Origin IGS: taaaattttatcgaaaaacaaggccgcctgaataaaaaaattgactttttattgaaaagtgcgatcacaaaaaacaacgtttttttaaatcgggtgaataaaaatttatgtatttctcacgcagttaaacaggtgctaaaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttttagcacctgtttaactgcgtgagaaatacataaatttttattcacccgatttaaaaaaacgttgttttttgtgatcgcacttttcaataaaaagtcaatttttttattcaggcggccttgtttttcgataaaatttta Mask Tandem Repeat Region ================================================ taaaattttatcgaaaaacaaggccgcctgaataaaaaaattgactttttattgaaaagtgcgatcacaaaaaacaacgtttttttaaatcgggtgaataaaaatttatgtatttctcacgcagttaaacaggtgctaaaa Find is-nt database================================================ Query_seq: AA00967:AA00969|AA00967:AA00969:conserved hypothetical protein:conserved hypothetical protein (possible NADH pyrophosphatase):->->:675328..675468 141 taaaattttatcgaaaaacaaggccgcctgaataaaaaaattgactttttattgaaaagtgcgatcacaaaaaacaacgtttttttaaatcgggtgaataaaaatttatgtatttctcacgcagttaaacaggtgctaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00967:AA00969|AA00967:AA00969:conserved hypothetical protein:conserved hypothetical protein (possible NADH pyrophosphatase):->->:675328..675468 141 taaaattttatcgaaaaacaaggccgcctgaataaaaaaattgactttttattgaaaagtgcgatcacaaaaaacaacgtttttttaaatcgggtgaataaaaatttatgtatttctcacgcagttaaacaggtgctaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00967:AA00969|AA00967:AA00969:conserved hypothetical protein:conserved hypothetical protein (possible NADH pyrophosphatase):->->:675328..675468 141 taaaattttatcgaaaaacaaggccgcctgaataaaaaaattgactttttattgaaaagtgcgatcacaaaaaacaacgtttttttaaatcgggtgaataaaaatttatgtatttctcacgcagttaaacaggtgctaaaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taaaattttatcgaaaaacaaggccgcctgaataaaaaaattgactttttattgaaaagtgcgatcacaaaaaacaacgtttttttaaatcgggtgaataaaaatttatgtatttctcacgcagttaaacaggtgctaaaa Predict ORF larger than 30AA ================================================ Protein_Len: 44 Strand: + Start: 10 End: 141 ......... M E K Q G R L N K K I D F L L K S A I T K N N V F L N R V N K N L C I S H A V K Q V L K Protein_Len: 37 Strand: - Start: 3 End: 113 .. F K I S F C P R R F L F I S K K N F L A I V F F L T K K F R T F L F K H M ............................ taaaattttatcgaaaaacaaggccgcctgaataaaaaaattgactttttattgaaaagtgcgatcacaaaaaacaacgtttttttaaatcgggtgaataaaaatttatgtatttctcacgcagttaaacaggtgctaaaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taaaattttatcgaaaaacaaggccgcctgaataaaaaaattgactttttattgaaaagtgcgatcacaaaaaacaacgtttttttaaatcgggtgaataaaaatttatgtatttctcacgcagttaaacaggtgctaaaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00967:AA00969|AA00967:AA00969:conserved hypothetical protein:conserved hypothetical protein (possible NADH pyrophosphatase):->->:675328..675468 141 taaaattttatcgaaaaacaaggccgcctgaataaaaaaattgactttttattgaaaagtgcgatcacaaaaaacaacgtttttttaaatcgggtgaataaaaatttatgtatttctcacgcagttaaacaggtgctaaaa Intra-Species Hit: Count: 1 Min: 1 Max: 141 Len: 141 Subject: aact_AA00967_AA00969|conserved hypothetical protein:conserved hypothetical protein (possible NADH pyrophosphatase)|POSITIVE:POSITIVE|[675328,675468]|141 HSP 1 e-value: 1.0E-49 bit: 196.0 Len: 141 Query Start:1 Query End:141 Subject Strand: POSITIVE Subject Start: 1 Subject End: 141 taaaattttatcgaaaaacaaggccgcctgaatnnnnnnnttgactttttattgaaaagtgcgatcacaaaaaacaacgnnnnnnnaaatcgggtgaataaaaatttatgtatttctcacgcagttaaacaggtgctaaaa taaaattttatcgaaaaacaaggccgcctgaataaaaaaattgactttttattgaaaagtgcgatcacaaaaaacaacgtttttttaaatcgggtgaataaaaatttatgtatttctcacgcagttaaacaggtgctaaaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAUUUUAUCGAAAAACAAGGCCGCCUGAAUAAAAAAAUUGACUUUUUAUUGAAAAGUGCGAUCACAAAAAACAACGUUUUUUUAAAUCGGGUGAAUAAAAAUUUAUGUAUUUCUCACGCAGUUAAACAGGUGCUAAAA .....................(((.(((((((((((((.......))))))))...((.((((.....(((((((...)))))))......(.((((((....)))))).)........)))).))...)))))))).... (-20.30) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUUUAGCACCUGUUUAACUGCGUGAGAAAUACAUAAAUUUUUAUUCACCCGAUUUAAAAAAACGUUGUUUUUUGUGAUCGCACUUUUCAAUAAAAAGUCAAUUUUUUUAUUCAGGCGGCCUUGUUUUUCGAUAAAAUUUUA .....((.((((..(((....(((((((((......)))))...)))).(((((..(((((((...)))))))..))))).((((((.....))))))........)))..))))..))...................... (-17.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 331 85 54 227 193 aact:AA02168|5end_possible_protease;_heat_shock_protein_1483529..1483759_POSITIVE 296 97 53 177 134 aact:AA00119|5end_ATP-dependent_Clp_protease_proteolytic_subunit_(endopeptidase_Clp)_88387..88617_POSITIVE 290 93 56 165 126 aact:AA01090|5end_leucyl-tRNA_synthetase,_leucyl-tRNA_ligase_753548..753778_POSITIVE 284 92 51 141 99 aact:AA01648|5end_alpha-amylase_protein;_1,4-alpha-D-glucan_glucanohydrolase_1115170..1115400_POSITIVE 282 139 91 145 100 aact:AA01313|5end_deoxyuridinetriphosphatase;_deoxyuridine_5'-triphosphate_nucleotidohydrolase_889567..889797_POSITIVE 274 91 55 182 146 aact:AA02050|5end_1-deoxy-D-xylulose_5-phosphate_synthase_1395625..1395855_POSITIVE 274 91 55 83 47 aact:AA02049|5end_hypothetical_protein_1395526..1395756_POSITIVE 274 137 92 120 67 aact:AA01679|5end_aspartate-semialdehyde_dehydrogenase;_PTS_system_enzyme_II_A_component_1134356..1134586_POSITIVE 273 95 55 199 159 aact:AA00988|5end_conserved_hypothetical_protein_690898..691128_POSITIVE 266 63 24 144 98 aact:AA02762|5end_ribonuclease_H_1933164..1933394_POSITIVE 263 99 59 194 146 aact:AA02699|5end_formate_dehydrogenase_H,_selenopolypeptide_subunit_1881397..1881627_POSITIVE 262 92 54 188 150 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 260 67 21 163 116 aact:AA01151|5end_porphobilinogen_synthase_(delta-aminolevulinic_acid_dehydratase)_788323..788553_POSITIVE 257 68 21 128 70 aact:AA01600|5end_phosphotransferase_system_enzyme_I_1080481..1080711_POSITIVE 252 136 102 112 72 aact:AA02892|5end_oligopeptide_ABC_transporter,_periplasmic-binding_protein_2026402..2026632_POSITIVE 252 85 56 195 166 aact:AA02024|5end_acyl_carrier_protein_phosphodiesterase_1378144..1378374_POSITIVE 249 68 23 179 125 aact:AA03008|5end_GTP-binding_protein_era_homolog_(GTPase)_2100087..2100317_POSITIVE 245 82 44 231 193 aact:AA00505|5end_hypothetical_protein_351561..351791_POSITIVE 244 92 54 40 1 aact:AA01646|5end_hypothetical_protein_1115069..1115299_POSITIVE 232 136 91 106 56 aact:AA00838|5end_UDP-N-acetylmuramoylalanine--D-glutamate_ligase_577522..577752_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 339 94 54 101 58 aact:AA02167|3end_hypothetical_protein_1483474..1483624_POSITIVE 296 97 53 128 85 aact:AA00117|3end_hypothetical_protein_88418..88568_POSITIVE 290 93 56 134 95 aact:AA01089|3end_hypothetical_protein_753597..753747_POSITIVE 284 92 51 75 33 aact:AA01645|3end_ferric_transport_ABC-related_ATP-binding_protein_1115184..1115334_POSITIVE 282 139 91 142 97 aact:AA01311|3end_transcriptional_regulator_889644..889794_POSITIVE 274 91 55 56 20 aact:AA02048|3end_galactitol_utilization_operon_repressor_1395579..1395729_POSITIVE 274 137 92 59 6 aact:AA01678|3end_possible_transcriptional_regulator_1134375..1134525_POSITIVE 266 63 24 50 4 aact:AA02758|3end_3-phosphoshikimate_1-carboxyvinyltransferase_1933150..1933300_POSITIVE 263 99 59 142 94 aact:AA02698|3end_formate_dehydrogenase_H,_selenopolypeptide_subunit_1881425..1881575_POSITIVE 262 92 54 75 37 aact:AA02899|3end_oligopeptide_ABC_transporter,_ATP-binding_protein_2031138..2031288_POSITIVE 252 85 56 86 57 aact:AA02023|3end_NADH_dehydrogenase_1378115..1378265_POSITIVE 247 92 51 80 41 aact:AA02280|3end_conserved_hypothetical_protein_1552767..1552917_POSITIVE 244 90 49 151 109 aact:AA01646|3end_hypothetical_protein_1115262..1115412_POSITIVE 232 136 91 88 38 aact:AA00837|3end_cell_division_protein_W_577584..577734_POSITIVE 224 92 55 63 27 aact:AA00718|3end_ABC-related_ATP-binding_protein;_MRP_protein_494723..494873_POSITIVE 221 84 41 116 73 aact:AA00181|3end_hypothetical_protein_128855..129005_POSITIVE 217 96 56 86 44 aact:AA02305|3end_guanosine-3',5'-bis(diphosphate)3'-_pyrophosphohydrolase_1576978..1577128_POSITIVE 217 63 22 62 20 aact:AA00551|3end_hypothetical_protein_377403..377553_POSITIVE 217 70 22 59 1 aact:AA00398|3end_protein-tyrosine-phosphatase_(PTPase)_268018..268168_POSITIVE 212 123 87 79 43 aact:AA02234|3end_hypothetical_protein_1521522..1521672_POSITIVE