Origin IGS: cggattctcctaaaagtgtcatcatcaagaaagtgcggttattttagcgagcgtttttattttgataaatggggttttattggaaaggctattgagtgaaattcaataattaaaatgccaagttttattcctagggtaacaagttaccctaggtgacgcatagttcagcccctttggggttgaattttatcctctcgcccgcttgcggaagagaggatttatctatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taatagataaatcctctcttccgcaagcgggcgagaggataaaattcaaccccaaaggggctgaactatgcgtcacctagggtaacttgttaccctaggaataaaacttggcattttaattattgaatttcactcaatagcctttccaataaaaccccatttatcaaaataaaaacgctcgctaaaataaccgcactttcttgatgatgacacttttaggagaatccg Mask Tandem Repeat Region ================================================ cggattctcctaaaagtgtcatcatcaagaaagtgcggttattttagcgagcgtttttattttgataaatggggttttattggaaaggctattgagtgaaattcaataattaaaatgccaagttttattcctagggtaacaagttaccctaggtgacgcatagttcagcccctttggggttgaattttatcctctcgcccgcttgcggaagagaggatttatctatta Find is-nt database================================================ Query_seq: AA00981:AA00982|AA00981:AA00982:carboxylesterase:aldo-keto reductase:->->:686438..686665 228 cggattctcctaaaagtgtcatcatcaagaaagtgcggttattttagcgagcgtttttattttgataaatggggttttattggaaaggctattgagtgaaattcaataattaaaatgccaagttttattcctagggtaacaagttaccctaggtgacgcatagttcagcccctttggggttgaattttatcctctcgcccgcttgcggaagagaggatttatctatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00981:AA00982|AA00981:AA00982:carboxylesterase:aldo-keto reductase:->->:686438..686665 228 cggattctcctaaaagtgtcatcatcaagaaagtgcggttattttagcgagcgtttttattttgataaatggggttttattggaaaggctattgagtgaaattcaataattaaaatgccaagttttattcctagggtaacaagttaccctaggtgacgcatagttcagcccctttggggttgaattttatcctctcgcccgcttgcggaagagaggatttatctatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00981:AA00982|AA00981:AA00982:carboxylesterase:aldo-keto reductase:->->:686438..686665 228 cggattctcctaaaagtgtcatcatcaagaaagtgcggttattttagcgagcgtttttattttgataaatggggttttattggaaaggctattgagtgaaattcaataattaaaatgccaagttttattcctagggtaacaagttaccctaggtgacgcatagttcagcccctttggggttgaattttatcctctcgcccgcttgcggaagagaggatttatctatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 cggattctcctaaaagtgtcatcatcaagaaagtgcggttattttagcgagcgtttttattttgataaatggggttttattggaaaggctattgagtgaaattcaataattaaaatgccaagttttattcctagggtaacaagttaccctaggtgacgcatagttcagcccctttggggttgaattttatcctctcgcccgcttgcggaagagaggatttatctatta Predict ORF larger than 30AA ================================================ Protein_Len: 31 Strand: + Start: 4 End: 96 ... M L L K V S S S R K C G Y F S E R F Y F D K W G F I G K A I E .................................................................................................................................... cggattctcctaaaagtgtcatcatcaagaaagtgcggttattttagcgagcgtttttattttgataaatggggttttattggaaaggctattgagtgaaattcaataattaaaatgccaagttttattcctagggtaacaagttaccctaggtgacgcatagttcagcccctttggggttgaattttatcctctcgcccgcttgcggaagagaggatttatctatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ cggattctcctaaaagtgtcatcatcaagaaagtgcggttattttagcgagcgtttttattttgataaatggggttttattggaaaggctattgagtgaaattcaataattaaaatgccaagttttattcctagggtaacaagttaccctaggtgacgcatagttcagcccctttggggttgaattttatcctctcgcccgcttgcggaagagaggatttatctatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -11.9 Tail_Score: -4.50976 Start: 164 End: 184 Full_Region: CTAGGTGACGCATAG TTCAGCCCC TTT GGGGTTGAA TTTTATCCTCTCGCC ...................................................................................................................................................................TTCAGCCCCTTTGGGGTTGAA............................................ Find igs database================================================ Query_seq: AA00981:AA00982|AA00981:AA00982:carboxylesterase:aldo-keto reductase:->->:686438..686665 228 cggattctcctaaaagtgtcatcatcaagaaagtgcggttattttagcgagcgtttttattttgataaatggggttttattggaaaggctattgagtgaaattcaataattaaaatgccaagttttattcctagggtaacaagttaccctaggtgacgcatagttcagcccctttggggttgaattttatcctctcgcccgcttgcggaagagaggatttatctatta Intra-Species Hit: Count: 1 Min: 1 Max: 228 Len: 228 Subject: aact_AA00981_AA00982|carboxylesterase:aldo-keto reductase|POSITIVE:POSITIVE|[686438,686665]|228 HSP 1 e-value: 1.0E-126 bit: 452.0 Len: 228 Query Start:1 Query End:228 Subject Strand: POSITIVE Subject Start: 1 Subject End: 228 cggattctcctaaaagtgtcatcatcaagaaagtgcggttattttagcgagcgtttttattttgataaatggggttttattggaaaggctattgagtgaaattcaataattaaaatgccaagttttattcctagggtaacaagttaccctaggtgacgcatagttcagcccctttggggttgaattttatcctctcgcccgcttgcggaagagaggatttatctatta cggattctcctaaaagtgtcatcatcaagaaagtgcggttattttagcgagcgtttttattttgataaatggggttttattggaaaggctattgagtgaaattcaataattaaaatgccaagttttattcctagggtaacaagttaccctaggtgacgcatagttcagcccctttggggttgaattttatcctctcgcccgcttgcggaagagaggatttatctatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGGAUUCUCCUAAAAGUGUCAUCAUCAAGAAAGUGCGGUUAUUUUAGCGAGCGUUUUUAUUUUGAUAAAUGGGGUUUUAUUGGAAAGGCUAUUGAGUGAAAUUCAAUAAUUAAAAUGCCAAGUUUUAUUCCUAGGGUAACAAGUUACCCUAGGUGACGCAUAGUUCAGCCCCUUUGGGGUUGAAUUUUAUCCUCUCGCCCGCUUGCGGAAGAGAGGAUUUAUCUAUUA .((.....)).....(((((...((((((((((.(((.((........)).))).))).)))))))...(((.((((((.(....)...((((((((...))))))))..)))))).))).........((((((((((....)))))))))).)))))..(((((((((((...)))))))))))..((((((((..(((....)))..)))))))).......... (-74.10) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAGAUAAAUCCUCUCUUCCGCAAGCGGGCGAGAGGAUAAAAUUCAACCCCAAAGGGGCUGAACUAUGCGUCACCUAGGGUAACUUGUUACCCUAGGAAUAAAACUUGGCAUUUUAAUUAUUGAAUUUCACUCAAUAGCCUUUCCAAUAAAACCCCAUUUAUCAAAAUAAAAACGCUCGCUAAAAUAACCGCACUUUCUUGAUGAUGACACUUUUAGGAGAAUCCG ..........((((((((.((((....)))).))))))))....((((.((((...)))).))))...((((...((((((((((....))))))))))........(((((.......(((((((.......)))))))...................(((((....))))).......)))))......)))).((((((((...........))))))))..... (-61.80) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 735 179 131 164 116 aact:AA02466|5end_N-acetylneuraminate_lyase_1704963..1705193_POSITIVE 665 178 133 165 120 aact:AA00978|5end_conserved_hypothetical_protein_683399..683629_POSITIVE 443 215 189 230 202 aact:AA00985|5end_transcriptional_regulator_(LysR_family)_688771..689001_POSITIVE 402 179 131 161 107 aact:AA01848|5end_argininosuccinate_synthetase_1242278..1242508_POSITIVE 389 199 153 181 134 aact:AA02111|5end_killing_protein_suppressor_1436877..1437107_POSITIVE 380 200 156 148 108 aact:AA01991|5end_conserved_hypothetical_protein_(possible_nitroreductase)_1352372..1352602_POSITIVE 367 200 155 142 97 aact:AA01792|5end_conserved_hypothetical_protein_1208058..1208288_POSITIVE 367 207 163 142 98 aact:AA00240|5end_hypothetical_protein_171390..171620_POSITIVE 362 59 26 128 95 aact:AA01059|5end_orotate_phosphoribosyltransferase_735052..735282_POSITIVE 360 58 22 172 138 aact:AA02509|5end_chorismate_synthase_1740952..1741182_POSITIVE 359 58 21 107 71 aact:AA02449|5end_hypothetical_protein_1687575..1687805_POSITIVE 356 60 19 208 163 aact:AA01883|5end_recycling_protein;_permease_1269821..1270051_POSITIVE 348 208 164 173 132 aact:AA00200|5end_thymidylate_synthase_138892..139122_POSITIVE 344 218 172 133 76 aact:AA00976|5end_conserved_hypothetical_protein_681206..681436_POSITIVE 344 78 31 148 95 aact:AA00860|5end_phosphoheptose_isomerase_591866..592096_POSITIVE 341 56 21 231 197 aact:AA02450|5end_conserved_hypothetical_protein_1687701..1687931_POSITIVE 329 199 155 89 34 aact:AA01566|5end_conserved_hypothetical_protein_1051811..1052041_POSITIVE 329 199 155 89 34 aact:AA01261|5end_conserved_hypothetical_protein_856443..856673_POSITIVE 327 208 171 68 25 aact:AA01704|5end_tyrosine-specific_transport_protein_1156170..1156400_POSITIVE 323 59 13 87 34 aact:AA00906|5end_sigma-E_factor_regulatory_protein_625110..625340_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 370 194 160 39 2 aact:AA00977|3end_transcriptional_regulator_(LysR_family)_683334..683484_POSITIVE 367 200 155 139 94 aact:AA01790|3end_riboflavin_bifunctional_biosynthesis_protein_1208135..1208285_POSITIVE 367 207 163 90 46 aact:AA00239|3end_hypothetical_protein_171418..171568_POSITIVE 362 59 26 43 10 aact:AA01057|3end_DnaJ-like_protein_djlA_735047..735197_POSITIVE 360 58 22 147 113 aact:AA02507|3end_penicillin-insensitive_murein_endopeptidase_A_1741007..1741157_POSITIVE 356 60 19 72 27 aact:AA01881|3end_adenylate_kinase_1269765..1269915_POSITIVE 342 191 153 150 110 aact:AA02109|3end_2-acylglycerophosphoethanolamine_acyltransferase;_acyl-acyl_carrier_protein_synthetase_1436933..1437083_POSITIVE 330 58 23 35 1 aact:AA02446|3end_conserved_hypothetical_protein_1687583..1687733_POSITIVE 321 68 28 71 31 aact:AA02057|3end_phosphoglycerate_mutase_1401971..1402121_POSITIVE 321 57 12 115 64 aact:AA00555|3end_hypothetical_protein_380442..380592_POSITIVE 319 207 166 47 1 aact:AA01167|3end_glucose-6-phosphate_1-dehydrogenase_801067..801217_POSITIVE 317 60 28 74 42 aact:AA01557|3end_recombinase_A_1045965..1046115_POSITIVE 317 217 179 137 90 aact:AA00199|3end_hypothetical_protein_138744..138894_POSITIVE 312 57 29 81 53 aact:AA00465|3end_hypothetical_protein_324600..324750_POSITIVE 310 61 30 92 61 aact:AA02172|3end_conserved_hypothetical_protein_1486650..1486800_POSITIVE 307 182 141 39 3 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 306 216 171 81 38 aact:AA01572|3end_peptide_ABC_transporter,_permease_protein_1059838..1059988_POSITIVE 305 59 14 84 28 aact:AA02196|3end_oxaloacetate_decarboxylase_beta_chain_1497692..1497842_POSITIVE 303 55 13 151 111 aact:AA00813|3end_hypothetical_protein_559021..559171_POSITIVE 301 49 9 98 51 aact:AA02075|3end_conserved_hypothetical_protein_1409979..1410129_POSITIVE