Origin IGS: taattcaactcgggcgaataaaaggcatctgaaaggtgcctttctttttgtctgtttgtgatatggaaatctagcagtcttcacggctagattgttattatccttattttttattagaatattgccccaaacaatgaggttatttcaggaatcaact .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 agttgattcctgaaataacctcattgtttggggcaatattctaataaaaaataaggataataacaatctagccgtgaagactgctagatttccatatcacaaacagacaaaaagaaaggcacctttcagatgccttttattcgcccgagttgaatta Mask Tandem Repeat Region ================================================ taattcaactcgggcgaataaaaggcatctgaaaggtgcctttctttttgtctgtttgtgatatggaaatctagcagtcttcacggctagattgttattatccttattttttattagaatattgccccaaacaatgaggttatttcaggaatcaact Find is-nt database================================================ Query_seq: AA01014:AA01015|AA01014:AA01015:UTP-glucose-1-phosphate uridylyltransferase:met repressor:->->:710494..710650 157 taattcaactcgggcgaataaaaggcatctgaaaggtgcctttctttttgtctgtttgtgatatggaaatctagcagtcttcacggctagattgttattatccttattttttattagaatattgccccaaacaatgaggttatttcaggaatcaact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01014:AA01015|AA01014:AA01015:UTP-glucose-1-phosphate uridylyltransferase:met repressor:->->:710494..710650 157 taattcaactcgggcgaataaaaggcatctgaaaggtgcctttctttttgtctgtttgtgatatggaaatctagcagtcttcacggctagattgttattatccttattttttattagaatattgccccaaacaatgaggttatttcaggaatcaact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01014:AA01015|AA01014:AA01015:UTP-glucose-1-phosphate uridylyltransferase:met repressor:->->:710494..710650 157 taattcaactcgggcgaataaaaggcatctgaaaggtgcctttctttttgtctgtttgtgatatggaaatctagcagtcttcacggctagattgttattatccttattttttattagaatattgccccaaacaatgaggttatttcaggaatcaact Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taattcaactcgggcgaataaaaggcatctgaaaggtgcctttctttttgtctgtttgtgatatggaaatctagcagtcttcacggctagattgttattatccttattttttattagaatattgccccaaacaatgaggttatttcaggaatcaact Predict ORF larger than 30AA ================================================ Protein_Len: 45 Strand: - Start: 1 End: 135 L E V R A F L L C R F P A K R K T Q K H Y P F R A T K V A L N N N D K N K I L I N G W V M ...................... taattcaactcgggcgaataaaaggcatctgaaaggtgcctttctttttgtctgtttgtgatatggaaatctagcagtcttcacggctagattgttattatccttattttttattagaatattgccccaaacaatgaggttatttcaggaatcaact Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: + Score: 86 Start: 91 End: 120 ..........................................................................................ATTGTTATTATCCTTATTTTTTATTAGAAT..................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taattcaactcgggcgaataaaaggcatctgaaaggtgcctttctttttgtctgtttgtgatatggaaatctagcagtcttcacggctagattgttattatccttattttttattagaatattgccccaaacaatgaggttatttcaggaatcaact Predict TransTerm conf > 70================================================ TransTerm Strand: - Conf: 78 HP_score: -6.5 Tail_Score: -4.63218 Start: 24 End: 40 Full_Region: acagacaaaaagaaa ggcacct ttc agatgcc ttttattcgcccgag .......................ggcacctttcagatgcc..................................................................................................................... TransTerm Strand: + Conf: 73 HP_score: -7.3 Tail_Score: -4.06611 Start: 70 End: 91 Full_Region: TTTGTGATATGGAAA TCTAGCAGT CTTC ACGGCTAGA TTGTTATTATCCTTA .....................................................................TCTAGCAGTCTTCACGGCTAGA.................................................................. Find igs database================================================ Query_seq: AA01014:AA01015|AA01014:AA01015:UTP-glucose-1-phosphate uridylyltransferase:met repressor:->->:710494..710650 157 taattcaactcgggcgaataaaaggcatctgaaaggtgcctttctttttgtctgtttgtgatatggaaatctagcagtcttcacggctagattgttattatccttattttttattagaatattgccccaaacaatgaggttatttcaggaatcaact Intra-Species Hit: Count: 1 Min: 1 Max: 157 Len: 157 Subject: aact_AA01014_AA01015|UTP-glucose-1-phosphate uridylyltransferase:met repressor|POSITIVE:POSITIVE|[710494,710650]|157 HSP 1 e-value: 4.0E-84 bit: 311.0 Len: 157 Query Start:1 Query End:157 Subject Strand: POSITIVE Subject Start: 1 Subject End: 157 taattcaactcgggcgaataaaaggcatctgaaaggtgcctttctttttgtctgtttgtgatatggaaatctagcagtcttcacggctagattgttattatccttattttttattagaatattgccccaaacaatgaggttatttcaggaatcaact taattcaactcgggcgaataaaaggcatctgaaaggtgcctttctttttgtctgtttgtgatatggaaatctagcagtcttcacggctagattgttattatccttattttttattagaatattgccccaaacaatgaggttatttcaggaatcaact Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUCAACUCGGGCGAAUAAAAGGCAUCUGAAAGGUGCCUUUCUUUUUGUCUGUUUGUGAUAUGGAAAUCUAGCAGUCUUCACGGCUAGAUUGUUAUUAUCCUUAUUUUUUAUUAGAAUAUUGCCCCAAACAAUGAGGUUAUUUCAGGAAUCAACU ....(((...((((((((..(((((((((.....)))))))))...))))))))....)))......((((((((.((....)).)))))))).......((((((((((.....))))))..(((.((.....)).)))......))))....... (-39.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AGUUGAUUCCUGAAAUAACCUCAUUGUUUGGGGCAAUAUUCUAAUAAAAAAUAAGGAUAAUAACAAUCUAGCCGUGAAGACUGCUAGAUUUCCAUAUCACAAACAGACAAAAAGAAAGGCACCUUUCAGAUGCCUUUUAUUCGCCCGAGUUGAAUUA ...((((((...((((((.....)))))).((((.....................((((.....((((((((.((....)).))))))))....))))...............((((((((.(.....).))))))))....)))).....)))))) (-33.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 350 57 13 220 178 aact:AA02366|5end_hypothetical_protein_1623020..1623250_POSITIVE 336 58 11 178 126 aact:AA01832|5end_formate_dehydrogenase_formation_protein_E_1233609..1233839_POSITIVE 284 61 23 203 167 aact:AA01411|5end_stationary-phase_survival_protein_951000..951230_POSITIVE 284 59 11 230 180 aact:AA00241|5end_formate_acetyltransferase_activating_enzyme_(anaerobic_ribonucleoside-triphosphate_reductase_activating_protein)_171546..171776_POSITIVE 284 59 11 74 24 aact:AA00240|5end_hypothetical_protein_171390..171620_POSITIVE 284 67 26 175 134 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 278 60 12 170 124 aact:AA00529|5end_outer_membrane_protein,_Omp_18/16_361730..361960_POSITIVE 271 89 41 72 25 aact:AA02045|5end_PTS_system,_galactitol-specific_IIB_component_1391956..1392186_POSITIVE 270 98 51 223 169 aact:AA00734|5end_citrate_lyase,_gamma_chain_(acyl_carrier_protein)_502528..502758_POSITIVE 267 66 23 183 137 aact:AA02568|5end_sugar_phosphotransferase_component_II_B_1788379..1788609_POSITIVE 265 39 11 201 171 aact:AA00270|5end_malonyl_coA-acyl_carrier_protein_transacylase_189361..189591_POSITIVE 264 59 12 137 93 aact:AA02899|5end_oligopeptide_ABC_transporter,_ATP-binding_protein_2030063..2030293_POSITIVE 263 42 4 63 12 aact:AA00725|5end_translation_initiation_factor_1_497981..498211_POSITIVE 261 59 15 155 107 aact:AA02235|5end_stringent_starvation_protein_B_1521446..1521676_POSITIVE 261 59 15 50 2 aact:AA02234|5end_hypothetical_protein_1521341..1521571_POSITIVE 261 60 21 171 131 aact:AA01050|5end_iron(III)_ABC_transporter,_permease_protein_730501..730731_POSITIVE 259 49 3 89 43 aact:AA01610|5end_hypothetical_protein_1088811..1089041_POSITIVE 259 52 11 82 38 aact:AA00952|5end_hemY_protein_homolog_657541..657771_POSITIVE 258 58 11 216 157 aact:AA02910|5end_ribonuclease_E_2036137..2036367_POSITIVE 258 46 11 142 108 aact:AA01982|5end_maltodextrin_phosphorylase_1339387..1339617_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 393 54 11 80 37 aact:AA01014|3end_UTP-glucose-1-phosphate_uridylyltransferase_710433..710583_POSITIVE 350 57 13 81 39 aact:AA02365|3end_hflC_protein_1622961..1623111_POSITIVE 336 58 11 98 46 aact:AA01830|3end_2,3-cyclic-nucleotide_2-phosphodiesterase_1233609..1233759_POSITIVE 295 53 12 114 76 aact:AA02727|3end_hypothetical_protein_1909925..1910075_POSITIVE 278 60 12 63 17 aact:AA00528|3end_conserved_hypothetical_protein_361703..361853_POSITIVE 271 89 41 50 3 aact:AA02044|3end_phosphotransferase_system_enzyme_II,_galactitol_specific,_protein_A_1392014..1392164_POSITIVE 270 98 51 128 74 aact:AA00733|3end_hypothetical_protein_502513..502663_POSITIVE 264 59 12 137 93 aact:AA02898|3end_oligopeptide_transport_ATP-binding_protein_2030143..2030293_POSITIVE 261 59 15 151 103 aact:AA02234|3end_hypothetical_protein_1521522..1521672_POSITIVE 261 60 21 87 47 aact:AA01048|3end_iron_binding_protein_afuA,_periplasmic_protein_730497..730647_POSITIVE 258 53 21 82 47 aact:AA02734|3end_leucine-responsive_regulatory_protein_1915692..1915842_POSITIVE 258 56 12 128 93 aact:AA01825|3end_iron(III)_ABC_transporter,_permease_protein_1229493..1229643_POSITIVE 249 59 11 106 58 aact:AA00979|3end_type_B_carboxylesterase;_p-nitrobenzyl_esterase_685054..685204_POSITIVE 245 60 14 92 40 aact:AA02615|3end_conserved_hypothetical_protein_1823701..1823851_POSITIVE 245 64 30 97 51 aact:AA01176|3end_hypothetical_protein_806517..806667_POSITIVE 243 50 2 50 2 aact:AA02468|3end_conserved_hypothetical_protein_1706797..1706947_POSITIVE 243 109 70 93 54 aact:AA02177|3end_conserved_hypothetical_protein_1489432..1489582_POSITIVE 240 54 13 95 61 aact:AA02103|3end_D-alanyl-D-alanine-endopeptidase;_penicillin-binding_protein_7_1431784..1431934_POSITIVE 238 54 12 132 86 aact:AA01552|3end_hypothetical_protein_1039300..1039450_POSITIVE 238 146 101 94 55 aact:AA01325|3end_formamidopyrimidine-DNA_glycosylase_895639..895789_POSITIVE