Origin IGS: taataagctaaagcaatttaaaagtaaaatactttttacagacagacattgatcatttataacgttgaatgcgattttatgtgctgcacttttaaattcagctctattgataagataattgtatttttcatttgtaaattcaaaaagatagactaaagcctttaccaaagacaccttccctgttcttgctaacttttttatgtttcaacccttcgaaaaaaacattagcagcgatttgccaaaaaaatggcagaataaatattaaattatttaacggaacttttactattctttgctgaaatatcgtacaaagcataaatattgcaattatcggtaccggcagcgaatgacattcacttccggcacttctactttgaccacatcactggttttca .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 tgaaaaccagtgatgtggtcaaagtagaagtgccggaagtgaatgtcattcgctgccggtaccgataattgcaatatttatgctttgtacgatatttcagcaaagaatagtaaaagttccgttaaataatttaatatttattctgccatttttttggcaaatcgctgctaatgtttttttcgaagggttgaaacataaaaaagttagcaagaacagggaaggtgtctttggtaaaggctttagtctatctttttgaatttacaaatgaaaaatacaattatcttatcaatagagctgaatttaaaagtgcagcacataaaatcgcattcaacgttataaatgatcaatgtctgtctgtaaaaagtattttacttttaaattgctttagcttatta Mask Tandem Repeat Region ================================================ taataagctaaagcaatttaaaagtaaaatactttttacagacagacattgatcatttataacgttgaatgcgattttatgtgctgcacttttaaattcagctctattgataagataattgtatttttcatttgtaaattcaaaaagatagactaaagcctttaccaaagacaccttccctgttcttgctaacttttttatgtttcaacccttcgaaaaaaacattagcagcgatttgccaaaaaaatggcagaataaatattaaattatttaacggaacttttactattctttgctgaaatatcgtacaaagcataaatattgcaattatcggtaccggcagcgaatgacattcacttccggcacttctactttgaccacatcactggttttca Find is-nt database================================================ Query_seq: AA00942:AA00943|AA00942:AA00943:related to phosphoglycerate mutase:conserved hypothetical protein:->->:654076..654470 395 taataagctaaagcaatttaaaagtaaaatactttttacagacagacattgatcatttataacgttgaatgcgattttatgtgctgcacttttaaattcagctctattgataagataattgtatttttcatttgtaaattcaaaaagatagactaaagcctttaccaaagacaccttccctgttcttgctaacttttttatgtttcaacccttcgaaaaaaacattagcagcgatttgccaaaaaaatggcagaataaatattaaattatttaacggaacttttactattctttgctgaaatatcgtacaaagcataaatattgcaattatcggtaccggcagcgaatgacattcacttccggcacttctactttgaccacatcactggttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00942:AA00943|AA00942:AA00943:related to phosphoglycerate mutase:conserved hypothetical protein:->->:654076..654470 395 taataagctaaagcaatttaaaagtaaaatactttttacagacagacattgatcatttataacgttgaatgcgattttatgtgctgcacttttaaattcagctctattgataagataattgtatttttcatttgtaaattcaaaaagatagactaaagcctttaccaaagacaccttccctgttcttgctaacttttttatgtttcaacccttcgaaaaaaacattagcagcgatttgccaaaaaaatggcagaataaatattaaattatttaacggaacttttactattctttgctgaaatatcgtacaaagcataaatattgcaattatcggtaccggcagcgaatgacattcacttccggcacttctactttgaccacatcactggttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00942:AA00943|AA00942:AA00943:related to phosphoglycerate mutase:conserved hypothetical protein:->->:654076..654470 395 taataagctaaagcaatttaaaagtaaaatactttttacagacagacattgatcatttataacgttgaatgcgattttatgtgctgcacttttaaattcagctctattgataagataattgtatttttcatttgtaaattcaaaaagatagactaaagcctttaccaaagacaccttccctgttcttgctaacttttttatgtttcaacccttcgaaaaaaacattagcagcgatttgccaaaaaaatggcagaataaatattaaattatttaacggaacttttactattctttgctgaaatatcgtacaaagcataaatattgcaattatcggtaccggcagcgaatgacattcacttccggcacttctactttgaccacatcactggttttca Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 taataagctaaagcaatttaaaagtaaaatactttttacagacagacattgatcatttataacgttgaatgcgattttatgtgctgcacttttaaattcagctctattgataagataattgtatttttcatttgtaaattcaaaaagatagactaaagcctttaccaaagacaccttccctgttcttgctaacttttttatgtttcaacccttcgaaaaaaacattagcagcgatttgccaaaaaaatggcagaataaatattaaattatttaacggaacttttactattctttgctgaaatatcgtacaaagcataaatattgcaattatcggtaccggcagcgaatgacattcacttccggcacttctactttgaccacatcactggttttca Predict ORF larger than 30AA ================================================ Protein_Len: 35 Strand: + Start: 49 End: 153 ................................................ M I I Y N V E C D F M C C T F K F S S I D K I I V F F I C K F K K I D .................................................................................................................................................................................................................................................. Protein_Len: 53 Strand: + Start: 138 End: 296 ......................................................................................................................................... M Q K D R L K P L P K T P S L F L L T F L C F N P S K K T L A A I C Q K N G R I N I K L F N G T F T I L C ................................................................................................... Protein_Len: 34 Strand: + Start: 293 End: 394 .................................................................................................................................................................................................................................................................................................... M L K Y R T K H K Y C N Y R Y R Q R M T F T S G T S T L T T S L V F . Protein_Len: 60 Strand: - Start: 143 End: 343 .............................................................................................................................................. G F V G E R N K S V K K H K L G E F F V N A A I Q W F F P L I F I L N N L P V K V I R Q Q F I T C L M F I A I I P V P M .................................................... Protein_Len: 30 Strand: - Start: 60 End: 149 ........................................................... L T S H S K I H Q V K L N L E I S L I I T N K M Q L N L F M ...................................................................................................................................................................................................................................................... Protein_Len: 60 Strand: - Start: 1 End: 201 L L I S K V S L C Q D N I V N F A I K H A A S K F E A R N I L Y N Y K E N T F E F L Y V L A K V L S V K G T R A L K K M .................................................................................................................................................................................................. taataagctaaagcaatttaaaagtaaaatactttttacagacagacattgatcatttataacgttgaatgcgattttatgtgctgcacttttaaattcagctctattgataagataattgtatttttcatttgtaaattcaaaaagatagactaaagcctttaccaaagacaccttccctgttcttgctaacttttttatgtttcaacccttcgaaaaaaacattagcagcgatttgccaaaaaaatggcagaataaatattaaattatttaacggaacttttactattctttgctgaaatatcgtacaaagcataaatattgcaattatcggtaccggcagcgaatgacattcacttccggcacttctactttgaccacatcactggttttca Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ taataagctaaagcaatttaaaagtaaaatactttttacagacagacattgatcatttataacgttgaatgcgattttatgtgctgcacttttaaattcagctctattgataagataattgtatttttcatttgtaaattcaaaaagatagactaaagcctttaccaaagacaccttccctgttcttgctaacttttttatgtttcaacccttcgaaaaaaacattagcagcgatttgccaaaaaaatggcagaataaatattaaattatttaacggaacttttactattctttgctgaaatatcgtacaaagcataaatattgcaattatcggtaccggcagcgaatgacattcacttccggcacttctactttgaccacatcactggttttca Predict TransTerm conf > 70================================================ TransTerm Strand: + Conf: 81 HP_score: -9.1 Tail_Score: -3.60151 Start: 334 End: 366 Full_Region: ATATTGCAATTATCG GTACCGGCAGCGAAT GAC ATTCACTTCCGGCAC TTCTACTTTGACCAC .............................................................................................................................................................................................................................................................................................................................................GTACCGGCAGCGAATGACATTCACTTCCGGCAC............................. Find igs database================================================ Query_seq: AA00942:AA00943|AA00942:AA00943:related to phosphoglycerate mutase:conserved hypothetical protein:->->:654076..654470 395 taataagctaaagcaatttaaaagtaaaatactttttacagacagacattgatcatttataacgttgaatgcgattttatgtgctgcacttttaaattcagctctattgataagataattgtatttttcatttgtaaattcaaaaagatagactaaagcctttaccaaagacaccttccctgttcttgctaacttttttatgtttcaacccttcgaaaaaaacattagcagcgatttgccaaaaaaatggcagaataaatattaaattatttaacggaacttttactattctttgctgaaatatcgtacaaagcataaatattgcaattatcggtaccggcagcgaatgacattcacttccggcacttctactttgaccacatcactggttttca Intra-Species Hit: Count: 1 Min: 1 Max: 395 Len: 395 Subject: aact_AA00942_AA00943|related to phosphoglycerate mutase:conserved hypothetical protein|POSITIVE:POSITIVE|[654076,654470]|395 HSP 1 e-value: 0.0 bit: 700.0 Len: 395 Query Start:1 Query End:395 Subject Strand: POSITIVE Subject Start: 1 Subject End: 395 taataagctaaagcaatttaaaagtaaaatactttttacagacagacattgatcatttataacgttgaatgcgattttatgtgctgcacttttaaattcagctctattgataagataattgtatttttcatttgtaaattcaaaaagatagactaaagcctttaccaaagacaccttccctgttcttgctaacttttttatgtttcaacccttcgnnnnnnncattagcagcgatttgccnnnnnnntggcagaataaatattaaattatttaacggaacttttactattctttgctgaaatatcgtacaaagcataaatattgcaattatcggtaccggcagcgaatgacattcacttccggcacttctactttgaccacatcactggttttca taataagctaaagcaatttaaaagtaaaatactttttacagacagacattgatcatttataacgttgaatgcgattttatgtgctgcacttttaaattcagctctattgataagataattgtatttttcatttgtaaattcaaaaagatagactaaagcctttaccaaagacaccttccctgttcttgctaacttttttatgtttcaacccttcgaaaaaaacattagcagcgatttgccaaaaaaatggcagaataaatattaaattatttaacggaacttttactattctttgctgaaatatcgtacaaagcataaatattgcaattatcggtaccggcagcgaatgacattcacttccggcacttctactttgaccacatcactggttttca Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UAAUAAGCUAAAGCAAUUUAAAAGUAAAAUACUUUUUACAGACAGACAUUGAUCAUUUAUAACGUUGAAUGCGAUUUUAUGUGCUGCACUUUUAAAUUCAGCUCUAUUGAUAAGAUAAUUGUAUUUUUCAUUUGUAAAUUCAAAAAGAUAGACUAAAGCCUUUACCAAAGACACCUUCCCUGUUCUUGCUAACUUUUUUAUGUUUCAACCCUUCGAAAAAAACAUUAGCAGCGAUUUGCCAAAAAAAUGGCAGAAUAAAUAUUAAAUUAUUUAACGGAACUUUUACUAUUCUUUGCUGAAAUAUCGUACAAAGCAUAAAUAUUGCAAUUAUCGGUACCGGCAGCGAAUGACAUUCACUUCCGGCACUUCUACUUUGACCACAUCACUGGUUUUCA (((((((((...(.(((((((((((((((....)))))).(.(((((((.(((((((((......)))))..))))..)))).))))..)))))))))))))).)))))....((((((((((.(((...((((((..((((.((((((((......(.(((.....))).).........(((((((((((..(((((....(((........))))))))..))))))....(((((((......))))))).((((((......))))))..))))).....))).)))))..))))......))))))....)))...)))))))))).((.((((.((.((((...)))).)).)))).)).........(((((......))))).... (-73.90) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UGAAAACCAGUGAUGUGGUCAAAGUAGAAGUGCCGGAAGUGAAUGUCAUUCGCUGCCGGUACCGAUAAUUGCAAUAUUUAUGCUUUGUACGAUAUUUCAGCAAAGAAUAGUAAAAGUUCCGUUAAAUAAUUUAAUAUUUAUUCUGCCAUUUUUUUGGCAAAUCGCUGCUAAUGUUUUUUUCGAAGGGUUGAAACAUAAAAAAGUUAGCAAGAACAGGGAAGGUGUCUUUGGUAAAGGCUUUAGUCUAUCUUUUUGAAUUUACAAAUGAAAAAUACAAUUAUCUUAUCAAUAGAGCUGAAUUUAAAAGUGCAGCACAUAAAAUCGCAUUCAACGUUAUAAAUGAUCAAUGUCUGUCUGUAAAAAGUAUUUUACUUUUAAAUUGCUUUAGCUUAUUA .......(((.((((((((((........(((((((.(((((((...))))))).))))))).((....(((.((.((((((..((((((.....((((((........(((((.((((...((((((......))))))...(((((......)))))....((((((.(((((((..((....))..))))))).....)).))))..)))).(((((((((.((..(((....)))..)).).))))))))...)))))..................(((.......))))))))).......))))))..)))))))).))).))...........))))).)))))...)))...((((((.((((....)))).))))))......... (-88.40) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 350 376 331 211 165 aact:AA01421|5end_phosphoglycolate_phosphatase_955055..955285_POSITIVE 332 388 342 224 187 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 317 365 328 220 172 aact:AA02362|5end_conserved_hypothetical_protein_1619905..1620135_POSITIVE 311 346 305 87 48 aact:AA00652|5end_arginyl-tRNA_synthetase_(arginine--tRNA_ligase)_445718..445948_POSITIVE 310 362 321 69 30 aact:AA01820|5end_high-affinity_zinc_uptake_system_ATP-binding_protein_1225103..1225333_POSITIVE 302 367 323 57 6 aact:AA00229|5end_DNA_polymerase_III,_alpha_subunit_156863..157093_POSITIVE 301 377 334 140 98 aact:AA01426|5end_threonine_deaminase_(dehydratase)_958207..958437_POSITIVE 300 366 331 228 191 aact:AA02137|5end_transcriptional_regulators_(TetR/AcrR_family)_1460453..1460683_POSITIVE 299 389 343 71 30 aact:AA01845|5end_gamma-glutamyltranspeptidase_precursor_1239841..1240071_POSITIVE 296 362 329 230 198 aact:AA00086|5end_2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy_late_synthase_63437..63667_POSITIVE 289 380 331 225 168 aact:AA02415|5end_hypothetical_protein_1658443..1658673_POSITIVE 289 377 337 118 79 aact:AA02351|5end_conserved_hypothetical_protein_1609233..1609463_POSITIVE 287 340 292 64 18 aact:AA01517|5end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1020989..1021219_POSITIVE 287 390 343 166 106 aact:AA01336|5end_hypothetical_protein_904056..904286_POSITIVE 284 364 321 140 98 aact:AA02727|5end_hypothetical_protein_1909735..1909965_POSITIVE 283 379 331 75 21 aact:AA00164|5end_dephospho-CoA_kinase_(dephosphocoenzyme_A_kinase)_116745..116975_POSITIVE 283 367 327 161 121 aact:AA00039|5end_cytochrome_c-type_biogenesis_protein_30312..30542_POSITIVE 282 89 43 183 136 aact:AA01265|5end_hypothetical_protein_861979..862209_POSITIVE 282 89 43 67 20 aact:AA01264|5end_conserved_hypothetical_protein_861863..862093_POSITIVE 281 388 341 111 62 aact:AA02936|5end_lipoprotein_E_precursor;_outer_membrane_protein_P4_precursor;_acid_phosphatase_2055610..2055840_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 312 364 324 64 27 aact:AA00211|3end_hypothetical_protein_146163..146313_POSITIVE 301 377 334 86 44 aact:AA01424|3end_threonine_deaminase_(dehydratase)_958233..958383_POSITIVE 295 363 331 149 110 aact:AA02411|3end_cysteine_synthetase_1657504..1657654_POSITIVE 287 390 343 139 79 aact:AA01335|3end_primosomal_protein_N'_904109..904259_POSITIVE 284 364 321 47 5 aact:AA02726|3end_anaerobic_C4-dicarboxylate_transporter_1909722..1909872_POSITIVE 284 376 331 88 31 aact:AA00534|3end_hypothetical_protein_365594..365744_POSITIVE 282 346 303 148 106 aact:AA00661|3end_outer_membrane_protein_454531..454681_POSITIVE 278 380 333 75 24 aact:AA01654|3end_periplasmic_maltose-binding_protein_precursor_1121163..1121313_POSITIVE 272 239 194 136 84 aact:AA02947|3end_riboflavin_biosynthesis_protein_2061874..2062024_POSITIVE 272 362 321 132 94 aact:AA01446|3end_conserved_hypothetical_protein_970326..970476_POSITIVE 271 370 321 131 55 aact:AA01818|3end_high-affinity_zinc_uptake_system_membrane_protein_1225078..1225228_POSITIVE 269 379 332 39 1 aact:AA02205|3end_short_chain_dehydrogenase/reductase_1501984..1502134_POSITIVE 269 369 321 120 66 aact:AA01441|3end_tRNA/rRNA_methylase_967643..967793_POSITIVE 268 386 358 126 96 aact:AA00909|3end_molybdopterin-guanine_dinucleotide_biosynthesis_protein_B_627183..627333_POSITIVE 267 188 143 134 79 aact:AA01772|3end_shikimate_5-dehydrogenase_protein_1194942..1195092_POSITIVE 264 388 350 133 101 aact:AA01796|3end_branched-chain_amino_acid_aminotransferase_1212168..1212318_POSITIVE 264 379 333 70 21 aact:AA01289|3end_hypothetical_protein_872848..872998_POSITIVE 264 364 336 66 37 aact:AA01202|3end_integrase/recombinase_824207..824357_POSITIVE 262 369 328 49 3 aact:AA00713|3end_chaperone_protein_hscA,_heat_shock_cognate_66_490697..490847_POSITIVE 261 365 322 121 72 aact:AA01069|3end_translation_elongation_factor_(EF-Ts)_740700..740850_POSITIVE