Origin IGS: atttggcgttttttacccgcccggaaagaattgtcaaacgttatcggactaatgtccgaacacttggcattattgccgagcagtttcacctaacagggaaaattctcaatatcttgcggtctaggcggtctctctggcttgcgttggaaaaatatgcccgtaggcaacggaagaagagcgtaatgacagcacggacaatttcttctgcgttgtgggtggaaacactaagatacaactccgcatgttggatcgcaatcaaatctatctgtgcggaggaatggtttttaatacgactttacaagactttcaccgacatggatcagctatttaatcactgaactgcaaagcatacttcgtatattacaaccaaaaacggtttaaaccggattcaaaaagattgagatctacacaatatagaataggactcagtatctagtttaccggcctatccaacttttaggggcaagccacttttaattcaaatcgtattta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaatacgatttgaattaaaagtggcttgcccctaaaagttggataggccggtaaactagatactgagtcctattctatattgtgtagatctcaatctttttgaatccggtttaaaccgtttttggttgtaatatacgaagtatgctttgcagttcagtgattaaatagctgatccatgtcggtgaaagtcttgtaaagtcgtattaaaaaccattcctccgcacagatagatttgattgcgatccaacatgcggagttgtatcttagtgtttccacccacaacgcagaagaaattgtccgtgctgtcattacgctcttcttccgttgcctacgggcatatttttccaacgcaagccagagagaccgcctagaccgcaagatattgagaattttccctgttaggtgaaactgctcggcaataatgccaagtgttcggacattagtccgataacgtttgacaattctttccgggcgggtaaaaaacgccaaat Mask Tandem Repeat Region ================================================ atttggcgttttttacccgcccggaaagaattgtcaaacgttatcggactaatgtccgaacacttggcattattgccgagcagtttcacctaacagggaaaattctcaatatcttgcggtctaggcggtctctctggcttgcgttggaaaaatatgcccgtaggcaacggaagaagagcgtaatgacagcacggacaatttcttctgcgttgtgggtggaaacactaagatacaactccgcatgttggatcgcaatcaaatctatctgtgcggaggaatggtttttaatacgactttacaagactttcaccgacatggatcagctatttaatcactgaactgcaaagcatacttcgtatattacaaccaaaaacggtttaaaccggattcaaaaagattgagatctacacaatatagaataggactcagtatctagtttaccggcctatccaacttttaggggcaagccacttttaattcaaatcgtattta Find is-nt database================================================ Query_seq: AA01021:AA01022|AA01021:AA01022:hypothetical protein:tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase:->->:714337..714828 492 atttggcgttttttacccgcccggaaagaattgtcaaacgttatcggactaatgtccgaacacttggcattattgccgagcagtttcacctaacagggaaaattctcaatatcttgcggtctaggcggtctctctggcttgcgttggaaaaatatgcccgtaggcaacggaagaagagcgtaatgacagcacggacaatttcttctgcgttgtgggtggaaacactaagatacaactccgcatgttggatcgcaatcaaatctatctgtgcggaggaatggtttttaatacgactttacaagactttcaccgacatggatcagctatttaatcactgaactgcaaagcatacttcgtatattacaaccaaaaacggtttaaaccggattcaaaaagattgagatctacacaatatagaataggactcagtatctagtttaccggcctatccaacttttaggggcaagccacttttaattcaaatcgtattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01021:AA01022|AA01021:AA01022:hypothetical protein:tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase:->->:714337..714828 492 atttggcgttttttacccgcccggaaagaattgtcaaacgttatcggactaatgtccgaacacttggcattattgccgagcagtttcacctaacagggaaaattctcaatatcttgcggtctaggcggtctctctggcttgcgttggaaaaatatgcccgtaggcaacggaagaagagcgtaatgacagcacggacaatttcttctgcgttgtgggtggaaacactaagatacaactccgcatgttggatcgcaatcaaatctatctgtgcggaggaatggtttttaatacgactttacaagactttcaccgacatggatcagctatttaatcactgaactgcaaagcatacttcgtatattacaaccaaaaacggtttaaaccggattcaaaaagattgagatctacacaatatagaataggactcagtatctagtttaccggcctatccaacttttaggggcaagccacttttaattcaaatcgtattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01021:AA01022|AA01021:AA01022:hypothetical protein:tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase:->->:714337..714828 492 atttggcgttttttacccgcccggaaagaattgtcaaacgttatcggactaatgtccgaacacttggcattattgccgagcagtttcacctaacagggaaaattctcaatatcttgcggtctaggcggtctctctggcttgcgttggaaaaatatgcccgtaggcaacggaagaagagcgtaatgacagcacggacaatttcttctgcgttgtgggtggaaacactaagatacaactccgcatgttggatcgcaatcaaatctatctgtgcggaggaatggtttttaatacgactttacaagactttcaccgacatggatcagctatttaatcactgaactgcaaagcatacttcgtatattacaaccaaaaacggtttaaaccggattcaaaaagattgagatctacacaatatagaataggactcagtatctagtttaccggcctatccaacttttaggggcaagccacttttaattcaaatcgtattta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atttggcgttttttacccgcccggaaagaattgtcaaacgttatcggactaatgtccgaacacttggcattattgccgagcagtttcacctaacagggaaaattctcaatatcttgcggtctaggcggtctctctggcttgcgttggaaaaatatgcccgtaggcaacggaagaagagcgtaatgacagcacggacaatttcttctgcgttgtgggtggaaacactaagatacaactccgcatgttggatcgcaatcaaatctatctgtgcggaggaatggtttttaatacgactttacaagactttcaccgacatggatcagctatttaatcactgaactgcaaagcatacttcgtatattacaaccaaaaacggtttaaaccggattcaaaaagattgagatctacacaatatagaataggactcagtatctagtttaccggcctatccaacttttaggggcaagccacttttaattcaaatcgtattta Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: + Start: 1 End: 90 M W R F L P A R K E L S N V I G L M S E H L A L L P S S F T .................................................................................................................................................................................................................................................................................................................................................................................................................. Protein_Len: 39 Strand: + Start: 212 End: 328 ................................................................................................................................................................................................................... M G G N T K I Q L R M L D R N Q I Y L C G G M V F N T T L Q D F H R H G S A I .................................................................................................................................................................... Protein_Len: 60 Strand: + Start: 3 End: 335 .. M A F F T R P E R I V K R Y R T N V R T L G I I A E Q F H L T G K I L N I L R S R R S L W L A L E K Y A R R Q R K K S V ............................................................................................................................................................. Protein_Len: 33 Strand: + Start: 335 End: 433 .............................................................................................................................................................................................................................................................................................................................................. M N C K A Y F V Y Y N Q K R F K P D S K R L R S T Q Y R I G L S I ........................................................... Protein_Len: 44 Strand: - Start: 124 End: 255 ........................................................................................................................... A T E R A Q T P F I H G Y A V S S S R L S L V S L K K Q T T P P F V L I C S R M N S R M ............................................................................................................................................................................................................................................. Protein_Len: 60 Strand: - Start: 89 End: 289 ........................................................................................ I K R D L R D R Q S A N S F Y A R L C R F F L T I V A R V I E E A N H T S V S L Y L E A H Q I A I L D I Q A S S H N K M ........................................................................................................................................................................................................... atttggcgttttttacccgcccggaaagaattgtcaaacgttatcggactaatgtccgaacacttggcattattgccgagcagtttcacctaacagggaaaattctcaatatcttgcggtctaggcggtctctctggcttgcgttggaaaaatatgcccgtaggcaacggaagaagagcgtaatgacagcacggacaatttcttctgcgttgtgggtggaaacactaagatacaactccgcatgttggatcgcaatcaaatctatctgtgcggaggaatggtttttaatacgactttacaagactttcaccgacatggatcagctatttaatcactgaactgcaaagcatacttcgtatattacaaccaaaaacggtttaaaccggattcaaaaagattgagatctacacaatatagaataggactcagtatctagtttaccggcctatccaacttttaggggcaagccacttttaattcaaatcgtattta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atttggcgttttttacccgcccggaaagaattgtcaaacgttatcggactaatgtccgaacacttggcattattgccgagcagtttcacctaacagggaaaattctcaatatcttgcggtctaggcggtctctctggcttgcgttggaaaaatatgcccgtaggcaacggaagaagagcgtaatgacagcacggacaatttcttctgcgttgtgggtggaaacactaagatacaactccgcatgttggatcgcaatcaaatctatctgtgcggaggaatggtttttaatacgactttacaagactttcaccgacatggatcagctatttaatcactgaactgcaaagcatacttcgtatattacaaccaaaaacggtttaaaccggattcaaaaagattgagatctacacaatatagaataggactcagtatctagtttaccggcctatccaacttttaggggcaagccacttttaattcaaatcgtattta Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 82 HP_score: -5.8 Tail_Score: -4.94972 Start: 374 End: 385 Full_Region: caatctttttgaatc cggt ttaa accg tttttggttgtaata .....................................................................................................................................................................................................................................................................................................................................................................................cggtttaaaccg........................................................................................................... Find igs database================================================ Query_seq: AA01021:AA01022|AA01021:AA01022:hypothetical protein:tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase:->->:714337..714828 492 atttggcgttttttacccgcccggaaagaattgtcaaacgttatcggactaatgtccgaacacttggcattattgccgagcagtttcacctaacagggaaaattctcaatatcttgcggtctaggcggtctctctggcttgcgttggaaaaatatgcccgtaggcaacggaagaagagcgtaatgacagcacggacaatttcttctgcgttgtgggtggaaacactaagatacaactccgcatgttggatcgcaatcaaatctatctgtgcggaggaatggtttttaatacgactttacaagactttcaccgacatggatcagctatttaatcactgaactgcaaagcatacttcgtatattacaaccaaaaacggtttaaaccggattcaaaaagattgagatctacacaatatagaataggactcagtatctagtttaccggcctatccaacttttaggggcaagccacttttaattcaaatcgtattta Intra-Species Hit: Count: 1 Min: 1 Max: 492 Len: 492 Subject: aact_AA01021_AA01022|hypothetical protein:tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase|POSITIVE:POSITIVE|[714337,714828]|492 HSP 1 e-value: 0.0 bit: 975.0 Len: 492 Query Start:1 Query End:492 Subject Strand: POSITIVE Subject Start: 1 Subject End: 492 atttggcgttttttacccgcccggaaagaattgtcaaacgttatcggactaatgtccgaacacttggcattattgccgagcagtttcacctaacagggaaaattctcaatatcttgcggtctaggcggtctctctggcttgcgttggaaaaatatgcccgtaggcaacggaagaagagcgtaatgacagcacggacaatttcttctgcgttgtgggtggaaacactaagatacaactccgcatgttggatcgcaatcaaatctatctgtgcggaggaatggtttttaatacgactttacaagactttcaccgacatggatcagctatttaatcactgaactgcaaagcatacttcgtatattacaaccaaaaacggtttaaaccggattcaaaaagattgagatctacacaatatagaataggactcagtatctagtttaccggcctatccaacttttaggggcaagccacttttaattcaaatcgtattta atttggcgttttttacccgcccggaaagaattgtcaaacgttatcggactaatgtccgaacacttggcattattgccgagcagtttcacctaacagggaaaattctcaatatcttgcggtctaggcggtctctctggcttgcgttggaaaaatatgcccgtaggcaacggaagaagagcgtaatgacagcacggacaatttcttctgcgttgtgggtggaaacactaagatacaactccgcatgttggatcgcaatcaaatctatctgtgcggaggaatggtttttaatacgactttacaagactttcaccgacatggatcagctatttaatcactgaactgcaaagcatacttcgtatattacaaccaaaaacggtttaaaccggattcaaaaagattgagatctacacaatatagaataggactcagtatctagtttaccggcctatccaacttttaggggcaagccacttttaattcaaatcgtattta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUUUGGCGUUUUUUACCCGCCCGGAAAGAAUUGUCAAACGUUAUCGGACUAAUGUCCGAACACUUGGCAUUAUUGCCGAGCAGUUUCACCUAACAGGGAAAAUUCUCAAUAUCUUGCGGUCUAGGCGGUCUCUCUGGCUUGCGUUGGAAAAAUAUGCCCGUAGGCAACGGAAGAAGAGCGUAAUGACAGCACGGACAAUUUCUUCUGCGUUGUGGGUGGAAACACUAAGAUACAACUCCGCAUGUUGGAUCGCAAUCAAAUCUAUCUGUGCGGAGGAAUGGUUUUUAAUACGACUUUACAAGACUUUCACCGACAUGGAUCAGCUAUUUAAUCACUGAACUGCAAAGCAUACUUCGUAUAUUACAACCAAAAACGGUUUAAACCGGAUUCAAAAAGAUUGAGAUCUACACAAUAUAGAAUAGGACUCAGUAUCUAGUUUACCGGCCUAUCCAACUUUUAGGGGCAAGCCACUUUUAAUUCAAAUCGUAUUUA ...((((..(((((((((((((((((..(((((((....((((((((((....))))))...(((((((....)))))))..........)))).((((....)))).......(((.(((...(((.(((((((((((((((..((.........)))))))))..))).)).))).)))...))).)))..)))))))..))))).)..))))))))))).............((((....(((((((.((..........((((((((((..(((.(((((((((.......))).))))))))).))).)))))))..((..((((.....))))..))...)).........................((((..((((..(((........((((((.((((..(......)..)))).)))))))))..))))))))....))))))).....))))...))))...................... (-133.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAUACGAUUUGAAUUAAAAGUGGCUUGCCCCUAAAAGUUGGAUAGGCCGGUAAACUAGAUACUGAGUCCUAUUCUAUAUUGUGUAGAUCUCAAUCUUUUUGAAUCCGGUUUAAACCGUUUUUGGUUGUAAUAUACGAAGUAUGCUUUGCAGUUCAGUGAUUAAAUAGCUGAUCCAUGUCGGUGAAAGUCUUGUAAAGUCGUAUUAAAAACCAUUCCUCCGCACAGAUAGAUUUGAUUGCGAUCCAACAUGCGGAGUUGUAUCUUAGUGUUUCCACCCACAACGCAGAAGAAAUUGUCCGUGCUGUCAUUACGCUCUUCUUCCGUUGCCUACGGGCAUAUUUUUCCAACGCAAGCCAGAGAGACCGCCUAGACCGCAAGAUAUUGAGAAUUUUCCCUGUUAGGUGAAACUGCUCGGCAAUAAUGCCAAGUGUUCGGACAUUAGUCCGAUAACGUUUGACAAUUCUUUCCGGGCGGGUAAAAAACGCCAAAU ........(((((.........((((((((........((((((...(((.(((....((((((((((......(((((.(((((..((..((((.....))))....(((((.(((((....)))))...........((((((((((((((((((.((((................)))).))))....)))))))).))))))..))))).....))..))))))))))......((((((((.......))).))))).)))))))))).......(((((..((((((...(..((((.......))))..))))))))))))..))).))).......))))))))))))))......(((((..((.(....).....((((((......(((((..((.....(((.((((....)))).)))..((((((....))))))...))..)))))))))))))..)))))...........))))) (-130.79) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 402 77 33 47 3 aact:AA01615|5end_lipoprotein_releasing_system_transmembrane_1091186..1091416_POSITIVE 387 249 201 51 7 aact:AA02131|5end_conserved_hypothetical_protein_(possible_TrpH_protein)_1454239..1454469_POSITIVE 384 160 114 103 59 aact:AA01995|5end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355120..1355350_POSITIVE 383 160 116 203 151 aact:AA02415|5end_hypothetical_protein_1658443..1658673_POSITIVE 366 170 123 63 21 aact:AA02700|5end_succinyl-CoA_synthase,_alpha_subunit_1883256..1883486_POSITIVE 362 145 102 151 96 aact:AA00333|5end_1-phosphofructokinase;_tagatose-6-phosphate_kinase_225543..225773_POSITIVE 348 48 4 66 25 aact:AA01417|5end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_953256..953486_POSITIVE 345 167 123 69 23 aact:AA02067|5end_conserved_hypothetical_protein;_possible_membrane_protein_1405233..1405463_POSITIVE 345 409 361 113 64 aact:AA00226|5end_hypothetical_protein_156276..156506_POSITIVE 344 47 1 230 168 aact:AA00208|5end_D-ribose_ABC_transporter,_permease_protein_/_Galactoside_transport_system_permease_protein_144097..144327_POSITIVE 341 159 112 193 132 aact:AA00056|5end_cytochrome_c-type_biogenesis_protein_43925..44155_POSITIVE 340 119 73 199 140 aact:AA02702|5end_succinyl-CoA_synthetase,_beta_chain_1884139..1884369_POSITIVE 338 180 131 96 46 aact:AA00075|5end_conserved_hypothetical_protein_54963..55193_POSITIVE 337 203 163 226 187 aact:AA00270|5end_malonyl_coA-acyl_carrier_protein_transacylase_189361..189591_POSITIVE 333 170 125 138 83 aact:AA01173|5end_VapD-homolog_803850..804080_POSITIVE 330 144 112 39 6 aact:AA02125|5end_conserved_hypothetical_protein;_possible_universal_stress_protein_1449412..1449642_POSITIVE 329 158 111 66 21 aact:AA01618|5end_lipoprotein_releasing_system_transmembrane_protein_1093071..1093301_POSITIVE 327 66 23 201 150 aact:AA01262|5end_hydrolase_859576..859806_POSITIVE 325 220 175 112 51 aact:AA00765|5end_hypothetical_protein_527906..528136_POSITIVE 324 59 17 153 109 aact:AA01276|5end_heptosyltransferase_III_864730..864960_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 383 160 114 147 110 aact:AA01994|3end_chorismate_mutase;_prephenate_dehydrogenase_1355251..1355401_POSITIVE 362 145 102 145 90 aact:AA00332|3end_PTS_system,_fructose-specific_IIA/FPr_component_(diphosphoryl_transfer_protein)_225617..225767_POSITIVE 353 170 124 132 77 aact:AA00760|3end_conserved_hypothetical_protein_(possible_peptidase)_523815..523965_POSITIVE 348 48 4 66 25 aact:AA01416|3end_2C-methyl-D-erythritol_2,4-cyclodiphosphate_953336..953486_POSITIVE 347 170 121 56 3 aact:AA00740|3end_citrate_lyase_related_biosynthesis_protein_(2-(5'-triphosphoribosyl)-3'-dephosphocoenzyme-A_synthase)_506749..506899_POSITIVE 345 167 123 70 24 aact:AA02066|3end_hypothetical_protein_1405314..1405464_POSITIVE 329 158 111 63 18 aact:AA01616|3end_lipoprotein_releasing_system_ATP-binding_protein_1093148..1093298_POSITIVE 323 170 125 118 73 aact:AA00943|3end_conserved_hypothetical_protein_654542..654692_POSITIVE 318 48 2 136 101 aact:AA01304|3end_penicillin-binding_protein_1A_885580..885730_POSITIVE 316 465 421 74 30 aact:AA01442|3end_phosphatidylserine_synthetase_969193..969343_POSITIVE 313 289 242 122 65 aact:AA01837|3end_transcriptional_regulatory_protein_1236396..1236546_POSITIVE 311 200 155 56 14 aact:AA02934|3end_hypothetical_protein_2054417..2054567_POSITIVE 311 145 108 88 41 aact:AA01064|3end_conserved_hypothetical_protein_(possible_aminomethyltransferase)_738392..738542_POSITIVE 310 194 155 63 16 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 310 170 126 144 96 aact:AA00015|3end_malate_dehydrogenase_8333..8483_POSITIVE 309 180 132 68 24 aact:AA01301|3end_competence_protein_B_882095..882245_POSITIVE 308 218 174 101 52 aact:AA02977|3end_1-acyl-sn-glycerol-3-phosphate_acyltransferase_2081888..2082038_POSITIVE 308 179 134 59 2 aact:AA00549|3end_ferredoxin-type_protein_376521..376671_POSITIVE 306 148 105 132 87 aact:AA02157|3end_TonB-dependent_outer_membrane_receptor_1476305..1476455_POSITIVE 305 195 158 107 65 aact:AA01967|3end_hypothetical_protein_1331734..1331884_POSITIVE