Origin IGS: tgtaaatgaaagtttttctcaaaatgtgatccagcacatatttttttttatatcccggtgctactatacaactaaagtaaattaatcctgatcgggtaaggagttgatt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aatcaactccttacccgatcaggattaatttactttagttgtatagtagcaccgggatataaaaaaaaatatgtgctggatcacattttgagaaaaactttcatttaca Mask Tandem Repeat Region ================================================ tgtaaatgaaagtttttctcaaaatgtgatccagcacatatttttttttatatcccggtgctactatacaactaaagtaaattaatcctgatcgggtaaggagttgatt Find is-nt database================================================ Query_seq: AA01008:AA01009|AA01008:AA01009:mazG beta-lactamase regulatory protein homolog:universal stress protein A:->->:704474..704582 109 tgtaaatgaaagtttttctcaaaatgtgatccagcacatatttttttttatatcccggtgctactatacaactaaagtaaattaatcctgatcgggtaaggagttgatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01008:AA01009|AA01008:AA01009:mazG beta-lactamase regulatory protein homolog:universal stress protein A:->->:704474..704582 109 tgtaaatgaaagtttttctcaaaatgtgatccagcacatatttttttttatatcccggtgctactatacaactaaagtaaattaatcctgatcgggtaaggagttgatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01008:AA01009|AA01008:AA01009:mazG beta-lactamase regulatory protein homolog:universal stress protein A:->->:704474..704582 109 tgtaaatgaaagtttttctcaaaatgtgatccagcacatatttttttttatatcccggtgctactatacaactaaagtaaattaatcctgatcgggtaaggagttgatt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 tgtaaatgaaagtttttctcaaaatgtgatccagcacatatttttttttatatcccggtgctactatacaactaaagtaaattaatcctgatcgggtaaggagttgatt Predict ORF larger than 30AA ================================================ tgtaaatgaaagtttttctcaaaatgtgatccagcacatatttttttttatatcccggtgctactatacaactaaagtaaattaatcctgatcgggtaaggagttgatt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ tgtaaatgaaagtttttctcaaaatgtgatccagcacatatttttttttatatcccggtgctactatacaactaaagtaaattaatcctgatcgggtaaggagttgatt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01008:AA01009|AA01008:AA01009:mazG beta-lactamase regulatory protein homolog:universal stress protein A:->->:704474..704582 109 tgtaaatgaaagtttttctcaaaatgtgatccagcacatatttttttttatatcccggtgctactatacaactaaagtaaattaatcctgatcgggtaaggagttgatt Intra-Species Hit: Count: 1 Min: 1 Max: 109 Len: 109 Subject: aact_AA01008_AA01009|mazG beta-lactamase regulatory protein homolog:universal stress protein A|POSITIVE:POSITIVE|[704474,704582]|109 HSP 1 e-value: 1.0E-39 bit: 163.0 Len: 109 Query Start:1 Query End:109 Subject Strand: POSITIVE Subject Start: 1 Subject End: 109 tgtaaatgaaagtttttctcaaaatgtgatccagcacatannnnnnnnnatatcccggtgctactatacaactaaagtaaattaatcctgatcgggtaaggagttgatt tgtaaatgaaagtttttctcaaaatgtgatccagcacatatttttttttatatcccggtgctactatacaactaaagtaaattaatcctgatcgggtaaggagttgatt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGUAAAUGAAAGUUUUUCUCAAAAUGUGAUCCAGCACAUAUUUUUUUUUAUAUCCCGGUGCUACUAUACAACUAAAGUAAAUUAAUCCUGAUCGGGUAAGGAGUUGAUU ..........((((...(((...((.(((((.(((((((((........))))....)))))....(((.......)))..........))))).))...)))..)))) (-14.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AAUCAACUCCUUACCCGAUCAGGAUUAAUUUACUUUAGUUGUAUAGUAGCACCGGGAUAUAAAAAAAAAUAUGUGCUGGAUCACAUUUUGAGAAAAACUUUCAUUUACA ....................(.((((((......)))))).)...((((..((((.(((((........))))).)))).........(((((.....))))).)))). (-14.00) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 251 96 54 114 66 aact:AA01654|5end_periplasmic_maltose-binding_protein_precursor_1119896..1120126_POSITIVE 241 98 51 87 40 aact:AA01432|5end_lysophospholipase_L2_960715..960945_POSITIVE 239 96 52 124 85 aact:AA02797|5end_carbohydrate_kinase;_myo-inositol_catabolism_protein_1963883..1964113_POSITIVE 228 98 52 217 169 aact:AA02879|5end_octaprenyl-diphosphate_synthase_2019523..2019753_POSITIVE 225 99 56 204 146 aact:AA00634|5end_DNA_polymerase_IV_(Pol_IV)_435182..435412_POSITIVE 222 95 56 30 3 aact:AA02674|5end_formate_dehydrogenase-related_protein_1859960..1860190_POSITIVE 217 94 53 227 191 aact:AA00325|5end_conserved_hypothetical_protein_221314..221544_POSITIVE 214 60 25 142 112 aact:AA02199|5end_hypothetical_protein_1497627..1497857_POSITIVE 213 75 31 129 78 aact:AA01338|5end_hypothetical_protein_904967..905197_POSITIVE 212 99 51 48 7 aact:AA01499|5end_fructose_1,6-bisphosphatase_protein_1008357..1008587_POSITIVE 207 97 55 166 123 aact:AA01182|5end_3-oxoacyl-(acyl-carrier-protein)_synthase_809210..809440_POSITIVE 205 61 26 207 180 aact:AA02367|5end_conserved_hypothetical_protein_1623174..1623404_POSITIVE 205 61 26 53 26 aact:AA02366|5end_hypothetical_protein_1623020..1623250_POSITIVE 203 95 54 99 50 aact:AA00163|5end_type_IV_prepilin_peptidase;_type_II_secretion_pathway_related_protein_116006..116236_POSITIVE 202 60 12 189 140 aact:AA01666|5end_penicillin-binding_protein_4;_D-alanyl-D-alanine_carboxypeptidase;_D-alanyl-D-alanine-endopeptidase_1128231..1128461_POSITIVE 199 96 53 89 25 aact:AA02708|5end_probable_glyoxalase_II_family_protein_1889926..1890156_POSITIVE 197 63 31 231 202 aact:AA02545|5end_conserved_hypothetical_protein_1773965..1774195_POSITIVE 195 90 54 102 61 aact:AA01444|5end_ISRS011/IS150_related_transposase,_orfB_969159..969389_POSITIVE 195 90 54 102 61 aact:AA01287|5end_transposase_871583..871813_POSITIVE 194 62 26 74 35 aact:AA02980|5end_conserved_hypothetical_protein_2083305..2083535_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 251 96 54 142 94 aact:AA01651|3end_hypothetical_protein_1120004..1120154_POSITIVE 242 65 31 133 102 aact:AA02544|3end_ATP-dependent_RNA_helicase_1773945..1774095_POSITIVE 239 96 52 78 39 aact:AA02796|3end_possible_transcriptional_regulator,_RpiR_family_1963917..1964067_POSITIVE 225 99 56 109 51 aact:AA00633|3end_hypothetical_protein_435167..435317_POSITIVE 219 99 54 126 76 aact:AA02230|3end_sugar_kinase_1517617..1517767_POSITIVE 217 97 51 124 71 aact:AA01441|3end_tRNA/rRNA_methylase_967643..967793_POSITIVE 214 60 25 127 97 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 213 75 31 113 62 aact:AA01337|3end_cell_division_protein_N_905031..905181_POSITIVE 203 95 54 99 50 aact:AA00162|3end_pilin_C_biogenesis_protein/protein_transport_protein_116086..116236_POSITIVE 202 60 12 82 33 aact:AA01663|3end_GTP_cyclohydrolase_I_1128204..1128354_POSITIVE 197 58 18 99 66 aact:AA02697|3end_hydrogenase_3_maturation_protease_1880408..1880558_POSITIVE 195 90 54 56 15 aact:AA01442|3end_phosphatidylserine_synthetase_969193..969343_POSITIVE 191 58 11 74 32 aact:AA02124|3end_anaerobic_regulatory_protein;_transcription_regulator_Fnr_1449370..1449520_POSITIVE 191 58 31 105 84 aact:AA01145|3end_glycyl-tRNA_synthetase_alpha_subunit_784460..784610_POSITIVE 190 60 18 146 103 aact:AA02782|3end_outer_membrane_haemophore_receptor_(tonB_related)_1948828..1948978_POSITIVE 186 71 46 48 20 aact:AA02022|3end_hypothetical_protein_1376754..1376904_POSITIVE 186 59 14 69 26 aact:AA01146|3end_conserved_hypothetical_protein_784770..784920_POSITIVE 186 61 24 37 3 aact:AA00635|3end_dihydropteroate_synthase_436828..436978_POSITIVE 181 63 29 151 119 aact:AA02904|3end_50S_ribosomal_protein_L31_2032633..2032783_POSITIVE 181 72 32 151 98 aact:AA01262|3end_hydrolase_860087..860237_POSITIVE