Origin IGS: taatcgcaaaaaaacgctttaaaattcgaccgcactttggtttcagcaatcaaagtgcggtttgttttttgggagattttgcaatttatgatcgagaaaataatcgctctttacatatcctcccgcttgcttgcgggagaagggagatgaaagaatta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taattctttcatctcccttctcccgcaagcaagcgggaggatatgtaaagagcgattattttctcgatcataaattgcaaaatctcccaaaaaacaaaccgcactttgattgctgaaaccaaagtgcggtcgaattttaaagcgtttttttgcgatta Mask Tandem Repeat Region ================================================ taatcgcaaaaaaacgctttaaaattcgaccgcactttggtttcagcaatcaaagtgcggtttgttttttgggagattttgcaatttatgatcgagaaaataatcgctctttacatatcctcccgcttgcttgcgggagaagggagatgaaagaatta Find is-nt database================================================ Query_seq: AA00974:AA00976|AA00974:AA00976:glucosamine-fructose-6-phosphate:conserved hypothetical protein:->->:681188..681345 158 taatcgcaaaaaaacgctttaaaattcgaccgcactttggtttcagcaatcaaagtgcggtttgttttttgggagattttgcaatttatgatcgagaaaataatcgctctttacatatcctcccgcttgcttgcgggagaagggagatgaaagaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00974:AA00976|AA00974:AA00976:glucosamine-fructose-6-phosphate:conserved hypothetical protein:->->:681188..681345 158 taatcgcaaaaaaacgctttaaaattcgaccgcactttggtttcagcaatcaaagtgcggtttgttttttgggagattttgcaatttatgatcgagaaaataatcgctctttacatatcctcccgcttgcttgcgggagaagggagatgaaagaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00974:AA00976|AA00974:AA00976:glucosamine-fructose-6-phosphate:conserved hypothetical protein:->->:681188..681345 158 taatcgcaaaaaaacgctttaaaattcgaccgcactttggtttcagcaatcaaagtgcggtttgttttttgggagattttgcaatttatgatcgagaaaataatcgctctttacatatcctcccgcttgcttgcgggagaagggagatgaaagaatta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taatcgcaaaaaaacgctttaaaattcgaccgcactttggtttcagcaatcaaagtgcggtttgttttttgggagattttgcaatttatgatcgagaaaataatcgctctttacatatcctcccgcttgcttgcgggagaagggagatgaaagaatta Predict ORF larger than 30AA ================================================ Protein_Len: 37 Strand: + Start: 37 End: 147 .................................... M V S A I K V R F V F W E I L Q F M I E K I I A L Y I S S R L L A G E G R ........... Protein_Len: 52 Strand: + Start: 3 End: 158 .. M A K K R F K I R P H F G F S N Q S A V C F L G D F A I Y D R E N N R S L H I L P L A C G R R E M K E L Protein_Len: 37 Strand: - Start: 46 End: 156 ............................................. C D F H P K N K P L N Q L K H D L F Y D S K V Y G G A Q K R S F P S S L M .. Protein_Len: 34 Strand: - Start: 3 End: 104 .. D C F F A K F N S R V K T E A I L T R N T K Q S I K C N I I S F I M ...................................................... taatcgcaaaaaaacgctttaaaattcgaccgcactttggtttcagcaatcaaagtgcggtttgttttttgggagattttgcaatttatgatcgagaaaataatcgctctttacatatcctcccgcttgcttgcgggagaagggagatgaaagaatta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taatcgcaaaaaaacgctttaaaattcgaccgcactttggtttcagcaatcaaagtgcggtttgttttttgggagattttgcaatttatgatcgagaaaataatcgctctttacatatcctcccgcttgcttgcgggagaagggagatgaaagaatta Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 100 HP_score: -14.9 Tail_Score: -5.04249 Start: 30 End: 60 Full_Region: CGCTTTAAAATTCGA CCGCACTTTGGTT TCAGC AATCAAAGTGCGG TTTGTTTTTTGGGAG .............................CCGCACTTTGGTTTCAGCAATCAAAGTGCGG.................................................................................................. Find igs database================================================ Query_seq: AA00974:AA00976|AA00974:AA00976:glucosamine-fructose-6-phosphate:conserved hypothetical protein:->->:681188..681345 158 taatcgcaaaaaaacgctttaaaattcgaccgcactttggtttcagcaatcaaagtgcggtttgttttttgggagattttgcaatttatgatcgagaaaataatcgctctttacatatcctcccgcttgcttgcgggagaagggagatgaaagaatta Intra-Species Hit: Count: 1 Min: 15 Max: 158 Len: 144 Subject: aact_AA00974_AA00976|glucosamine-fructose-6-phosphate:conserved hypothetical protein|POSITIVE:POSITIVE|[681188,681345]|158 HSP 1 e-value: 2.0E-76 bit: 285.0 Len: 144 Query Start:15 Query End:158 Subject Strand: POSITIVE Subject Start: 15 Subject End: 158 ..............cgctttaaaattcgaccgcactttggtttcagcaatcaaagtgcggtttgttttttgggagattttgcaatttatgatcgagaaaataatcgctctttacatatcctcccgcttgcttgcgggagaagggagatgaaagaatta ..............cgctttaaaattcgaccgcactttggtttcagcaatcaaagtgcggtttgttttttgggagattttgcaatttatgatcgagaaaataatcgctctttacatatcctcccgcttgcttgcgggagaagggagatgaaagaatta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ CGCUUUAAAAUUCGACCGCACUUUGGUUUCAGCAAUCAAAGUGCGGUUUGUUUUUUGGGAGAUUUUGCAAUUUAUGAUCGAGAAAAUAAUCGCUCUUUACAUAUCCUCCCGCUUGCUUGCGGGAGAAGGGAGAUGAAAGAAUUA ..((..(((((..(((((((((((((((.....))))))))))))))).)))))..))..((((((...((((..((.(((........))).))..........(((((((......))))))).....))))...)))))). (-45.80) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUUCUUUCAUCUCCCUUCUCCCGCAAGCAAGCGGGAGGAUAUGUAAAGAGCGAUUAUUUUCUCGAUCAUAAAUUGCAAAAUCUCCCAAAAAACAAACCGCACUUUGAUUGCUGAAACCAAAGUGCGGUCGAAUUUUAAAGCG ....((((((((.(((....((((((......))))))))).))).)))))((((((...............))))))...................(((((((((((.((.....)).))))))))))).............. (-39.06) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 545 48 1 147 100 aact:AA02252|5end_conserved_hypothetical_protein_1533840..1534070_POSITIVE 536 50 1 148 99 aact:AA00987|5end_tetracenomycin_polyketide_synthesis_O-methyltransferase_689745..689975_POSITIVE 516 54 13 153 111 aact:AA02937|5end_conserved_hypothetical_protein_2056379..2056609_POSITIVE 467 55 14 156 115 aact:AA01692|5end_hexulose-6-phosphate_synthase_1149178..1149408_POSITIVE 463 58 13 177 126 aact:AA02410|5end_cell_division_protein_1655429..1655659_POSITIVE 450 56 13 156 111 aact:AA00792|5end_conserved_hypothetical_protein_543142..543372_POSITIVE 443 49 1 149 100 aact:AA00390|5end_permease_263689..263919_POSITIVE 433 59 13 157 111 aact:AA00613|5end_lipid-A-disaccharide_synthase_418713..418943_POSITIVE 433 56 14 159 119 aact:AA00511|5end_nicotinamide_mononucleotide_transporter_353884..354114_POSITIVE 430 58 13 62 16 aact:AA01853|5end_hypothetical_protein_1246458..1246688_POSITIVE 428 59 13 157 111 aact:AA01603|5end_oligopeptidase_A_1082898..1083128_POSITIVE 428 56 13 165 122 aact:AA00464|5end_conserved_hypothetical_protein_323912..324142_POSITIVE 427 59 14 170 128 aact:AA01519|5end_conserved_hypothetical_protein_1021794..1022024_POSITIVE 423 47 1 148 104 aact:AA02980|5end_conserved_hypothetical_protein_2083305..2083535_POSITIVE 421 133 91 231 193 aact:AA00985|5end_transcriptional_regulator_(LysR_family)_688771..689001_POSITIVE 417 48 1 139 98 aact:AA02137|5end_transcriptional_regulators_(TetR/AcrR_family)_1460453..1460683_POSITIVE 416 57 12 205 158 aact:AA01524|5end_amino_acid_ABC_transporter,_ATP-binding_protein_1023500..1023730_POSITIVE 414 59 14 84 28 aact:AA02366|5end_hypothetical_protein_1623020..1623250_POSITIVE 407 49 1 140 98 aact:AA02457|5end_conserved_hypothetical_protein_1695320..1695550_POSITIVE 402 59 14 163 123 aact:AA02963|5end_opacity_associated_protein_B_2072302..2072532_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 545 48 1 70 23 aact:AA02251|3end_biotin_sulfoxide_reductase;_Trimethylamine-N-oxide_reductase_precursor_1533843..1533993_POSITIVE 536 50 1 64 15 aact:AA00985|3end_transcriptional_regulator_(LysR_family)_689741..689891_POSITIVE 516 54 13 67 25 aact:AA02936|3end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2056373..2056523_POSITIVE 463 58 13 72 21 aact:AA02409|3end_DNA_ligase_1655404..1655554_POSITIVE 450 56 13 68 23 aact:AA00790|3end_recombination_DNA_repair_protein_543134..543284_POSITIVE 433 59 13 69 23 aact:AA00612|3end_acyl-(acyl-carrier-protein)-UDP-N-acetylglucosami_ne_418705..418855_POSITIVE 433 56 14 78 38 aact:AA00508|3end_SAM-dependent_methyltransferase_353883..354033_POSITIVE 430 58 13 75 29 aact:AA01852|3end_peptide_chain_release_factor_3_1246551..1246701_POSITIVE 428 56 13 71 28 aact:AA00463|3end_class_B_acid_phosphatase_323898..324048_POSITIVE 427 59 14 87 45 aact:AA01517|3end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1021791..1021941_POSITIVE 425 55 16 40 1 aact:AA01690|3end_penicillin-binding_protein_1C_1149142..1149292_POSITIVE 423 47 1 71 27 aact:AA02978|3end_sufI_protein_precursor_homolog_2083308..2083458_POSITIVE 417 48 1 82 41 aact:AA02136|3end_aspartate_aminotransferase_1460476..1460626_POSITIVE 412 47 13 62 27 aact:AA00974|3end_glucosamine-fructose-6-phosphate_681127..681277_POSITIVE 402 59 14 66 26 aact:AA02962|3end_recombination_associated_protein_2072285..2072435_POSITIVE 402 59 14 64 17 aact:AA02758|3end_3-phosphoshikimate_1-carboxyvinyltransferase_1933150..1933300_POSITIVE 402 60 11 83 30 aact:AA00183|3end_acetyl-CoA_carboxylase,_biotin_carboxyl_carrier_protein_130745..130895_POSITIVE 400 47 1 48 12 aact:AA00506|3end_ATP-dependent_RNA_helicase_protein_353089..353239_POSITIVE 396 47 7 58 21 aact:AA00072|3end_conserved_hypothetical_protein_54947..55097_POSITIVE 395 58 12 111 64 aact:AA02135|3end_hypothetical_protein_1459067..1459217_POSITIVE