUUAUUCACUCCUACGACCAAUUUAAACUGAUAAUUAUUUGCAUUUCGGAAAUUAGACUUUUAGAAUAAAAAUAUUCAUGCAAUUUUAAUAUGUUGCAGAAUAAACCAUCGUAUAAAGAACAUUUAAAAGGAGUUUUCAA .....(((......((((((((((.((((((...(((((((.(((.....)))..)))))))...))))))..))))))))))...((........)).......................)))....(((((((...((((((((.......(((((.((((((........))))))))))).....((.......))...)))))))).))))))).. (-42.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGAAAACUCCUUUUAAAUGUUCUUUAUACGAUGGUUUAUUCUGCAACAUAUUAAAAUUGCAUGAAUAUUUUUAUUCUAAAAGUCUAAUUUCCGAAAUGCAAAUAAUUAUCAGUUUAAAUUGGUCGUAGGAGUGAAUAAAAGUGCGGUGAAUUUUUAAUGUGUUUUAAAUUUGUUUAAUAAAAACACUUGAAAAAUAACCGCACUUUAAUAAAGCAAAUUA ......((((((................(((((.(((((..(((......((((...(((((((((((....)))))......((........)).)))))).))))...)))..)))).).))))))))))).....((((((((((...(((((((.(((((((..............))))))))))))))...)))))))))).............. (-45.53) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 427 88 56 221 189 aact:AA00580|5end_conserved_hypothetical_protein_(possible_membrane_protein)_397003..397233_POSITIVE 366 100 56 224 184 aact:AA00515|5end_hypothetical_protein_355730..355960_POSITIVE 366 100 56 103 63 aact:AA00514|5end_hypothetical_protein_355609..355839_POSITIVE 353 42 10 128 95 aact:AA01845|5end_gamma-glutamyltranspeptidase_precursor_1239841..1240071_POSITIVE 348 47 3 178 124 aact:AA02092|5end_glycogen_synthase_1424674..1424904_POSITIVE 333 43 4 101 62 aact:AA00349|5end_hypothetical_protein_233274..233504_POSITIVE 322 42 5 164 125 aact:AA00987|5end_tetracenomycin_polyketide_synthesis_O-methyltransferase_689745..689975_POSITIVE 320 94 56 136 104 aact:AA01532|5end_hypothetical_protein_1028771..1029001_POSITIVE 320 94 56 70 38 aact:AA01531|5end_hypothetical_protein_1028705..1028935_POSITIVE 320 50 5 190 147 aact:AA00914|5end_aminoacyl-histidine_dipeptidase;_Beta-alanyl-histidine_dipeptidase_628038..628268_POSITIVE 318 42 15 184 156 aact:AA00395|5end_conserved_hypothetical_protein_265278..265508_POSITIVE 318 98 56 125 85 aact:AA00160|5end_pilA-like_biogenesis_protein_/_prepilin_peptidase_dependent_proteinD_112836..113066_POSITIVE 316 50 15 213 178 aact:AA01883|5end_recycling_protein;_permease_1269821..1270051_POSITIVE 314 108 61 192 141 aact:AA01981|5end_4-alpha-glucanotransferase_1337202..1337432_POSITIVE 306 103 65 151 112 aact:AA01933|5end_hypothetical_protein_1306060..1306290_POSITIVE 302 102 66 142 105 aact:AA00132|5end_conserved_hypothetical_protein_(possible_regulator_protein,_VirR_family)_94938..95168_POSITIVE 301 49 7 182 140 aact:AA00464|5end_conserved_hypothetical_protein_323912..324142_POSITIVE 301 42 15 83 55 aact:AA00139|5end_antibiotic_maturation_factor_99098..99328_POSITIVE 299 86 56 97 67 aact:AA00233|5end_hypothetical_protein_162339..162569_POSITIVE 298 108 67 45 2 aact:AA01978|5end_hypothetical_protein_1337055..1337285_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 427 88 56 93 61 aact:AA00579|3end_probable_DNA_recombination_protein_396955..397105_POSITIVE 385 45 3 92 49 aact:AA02172|3end_conserved_hypothetical_protein_1486650..1486800_POSITIVE 366 100 56 100 60 aact:AA00513|3end_pseudouridylate_synthase_(pseudouridine_synthase)_355686..355836_POSITIVE 353 42 10 37 4 aact:AA01843|3end_sensor_protein;_probable_two-component_system_sensor_histidine_kinase_1239830..1239980_POSITIVE 348 47 3 91 37 aact:AA02091|3end_hypothetical_protein_1424667..1424817_POSITIVE 333 43 4 83 44 aact:AA00348|3end_50S_ribosomal_protein_L1_233336..233486_POSITIVE 322 42 15 35 7 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 322 42 5 80 41 aact:AA00985|3end_transcriptional_regulator_(LysR_family)_689741..689891_POSITIVE 320 94 56 34 2 aact:AA01530|3end_conserved_hypothetical_protein_1028749..1028899_POSITIVE 320 50 5 104 61 aact:AA00912|3end_short-chain_dehydrogenase_628032..628182_POSITIVE 318 42 15 74 46 aact:AA00391|3end_arsenate_reductase_265248..265398_POSITIVE 318 98 56 144 104 aact:AA00158|3end_ampD_signalling_protein_(N-acetylmuramyl-L-alanine_amidase)_112935..113085_POSITIVE 316 50 15 77 42 aact:AA01881|3end_adenylate_kinase_1269765..1269915_POSITIVE 306 103 65 103 64 aact:AA01932|3end_tRNA_pseudouridine_synthase_B_1306092..1306242_POSITIVE 304 43 3 57 10 aact:AA01107|3end_adenylosuccinate_lyase_763754..763904_POSITIVE 302 102 66 108 71 aact:AA00131|3end_transcriptional_regulator_(MerR_family)_94984..95134_POSITIVE 301 49 7 88 46 aact:AA00463|3end_class_B_acid_phosphatase_323898..324048_POSITIVE 301 42 15 46 18 aact:AA00138|3end_hypothetical_protein_99141..99291_POSITIVE 299 86 56 77 47 aact:AA00231|3end_long-chain-fatty-acid--CoA_ligase_162399..162549_POSITIVE 299 42 7 78 43 aact:AA00221|3end_NADP-specific_glutamate_dehydrogenase_153865..154015_POSITIVE