Origin IGS: ggggtttccttttgaaagtgggggggactctcgcccacttgcgggttatggtagggtgggatttatcccaccattaaacaatcaatcttattatcggtgggataaatcccaccctacgcttcctttgtgaaaaagccta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taggctttttcacaaaggaagcgtagggtgggatttatcccaccgataataagattgattgtttaatggtgggataaatcccaccctaccataacccgcaagtgggcgagagtcccccccactttcaaaaggaaacccc Mask Tandem Repeat Region ================================================ ggggtttccttttgaaagtgggggggactctcgcccacttgcgggttatggtagggtgggatttatcccaccattaaacaatcaatcttattatcggtgggataaatcccaccctacgcttcctttgtgaaaaagccta Find is-nt database================================================ Query_seq: AA00978:AA00979|AA00978:AA00979:conserved hypothetical protein:type B carboxylesterase; p-nitrobenzyl esterase:->->:684646..684784 139 ggggtttccttttgaaagtgggggggactctcgcccacttgcgggttatggtagggtgggatttatcccaccattaaacaatcaatcttattatcggtgggataaatcccaccctacgcttcctttgtgaaaaagccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00978:AA00979|AA00978:AA00979:conserved hypothetical protein:type B carboxylesterase; p-nitrobenzyl esterase:->->:684646..684784 139 ggggtttccttttgaaagtgggggggactctcgcccacttgcgggttatggtagggtgggatttatcccaccattaaacaatcaatcttattatcggtgggataaatcccaccctacgcttcctttgtgaaaaagccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00978:AA00979|AA00978:AA00979:conserved hypothetical protein:type B carboxylesterase; p-nitrobenzyl esterase:->->:684646..684784 139 ggggtttccttttgaaagtgggggggactctcgcccacttgcgggttatggtagggtgggatttatcccaccattaaacaatcaatcttattatcggtgggataaatcccaccctacgcttcctttgtgaaaaagccta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ggggtttccttttgaaagtgggggggactctcgcccacttgcgggttatggtagggtgggatttatcccaccattaaacaatcaatcttattatcggtgggataaatcccaccctacgcttcctttgtgaaaaagccta Predict ORF larger than 30AA ================================================ ggggtttccttttgaaagtgggggggactctcgcccacttgcgggttatggtagggtgggatttatcccaccattaaacaatcaatcttattatcggtgggataaatcccaccctacgcttcctttgtgaaaaagccta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ggggtttccttttgaaagtgggggggactctcgcccacttgcgggttatggtagggtgggatttatcccaccattaaacaatcaatcttattatcggtgggataaatcccaccctacgcttcctttgtgaaaaagccta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00978:AA00979|AA00978:AA00979:conserved hypothetical protein:type B carboxylesterase; p-nitrobenzyl esterase:->->:684646..684784 139 ggggtttccttttgaaagtgggggggactctcgcccacttgcgggttatggtagggtgggatttatcccaccattaaacaatcaatcttattatcggtgggataaatcccaccctacgcttcctttgtgaaaaagccta Intra-Species Hit: Count: 1 Min: 1 Max: 139 Len: 139 Subject: aact_AA00978_AA00979|conserved hypothetical protein:type B carboxylesterase; p-nitrobenzyl esterase|POSITIVE:POSITIVE|[684646,684784]|139 HSP 1 e-value: 6.0E-61 bit: 234.0 Len: 139 Query Start:1 Query End:139 Subject Strand: POSITIVE Subject Start: 1 Subject End: 139 ggggtttccttttgaaagtnnnnnnnactctcgcccacttgcgggttatggtagggtgggatttatcccaccattaaacaatcaatcttattatcggtgggataaatcccaccctacgcttcctttgtgaaaaagccta ggggtttccttttgaaagtgggggggactctcgcccacttgcgggttatggtagggtgggatttatcccaccattaaacaatcaatcttattatcggtgggataaatcccaccctacgcttcctttgtgaaaaagccta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ GGGGUUUCCUUUUGAAAGUGGGGGGGACUCUCGCCCACUUGCGGGUUAUGGUAGGGUGGGAUUUAUCCCACCAUUAAACAAUCAAUCUUAUUAUCGGUGGGAUAAAUCCCACCCUACGCUUCCUUUGUGAAAAAGCCUA .((((((........(((((((.(((...))).)))))))..(((....(((((((((((((((((((((((..(((.............)))..)))))))))))))))))))))).).))).........)))))). (-64.32) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAGGCUUUUUCACAAAGGAAGCGUAGGGUGGGAUUUAUCCCACCGAUAAUAAGAUUGAUUGUUUAAUGGUGGGAUAAAUCCCACCCUACCAUAACCCGCAAGUGGGCGAGAGUCCCCCCCACUUUCAAAAGGAAACCCC ..((..(((((.........(.(((((((((((((((((((((((..(((((......)))))...)))))))))))))))))))))))).......(.(((((((.(........)))))))).)....)))))..)) (-61.10) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 426 60 18 119 70 aact:AA02433|5end_hypothetical_protein_1678300..1678530_POSITIVE 382 129 89 55 4 aact:AA01159|5end_phosphatase_792828..793058_POSITIVE 365 60 17 207 159 aact:AA02667|5end_hypothetical_protein_1856609..1856839_POSITIVE 359 59 13 63 10 aact:AA00865|5end_conserved_hypothetical_protein_599473..599703_POSITIVE 356 72 32 70 5 aact:AA01171|5end_LysA_activator_protein_802811..803041_POSITIVE 352 68 21 231 180 aact:AA02696|5end_formate_hydrogenlyase_maturation_protein_1879476..1879706_POSITIVE 347 59 22 100 57 aact:AA01741|5end_6-pyruvoyl_tetrahydrobiopterin_synthase_1177193..1177423_POSITIVE 342 60 18 50 14 aact:AA01408|5end_conserved_hypothetical_protein_950191..950421_POSITIVE 341 89 43 133 88 aact:AA02463|5end_N-acetylglucosamine-6-phosphate_deacetylase_1702804..1703034_POSITIVE 340 110 63 199 141 aact:AA01825|5end_iron(III)_ABC_transporter,_permease_protein_1228475..1228705_POSITIVE 335 69 21 184 147 aact:AA02949|5end_hypothetical_protein_2061944..2062174_POSITIVE 335 63 23 81 28 aact:AA00229|5end_DNA_polymerase_III,_alpha_subunit_156863..157093_POSITIVE 334 55 12 63 9 aact:AA00853|5end_hypothetical_protein_588678..588908_POSITIVE 333 63 23 231 199 aact:AA01682|5end_molybdate_ABC_transporter_protein_1135568..1135798_POSITIVE 332 59 11 141 90 aact:AA00379|5end_possible_membrane_protein_254882..255112_POSITIVE 327 68 28 122 82 aact:AA01527|5end_hypothetical_protein_1025704..1025934_POSITIVE 325 100 51 215 164 aact:AA01475|5end_conserved_hypothetical_protein_(possible_cytoplasmic_protein;_DNA_binding_protein)_990364..990594_POSITIVE 325 100 51 110 59 aact:AA01474|5end_hypothetical_protein_990259..990489_POSITIVE 324 60 11 200 130 aact:AA00500|5end_hypothetical_protein_347151..347381_POSITIVE 321 59 16 221 173 aact:AA00929|5end_DNA_gyrase_subunit_B_637797..638027_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 426 60 18 64 15 aact:AA02432|3end_penicillin-binding_protein_1B_1678325..1678475_POSITIVE 388 117 96 40 19 aact:AA00978|3end_conserved_hypothetical_protein_684585..684735_POSITIVE 385 69 21 94 42 aact:AA02695|3end_hydrogenase-3_component_G_1879418..1879568_POSITIVE 365 60 17 101 53 aact:AA02666|3end_DNA_helicase_II_1856583..1856733_POSITIVE 356 60 16 132 73 aact:AA00927|3end_ATP-dependent_DNA_helicase_637777..637927_POSITIVE 347 59 22 70 27 aact:AA01740|3end_conserved_hypothetical_protein_1177243..1177393_POSITIVE 335 47 1 72 21 aact:AA02332|3end_conserved_hypothetical_protein;_possible_glycosyltransferase_1594392..1594542_POSITIVE 332 42 3 84 39 aact:AA02420|3end_ISRSO11_related_transposase_1662668..1662818_POSITIVE 326 45 6 69 18 aact:AA01357|3end_5-formyltetrahydrofolate_cyclo-ligase-family_protein_919785..919935_POSITIVE 325 49 1 91 36 aact:AA01675|3end_probable_multidrug_resistance_protein;_multidrug-efflux_transporter_1133589..1133739_POSITIVE 325 59 18 47 3 aact:AA00378|3end_leucine-responsive_regulatory_protein_(transcription_regulator)_254868..255018_POSITIVE 322 57 11 67 13 aact:AA00473|3end_tryptophanyl-tRNA_synthetase_330077..330227_POSITIVE 316 59 14 139 83 aact:AA00954|3end_uroporphyrinogen_III_synthase_661070..661220_POSITIVE 312 57 17 46 2 aact:AA02918|3end_hypothetical_protein_2043501..2043651_POSITIVE 311 70 22 64 3 aact:AA02465|3end_glucosamine-6-phosphate_isomerase_1704885..1705035_POSITIVE 309 60 18 60 19 aact:AA01398|3end_KDO_kinase_945691..945841_POSITIVE 309 68 23 93 49 aact:AA00933|3end_ribose_ABC_transporter,_ATP-binding_protein_645197..645347_POSITIVE 308 42 1 107 59 aact:AA01284|3end_heat_shock_protein_(hsp60)_870294..870444_POSITIVE 299 72 32 150 113 aact:AA02865|3end_iron-regulated_outer_membrane_protein_(omp64)_2012542..2012692_POSITIVE 295 57 14 96 47 aact:AA01288|3end_hypothetical_protein_872288..872438_POSITIVE