Origin IGS: ataaaacactccctgatttaagactgaataatttgtttcttgacttgaaaccgctttagtttactgagattcaatgttgaaaactgtgaactttctcccattttttgcataaaaaataatctatcctatgagctactatttagctgaaacggtggaggttaa .........1.........2.........3.........4.........5.........6.........7.........8.........9.........1.........2.........3.........4.........5.........6.........7.........8.........9 ttaacctccaccgtttcagctaaatagtagctcataggatagattattttttatgcaaaaaatgggagaaagttcacagttttcaacattgaatctcagtaaactaaagcggtttcaagtcaagaaacaaattattcagtcttaaatcagggagtgttttat Mask Tandem Repeat Region ================================================ ataaaacactccctgatttaagactgaataatttgtttcttgacttgaaaccgctttagtttactgagattcaatgttgaaaactgtgaactttctcccattttttgcataaaaaataatctatcctatgagctactatttagctgaaacggtggaggttaa Find is-nt database================================================ Query_seq: AA00932:AA00933|AA00932:AA00933:ribose ABC transporter, periplasmic protein:ribose ABC transporter, ATP-binding protein:->->:644256..644417 162 ataaaacactccctgatttaagactgaataatttgtttcttgacttgaaaccgctttagtttactgagattcaatgttgaaaactgtgaactttctcccattttttgcataaaaaataatctatcctatgagctactatttagctgaaacggtggaggttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find is-aa database================================================ Query_seq: AA00932:AA00933|AA00932:AA00933:ribose ABC transporter, periplasmic protein:ribose ABC transporter, ATP-binding protein:->->:644256..644417 162 ataaaacactccctgatttaagactgaataatttgtttcttgacttgaaaccgctttagtttactgagattcaatgttgaaaactgtgaactttctcccattttttgcataaaaaataatctatcctatgagctactatttagctgaaacggtggaggttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 Find nr database================================================ Query_seq: AA00932:AA00933|AA00932:AA00933:ribose ABC transporter, periplasmic protein:ribose ABC transporter, ATP-binding protein:->->:644256..644417 162 ataaaacactccctgatttaagactgaataatttgtttcttgacttgaaaccgctttagtttactgagattcaatgttgaaaactgtgaactttctcccattttttgcataaaaaataatctatcctatgagctactatttagctgaaacggtggaggttaa Intra-Species Hit: Count: 0 Inter-species Hit: Count: 0 ataaaacactccctgatttaagactgaataatttgtttcttgacttgaaaccgctttagtttactgagattcaatgttgaaaactgtgaactttctcccattttttgcataaaaaataatctatcctatgagctactatttagctgaaacggtggaggttaa Predict ORF larger than 30AA ================================================ Protein_Len: 30 Strand: - Start: 21 End: 110 .................... S Q I I Q K K V Q F R K L K S L N L T S F Q S S E G M K Q M .................................................... ataaaacactccctgatttaagactgaataatttgtttcttgacttgaaaccgctttagtttactgagattcaatgttgaaaactgtgaactttctcccattttttgcataaaaaataatctatcctatgagctactatttagctgaaacggtggaggttaa Predict Promoter with matrix: RpoD-15 score > 80================================================ Predict Promoter with matrix: RpoD-16 score > 80================================================ Predict Promoter with matrix: RpoD-17 score > 80================================================ Predict Promoter with matrix: RpoD-18 score > 80================================================ Predict Promoter with matrix: RpoD-19 score > 80================================================ Predict Promoter with matrix: RpoN score > 80================================================ ataaaacactccctgatttaagactgaataatttgtttcttgacttgaaaccgctttagtttactgagattcaatgttgaaaactgtgaactttctcccattttttgcataaaaaataatctatcctatgagctactatttagctgaaacggtggaggttaa Predict TransTerm conf > 70================================================ Find igs database================================================ Query_seq: AA00932:AA00933|AA00932:AA00933:ribose ABC transporter, periplasmic protein:ribose ABC transporter, ATP-binding protein:->->:644256..644417 162 ataaaacactccctgatttaagactgaataatttgtttcttgacttgaaaccgctttagtttactgagattcaatgttgaaaactgtgaactttctcccattttttgcataaaaaataatctatcctatgagctactatttagctgaaacggtggaggttaa Intra-Species Hit: Count: 1 Min: 1 Max: 162 Len: 162 Subject: aact_AA00932_AA00933|ribose ABC transporter, periplasmic protein:ribose ABC transporter, ATP-binding protein|POSITIVE:POSITIVE|[644256,644417]|162 HSP 1 e-value: 4.0E-87 bit: 321.0 Len: 162 Query Start:1 Query End:162 Subject Strand: POSITIVE Subject Start: 1 Subject End: 162 ataaaacactccctgatttaagactgaataatttgtttcttgacttgaaaccgctttagtttactgagattcaatgttgaaaactgtgaactttctcccattttttgcataaaaaataatctatcctatgagctactatttagctgaaacggtggaggttaa ataaaacactccctgatttaagactgaataatttgtttcttgacttgaaaccgctttagtttactgagattcaatgttgaaaactgtgaactttctcccattttttgcataaaaaataatctatcctatgagctactatttagctgaaacggtggaggttaa Inter-species Hit: Count: 0 Predict Forward Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ AUAAAACACUCCCUGAUUUAAGACUGAAUAAUUUGUUUCUUGACUUGAAACCGCUUUAGUUUACUGAGAUUCAAUGUUGAAAACUGUGAACUUUCUCCCAUUUUUUGCAUAAAAAAUAAUCUAUCCUAUGAGCUACUAUUUAGCUGAAACGGUGGAGGUUAA ....(((.(((((((.(((.((.(((((((....(((((.......))))).((((.(((((((.....((((....))))....))))))).......(((((((....)))))))............))))...))))))))).)))))).))))))).. (-31.40) Predict Reverse Strand Minimun Free Energy by RNAFold (ViennaRNA) ================================================ UUAACCUCCACCGUUUCAGCUAAAUAGUAGCUCAUAGGAUAGAUUAUUUUUUAUGCAAAAAAUGGGAGAAAGUUCACAGUUUUCAACAUUGAAUCUCAGUAAACUAAAGCGGUUUCAAGUCAAGAAACAAAUUAUUCAGUCUUAAAUCAGGGAGUGUUUUAU ..(((((((...((((.((((.((((((.(((..(((.......((((((((....)))))))).((((...((((..((.....))..))))))))......))).))).(((((.......)))))..)))))).)).)).))))...)))).))).... (-27.50) Find mRNA Target Using Conserved IGS================================================ 5'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 259 159 115 85 42 aact:AA00362|5end_30S_ribosomal_protein_S3_244847..245077_POSITIVE 241 69 21 78 33 aact:AA00718|5end_ABC-related_ATP-binding_protein;_MRP_protein_493534..493764_POSITIVE 239 94 59 172 142 aact:AA01764|5end_hypothetical_protein_1191526..1191756_POSITIVE 235 160 118 65 37 aact:AA02902|5end_aerobic_respiration_control_protein_2031171..2031401_POSITIVE 234 158 116 216 180 aact:AA02025|5end_hypothetical_protein_1378667..1378897_POSITIVE 234 97 51 89 45 aact:AA01861|5end_phosphomethylpyrimidine_kinase_1251806..1252036_POSITIVE 229 88 43 227 185 aact:AA01694|5end_PTS_system,_IIA_component_1149929..1150159_POSITIVE 228 109 75 158 128 aact:AA01995|5end_conserved_hypothetical_protein_(possible_chorismate_mutase)_1355120..1355350_POSITIVE 224 99 51 78 37 aact:AA01681|5end_molybdate-binding_periplasmic_protein_1135159..1135389_POSITIVE 223 89 41 66 16 aact:AA01224|5end_CTP_synthase_836221..836451_POSITIVE 222 55 12 200 150 aact:AA02325|5end_membrane_protein;_possible_ABC_transporter,_permease_1587309..1587539_POSITIVE 220 60 14 58 5 aact:AA01237|5end_preprotein_translocase_842897..843127_POSITIVE 220 158 128 221 186 aact:AA00784|5end_hypothetical_protein_539025..539255_POSITIVE 218 106 64 170 121 aact:AA01544|5end_conserved_hypothetical_protein_1034265..1034495_POSITIVE 218 106 64 80 31 aact:AA01543|5end_hypothetical_protein_1034175..1034405_POSITIVE 217 159 137 57 37 aact:AA01201|5end_phosphatase;_hydrolase_822513..822743_POSITIVE 215 80 31 194 146 aact:AA01724|5end_valyl-tRNA_synthetase_1164446..1164676_POSITIVE 215 159 116 228 186 aact:AA01674|5end_hypothetical_protein_1132017..1132247_POSITIVE 214 80 41 138 92 aact:AA01294|5end_DNA_adenine_methylase_876415..876645_POSITIVE 214 56 22 167 119 aact:AA00050|5end_cytochrome_c-type_biogenesis_protein_precursor_(reductase)_39919..40149_POSITIVE 3'END mRNA Target Prediction=================================== Score srna_start srna_end target_start tegart_end seq_id 259 159 115 68 25 aact:AA00361|3end_50S_ribosomal_protein_L16_244910..245060_POSITIVE 234 97 51 93 49 aact:AA01859|3end_hypothetical_protein_1251890..1252040_POSITIVE 234 127 81 149 109 aact:AA00020|3end_conserved_hypothetical_protein_14486..14636_POSITIVE 229 88 43 147 105 aact:AA01692|3end_hexulose-6-phosphate_synthase_1149929..1150079_POSITIVE 224 99 51 75 34 aact:AA01679|3end_aspartate-semialdehyde_dehydrogenase;_PTS_system_enzyme_II_A_component_1135236..1135386_POSITIVE 219 60 16 51 1 aact:AA01236|3end_50S_ribosomal_protein_L15_842970..843120_POSITIVE 214 66 33 148 113 aact:AA00644|3end_lipoprotein_441371..441521_POSITIVE 211 70 21 94 32 aact:AA01579|3end_transcriptional_regulatory_protein_1065283..1065433_POSITIVE 211 77 35 143 101 aact:AA01243|3end_50S_ribosomal_protein_L17_847440..847590_POSITIVE 209 90 41 57 12 aact:AA02464|3end_hypothetical_protein_1704094..1704244_POSITIVE 209 160 118 141 95 aact:AA02363|3end_hflK_protein_1622074..1622224_POSITIVE 208 160 119 130 78 aact:AA00093|3end_L-serine_dehydratase_(L-serine_deaminase)_71173..71323_POSITIVE 207 98 51 99 53 aact:AA01756|3end_cobalt_membrane_transport_protein_1187217..1187367_POSITIVE 207 100 51 131 81 aact:AA00970|3end_uroporphyrinogen_decarboxylase_(uroporphyrinogen_III_decarboxylase)_677267..677417_POSITIVE 205 89 48 151 106 aact:AA00785|3end_conserved_hypothetical_protein_(possible_integrase)_539672..539822_POSITIVE 202 156 113 126 90 aact:AA00092|3end_serine_transporter_69716..69866_POSITIVE 200 160 124 133 105 aact:AA02205|3end_short_chain_dehydrogenase/reductase_1501984..1502134_POSITIVE 199 160 129 128 92 aact:AA02777|3end_tRNA-guanine_transglycosylase_1942527..1942677_POSITIVE 199 55 12 106 55 aact:AA02171|3end_polysialic_acid_capsule_expression_protein_1486096..1486246_POSITIVE 199 100 51 104 62 aact:AA00198|3end_conserved_hypothetical_protein_(Cytosine_deaminase-related_enzyme)_138643..138793_POSITIVE