Origin IGS: ttgttctacttccggcgtgattgtcagtacttgtttacgtagcacgctattcagttgcagcacgcttttcggttcggtttgattggatttgataatttggtggagaataatttgtttaactgtaatgctcatgaaattgtccttaaaaaactctgtctattataaccgcactttttaccggaactaacaaaatatgttaggattagcgcaattatcggttaatat .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 atattaaccgataattgcgctaatcctaacatattttgttagttccggtaaaaagtgcggttataatagacagagttttttaaggacaatttcatgagcattacagttaaacaaattattctccaccaaattatcaaatccaatcaaaccgaaccgaaaagcgtgctgcaactgaatagcgtgctacgtaaacaagtactgacaatcacgccggaagtagaacaa Mask Tandem Repeat Region ================================================ ttgttctacttccggcgtgattgtcagtacttgtttacgtagcacgctattcagttgcagcacgcttttcggttcggtttgattggatttgataatttggtggagaataatttgtttaactgtaatgctcatgaaattgtccttaaaaaactctgtctattataaccgcactttttaccggaactaacaaaatatgttaggattagcgcaattatcggttaatat Find is-nt database================================================ Query_seq: AA01001:AA01003|AA01001:AA01003:conserved hypothetical protein:conserved hypothetical protein:->->:700035..700259 225 ttgttctacttccggcgtgattgtcagtacttgtttacgtagcacgctattcagttgcagcacgcttttcggttcggtttgattggatttgataatttggtggagaataatttgtttaactgtaatgctcatgaaattgtccttaaaaaactctgtctattataaccgcactttttaccggaactaacaaaatatgttaggattagcgcaattatcggttaatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA01001:AA01003|AA01001:AA01003:conserved hypothetical protein:conserved hypothetical protein:->->:700035..700259 225 ttgttctacttccggcgtgattgtcagtacttgtttacgtagcacgctattcagttgcagcacgcttttcggttcggtttgattggatttgataatttggtggagaataatttgtttaactgtaatgctcatgaaattgtccttaaaaaactctgtctattataaccgcactttttaccggaactaacaaaatatgttaggattagcgcaattatcggttaatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA01001:AA01003|AA01001:AA01003:conserved hypothetical protein:conserved hypothetical protein:->->:700035..700259 225 ttgttctacttccggcgtgattgtcagtacttgtttacgtagcacgctattcagttgcagcacgcttttcggttcggtttgattggatttgataatttggtggagaataatttgtttaactgtaatgctcatgaaattgtccttaaaaaactctgtctattataaccgcactttttaccggaactaacaaaatatgttaggattagcgcaattatcggttaatat Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ttgttctacttccggcgtgattgtcagtacttgtttacgtagcacgctattcagttgcagcacgcttttcggttcggtttgattggatttgataatttggtggagaataatttgtttaactgtaatgctcatgaaattgtccttaaaaaactctgtctattataaccgcactttttaccggaactaacaaaatatgttaggattagcgcaattatcggttaatat Predict ORF larger than 30AA ================================================ Protein_Len: 38 Strand: + Start: 49 End: 162 ................................................ M Q L Q H A F R F G L I G F D N L V E N N L F N C N A H E I V L K K L C L L ............................................................... Protein_Len: 34 Strand: + Start: 83 End: 184 .................................................................................. M D L I I W W R I I C L T V M L M K L S L K N S V Y Y N R T F Y R N ......................................... Protein_Len: 44 Strand: - Start: 1 End: 132 Q E V E P T I T L V Q K R L V S N L Q L V S K P E T Q N S K I I Q H L I I Q K V T I S M ............................................................................................. ttgttctacttccggcgtgattgtcagtacttgtttacgtagcacgctattcagttgcagcacgcttttcggttcggtttgattggatttgataatttggtggagaataatttgtttaactgtaatgctcatgaaattgtccttaaaaaactctgtctattataaccgcactttttaccggaactaacaaaatatgttaggattagcgcaattatcggttaatat Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ttgttctacttccggcgtgattgtcagtacttgtttacgtagcacgctattcagttgcagcacgcttttcggttcggtttgattggatttgataatttggtggagaataatttgtttaactgtaatgctcatgaaattgtccttaaaaaactctgtctattataaccgcactttttaccggaactaacaaaatatgttaggattagcgcaattatcggttaatat Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA01001:AA01003|AA01001:AA01003:conserved hypothetical protein:conserved hypothetical protein:->->:700035..700259 225 ttgttctacttccggcgtgattgtcagtacttgtttacgtagcacgctattcagttgcagcacgcttttcggttcggtttgattggatttgataatttggtggagaataatttgtttaactgtaatgctcatgaaattgtccttaaaaaactctgtctattataaccgcactttttaccggaactaacaaaatatgttaggattagcgcaattatcggttaatat Intra-Species Hit: Count: 1 Min: 1 Max: 225 Len: 225 Subject: aact_AA01001_AA01003|conserved hypothetical protein:conserved hypothetical protein|POSITIVE:POSITIVE|[700035,700259]|225 HSP 1 e-value: 1.0E-124 bit: 446.0 Len: 225 Query Start:1 Query End:225 Subject Strand: POSITIVE Subject Start: 1 Subject End: 225 ttgttctacttccggcgtgattgtcagtacttgtttacgtagcacgctattcagttgcagcacgcttttcggttcggtttgattggatttgataatttggtggagaataatttgtttaactgtaatgctcatgaaattgtccttaaaaaactctgtctattataaccgcactttttaccggaactaacaaaatatgttaggattagcgcaattatcggttaatat ttgttctacttccggcgtgattgtcagtacttgtttacgtagcacgctattcagttgcagcacgcttttcggttcggtttgattggatttgataatttggtggagaataatttgtttaactgtaatgctcatgaaattgtccttaaaaaactctgtctattataaccgcactttttaccggaactaacaaaatatgttaggattagcgcaattatcggttaatat Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUGUUCUACUUCCGGCGUGAUUGUCAGUACUUGUUUACGUAGCACGCUAUUCAGUUGCAGCACGCUUUUCGGUUCGGUUUGAUUGGAUUUGAUAAUUUGGUGGAGAAUAAUUUGUUUAACUGUAAUGCUCAUGAAAUUGUCCUUAAAAAACUCUGUCUAUUAUAACCGCACUUUUUACCGGAACUAACAAAAUAUGUUAGGAUUAGCGCAAUUAUCGGUUAAUAU ...........((((((((.......(((......)))....))))).....((((((.((.....(((((((.(((((....(((((..((((((((.(((.((........(.....).......))))).)))))))).............)))))....)))))........)))))))((((((.....)))))).....))))))))..)))....... (-43.62) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAUUAACCGAUAAUUGCGCUAAUCCUAACAUAUUUUGUUAGUUCCGGUAAAAAGUGCGGUUAUAAUAGACAGAGUUUUUUAAGGACAAUUUCAUGAGCAUUACAGUUAAACAAAUUAUUCUCCACCAAAUUAUCAAAUCCAAUCAAACCGAACCGAAAAGCGUGCUGCAACUGAAUAGCGUGCUACGUAAACAAGUACUGACAAUCACGCCGGAAGUAGAACAA ..............(((..(((...((((((.....))))))(((((((.....((.(((((.(((.((((...)))).))).(((.(((((....(((......)))....)))))....)))......................))))).)).....(((((((((........)))))))))........................))))))).)))..))) (-35.20) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 349 47 4 208 160 aact:AA02496|5end_hypothetical_protein_1731960..1732190_POSITIVE 346 78 33 159 110 aact:AA01417|5end_probable_4-diphosphocytidyl-2C-methyl-D-erthyritol_synthase_(MEP_cytidyltransferase)_(MCT)_953256..953486_POSITIVE 341 58 12 74 27 aact:AA02700|5end_succinyl-CoA_synthase,_alpha_subunit_1883256..1883486_POSITIVE 336 77 33 128 76 aact:AA01527|5end_hypothetical_protein_1025704..1025934_POSITIVE 334 88 41 192 140 aact:AA00993|5end_possible_D-tyrosyl-tRNA(Tyr)_deacylase_694488..694718_POSITIVE 331 86 41 231 192 aact:AA02433|5end_hypothetical_protein_1678300..1678530_POSITIVE 325 98 53 53 9 aact:AA02922|5end_gamma-glutamylphosphate_reductase_2046225..2046455_POSITIVE 323 189 141 146 100 aact:AA02987|5end_histidine_triad_protein_homolog_2088767..2088997_POSITIVE 323 89 42 117 54 aact:AA00734|5end_citrate_lyase,_gamma_chain_(acyl_carrier_protein)_502528..502758_POSITIVE 323 190 143 200 153 aact:AA00679|5end_cytoplasmic_axial_filament_(Ribonuclease_G)_463288..463518_POSITIVE 321 59 14 125 81 aact:AA01618|5end_lipoprotein_releasing_system_transmembrane_protein_1093071..1093301_POSITIVE 318 80 31 223 170 aact:AA00540|5end_hypothetical_protein_370050..370280_POSITIVE 312 103 63 217 163 aact:AA01780|5end_ABC-related_molybdenum_transport_system_permease_protein_1201084..1201314_POSITIVE 310 68 30 209 171 aact:AA02043|5end_tagatose_6-phosphate_kinase_1390218..1390448_POSITIVE 309 49 1 154 110 aact:AA01299|5end_competence_protein_C_880946..881176_POSITIVE 308 87 42 85 38 aact:AA00948|5end_probable_ECF-family_RNA_polymerase_sigma_factor_655447..655677_POSITIVE 306 60 13 58 7 aact:AA02252|5end_conserved_hypothetical_protein_1533840..1534070_POSITIVE 306 59 14 196 149 aact:AA00550|5end_ferredoxin-type_protein_376444..376674_POSITIVE 301 86 43 62 16 aact:AA02455|5end_conserved_hypothetical_protein_1694353..1694583_POSITIVE 298 90 44 68 22 aact:AA00272|5end_3-oxoacyl-[acyl-carrier-protein]_synthase_III_190406..190636_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 338 91 52 136 91 aact:AA00391|3end_arsenate_reductase_265248..265398_POSITIVE 324 78 34 137 87 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 323 190 143 80 33 aact:AA00675|3end_glutaredoxin_2_463248..463398_POSITIVE 321 59 14 122 78 aact:AA01616|3end_lipoprotein_releasing_system_ATP-binding_protein_1093148..1093298_POSITIVE 318 80 31 140 87 aact:AA00539|3end_ADP-L-glycero-D-manno-heptose-6-epimerase_370047..370197_POSITIVE 317 87 43 140 94 aact:AA01116|3end_hypothetical_protein_767840..767990_POSITIVE 309 49 1 151 107 aact:AA01298|3end_competence_protein_D_881023..881173_POSITIVE 308 87 42 67 20 aact:AA00947|3end_hypothetical_protein_655509..655659_POSITIVE 301 86 43 62 16 aact:AA02454|3end_ATP-dependent_helicase_1694433..1694583_POSITIVE 300 50 2 45 6 aact:AA01169|3end_hypothetical_protein_802229..802379_POSITIVE 298 90 44 51 5 aact:AA00271|3end_hypothetical_protein_190469..190619_POSITIVE 295 80 31 118 68 aact:AA02037|3end_hypothetical_protein_1387052..1387202_POSITIVE 292 49 13 50 19 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 289 208 161 59 24 aact:AA01873|3end_mannose-6-phosphate_isomerase_1261940..1262090_POSITIVE 288 73 38 80 43 aact:AA01753|3end_conserved_hypothetical_protein_1185902..1186052_POSITIVE 288 50 10 51 16 aact:AA01168|3end_6-phosphogluconolactonase_802018..802168_POSITIVE 288 87 46 63 11 aact:AA00623|3end_hypothetical_protein_430632..430782_POSITIVE 282 86 42 148 106 aact:AA00269|3end_3-oxoacyl-(acyl-carrier-protein)_reductase/short-chain_alcohol_dehydrogenase_189413..189563_POSITIVE 280 78 31 62 15 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 278 49 4 90 32 aact:AA02969|3end_beta_tyrosinase_(tyrosine_phenol-lyase)_2076417..2076567_POSITIVE