Origin IGS: atagagcggaataagcagggtctcgctgaccgcatcctgaatttaaacatcattacctccttgtggaaacgcgcggttaaaccgcgtagagagtcatgtgtggcggacagtacgacgcaagctgccgtcgggaaaagacgaatgttgtcttcttgagaacaggatgattatagatgagaattattcttaattaaaggctgggattaagggggtgaaaaacgtttggaaaaatattcataaatcactgaagtgatttattcaccccttcggggcccttggcataaccaacgttcaaaaaacggaattttttgtgaccgcacttttgggtatgagatgcttatgttgcaagcccaatagcgaaaagtgcggtcatttttacgggtgaatttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaattcacccgtaaaaatgaccgcacttttcgctattgggcttgcaacataagcatctcatacccaaaagtgcggtcacaaaaaattccgttttttgaacgttggttatgccaagggccccgaaggggtgaataaatcacttcagtgatttatgaatatttttccaaacgtttttcacccccttaatcccagcctttaattaagaataattctcatctataatcatcctgttctcaagaagacaacattcgtcttttcccgacggcagcttgcgtcgtactgtccgccacacatgactctctacgcggtttaaccgcgcgtttccacaaggaggtaatgatgtttaaattcaggatgcggtcagcgagaccctgcttattccgctctat Mask Tandem Repeat Region ================================================ atagagcggaataagcagggtctcgctgaccgcatcctgaatttaaacatcattacctccttgtggaaacgcgcggttaaaccgcgtagagagtcatgtgtggcggacagtacgacgcaagctgccgtcgggaaaagacgaatgttgtcttcttgagaacaggatgattatagatgagaattattcttaattaaaggctgggattaagggggtgaaaaacgtttggaaaaatattcataaatcactgaagtgatttattcaccccttcggggcccttggcataaccaacgttcaaaaaacggaattttttgtgaccgcacttttgggtatgagatgcttatgttgcaagcccaatagcgaaaagtgcggtcatttttacgggtgaatttta Find is-nt database================================================ Query_seq: AA00987:AA00988|AA00987:AA00988:tetracenomycin polyketide synthesis O-methyltransferase:conserved hypothetical protein:->->:690647..691037 391 atagagcggaataagcagggtctcgctgaccgcatcctgaatttaaacatcattacctccttgtggaaacgcgcggttaaaccgcgtagagagtcatgtgtggcggacagtacgacgcaagctgccgtcgggaaaagacgaatgttgtcttcttgagaacaggatgattatagatgagaattattcttaattaaaggctgggattaagggggtgaaaaacgtttggaaaaatattcataaatcactgaagtgatttattcaccccttcggggcccttggcataaccaacgttcaaaaaacggaattttttgtgaccgcacttttgggtatgagatgcttatgttgcaagcccaatagcgaaaagtgcggtcatttttacgggtgaatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00987:AA00988|AA00987:AA00988:tetracenomycin polyketide synthesis O-methyltransferase:conserved hypothetical protein:->->:690647..691037 391 atagagcggaataagcagggtctcgctgaccgcatcctgaatttaaacatcattacctccttgtggaaacgcgcggttaaaccgcgtagagagtcatgtgtggcggacagtacgacgcaagctgccgtcgggaaaagacgaatgttgtcttcttgagaacaggatgattatagatgagaattattcttaattaaaggctgggattaagggggtgaaaaacgtttggaaaaatattcataaatcactgaagtgatttattcaccccttcggggcccttggcataaccaacgttcaaaaaacggaattttttgtgaccgcacttttgggtatgagatgcttatgttgcaagcccaatagcgaaaagtgcggtcatttttacgggtgaatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00987:AA00988|AA00987:AA00988:tetracenomycin polyketide synthesis O-methyltransferase:conserved hypothetical protein:->->:690647..691037 391 atagagcggaataagcagggtctcgctgaccgcatcctgaatttaaacatcattacctccttgtggaaacgcgcggttaaaccgcgtagagagtcatgtgtggcggacagtacgacgcaagctgccgtcgggaaaagacgaatgttgtcttcttgagaacaggatgattatagatgagaattattcttaattaaaggctgggattaagggggtgaaaaacgtttggaaaaatattcataaatcactgaagtgatttattcaccccttcggggcccttggcataaccaacgttcaaaaaacggaattttttgtgaccgcacttttgggtatgagatgcttatgttgcaagcccaatagcgaaaagtgcggtcatttttacgggtgaatttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atagagcggaataagcagggtctcgctgaccgcatcctgaatttaaacatcattacctccttgtggaaacgcgcggttaaaccgcgtagagagtcatgtgtggcggacagtacgacgcaagctgccgtcgggaaaagacgaatgttgtcttcttgagaacaggatgattatagatgagaattattcttaattaaaggctgggattaagggggtgaaaaacgtttggaaaaatattcataaatcactgaagtgatttattcaccccttcggggcccttggcataaccaacgttcaaaaaacggaattttttgtgaccgcacttttgggtatgagatgcttatgttgcaagcccaatagcgaaaagtgcggtcatttttacgggtgaatttta Predict ORF larger than 30AA ================================================ Protein_Len: 51 Strand: + Start: 1 End: 153 M E R N K Q G L A D R I L N L N I I T S L W K R A V K P R R E S C V A D S T T Q A A V G K R R M L S S .............................................................................................................................................................................................................................................. Protein_Len: 30 Strand: + Start: 98 End: 187 ................................................................................................. M W R T V R R K L P S G K D E C C L L E N R M I I D E N Y S ............................................................................................................................................................................................................ Protein_Len: 38 Strand: + Start: 241 End: 354 ................................................................................................................................................................................................................................................ M T E V I Y S P L R G P W H N Q R S K N G I F C D R T F G Y E M L M L Q A Q ..................................... Protein_Len: 42 Strand: + Start: 257 End: 382 ................................................................................................................................................................................................................................................................ M H P F G A L G I T N V Q K T E F F V T A L L G M R C L C C K P N S E K C G H F Y G ......... Protein_Len: 35 Strand: - Start: 80 End: 184 ............................................................................... V A Y L T M H P P C Y S A L Q R R S F V F T T K K L V P H N Y I L I M ............................................................................................................................................................................................................... Protein_Len: 47 Strand: - Start: 3 End: 143 .. S R F L C P R A S R M R F K F M M V E K H F R A T L G R L S D H T A S L V V C A A T P F L R M ........................................................................................................................................................................................................................................................ atagagcggaataagcagggtctcgctgaccgcatcctgaatttaaacatcattacctccttgtggaaacgcgcggttaaaccgcgtagagagtcatgtgtggcggacagtacgacgcaagctgccgtcgggaaaagacgaatgttgtcttcttgagaacaggatgattatagatgagaattattcttaattaaaggctgggattaagggggtgaaaaacgtttggaaaaatattcataaatcactgaagtgatttattcaccccttcggggcccttggcataaccaacgttcaaaaaacggaattttttgtgaccgcacttttgggtatgagatgcttatgttgcaagcccaatagcgaaaagtgcggtcatttttacgggtgaatttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ PromScan Matrix: RpoD-17 Strand: - Score: 83 Start: 167 End: 196 ......................................................................................................................................................................CTTTAATTAAGAATAATTCTCATCTATAAT................................................................................................................................................................................................... Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atagagcggaataagcagggtctcgctgaccgcatcctgaatttaaacatcattacctccttgtggaaacgcgcggttaaaccgcgtagagagtcatgtgtggcggacagtacgacgcaagctgccgtcgggaaaagacgaatgttgtcttcttgagaacaggatgattatagatgagaattattcttaattaaaggctgggattaagggggtgaaaaacgtttggaaaaatattcataaatcactgaagtgatttattcaccccttcggggcccttggcataaccaacgttcaaaaaacggaattttttgtgaccgcacttttgggtatgagatgcttatgttgcaagcccaatagcgaaaagtgcggtcatttttacgggtgaatttta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 48 HP_score: -3.3 Tail_Score: -3.35542 Start: 313 End: 371 Full_Region: AACGGAATTTTTTGT GACCGCACTTTTGGGTATGAGATGC TTATGTT GCAAGCCCAATAGCGAAAAGTGCGGTC ATTTTTACGGGTGAA ........................................................................................................................................................................................................................................................................................................................GACCGCACTTTTGGGTATGAGATGCTTATGTTGCAAGCCCAATAGCGAAAAGTGCGGTC.................... Find igs database================================================ Query_seq: AA00987:AA00988|AA00987:AA00988:tetracenomycin polyketide synthesis O-methyltransferase:conserved hypothetical protein:->->:690647..691037 391 atagagcggaataagcagggtctcgctgaccgcatcctgaatttaaacatcattacctccttgtggaaacgcgcggttaaaccgcgtagagagtcatgtgtggcggacagtacgacgcaagctgccgtcgggaaaagacgaatgttgtcttcttgagaacaggatgattatagatgagaattattcttaattaaaggctgggattaagggggtgaaaaacgtttggaaaaatattcataaatcactgaagtgatttattcaccccttcggggcccttggcataaccaacgttcaaaaaacggaattttttgtgaccgcacttttgggtatgagatgcttatgttgcaagcccaatagcgaaaagtgcggtcatttttacgggtgaatttta Intra-Species Hit: Count: 2 Min: 1 Max: 391 Len: 391 Subject: aact_AA00987_AA00988|tetracenomycin polyketide synthesis O-methyltransferase:conserved hypothetical protein|POSITIVE:POSITIVE|[690647,691037]|391 HSP 1 e-value: 0.0 bit: 775.0 Len: 391 Query Start:1 Query End:391 Subject Strand: POSITIVE Subject Start: 1 Subject End: 391 atagagcggaataagcagggtctcgctgaccgcatcctgaatttaaacatcattacctccttgtggaaacgcgcggttaaaccgcgtagagagtcatgtgtggcggacagtacgacgcaagctgccgtcgggaaaagacgaatgttgtcttcttgagaacaggatgattatagatgagaattattcttaattaaaggctgggattaagggggtgaaaaacgtttggaaaaatattcataaatcactgaagtgatttattcaccccttcggggcccttggcataaccaacgttcaaaaaacggaattttttgtgaccgcacttttgggtatgagatgcttatgttgcaagcccaatagcgaaaagtgcggtcatttttacgggtgaatttta atagagcggaataagcagggtctcgctgaccgcatcctgaatttaaacatcattacctccttgtggaaacgcgcggttaaaccgcgtagagagtcatgtgtggcggacagtacgacgcaagctgccgtcgggaaaagacgaatgttgtcttcttgagaacaggatgattatagatgagaattattcttaattaaaggctgggattaagggggtgaaaaacgtttggaaaaatattcataaatcactgaagtgatttattcaccccttcggggcccttggcataaccaacgttcaaaaaacggaattttttgtgaccgcacttttgggtatgagatgcttatgttgcaagcccaatagcgaaaagtgcggtcatttttacgggtgaatttta Subject: aact_AA01675_AA01678|probable multidrug resistance protein; multidrug-efflux transporter:possible transcriptional regulator|POSITIVE:POSITIVE|[1133650,1133958]|309 HSP 1 e-value: 4.0E-43 bit: 176.0 Len: 109 Query Start:215 Query End:323 Subject Strand: NEGATIVE Subject Start: 68 Subject End: 176 ......................................................................................................................................................................................................................aaaaacgtttggaaaaatattcataaatcactgaagtgatttattcaccccttcggggcccttggcataaccaacgttcaaaaaacggaattttttgtgaccgcacttt.................................................................... ......................................................................................................................................................................................................................aaaaacatttgtaaaaatattcataaatcactgaagtgatttattcaccccttcggggccgttggcatagccaacgttcaaaaaacagaattttttgtgaccgcacttt.................................................................... Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAGAGCGGAAUAAGCAGGGUCUCGCUGACCGCAUCCUGAAUUUAAACAUCAUUACCUCCUUGUGGAAACGCGCGGUUAAACCGCGUAGAGAGUCAUGUGUGGCGGACAGUACGACGCAAGCUGCCGUCGGGAAAAGACGAAUGUUGUCUUCUUGAGAACAGGAUGAUUAUAGAUGAGAAUUAUUCUUAAUUAAAGGCUGGGAUUAAGGGGGUGAAAAACGUUUGGAAAAAUAUUCAUAAAUCACUGAAGUGAUUUAUUCACCCCUUCGGGGCCCUUGGCAUAACCAACGUUCAAAAAACGGAAUUUUUUGUGACCGCACUUUUGGGUAUGAGAUGCUUAUGUUGCAAGCCCAAUAGCGAAAAGUGCGGUCAUUUUUACGGGUGAAUUUUA ...(((((........(((((((((((..((.(((((((.......(((.(((.(((((...(((....)))((((.....))))...))).)).))))))(((((...((.....))...))))).((((((..((((((...))))))))))))...)))))))....(((.(((((.....))))).)))..))..)).....(((((((((.....((((....))))....(((((((((....)))))))))))))))))))))))))))(((.....))).))))).......(((((((...((((((((((((((.(.(((.....((((.......))))...))).).))))))))))))))..........))))))). (-125.52) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUUCACCCGUAAAAAUGACCGCACUUUUCGCUAUUGGGCUUGCAACAUAAGCAUCUCAUACCCAAAAGUGCGGUCACAAAAAAUUCCGUUUUUUGAACGUUGGUUAUGCCAAGGGCCCCGAAGGGGUGAAUAAAUCACUUCAGUGAUUUAUGAAUAUUUUUCCAAACGUUUUUCACCCCCUUAAUCCCAGCCUUUAAUUAAGAAUAAUUCUCAUCUAUAAUCAUCCUGUUCUCAAGAAGACAACAUUCGUCUUUUCCCGACGGCAGCUUGCGUCGUACUGUCCGCCACACAUGACUCUCUACGCGGUUUAACCGCGCGUUUCCACAAGGAGGUAAUGAUGUUUAAAUUCAGGAUGCGGUCAGCGAGACCCUGCUUAUUCCGCUCUAU ...................(((((((((((((.(.(((.(((..(((.......))).)))))).).)))))))))))))........(((....(((((((((((....((((..(((....((.((((((((((((((((....)))))))).(((.....))).........)))))))).))...)))..((((......(((..................((..((((..((.(((((((.......)))))))...))..))))..)).(((((..(((.....))).)))))..))).(((((.....))))).........)))).)))).)))))))))))....))).((((..((((......))))....))))..... (-103.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 843 280 231 231 182 aact:AA00096|5end_L-asparaginase_II_precursor_71280..71510_POSITIVE 707 300 254 184 138 aact:AA00054|5end_cytochrome_c_biogenesis_protein_41393..41623_POSITIVE 643 320 271 227 178 aact:AA02220|5end_ABC_transport_system_permease_protein_1511155..1511385_POSITIVE 571 320 271 83 34 aact:AA01953|5end_hypothetical_protein_1320132..1320362_POSITIVE 561 297 251 216 170 aact:AA01936|5end_tyrosyl-tRNA_synthetase_1307043..1307273_POSITIVE 537 292 251 133 92 aact:AA00240|5end_hypothetical_protein_171390..171620_POSITIVE 500 298 251 165 119 aact:AA01079|5end_conserved_hypothetical_protein_747404..747634_POSITIVE 451 326 283 48 4 aact:AA01813|5end_electron_transport_complex_protein_1220949..1221179_POSITIVE 433 149 101 67 4 aact:AA00290|5end_conserved_hypothetical_protein_201272..201502_POSITIVE 429 147 103 221 164 aact:AA01347|5end_conserved_hypothetical_protein_913209..913439_POSITIVE 428 390 341 108 54 aact:AA02577|5end_nuclease_1795267..1795497_POSITIVE 423 360 312 213 166 aact:AA01327|5end_possible_LOS_biosynthesis_enzyme_(D-glycero-D-manno-heptosyl_transferase)_895568..895798_POSITIVE 422 340 291 148 99 aact:AA01554|5end_conserved_hypothetical_protein;_hypothetical_membrane_protein_1042061..1042291_POSITIVE 408 130 82 59 22 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 400 320 271 217 168 aact:AA01502|5end_serine_acetyltransferase_1010014..1010244_POSITIVE 397 260 211 229 176 aact:AA02915|5end_thioredoxin_m_2040652..2040882_POSITIVE 392 326 281 171 127 aact:AA02867|5end_ribonuclease_T_2013118..2013348_POSITIVE 391 140 93 73 24 aact:AA01423|5end_cell_division_protein_X,_ABC_system_permease_956668..956898_POSITIVE 390 130 84 48 2 aact:AA00923|5end_diaminopimelate_decarboxylase_633993..634223_POSITIVE 389 384 341 218 169 aact:AA02288|5end_conserved_hypothetical_protein_1558590..1558820_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 856 290 241 54 5 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 561 297 251 86 40 aact:AA01934|3end_conserved_hypothetical_protein_1306993..1307143_POSITIVE 537 292 251 81 40 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 500 298 251 134 88 aact:AA01078|3end_transcriptional_regulator_(probable_MerR_family)_747453..747603_POSITIVE 459 149 102 62 1 aact:AA00288|3end_conserved_hypothetical_protein_201347..201497_POSITIVE 458 320 271 70 20 aact:AA01880|3end_N-acetylglucosamine_repressor_1268383..1268533_POSITIVE 451 326 283 52 8 aact:AA01811|3end_electron_transport_complex_protein_1221033..1221183_POSITIVE 440 297 260 38 1 aact:AA01167|3end_glucose-6-phosphate_1-dehydrogenase_801067..801217_POSITIVE 419 339 295 48 1 aact:AA01952|3end_ABC_transporter,_substrate_binding_protein_1320155..1320305_POSITIVE 398 324 283 87 46 aact:AA02913|3end_ribosomal_large_subunit_pseudouridine_synthase_2040527..2040677_POSITIVE 393 384 347 83 47 aact:AA00168|3end_possible_sigma_54_modulation_protein_120240..120390_POSITIVE 392 326 281 81 37 aact:AA02866|3end_lactoylglutathione_lyase_2013108..2013258_POSITIVE 391 140 93 61 12 aact:AA01422|3end_cell_division_protein_E,_ABC_system_ATP-binding_protein_956736..956886_POSITIVE 389 384 341 84 35 aact:AA02286|3end_sugar_ABC_transporter,_ATP-binding_protein_1558536..1558686_POSITIVE 387 117 71 115 55 aact:AA01573|3end_peptide_transport_periplasmic_protein_1061454..1061604_POSITIVE 386 384 342 81 33 aact:AA01280|3end_groES_chaperonin_868530..868680_POSITIVE 379 359 312 65 23 aact:AA01640|3end_restriction_enzyme_type_I_helicase_subunit_1110839..1110989_POSITIVE 375 120 71 120 66 aact:AA01769|3end_hypothetical_protein_1193132..1193282_POSITIVE 375 117 71 122 63 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 371 390 341 88 33 aact:AA00482|3end_hydrolase_(haloacid_dehalogenase-like)_(HAD_superfamily)_336484..336634_POSITIVE