Origin IGS: aatcatctgtcctcaaaaaacaataattaagcccccggtactatactttacgccgtaagtttgtaagaatagcctcgttttatttaatattatcgaggatatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatatcctcgataatattaaataaaacgaggctattcttacaaacttacggcgtaaagtatagtaccgggggcttaattattgttttttgaggacagatgatt Mask Tandem Repeat Region ================================================ aatcatctgtcctcaaaaaacaataattaagcccccggtactatactttacgccgtaagtttgtaagaatagcctcgttttatttaatattatcgaggatatt Find is-nt database================================================ Query_seq: AA01039:AA01040|AA01039:AA01040:transcriptional regulator (possible murein gene regulator):possible lipoprotein:->->:724313..724415 103 aatcatctgtcctcaaaaaacaataattaagcccccggtactatactttacgccgtaagtttgtaagaatagcctcgttttatttaatattatcgaggatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01039:AA01040|AA01039:AA01040:transcriptional regulator (possible murein gene regulator):possible lipoprotein:->->:724313..724415 103 aatcatctgtcctcaaaaaacaataattaagcccccggtactatactttacgccgtaagtttgtaagaatagcctcgttttatttaatattatcgaggatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01039:AA01040|AA01039:AA01040:transcriptional regulator (possible murein gene regulator):possible lipoprotein:->->:724313..724415 103 aatcatctgtcctcaaaaaacaataattaagcccccggtactatactttacgccgtaagtttgtaagaatagcctcgttttatttaatattatcgaggatatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 aatcatctgtcctcaaaaaacaataattaagcccccggtactatactttacgccgtaagtttgtaagaatagcctcgttttatttaatattatcgaggatatt Predict ORF larger than 30AA ================================================ aatcatctgtcctcaaaaaacaataattaagcccccggtactatactttacgccgtaagtttgtaagaatagcctcgttttatttaatattatcgaggatatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ aatcatctgtcctcaaaaaacaataattaagcccccggtactatactttacgccgtaagtttgtaagaatagcctcgttttatttaatattatcgaggatatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01039:AA01040|AA01039:AA01040:transcriptional regulator (possible murein gene regulator):possible lipoprotein:->->:724313..724415 103 aatcatctgtcctcaaaaaacaataattaagcccccggtactatactttacgccgtaagtttgtaagaatagcctcgttttatttaatattatcgaggatatt Intra-Species Hit: Count: 1 Min: 1 Max: 103 Len: 103 Subject: aact_AA01039_AA01040|transcriptional regulator (possible murein gene regulator):possible lipoprotein|POSITIVE:POSITIVE|[724313,724415]|103 HSP 1 e-value: 4.0E-52 bit: 204.0 Len: 103 Query Start:1 Query End:103 Subject Strand: POSITIVE Subject Start: 1 Subject End: 103 aatcatctgtcctcaaaaaacaataattaagcccccggtactatactttacgccgtaagtttgtaagaatagcctcgttttatttaatattatcgaggatatt aatcatctgtcctcaaaaaacaataattaagcccccggtactatactttacgccgtaagtttgtaagaatagcctcgttttatttaatattatcgaggatatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUCAUCUGUCCUCAAAAAACAAUAAUUAAGCCCCCGGUACUAUACUUUACGCCGUAAGUUUGUAAGAAUAGCCUCGUUUUAUUUAAUAUUAUCGAGGAUAUU .......(((((((.............(((((...((((............))))...)))))...((((((.......)))))).........))))))).. (-15.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUAUCCUCGAUAAUAUUAAAUAAAACGAGGCUAUUCUUACAAACUUACGGCGUAAAGUAUAGUACCGGGGGCUUAAUUAUUGUUUUUUGAGGACAGAUGAUU ....(((((((.((((.(((.(((..((..(((((.(((((...........)).))).)))))..)).....))).))).))))..)))))))......... (-17.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 328 62 26 72 29 aact:AA00929|5end_DNA_gyrase_subunit_B_637797..638027_POSITIVE 294 99 57 227 176 aact:AA02864|5end_glycerol-3-phosphate_transport_protein_2008784..2009014_POSITIVE 294 99 57 110 59 aact:AA02862|5end_hypothetical_protein_2008667..2008897_POSITIVE 289 54 31 164 138 aact:AA01335|5end_primosomal_protein_N'_901993..902223_POSITIVE 287 78 31 82 31 aact:AA01019|5end_conserved_hypothetical_protein_712223..712453_POSITIVE 276 73 33 121 81 aact:AA01747|5end_single-stranded-DNA-specific_exonuclease_1181359..1181589_POSITIVE 272 56 15 45 2 aact:AA02321|5end_conserved_hypothetical_protein_1585163..1585393_POSITIVE 268 78 31 197 145 aact:AA00894|5end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_619879..620109_POSITIVE 255 79 31 63 5 aact:AA01628|5end_thiamin-monophosphate_kinase_1100888..1101118_POSITIVE 253 80 31 101 49 aact:AA02368|5end_conserved_hypothetical_protein_1623544..1623774_POSITIVE 250 78 45 208 176 aact:AA02236|5end_stringent_starvation_protein_A_1521919..1522149_POSITIVE 250 42 2 205 150 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 250 74 31 189 134 aact:AA00579|5end_probable_DNA_recombination_protein_395265..395495_POSITIVE 248 83 41 185 148 aact:AA01355|5end_large_subunit_pseudouridylate_synthase_D_917228..917458_POSITIVE 247 80 31 220 163 aact:AA00424|5end_hypothetical_protein_287123..287353_POSITIVE 244 80 33 91 51 aact:AA00792|5end_conserved_hypothetical_protein_543142..543372_POSITIVE 241 53 33 58 42 aact:AA01432|5end_lysophospholipase_L2_960715..960945_POSITIVE 240 80 36 105 60 aact:AA02824|5end_conserved_hypothetical_protein_1986131..1986361_POSITIVE 239 73 31 61 13 aact:AA02157|5end_TonB-dependent_outer_membrane_receptor_1475251..1475481_POSITIVE 236 100 52 172 120 aact:AA00227|5end_hypothetical_sequence_156407..156637_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 294 99 57 81 30 aact:AA02861|3end_conserved_hypothetical_protein_2008718..2008868_POSITIVE 287 78 31 79 28 aact:AA01018|3end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_712300..712450_POSITIVE 276 73 33 78 38 aact:AA01746|3end_thiol:disulfide_interchange_protein_1181396..1181546_POSITIVE 253 80 31 57 5 aact:AA02367|3end_conserved_hypothetical_protein_1623580..1623730_POSITIVE 248 56 27 59 29 aact:AA02480|3end_excinuclease_ABC_subunit_B_1718442..1718592_POSITIVE 242 78 38 143 105 aact:AA02935|3end_high-affinity_zinc_uptake_system_periplasmic_binding_protein_2055433..2055583_POSITIVE 241 74 50 45 20 aact:AA00578|3end_DNA_topoisomerase_395201..395351_POSITIVE 240 80 36 63 18 aact:AA02823|3end_exodeoxyribonuclease_V_gamma_chain_1986169..1986319_POSITIVE 239 73 31 56 8 aact:AA02156|3end_conserved_hypothetical_protein_1475326..1475476_POSITIVE 236 100 52 136 84 aact:AA00226|3end_hypothetical_protein_156451..156601_POSITIVE 234 80 31 82 26 aact:AA01565|3end_conserved_hypothetical_protein_1050967..1051117_POSITIVE 234 80 32 75 32 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 233 77 31 144 97 aact:AA00894|3end_selenium_transferase_(Cysteinyl-tRNA(Sec)_selenium_transferase)_621350..621500_POSITIVE 232 63 29 147 116 aact:AA02564|3end_transketolase_N-terminal_section_1786783..1786933_POSITIVE 232 76 35 149 106 aact:AA02266|3end_exodeoxyribonuclease_V_beta_chain_1545422..1545572_POSITIVE 231 77 31 78 36 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 231 79 31 54 8 aact:AA00105|3end_conserved_hypothetical_protein_75609..75759_POSITIVE 230 77 33 128 83 aact:AA02970|3end_tyrosine_phenol-lyase_2077391..2077541_POSITIVE 230 80 31 90 44 aact:AA00528|3end_conserved_hypothetical_protein_361703..361853_POSITIVE 226 67 29 111 70 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE