Origin IGS: atacttcacctcattgtgttaaaagttaaaaattggacaacattctatgaaaatttctccctaaaaactgttagcttgatcaaatatttgaacattgctttatttttta .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 taaaaaataaagcaatgttcaaatatttgatcaagctaacagtttttagggagaaattttcatagaatgttgtccaatttttaacttttaacacaatgaggtgaagtat Mask Tandem Repeat Region ================================================ atacttcacctcattgtgttaaaagttaaaaattggacaacattctatgaaaatttctccctaaaaactgttagcttgatcaaatatttgaacattgctttatttttta Find is-nt database================================================ Query_seq: AA00964:AA00965|AA00964:AA00965:phosphoenolpyruvate carboxykinase/carboxylase:hypothetical protein:->->:674429..674537 109 atacttcacctcattgtgttaaaagttaaaaattggacaacattctatgaaaatttctccctaaaaactgttagcttgatcaaatatttgaacattgctttatttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00964:AA00965|AA00964:AA00965:phosphoenolpyruvate carboxykinase/carboxylase:hypothetical protein:->->:674429..674537 109 atacttcacctcattgtgttaaaagttaaaaattggacaacattctatgaaaatttctccctaaaaactgttagcttgatcaaatatttgaacattgctttatttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00964:AA00965|AA00964:AA00965:phosphoenolpyruvate carboxykinase/carboxylase:hypothetical protein:->->:674429..674537 109 atacttcacctcattgtgttaaaagttaaaaattggacaacattctatgaaaatttctccctaaaaactgttagcttgatcaaatatttgaacattgctttatttttta Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 atacttcacctcattgtgttaaaagttaaaaattggacaacattctatgaaaatttctccctaaaaactgttagcttgatcaaatatttgaacattgctttatttttta Predict ORF larger than 30AA ================================================ Protein_Len: 36 Strand: + Start: 1 End: 108 M L H L I V L K V K N W T T F Y E N F S L K T V S L I K Y L N I A L F F . atacttcacctcattgtgttaaaagttaaaaattggacaacattctatgaaaatttctccctaaaaactgttagcttgatcaaatatttgaacattgctttatttttta Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ atacttcacctcattgtgttaaaagttaaaaattggacaacattctatgaaaatttctccctaaaaactgttagcttgatcaaatatttgaacattgctttatttttta Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00964:AA00965|AA00964:AA00965:phosphoenolpyruvate carboxykinase/carboxylase:hypothetical protein:->->:674429..674537 109 atacttcacctcattgtgttaaaagttaaaaattggacaacattctatgaaaatttctccctaaaaactgttagcttgatcaaatatttgaacattgctttatttttta Intra-Species Hit: Count: 1 Min: 1 Max: 109 Len: 109 Subject: aact_AA00964_AA00965|phosphoenolpyruvate carboxykinase/carboxylase:hypothetical protein|POSITIVE:POSITIVE|[674429,674537]|109 HSP 1 e-value: 1.0E-55 bit: 216.0 Len: 109 Query Start:1 Query End:109 Subject Strand: POSITIVE Subject Start: 1 Subject End: 109 atacttcacctcattgtgttaaaagttaaaaattggacaacattctatgaaaatttctccctaaaaactgttagcttgatcaaatatttgaacattgctttatttttta atacttcacctcattgtgttaaaagttaaaaattggacaacattctatgaaaatttctccctaaaaactgttagcttgatcaaatatttgaacattgctttatttttta Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUACUUCACCUCAUUGUGUUAAAAGUUAAAAAUUGGACAACAUUCUAUGAAAAUUUCUCCCUAAAAACUGUUAGCUUGAUCAAAUAUUUGAACAUUGCUUUAUUUUUUA ..........((((.(((((....(((........))))))))...))))...........((((((.((..(((.((.((((....)))).))..))).)).)))))) (-10.00) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAAAAAUAAAGCAAUGUUCAAAUAUUUGAUCAAGCUAACAGUUUUUAGGGAGAAAUUUUCAUAGAAUGUUGUCCAAUUUUUAACUUUUAACACAAUGAGGUGAAGUAU ..((((((...(((((((((...............((((......)))).((((...))))...)))))))))...)))))).........(((......)))...... (-12.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 259 82 41 128 89 aact:AA01950|5end_ABC_transporter,_membrane_spanning_protein_1318116..1318346_POSITIVE 202 40 1 130 90 aact:AA01131|5end_conserved_hypothetical_protein_775275..775505_POSITIVE 198 39 8 65 29 aact:AA02913|5end_ribosomal_large_subunit_pseudouridine_synthase_2039491..2039721_POSITIVE 196 70 34 54 18 aact:AA02001|5end_conserved_hypothetical_protein_1359842..1360072_POSITIVE 192 48 2 64 20 aact:AA02671|5end_gluconokinase_1858322..1858552_POSITIVE 192 80 34 200 160 aact:AA01446|5end_conserved_hypothetical_protein_970151..970381_POSITIVE 188 63 33 220 192 aact:AA02417|5end_GTP-binding_protein_1659810..1660040_POSITIVE 188 99 54 43 1 aact:AA01911|5end_hypothetical_protein_1287791..1288021_POSITIVE 188 76 35 82 38 aact:AA01584|5end_host_factor-I_protein_1065589..1065819_POSITIVE 187 90 42 159 113 aact:AA02695|5end_hydrogenase-3_component_G_1878565..1878795_POSITIVE 187 69 37 218 186 aact:AA01925|5end_conserved_hypothetical_protein_1299961..1300191_POSITIVE 184 79 36 115 74 aact:AA00684|5end_adenylosuccinate_synthase_467486..467716_POSITIVE 183 80 33 183 121 aact:AA00892|5end_selenocysteine-specific_elongation_factor_(SelB_translation_factor)_618023..618253_POSITIVE 179 62 35 104 73 aact:AA02069|5end_alkylphosphonate_uptake_protein_1406131..1406361_POSITIVE 177 98 57 117 67 aact:AA00686|5end_hypothetical_protein_468852..469082_POSITIVE 177 100 59 214 186 aact:AA00020|5end_conserved_hypothetical_protein_12799..13029_POSITIVE 175 97 55 140 99 aact:AA01256|5end_hypothetical_protein_852774..853004_POSITIVE 169 100 54 137 93 aact:AA01233|5end_50S_ribosomal_protein_L6_840853..841083_POSITIVE 168 99 69 122 95 aact:AA02563|5end_transketolase_C-terminal_section_1785239..1785469_POSITIVE 166 100 60 176 122 aact:AA02002|5end_hypothetical_protein_1360196..1360426_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 259 82 41 128 89 aact:AA01948|3end_ABC_transporter_permease,_membrane_spanning_protein_1318196..1318346_POSITIVE 198 39 8 84 48 aact:AA02912|3end_hypothetical_protein_2039590..2039740_POSITIVE 192 80 34 76 36 aact:AA01445|3end_hypothetical_protein_970107..970257_POSITIVE 191 46 3 77 35 aact:AA01566|3end_conserved_hypothetical_protein_1052091..1052241_POSITIVE 191 46 3 77 35 aact:AA01261|3end_conserved_hypothetical_protein_856723..856873_POSITIVE 188 76 35 64 20 aact:AA01582|3end_hypothetical_protein_1065651..1065801_POSITIVE 187 69 37 113 81 aact:AA01923|3end_type_III_restriction-modification_system_methylation_subunit_1299936..1300086_POSITIVE 185 99 51 148 104 aact:AA01974|3end_conserved_hypothetical_protein_1336411..1336561_POSITIVE 183 80 33 134 72 aact:AA00890|3end_conserved_hypothetical_protein_618054..618204_POSITIVE 180 75 41 65 30 aact:AA01857|3end_glutamate-ammonia-ligase_adenylyltransferase_1250357..1250507_POSITIVE 174 98 69 46 7 aact:AA00684|3end_adenylosuccinate_synthase_468861..469011_POSITIVE 169 100 54 118 74 aact:AA01232|3end_30S_ribosomal_protein_S8_840914..841064_POSITIVE 168 75 31 94 54 aact:AA02545|3end_conserved_hypothetical_protein_1774221..1774371_POSITIVE 166 100 60 139 85 aact:AA02001|3end_conserved_hypothetical_protein_1360239..1360389_POSITIVE 165 44 7 97 59 aact:AA02136|3end_aspartate_aminotransferase_1460476..1460626_POSITIVE 163 100 58 51 14 aact:AA00992|3end_ribonuclease_BN_694568..694718_POSITIVE 160 49 1 151 109 aact:AA01263|3end_hypothetical_protein_861086..861236_POSITIVE 160 82 58 28 8 aact:AA00163|3end_type_IV_prepilin_peptidase;_type_II_secretion_pathway_related_protein_116772..116922_POSITIVE 159 84 41 39 1 aact:AA01778|3end_aminopeptidase_A/I;_cytosol_aminopeptidase;_leucyl_aminopeptidase_1199965..1200115_POSITIVE 159 100 57 133 84 aact:AA01137|3end_anaerobic_dimethyl_sulfoxide_reductase,_chain_A_781057..781207_POSITIVE