Origin IGS: ataattccattaaattattttatgatccttataggataatacaattattcgtcatgaaatgaaatcactataatcggcattgacagatcactataaggagttataggtt .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 aacctataactccttatagtgatctgtcaatgccgattatagtgatttcatttcatgacgaataattgtattatcctataaggatcataaaataatttaatggaattat Mask Tandem Repeat Region ================================================ ataattccattaaattattttatgatccttataggataatacaattattcgtcatgaaatgaaatcactataatcggcattgacagatcactataaggagttataggtt Find is-nt database================================================ Query_seq: AA00940:AA00941|AA00940:AA00941:probable transcriptional regulator (LysR family):symporter transmembrane protein:->->:651889..651997 109 ataattccattaaattattttatgatccttataggataatacaattattcgtcatgaaatgaaatcactataatcggcattgacagatcactataaggagttataggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00940:AA00941|AA00940:AA00941:probable transcriptional regulator (LysR family):symporter transmembrane protein:->->:651889..651997 109 ataattccattaaattattttatgatccttataggataatacaattattcgtcatgaaatgaaatcactataatcggcattgacagatcactataaggagttataggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00940:AA00941|AA00940:AA00941:probable transcriptional regulator (LysR family):symporter transmembrane protein:->->:651889..651997 109 ataattccattaaattattttatgatccttataggataatacaattattcgtcatgaaatgaaatcactataatcggcattgacagatcactataaggagttataggtt Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ataattccattaaattattttatgatccttataggataatacaattattcgtcatgaaatgaaatcactataatcggcattgacagatcactataaggagttataggtt Predict ORF larger than 30AA ================================================ ataattccattaaattattttatgatccttataggataatacaattattcgtcatgaaatgaaatcactataatcggcattgacagatcactataaggagttataggtt Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ataattccattaaattattttatgatccttataggataatacaattattcgtcatgaaatgaaatcactataatcggcattgacagatcactataaggagttataggtt Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00940:AA00941|AA00940:AA00941:probable transcriptional regulator (LysR family):symporter transmembrane protein:->->:651889..651997 109 ataattccattaaattattttatgatccttataggataatacaattattcgtcatgaaatgaaatcactataatcggcattgacagatcactataaggagttataggtt Intra-Species Hit: Count: 1 Min: 1 Max: 109 Len: 109 Subject: aact_AA00940_AA00941|probable transcriptional regulator (LysR family):symporter transmembrane protein|POSITIVE:POSITIVE|[651889,651997]|109 HSP 1 e-value: 1.0E-55 bit: 216.0 Len: 109 Query Start:1 Query End:109 Subject Strand: POSITIVE Subject Start: 1 Subject End: 109 ataattccattaaattattttatgatccttataggataatacaattattcgtcatgaaatgaaatcactataatcggcattgacagatcactataaggagttataggtt ataattccattaaattattttatgatccttataggataatacaattattcgtcatgaaatgaaatcactataatcggcattgacagatcactataaggagttataggtt Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAUUCCAUUAAAUUAUUUUAUGAUCCUUAUAGGAUAAUACAAUUAUUCGUCAUGAAAUGAAAUCACUAUAAUCGGCAUUGACAGAUCACUAUAAGGAGUUAUAGGUU ((((((((......((((((((((((.......(((((((...)))))))))))))))))))..................(((....)))......))))))))..... (-18.71) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AACCUAUAACUCCUUAUAGUGAUCUGUCAAUGCCGAUUAUAGUGAUUUCAUUUCAUGACGAAUAAUUGUAUUAUCCUAUAAGGAUCAUAAAAUAAUUUAAUGGAAUUAU ..........(((((((((.(((.....(((((.((((((.((((.......)))).....))))))))))))))))))))))).......(((((((....))))))) (-19.70) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 261 98 51 215 166 aact:AA01807|5end_electron_transport_complex_protein_1216696..1216926_POSITIVE 250 90 49 68 25 aact:AA00921|5end_conserved_hypothetical_protein_633332..633562_POSITIVE 248 79 33 119 67 aact:AA03011|5end_inner_membrane_protein,_60_kDa_2102585..2102815_POSITIVE 248 78 33 120 69 aact:AA02697|5end_hydrogenase_3_maturation_protease_1879870..1880100_POSITIVE 245 83 41 207 169 aact:AA01255|5end_hypothetical_protein_852656..852886_POSITIVE 227 100 59 124 80 aact:AA02790|5end_NAD-dependent_aldehyde_dehydrogenase_1957871..1958101_POSITIVE 226 89 48 58 18 aact:AA02044|5end_phosphotransferase_system_enzyme_II,_galactitol_specific,_protein_A_1391488..1391718_POSITIVE 225 82 46 136 104 aact:AA02970|5end_tyrosine_phenol-lyase_2076373..2076603_POSITIVE 224 100 71 197 165 aact:AA01106|5end_conserved_hypothetical_protein_761675..761905_POSITIVE 214 83 41 75 30 aact:AA02305|5end_guanosine-3',5'-bis(diphosphate)3'-_pyrophosphohydrolase_1574778..1575008_POSITIVE 214 81 44 223 191 aact:AA01681|5end_molybdate-binding_periplasmic_protein_1135159..1135389_POSITIVE 211 89 54 61 13 aact:AA02046|5end_PTS_system_galactitol-specific_enzyme_IIC_1392246..1392476_POSITIVE 211 87 51 211 171 aact:AA00492|5end_conserved_hypothetical_protein_343578..343808_POSITIVE 209 98 61 39 3 aact:AA01843|5end_sensor_protein;_probable_two-component_system_sensor_histidine_kinase_1238377..1238607_POSITIVE 205 92 56 68 36 aact:AA01517|5end_peptidyl-prolyl_cis-trans_isomerase;_macrophage_infectivity_protein_1020989..1021219_POSITIVE 203 90 51 174 130 aact:AA01249|5end_conserved_hypothetical_protein_(possible_phosphoesterase)_849987..850217_POSITIVE 201 67 27 212 165 aact:AA02674|5end_formate_dehydrogenase-related_protein_1859960..1860190_POSITIVE 199 79 50 53 28 aact:AA02495|5end_conserved_hypothetical_protein_1731562..1731792_POSITIVE 198 82 50 75 50 aact:AA02695|5end_hydrogenase-3_component_G_1878565..1878795_POSITIVE 197 88 43 57 7 aact:AA01589|5end_conserved_hypothetical_protein_1070300..1070530_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 248 78 33 127 76 aact:AA02696|3end_formate_hydrogenlyase_maturation_protein_1879957..1880107_POSITIVE 243 92 63 81 50 aact:AA01449|3end_prophage_CP4-57_integrase_971569..971719_POSITIVE 226 89 48 77 37 aact:AA02043|3end_tagatose_6-phosphate_kinase_1391587..1391737_POSITIVE 225 82 46 100 68 aact:AA02969|3end_beta_tyrosinase_(tyrosine_phenol-lyase)_2076417..2076567_POSITIVE 224 100 71 84 52 aact:AA01104|3end_conserved_hypothetical_protein_761642..761792_POSITIVE 217 98 51 49 7 aact:AA01842|3end_conserved_hypothetical_protein_1238467..1238617_POSITIVE 214 83 41 71 26 aact:AA02303|3end_ATP-dependent_DNA_helicase_1574854..1575004_POSITIVE 211 89 54 52 4 aact:AA02045|3end_PTS_system,_galactitol-specific_IIB_component_1392317..1392467_POSITIVE 211 87 51 46 6 aact:AA00491|3end_haemin_storage_system_343493..343643_POSITIVE 205 92 56 33 1 aact:AA01515|3end_SlyX_family_protein_1021034..1021184_POSITIVE 203 90 51 51 7 aact:AA01247|3end_hypothetical_protein_849944..850094_POSITIVE 200 100 59 121 91 aact:AA01390|3end_LexA_repressor_937759..937909_POSITIVE 198 82 50 75 50 aact:AA02693|3end_hydrogenase-4_component_H_1878645..1878795_POSITIVE 197 88 43 57 7 aact:AA01588|3end_N-acetylmuramoyl-L-alanine_amidase_II_1070380..1070530_POSITIVE 196 78 31 75 15 aact:AA01730|3end_conserved_hypothetical_protein_(possible_transcriptional_regulator)_1169083..1169233_POSITIVE 191 90 42 133 93 aact:AA01559|3end_RecA_regulatory_protein,_RecX_1046505..1046655_POSITIVE 190 98 66 149 114 aact:AA02386|3end_hypothetical_protein_1634730..1634880_POSITIVE 187 86 48 54 19 aact:AA01182|3end_3-oxoacyl-(acyl-carrier-protein)_synthase_810510..810660_POSITIVE 185 86 45 125 88 aact:AA01009|3end_universal_stress_protein_A_704945..705095_POSITIVE 185 90 48 96 56 aact:AA00109|3end_conserved_hypothetical_protein_79153..79303_POSITIVE